Assessment of the degree and the type of restriction fragment length polymorphism in barley (Hordeum vulgare)

1990 ◽  
Vol 80 (6) ◽  
pp. 826-832 ◽  
Author(s):  
A. Graner ◽  
H. Siedler ◽  
A. Jahoor ◽  
R. G. Herrmann ◽  
G. Wenzel
Keyword(s):  
Hordeum Vulgare ◽  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Restriction Fragment Length

Characterization of Hordeum vulgare – Hordeum bulbosum chromosome substitution lines by restriction fragment length polymorphism analysis

Genome ◽  
1993 ◽  
Vol 36 (3) ◽  
pp. 507-511 ◽  
Author(s):  
Gail M. Timmerman ◽  
Richard A. Pickering ◽  
Gilbert Melz
Keyword(s):  
Hordeum Vulgare ◽  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Molecular Techniques ◽  
Polymorphism Analysis ◽  
Hordeum Bulbosum ◽  
Restriction Fragment Length

Plants obtained from crosses between Hordeum vulgare and H. bulbosum were previously analyzed cytologically and for isozyme composition. They were identified as possessing substitutions of one or more H. vulgare chromosomes by their H. bulbosum homoeologues. To confirm their constitution and assess the merits of molecular techniques, chromosome-specific probes developed for the Triticeae were hybridized to Southern blots of DNA extracted from these plants and their parents. The hybridization patterns in the substitution plants confirmed that particular chromosomes of H. vulgare were replaced by their H. bulbosum homoeologues. For most probes, heterozygosity between pairs of H. bulbosum chromosomes was recorded. A possible duplication involving H. bulbosum homoeologues of barley chromosomes 4 and 7 was observed. Although molecular and cytological methods for analyzing chromosomally engineered plants are complementary, molecular probes may uncover differences not discernible using light microscopy or isozyme analysis.Key words: Hordeum vulgare, Hordeum bulbosum, chromosome substitutions, restriction fragment length polymorphism.

Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare)

Genome ◽  
1991 ◽  
Vol 34 (3) ◽  
pp. 437-447 ◽  
Author(s):  
M. Heun ◽  
A. E. Kennedy ◽  
J. A. Anderson ◽  
N. L. V. Lapitan ◽  
M. E. Sorrells ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Cdna Clones ◽  
Hordeum Vulgare L ◽  
Chinese Spring Wheat ◽  
Restriction Fragment Length

A restriction fragment length polymorphism (RFLP) map for barley (Hordeum vulgare L.) has been developed on the basis of a population of anther culture derived, doubled haploids from the F1 of cultivars 'Proctor' × 'Nudinka'. Wheat genomic, oat cDNA, and barley cDNA clones provided the major source of the probes. Additional clones included genomic clones from Aegilops squarrosa L. and barley, two dehydrin genes, a barley promoter, and a wheat ribosomal gene. In total, 155 RFLPs and two known genes (hulless and Mla12 powdery mildew resistance) have been placed on the linkage map. Clones were assigned to chromosomes using disomic wheat–barley chromosome addition lines. Chromosome arm orientation was done using 'Chinese Spring' wheat nullisomic–tetrasomic and ditelosomic aneuploid stocks. Markers have been located on all chromosome arms except 6S, and the total map length is 1096 centimorgans (cM). Considerations involved in RFLP mapping, including choice and number of restriction endonucleases, disturbed segregation ratios, and uneven distribution of markers in the genome are discussed as well as future improvements and applications of this map.Key words: restriction fragment length polymorphism, mapping, Hordeum vulgare L., Mla12.

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