Genetic linkage map in sour cherry using RFLP markers

1998 ◽  
Vol 97 (8) ◽  
pp. 1217-1224 ◽  
Author(s):  
D. Wang ◽  
R. Karle ◽  
T. S. Brettin ◽  
A. F. Iezzoni
2008 ◽  
Vol 57 (2) ◽  
pp. 129-134
Author(s):  
Samuel ADJEI ◽  
Akira SATO ◽  
Takahiro NAGASE ◽  
Kazumi MATSUBARA ◽  
Yoichi MATSUDA ◽  
...  

Genetics ◽  
1992 ◽  
Vol 132 (4) ◽  
pp. 1003-1015 ◽  
Author(s):  
L D Sibley ◽  
A J LeBlanc ◽  
E R Pfefferkorn ◽  
J C Boothroyd

Abstract We have constructed a genetic linkage map for the parasitic protozoan, Toxoplasma gondii, using randomly selected low copy number DNA markers that define restriction fragment length polymorphisms (RFLPs). The inheritance patterns of 64 RFLP markers and two phenotypic markers were analyzed among 19 recombinant haploid progeny selected from two parallel genetic crosses between PLK and CEP strains. In these first successful interstrain crosses, these RFLP markers segregated into 11 distinct genetic linkage groups that showed close correlation with physical linkage groups previously defined by molecular karyotype. Separate linkage maps, constructed for each of the 11 chromosomes, indicated recombination frequencies range from approximately 100 to 300 kb per centimorgan. Preliminary linkage assignments were made for the loci regulating sinefungin resistance (snf-1) on chromosome IX and adenine arabinoside (ara-1) on chromosome V by linkage to RFLP markers. Despite random segregation of separate chromosomes, the majority of chromosomes failed to demonstrate internal recombination events and in 3/19 recombinant progeny no intramolecular recombination events were detected. The relatively low rate of intrachromosomal recombination predicts that tight linkage for unknown genes can be established with a relatively small set of markers. This genetic linkage map should prove useful in mapping genes that regulate drug resistance and other biological phenotypes in this important opportunistic pathogen.


HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 403C-403
Author(s):  
Anne M. Gillen ◽  
Fredrick A. Bliss

Peach rootstock breeding may be accelerated by utilization of molecular markers linked to the root-knot nematode resistance locus (Mi) to screen segregating populations. A genetic linkage map was constructed using RFLP markers in an F2 population (PMP2) that is segregating for this locus. PMP2 is derived from a controlled cross of the relatively diverse peach rootstocks Harrow Blood (susceptible) and Okinawa (homozygous resistant). Bulked Segregant Analysis was applied using RAPD markers. A single small (227 base pairs) RAPD marker was found to be linked to the dominant resistant allele of Mi at a distance of 10 cM. This new marker joined the Mi locus to the RFLP linkage map and showed that two dominant RFLP markers are located between the RAPD marker and Mi. RFLPS are expensive, time-consuming and RAPD markers are unreliable, and therefore both are unsuitable for screening breeding populations. We attempted to convert the RAPD marker to a more breeder-friendly CAPS marker. The converted CAP marker was dominant. Attempts to convert the CAP marker to a co-dominant marker were not successful. The utility of the CAP marker was tested in an open pollinated F2 population derived from the F1 parent of PMP2 and in several rootstocks. The genetic linkage map was compared to other Prunus maps. The PMP2 linkage group containing the Mi locus can be related to the peach × almond linkage group which contains the phosphoglucomutase Pgm-1 locus.


2009 ◽  
Vol 31 (6) ◽  
pp. 629-637 ◽  
Author(s):  
Wei-Dong LIU ◽  
Xiang-Bo BAO ◽  
Wen-Tao SONG ◽  
Zun-Chun ZHOU ◽  
Chong-Bo HE ◽  
...  

2016 ◽  
Vol 42 (2) ◽  
pp. 159 ◽  
Author(s):  
Jian-Bin GUO ◽  
Li HUANG ◽  
Liang-Qiang CHENG ◽  
Wei-Gang CHEN ◽  
Xiao-Ping REN ◽  
...  

2013 ◽  
Vol 19 (6) ◽  
pp. 930-938 ◽  
Author(s):  
Renyi PANG ◽  
Wentao SONG ◽  
Fengtao GAO ◽  
Xiaolin LIAO ◽  
Yongwei ZHAO ◽  
...  

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