Physical mapping of the 5S rRNA multigene family in 6x triticale and rye: identification of a new rye locus

Genome ◽  
1995 ◽  
Vol 38 (3) ◽  
pp. 623-626 ◽  
Author(s):  
Angeles Cuadrado ◽  
Nicolas Jouv ◽  
J. S. Heslop-Harrison
Keyword(s):  
In Situ Hybridization ◽  
Multigene Family ◽  
The Other ◽  
5S Rrna ◽  
Rrna Genes ◽  
Hexaploid Triticale ◽  
Selected Lines ◽  
Secale Cereale L ◽  
5S Rrna Genes

In situ hybridization was used to physically map the 5S rRNA multigene family in three selected lines of hexaploid triticale and five lines of diploid rye. Using this technique, evidence for a new locus on the 3RS arm of the three triticale lines was first obtained, as well as confirmation of the presence of 5S rRNA loci on wheat and rye chromosomes of homoeologous groups 1 and 5. The new locus on the 3RS arm was confirmed in two lines of rye, Secale cereale L., although it was not present in the other rye varieties studied. We propose that the new 5S rRNA locus be referred to as 5SDna-R3.Key words: in situ hybridization, FISH, Secale, triticale, 5S rRNA genes.

Chromosomal mapping of mouse 5S rRNA genes by direct R-banding fluorescence in situ hybridization

1994 ◽  
Vol 66 (4) ◽  
pp. 246-249 ◽  
Author(s):  
Y. Matsuda ◽  
K. Moriwaki ◽  
V.M. Chapman ◽  
Y. Hoi-Sen ◽  
J. Akbarzadeh ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
5S Rrna ◽  
Chromosomal Mapping ◽  
Rrna Genes ◽  
5S Rrna Genes

Organization of the 5S ribosomal RNA genes in the genome of tomato

Genome ◽  
1991 ◽  
Vol 34 (4) ◽  
pp. 509-514 ◽  
Author(s):  
Nora L. V. Lapitan ◽  
Martin W. Ganal ◽  
Steven D. Tanksley
Keyword(s):  
In Situ Hybridization ◽  
Ribosomal Rna ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
5S Rrna ◽  
Chromosome 1 ◽  
Rrna Genes ◽  
5S Rrna Genes

The 5S ribosomal RNA (rRNA) genes represent one of the most abundant gene families in eukaryotic genomes and have been a model system for the study of molecular organization and evolution of multigene families in eukaryotes. This paper reports a detailed characterization of the 5S rRNA genes of tomato (Lycopersicon esculentum) with respect to chromosome assignment, chromosomal localization, copy number, and physical size. By restriction fragment length polymorphism, the tandemly repeated 5S rRNA genes were assigned to a region of chromosome 1 of tomato. These results were confirmed by in situ hybridization onto tomato metaphase chromosomes. The single hybridization signal was localized to the short arm of chromosome 1, in a region close to the centromere. Based on reconstruction experiments, it was estimated that the 400-bp repeating unit occurs in approximately 1000 copies per haploid genome. Physical characterization of the entire locus was then performed by means of pulsed-field gel electrophoresis. Digestion of high molecular weight DNA of tomato with restriction enzymes such as PvuII, ClaI, and BglII resulted in a very prominent band with a size between approximately 450 and 600 kb. This value closely matched the estimated size of the gene cluster based on reconstruction experiments. The data therefore suggest that all the 5S rRNA genes in tomato occur in a single, continuous array, uninterrupted by unrelated sequences.Key words: 5S rRNA genes, organization, Lycopersicon esculentum, in situ hybridization, pulsed-field gel electrophoresis.

scholarly journals Physical Localization of 5S rRNA Genes in the Pig by Fluorescence in Situ Hybridization

Hereditas ◽  
2004 ◽  
Vol 124 (1) ◽  
pp. 95-97 ◽  
Author(s):  
Clemens H. M. Mellink ◽  
Anneke A. Bosma ◽  
Nel A. Haan ◽  
Carla Zijlstra
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
5S Rrna ◽  
Rrna Genes ◽  
5S Rrna Genes

Genetic and chromosomal localization of the 5S rDNA locus in sugar beet (Beta vulgaris L.)

Genome ◽  
1997 ◽  
Vol 40 (2) ◽  
pp. 171-175 ◽  
Author(s):  
J. Schondelmaier ◽  
T. Schmidt ◽  
C. Jung ◽  
J. S. Heslop-Harrison
Keyword(s):  
In Situ Hybridization ◽  
Linkage Group ◽  
Sugar Beet ◽  
Beta Vulgaris ◽  
5S Rdna ◽  
Rdna Locus ◽  
5S Rrna ◽  
Rrna Genes ◽  
Rrna Gene

A digoxigenin-labelled 5S rDNA probe containing the 5S rRNA gene and the adjacent intergenic spacer was used for in situ hybridization to metaphase and interphase chromosomes of a trisomic stock from sugar beet (Beta vulgaris L.). Three chromosomes of primary trisomic line IV (T. Butterfass. Z. Bot. 52: 46–77. 1964) revealed signals close to the centromeres. Polymorphisms of 5S rDNA repeats in a segregating population were used to map genetically the 5S rRNA genes within a cluster of markers in linkage group II of sugar beet. The concentration of genetic markers around the centromere presumably reflects the suppressed recombination frequency in centromeric regions. The correlation of physical and genetic data allowed the assignment of a linkage group to sugar beet chromosome IV according to line IV of the primary trisomics.Key words: Beta vulgaris, sugar beet, 5S rRNA, in situ hybridization, RFLPs, trisomics.

Chromosomal mapping of 18S–28S and 5S rRNA genes by two-colour fluorescent in situ hybridization in six sturgeon species

Genome ◽  
2003 ◽  
Vol 46 (3) ◽  
pp. 473-477 ◽  
Author(s):  
Francesco Fontana ◽  
Massimo Lanfredi ◽  
Leonardo Congiu ◽  
Marilena Leis ◽  
Milvia Chicca ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Close Association ◽  
5S Rdna ◽  
28S Rdna ◽  
5S Rrna ◽  
Chromosomal Mapping ◽  
Rrna Genes ◽  
Acipenser Baerii ◽  
Sturgeon Species

The number and distribution of the 18S–28S and 5S rRNA (rDNA) gene sequences were examined on mitotic chromosomes of six sturgeon species by two-colour in situ hybridization. Four of the six species, Huso huso, Acipenser stellatus, Acipenser sturio, and Acipenser ruthenus, with about 120 chromosomes, showed from six to eight 18S–28S rDNA signals, while 5S rDNA signals were on only one chromosome pair. The two species with 250–270 chromosomes, Acipenser baerii and Acipenser transmontanus, showed from 10 to 12 18S–28S sites and two chromosome pairs bearing 5S rDNA signals. In all examined species, the rather intense 5S rDNA signals apparently overlapped those of 18S–28S rDNA. These data support the diploid–tetraploid relationships between the two chromosome groups of sturgeons. The close association between the two rDNA families in species belonging to an ancestral fish order, such as Acipenseriformes, supports the hypothesis that the association represents a primitive condition.Key words: Acipenseriformes, FISH, fish cytogenetics, ribosomal genes.

Divergent Evolution of 5 S rRNA Genes in Methanococcus

1987 ◽  
Vol 42 (4) ◽  
pp. 373-380 ◽  
Author(s):  
Günter Wich ◽  
Lionel Sibold ◽  
August Böck
Keyword(s):  
Sequence Divergence ◽  
The Other ◽  
5S Rrna ◽  
Rrna Genes ◽  
Transcriptional Unit ◽  
Divergent Evolution ◽  
Trna Genes ◽  
Rrna Gene ◽  
Gene Comparison ◽  
5S Rrna Genes

Abstract The organization of genes for 5S rRNA in the methanogenic archaebacterium Methanococcus (M .) voltae and their nucleotide sequences have been determined. M. voltae possesses three 5S rRNA genes, one of them is organized in an rRNA transcriptional unit coding for 16S-23S-5S rRNA. The other two are associated with seven tRNA genes in a putative transcriptional unit composed of 5′-tRNAThr-tRNAPro-tRNATyr-tRNALys - 5S rRNA-tRNAAsp-tRNALys - 5S rRNA-tRNAAsp-3′. Coding regions plus spacers of the tRNALys-5S rRNA-tRNAAsp block of this gene cluster occur twice with identical sequence. The 5S rRNA from this cluster displays considerable sequence divergence to the rRNA operon-linked 5S rRNA gene. Comparison of the M. voltae 5S rRNA sequences with those from M. vannielii revealed that the operon-linked genes on one hand and the tRNA-linked 5S genes on the other share a greater sequence homology than the two types of genes within each of the two organisms. This indicates an independent evolution of the two sets of 5S rRNA genes without selective pressure from other ribosomal components or, alternatively, lateral gene transfer.

Cytological and molecular evidence of deletion of ribosomal RNA genes in chromosome 6 of barley (Hordeum vulgare)

Genome ◽  
1994 ◽  
Vol 37 (3) ◽  
pp. 419-425 ◽  
Author(s):  
K. Gecheff ◽  
T. Hvarleva ◽  
S. Georgiev ◽  
T. Wilkes ◽  
A. Karp
Keyword(s):  
In Situ Hybridization ◽  
Organizer Region ◽  
Nucleolus Organizer Region ◽  
Molecular Techniques ◽  
The Other ◽  
Rrna Genes ◽  
Molecular Evidence ◽  
Chromosome 6 ◽  
Giemsa Banding

The barley chromosomal mutant T-35, in which only one pair of satellite chromosomes is apparent, was analyzed using a range of cytological and molecular techniques. Using conventional Feulgen staining, Giemsa and silver banding, in situ hybridization, and Southern blot analysis, unequivocal cytological and molecular evidence was obtained that T-35 is a homozygous deletion of rRNA genes residing in the nucleolus organizer region (NOR) of chromosome 6. According to the criteria of arm ratio and Giemsa-banding pattern of this chromosome, the deletion involved the whole NOR, one of the breakpoints being localized in the short arm proximally to the NOR-associated heterochromatic band, the other probably in the satellite of the chromosome. As a result of this deletion, an increased activity of the rRNA genes (as indicated by the size of the silver bands) on the other NOR-bearing chromosome (chromosome 7) was observed. The possible reasons for this phenomenon are discussed.Key words: barley, nucleolar organizing region, deletion, silver and Giemsa banding, in situ hybridization.

Physical mapping of ribosomal DNA sites in Festuca arundinacea and related species by in situ hybridization

Genome ◽  
1997 ◽  
Vol 40 (3) ◽  
pp. 406-410 ◽  
Author(s):  
H. M. Thomas ◽  
J. A. Harper ◽  
M. R. Meredith ◽  
W. G. Morgan ◽  
I. P. King
Keyword(s):  
In Situ Hybridization ◽  
Ribosomal Dna ◽  
Physical Mapping ◽  
Festuca Arundinacea ◽  
Rrna Genes ◽  
Structural Rearrangements ◽  
Phylogenetic Studies ◽  
Rdna Sites ◽  
5S Rrna Genes

The positions of the 18S–5.8S–26S and 5S rRNA genes have been physically mapped on the chromosomes of diploid, tetraploid, and hexaploid Festuca species by in situ hybridization. The number and position of the rDNA sites in the species were compared. The results confirm some of the earlier phylogenetic studies of these species but suggest that some structural rearrangements have occurred and that sites have been lost during polyploidization. Keywords: Festuca, in situ hybridization, phylogeny, physical mapping, rDNA.

Sign in / Sign up

Export Citation Format

Share Document