The use of cell culture for subchromosomal introgressions of barley yellow dwarf virus resistance from Thinopyrum intermedium to wheat

Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 395-405 ◽  
Author(s):  
P. M. Banks ◽  
P. J. Larkin ◽  
H. S. Bariana ◽  
E. S. Lagudah ◽  
R. Appels ◽  
...  
Keyword(s):  
Cell Culture ◽  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Polymorphism Analysis ◽  
Meiotic Analysis ◽  
Barley Yellow Dwarf ◽  
Wheat Group ◽  
Yellow Dwarf Virus ◽  
Alien Chromatin

Barley yellow dwarf virus (BYDV) resistance has been transferred to wheat from a group 7 chromosome of Thinopyrum (Agropyron) intermedium. The source of the resistance gene was the L1 disomic addition line, which carries the 7Ai-1 chromosome. The resistance locus is on the long arm of this chromosome. BYDV resistant recombinant lines were identified after three or more generations of selection against a group 7 Th. intermedium short arm marker (red coleoptile) and selection for the presence of BYDV resistance. One recombinant line produced by ph. mutant induced homoeologous pairing and 14 recombinant lines induced by cell culture have been identified. Resistance in seven of the cell culture induced recombinants has been inherited via pollen according to Mendelian segregation ratios for up to eight generations. Meiotic analysis of heterozygotes indicates that the alien chromatin in the cell culture induced recombinants is small enough to allow regular meiotic behaviour. The ph-induced recombinant was less regular in meiosis. A probe, pEleAcc2, originally isolated from Th. elongatum and that hybridizes to dispersed repeated DNA sequences, was utilised to detect Th. intermedium chromatin, which confers resistance to BYDV, in wheat backgrounds. Quantification of these hybridization signals indicated that the translocations involved a portion of alien chromatin that was smaller than the complete long arm of 7Ai-1. Restriction fragment length polymorphism analysis confirmed the loss of the short arm of 7Ai-1 and indicated the retention of segments of the long arm of 7Ai-1. Two 7Ai-1L DNA markers always assorted with the BYDV resistance. A third 7Ai-IL DNA marker was also present in seven of eight recombinants. In all recombinants except TC7, the 7Ai-1L markers replaced the 7DL markers. None of the wheat group 7 markers was missing from TC7. It is concluded that all the resistant lines are the result of recombination with wheat chromosome 7D, except line TC7, which is the result of recombination with an unidentified nongroup 7 chromosome.Key words: Triticum, Agropyron, alien genes, translocation, somatic recombination, luteovirus.

Implementation of probes for tracing chromosome segments conferring barley yellow dwarf virus resistance

2001 ◽  
Vol 52 (12) ◽  
pp. 1389 ◽  
Author(s):  
W. Zhang ◽  
M. Carter ◽  
S. Matsay ◽  
P. Stoutjesdijk ◽  
R. Potter ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Barley Yellow Dwarf Virus ◽  
Solid Phase ◽  
Wheat Breeding ◽  
Yellow Dwarf ◽  
Chromosome Segment ◽  
Dwarf Virus ◽  
Barley Yellow Dwarf ◽  
Wheat Group ◽  
Yellow Dwarf Virus

Two PCR-based assays were examined for tracing the presence of a Thinopyrum chromosome segment (Tc6 or Tc14) conferring barley yellow dwarf virus (BYDV) resistance in wheat breeding lines. The microsatellite gwm37 was used to assay the Thinopyrum chromosome segment or its wheat, Group 7, homoeologous segment, and was effective in characterising breeders material since heterozygous lines could be identified. A new set of primers derived from a Thinopyrum-specific DNA segment (csTiB1) provided a dominant marker that was readily scored by agarose gel electrophoresis. It was also demonstrated that the csTiB1 primers could be used to establish a solid phase PCR assay that avoided the requirement for gel electrophoresis and was amenable to use in a high-throughput, microtitre plate format. Depending on the number of DNA samples to be assayed, both primer pairs appear to have a place in breeding programs.

Varying chromosome composition of 56-chromosome wheat × Thinopyrum intermedium partial amphiploids

Genome ◽  
1993 ◽  
Vol 36 (2) ◽  
pp. 207-215 ◽  
Author(s):  
P. M. Banks ◽  
S. J. Xu ◽  
R. R.-C. Wang ◽  
P. J. Larkin
Keyword(s):  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Molecular Evidence ◽  
Thinopyrum Intermedium ◽  
Meiotic Analysis ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus ◽  
Head Morphology ◽  
Partial Amphiploids

Thinopyrum intermedium (2n = 42) is a source of many potentially useful genes for wheat improvement. Many partial amphiploids have been produced between Th. intermedium and Triticum aestivum that are fertile and stable. These partial amphiploids all have 56 chromosomes, including seven pairs of chromosomes from Th. intermedium. To explore the genomic composition of these lines, meiotic analysis was conducted on 32 hybrid combinations between eight different partial amphiploids. All but two of the chosen parents were distinguishable on the basis of perenniality, head morphology, and reactions to leaf, stripe, and stem rusts and to barley yellow dwarf virus. Chromosome pairing in the hybrids clearly indicated that all but two of the partial amphiploids differed in their composition of Thinopyrum chromosomes. The differences varied from one to five chromosomes. This confirms molecular evidence that the extra genome of the octoploid partial amphiploids is a variable synthetic genome combining chromosomes of the three Thinopyrum genomes E, J, and X. Though the extra synthetic genomes vary widely between different octoploids, they are nevertheless stable once formed. It is argued that the failure to establish these octoploid amphiploids as a new crop is a consequence of their differing chromosome complements, which makes it impractical to interbreed them.Key words: Thinopyrum, Agropyron, agrotriticum, wheat, amphiploid, octoploid, barley yellow dwarf virus, rust.

Molecular characterization of a Thinopyrum intermedium Group 2 chromosome (2Ai-2) conferring resistance to barley yellow dwarf virus

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 1129-1135 ◽  
Author(s):  
Z Y Zhang ◽  
Z Y Xin ◽  
P J Larkin
Keyword(s):  
Barley Yellow Dwarf Virus ◽  
Rapd Marker ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Rflp Markers ◽  
Thinopyrum Intermedium ◽  
Barley Yellow Dwarf ◽  
Wheat Group ◽  
Group 2 ◽  
Yellow Dwarf Virus

The wheat – Thinopyrum intermedium addition lines Z1 and Z2 carry 21 pairs of wheat chromosomes and one pair of Th. intermedium chromosomes (2Ai-2) conferring resistance to barley yellow dwarf virus (BYDV). GISH results using the genomic DNA of Pseudoroegneria strigosa (S genome) as the probe indicated that the 2Ai-2 chromosome in Z1 and Z2 is an S–J intercalary translocation. Most of the 2Ai-2 chromosome belongs to the S genome, except for about one third in the middle region of the long arm that belongs to the J genome. The results of detailed RFLP analyses confirmed that the 2Ai-2 chromosome is extensively homoeologous to wheat group 2 chromosomes. Some new RFLP markers specific to the 2Ai-2 chromosome were identified. A RAPD marker, OP-R16340, specific to the 2Ai-2 chromosome, was screened. We converted the RAPD marker into a sequence-characterized amplified region (SCAR) marker (designated SC-R16). The study establishes the basis for selecting translocation lines with small segments of the 2Ai-2 chromosome and localizing the BYDV resistance gene when introgressed into a wheat background.Key words: Thinopyrum intermedium, barley yellow dwarf virus (BYDV), genomic in situ hybridization (GISH), RFLP, SCAR, homoeologous group 2.

Identification and characterization of wheat-wheatgrass translocation lines and localization of barley yellow dwarf virus resistance

Genome ◽  
2000 ◽  
Vol 43 (4) ◽  
pp. 698-706 ◽  
Author(s):  
O R Crasta ◽  
M G Francki ◽  
D B Bucholtz ◽  
H C Sharma ◽  
J Zhang ◽  
...  
Keyword(s):  
Repetitive Sequence ◽  
Barley Yellow Dwarf Virus ◽  
Rflp Analysis ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Thinopyrum Intermedium ◽  
Barley Yellow Dwarf ◽  
Translocation Lines ◽  
Yellow Dwarf Virus ◽  
Alien Chromatin

Stable introgression of agronomically important traits into crop plants through wide crossing often requires the generation and identification of translocation lines. However, the low efficiency of identifying lines containing translocations is a significant limitation in utilizing valuable alien chromatin-derived traits. Selection of putative wheatgrass-wheat translocation lines based on segregation ratios of progeny from γ-irradiated seed using a standard phenotypic analysis resulted in a low 4% success rate of identifying barley yellow dwarf virus (BYDV) resistant and susceptible translocation lines. However, 58% of the susceptible progeny of this irradiated seed contained a Thinopyrum intermedium chromosome-specific repetitive sequence, which indicated that γ-irradiation-induced translocations occurred at high rate. Restriction fragment length polymorphism (RFLP) analysis of susceptible lines containing alien chromatin, their resistant sister lines and other resistant lines showed that more than one third of the progeny of γ-irradiated double monosomic seeds contained wheatgrass-wheat translocations. Genomic in situ hybridization (GISH) analysis of selected lines confirmed that these were wheatgrass-wheat translocation lines. This approach of initially identifying BYDV susceptible deletion lines using an alien chromosome-specific repetitive sequence followed by RFLP analysis of their resistant sister lines efficiently identified resistant translocation lines and localized the BYDV resistance to the distal end of the introgressed Th. intermedium chromosome.Key words: gene introgression, wide crosses, chromosome, repetitive elements, RFLP, Thinopyrum intermedium.

Tolerance to Barley Yellow Dwarf Virus in Triticale

Crop Science ◽  
1990 ◽  
Vol 30 (5) ◽  
pp. 1008 ◽  
Author(s):  
J. Collin ◽  
A. Comeau ◽  
C.A. St-Pierre
Keyword(s):  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus

Modified Recurrent Selection for Barley Yellow Dwarf Virus Tolerance in Winter Wheat

Crop Science ◽  
1994 ◽  
Vol 34 (2) ◽  
pp. 371-375 ◽  
Author(s):  
Ellen M. Bauske ◽  
Frederic L. Kolb ◽  
Adrianna D. Hewings ◽  
Gordon Cisar
Keyword(s):  
Winter Wheat ◽  
Recurrent Selection ◽  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Barley Yellow Dwarf ◽  
Virus Tolerance ◽  
Selection For ◽  
Yellow Dwarf Virus
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