A cytogenetically based physical map of chromosome 1B in common wheat

Genome ◽  
1993 ◽  
Vol 36 (3) ◽  
pp. 548-554 ◽  
Author(s):  
R. S. Kota ◽  
K. S. Gill ◽  
B. S. Gill ◽  
T. R. Endo
Keyword(s):  
Genetic Markers ◽  
Common Wheat ◽  
Hexaploid Wheat ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Physical Map ◽  
Wheat Chromosome ◽  
Homozygous Deletion ◽  
Genetic Maps ◽  
Rflp Markers

We have constructed a cytogenetically based physical map of chromosome 1B in common wheat by utilizing a total of 18 homozygous deletion stocks. It was possible to divide chromosome 1B into 17 subregions. Nineteen genetic markers are physically mapped to nine subregions of chromosome 1B. Comparison of the cytological map of chromosome 1B with an RFLP-based genetic linkage map of Triticum tauschii revealed that the linear order of the genetic markers was maintained between chromosome 1B of hexaploid wheat and 1D of T. tauschii. Striking differences were observed between the physical and genetic maps in relation to the relative distances between the genetic markers. The genetic markers clustered in the middle of the genetic map were physically located in the distal regions of both arms of chromosome 1B. It is unclear whether the increased recombination in the distal regions of chromosome 1B is due to specific regions of increased recombination or a more broadly distributed increase in recombination in the distal regions of Triticeae chromosomes.Key words: common wheat, chromosome 1B, homozygous deletion lines, physical map, RFLP markers.

A reference cross DNA panel for zebrafish (Danio rerio) anchored with simple sequence length polymorphisms

Development ◽  
1996 ◽  
Vol 123 (1) ◽  
pp. 451-460 ◽  
Author(s):  
E.W. Knapik ◽  
A. Goodman ◽  
O.S. Atkinson ◽  
C.T. Roberts ◽  
M. Shiozawa ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Markers ◽  
Genetic Linkage ◽  
Positional Cloning ◽  
Genetic Linkage Map ◽  
Physical Map ◽  
Sequence Length ◽  
Mapping Tool ◽  
Length Polymorphisms ◽  
Simple Sequence

The ultimate informativeness of the zebrafish mutations described in this issue will rest in part on the ability to clone these genes. However, the genetic infrastructure required for the positional cloning in zebrafish is still in its infancy. Here we report a reference cross panel of DNA, consisting of 520 F2 progeny (1040 meioses) that has been anchored to a zebrafish genetic linkage map by 102 simple sequence length polymorphisms. This reference cross DNA provides: (1) a panel of DNA from the cross that was used to construct the genetic linkage map, upon which polymorphic gene(s) and genetic markers can be mapped; (2) a fine order mapping tool, with a maximum resolution of 0.1 cM; and (3) a foundation for the development of a physical map (an ordered array of clones each containing a known portion of the genome). This reference cross DNA will serve as a resource enabling investigators to relate genes or genetic markers directly to a single genetic linkage map and avoid the problem of integrating different maps with different genetic markers, as must be currently done when using randomly amplified polymorphic DNA markers, or as has occurred with human genetic linkage maps.

scholarly journals A Dense Genetic Linkage Map for Common Carp and Its Integration with a BAC-Based Physical Map

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e63928 ◽  
Author(s):  
Lan Zhao ◽  
Yan Zhang ◽  
Peifeng Ji ◽  
Xiaofeng Zhang ◽  
Zixia Zhao ◽  
...  
Keyword(s):  
Linkage Map ◽  
Common Carp ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Physical Map

scholarly journals A High-Density Integrated Genetic Linkage and Radiation Hybrid Map of the Laboratory Rat

1999 ◽  
Vol 9 (6) ◽  
pp. AP1-AP8 ◽  
Author(s):  
Robert G. Steen ◽  
Anne E. Kwitek-Black ◽  
Christopher Glenn ◽  
Jo Gullings-Handley ◽  
William Van Etten ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Web Sites ◽  
Radiation Hybrid ◽  
Genetic Linkage ◽  
Large Scale ◽  
Inbred Strains ◽  
Genetic Maps ◽  
Sequence Length ◽  
Link Type ◽  
Laboratory Rat

The laboratory rat (Rattus norvegicus) is a key animal model for biomedical research. However, the genetic infrastructure required for connecting phenotype and genotype in the rat is currently incomplete. Here, we report the construction and integration of two genomic maps: a dense genetic linkage map of the rat and the first radiation hybrid (RH) map of the rat. The genetic map was constructed in two F2 intercrosses (SHRSP × BN and FHH × ACI), containing a total of 4736 simple sequence length polymorphism (SSLP) markers. Allele sizes for 4328 of the genetic markers were characterized in 48 of the most commonly used inbred strains. The RH map is a lod ≥ 3 framework map, including 983 SSLPs, thereby allowing integration with markers on various genetic maps and with markers mapped on the RH panel. Together, the maps provide an integrated reference to >3000 genes and ESTs and >8500 genetic markers (5211 of our SSLPs and >3500 SSLPs developed by other groups). [Bihoreau et al. (1997); James and Tanigami, RHdb (http://www.ebi.ac.uk/RHdb/index.html); Wilder (http://www.nih.gov/niams/scientific/ratgbase); Serikawa et al. (1992); RATMAP server (http://ratmap.gen.gu.se)] RH maps (v. 2.0) have been posted on our web sites at http://goliath.ifrc.mcw.edu/LGR/index.htmlor http://curatools.curagen.com/ratmap. Both web sites provide an RH mapping server where investigators can localize their own RH vectors relative to this map. The raw data have been deposited in the RHdb database. Taken together, these maps provide the basic tools for rat genomics. The RH map provides the means to rapidly localize genetic markers, genes, and ESTs within the rat genome. These maps provide the basic tools for rat genomics. They will facilitate studies of multifactorial disease and functional genomics, allow construction of physical maps, and provide a scaffold for both directed and large-scale sequencing efforts and comparative genomics in this important experimental organism.

scholarly journals A Genetic Linkage Map of the House Musk Shrew, Suncus murinus, Constructed with PCR-Based and RFLP Markers

2008 ◽  
Vol 57 (2) ◽  
pp. 129-134
Author(s):  
Samuel ADJEI ◽  
Akira SATO ◽  
Takahiro NAGASE ◽  
Kazumi MATSUBARA ◽  
Yoichi MATSUDA ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Rflp Markers ◽  
Suncus Murinus ◽  
Musk Shrew

scholarly journals A genetic linkage map of the mouse using restriction landmark genomic scanning (RLGS).

Genetics ◽  
1994 ◽  
Vol 138 (4) ◽  
pp. 1207-1238 ◽  
Author(s):  
Y Hayashizaki ◽  
S Hirotsune ◽  
Y Okazaki ◽  
H Shibata ◽  
A Akasako ◽  
...  
Keyword(s):  
Genome Analysis ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Mouse Genome ◽  
Restriction Enzymes ◽  
Genetic Maps ◽  
Mus Spretus ◽  
Restriction Landmark Genomic Scanning ◽  
Two Dimensional Gel Electrophoresis ◽  
Probe Hybridization

Abstract We have developed a multiplex method of genome analysis, restriction landmark genomic scanning (RLGS) that has been used to construct genetic maps in mice. Restriction landmarks are end-labeled restriction fragments of genomic DNA that are separated by using high resolution, two-dimensional gel electrophoresis identifying as many as two thousand landmark loci in a single gel. Variation for several hundred of these loci has been identified between laboratory strains and between these strains and Mus spretus. The segregation of more than 1100 RLGS loci has been analyzed in recombinant inbred (RI) strains and in two separate interspecific genetic crosses. Genetic maps have been derived that link 1045 RLGS loci to reference loci on all of the autosomes and the X chromosome of the mouse genome. The RLGS method can be applied to genome analysis in many different organisms to identify genomic loci because it uses end-labeling of restriction landmarks rather than probe hybridization. Different combinations of restriction enzymes yield different sets of RLGS loci providing expanded power for genetic mapping.

Mapping the Genome of a Model Protochordate. I. A Low Resolution Genetic Map Encompassing the Fusion/Histocompatibility (Fu/HC) Locus of Botryllus schlosseri

Genetics ◽  
1998 ◽  
Vol 149 (1) ◽  
pp. 277-287
Author(s):  
Anthony W De Tomaso ◽  
Yasunori Saito ◽  
Katharine J Ishizuka ◽  
Karla J Palmeri ◽  
Irving L Weissman
Keyword(s):  
Genetic Markers ◽  
Peripheral Blood ◽  
Genetic Linkage ◽  
Bulk Segregant Analysis ◽  
Inflammatory Process ◽  
Genetic Linkage Map ◽  
Molecular Genetic ◽  
Dna Polymorphisms ◽  
Botryllus Schlosseri ◽  
Genomic Approach

Abstract The colonial protochordate, Botryllus schlosseri, undergoes a genetically defined, natural transplantation reaction when the edges of two growing colonies interact. Peripheral blood vessels of each colony touch and will either fuse together to form a common vasculature between the colonies, or reject each other in an active blood-based inflammatory process in which the interacting vessels are cut off and the two colonies no longer interact. Previous studies have demonstrated that allorecognition in Botryllus is principally controlled by a single Mendelian locus named the fusion/histocompatibility (Fu/HC) locus, with multiple codominantly expressed alleles. However, identification and cloning of this locus has been difficult. We are taking a genomic approach in isolating this locus by creating a detailed genetic linkage map of the 725 Mbp Botryllus genome using DNA polymorphisms (primarily identified as AFLPs) as molecular genetic markers. DNA polymorphisms are identified in inbred laboratory strains of Fu/HC defined Botryllus, and their segregation and linkage is analyzed in a series of defined crosses. Using bulk segregant analysis, we have focused our mapping efforts on the Fu/HC region of the genome, and have generated an initial map which delineates the Fu/HC locus to a 5.5 cM region.

Construction of a genetic linkage map for Saccharum officinarum incorporating both simplex and duplex markers to increase genome coverage

Genome ◽  
2007 ◽  
Vol 50 (8) ◽  
pp. 742-756 ◽  
Author(s):  
K.S. Aitken ◽  
P.A. Jackson ◽  
C.L. McIntyre
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Saccharum Officinarum ◽  
Basic Chromosome Number ◽  
Genetic Maps ◽  
Sugarcane Cultivar ◽  
Repulsion Phase ◽  
Preferential Chromosome Pairing ◽  
Segregating Population

Saccharum officinarum L. is an octoploid with 80 chromosomes and a basic chromosome number of x = 10. It has high stem sucrose and contributes 80% of the chromosomes to the interspecific sugarcane cultivars that are grown commercially for sucrose. A genetic linkage map was developed for S. officinarum (clone IJ76-514) using a segregating population generated from a cross between Q165 (a commercial sugarcane cultivar) and IJ76-514. In total, 40 AFLP and 72 SSR primer pairs were screened across the population, revealing 595 polymorphic bands inherited from IJ76-514. These 595 markers displayed a frequency distribution different from all other sugarcane genetic maps produced, with only 40% being simplex markers (segregated 1:1). Of these 240 simplex markers, 178 were distributed on 47 linkage groups (LGs) and 62 remained unlinked. With the addition of 234 duplex markers and 80 biparental simplex markers (segregating 3:1), 534 markers formed 123 LGs. Using the multi-allelic SSR markers, repulsion phase linkage, and alignment with the Q165 linkage map, 105 of the 123 LGs could be grouped into 10 homology groups (HGs). These 10 HGs were further assigned to the 8 HGs observed in cultivated sugarcane and S. spontaneum . Analysis of repulsion phase linkage indicated that IJ76-514 is neither a complete autopolyploid nor an allopolyploid. Detection of 28 repulsion linkages that occurred between 6 pairs of LGs located in 4 HGs suggested the occurrence of limited preferential chromosome pairing in this species.

Genetic linkage map in sour cherry using RFLP markers

1998 ◽  
Vol 97 (8) ◽  
pp. 1217-1224 ◽  
Author(s):  
D. Wang ◽  
R. Karle ◽  
T. S. Brettin ◽  
A. F. Iezzoni
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Sour Cherry ◽  
Rflp Markers

Advances in genetic mapping of the sorghum genome

2006 ◽  
Vol 3 (3) ◽  
pp. 155-161
Author(s):  
Yi Zhi-Ben ◽  
Sun Yi ◽  
Liang Xiao-Hong ◽  
Zhao Wei-Jun ◽  
Yan Min ◽  
...  
Keyword(s):  
Genetic Linkage ◽  
Physical Map ◽  
Organizer Region ◽  
Molecular Genetic ◽  
Nucleolus Organizer Region ◽  
Nucleolus Organizer ◽  
Genetic Maps ◽  
Linkage Groups ◽  
Sorghum Genome ◽  
Molecular Cytogenetic

AbstractThe construction of the sorghum (Sorghum bicolor L. Moench) molecular genetic linkage map started in the early 1990s. Molecular genetic maps with a high density of markers covering almost the entire sorghum genome have been completed and integration of a sorghum genetic and physical map is under way. The correlation between genetic linkage groups and relevant chromosomes was established and the locations of the important structures of chromosomes, such as centromeres, long and short arms, nucleolus organizer region (NOR), etc., have been identified on the linkage groups. Molecular cytogenetic mapping of each chromosome has been advanced substantially. With continuing progress in the field, sequencing of the full sorghum genome and study of sorghum functional genomics will be initiated soon.

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