C-banding polymorphism and linkage of nonhomoeologous RFLP loci in the D genome progenitor of wheat

Genome ◽  
1993 ◽  
Vol 36 (2) ◽  
pp. 235-243 ◽  
Author(s):  
U. Hohmann ◽  
E. S. Lagudah
Keyword(s):  
Linkage Mapping ◽  
High Frequency ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Rflp Markers ◽  
Homoeologous Group ◽  
D Genome ◽  
C Banding ◽  
Map Construction ◽  
Group 1

Chromosomes from four different accessions of Triticum tauschii, used as parents in generating F2 populations for RFLP genetic linkage map construction, were analyzed by C-banding. The accessions consist of the varietal taxa strangulata (AUS 21929) and meyeri (AUS 18911), and two genotypes of var. typica (AUS 18902 and CPI 110730 from Iran and Afghanistan, respectively). Chromosomes 1D and 7D of T. tauschii var. typica AUS 18902 are involved in a reciprocal interchange forming translocated chromosomes, T1DS.7DL and T7DS.1DL, with tbe breakpoints being located within the centrometric region. The formation of quadrivalent configuration in F1 hybrids provided further confirmation of the reciprocal translocation. Genetic linkage mapping of additional RFLP markers located on homoeologous group 1 and 7 chromosomes showed consistent linkage to a composite group of proximal markers on chromosomes 1D and 7D of a previously published map derived from the F2 progeny of AUS 18902 × AUS 18911. A high frequency of RFLP genotypes transmitted by the translocation parent was prevalent in the proximal regions of chromosomes 1D and 7D. Genotypic frequencies expected of the nontranslocated parental RFLP markers was evident only in the distal regions of these chromosomes.Key words: C-banding, genetic map, translocation heterozygote, RFLP, linkage.

scholarly journals Genetic Linkage of Randomly Amplified Polymorphic DNA (RAPD) Markers in Sweetpotato

1997 ◽  
Vol 122 (1) ◽  
pp. 79-82 ◽  
Author(s):  
Paul. G. Thompson ◽  
Liang L. Hong ◽  
Kittipat Ukoskit ◽  
Zhiqiang Zhu
Keyword(s):  
Linkage Map ◽  
Linkage Mapping ◽  
Genetic Linkage ◽  
Ipomoea Batatas ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Rapd Marker ◽  
Randomly Amplified Polymorphic Dna ◽  
Genetic Linkage Mapping ◽  
Map Construction

RAPD marker analyses were completed on parents and progeny of two sweetpotato [Ipomoea batatas (L.) Lam.] crosses to determine the feasibility of genetic linkage map construction. A total of 100 primers was tested and 96 produced amplified genomic DNA fragments. The average number of polymorphisms per primer was 0.69. A total of 134 polyphorphic markers was observed and 74 (60%) segregated 1 band present : 1 band absent as needed for use in genetic linkage mapping of polyploids. The 60% of RAPD markers that segregated 1:1 shows that genetic linkage mapping of the hexaploid sweetpotato by RAPD marker analysis is feasible. Linkage was determined for all markers that segregated 1:1 and five pairs of linked markers were found. These were the first linked molecular markers found in sweetpotato and they show that construction of a genetic linkage map is feasible. A genetic linkage map will be a valuable tool to assist in genetic improvements.

scholarly journals 671 PB 145 GENETIC LINKAGE MAPPING IN PEACH

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 529a-529
Author(s):  
S. Rajapakse ◽  
L. E. Belthoff ◽  
R. E. Ballard ◽  
R. Scorza ◽  
W.V. Baird ◽  
...  
Keyword(s):  
Linkage Mapping ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Average Distance ◽  
Nuclear Genome ◽  
Rflp Analysis ◽  
Rflp Markers ◽  
Flesh Color ◽  
Self Fertilization ◽  
Rflp Rapd

We have constructed a genetic linkage map of peach consisting of RFLP, RAPD, and morphological markers, based on 78 F2 individuals derived from the self-fertilization of four F1 individuals originating from a cross between `New Jersey Pillar' and KV 77119. This progeny set was chosen because parental genotypes exhibit variation in canopy shape, fruit flesh color, and flower petal color, size, and number. The segregation of 81 markers comprised of RFLP, RAPD and morphological loci was analyzed. Low copy genomic and cDNA probes were used in the RFLP analysis. The current genetic map for the WV family contains 57 markers assigned to 9 linkage groups, which cover 520 cM of the peach nuclear genome. The average distance between two adjacent markers was 9 cM. Linkage was detected between Pillar (Pi) and double flowers (Dl). RFLP markers loosely linked to Pi, flesh color (Y), and white flower (W) loci were found. Twenty-four markers remain unassigned.

Detection of polymorphic simple-sequence repeat markers that show linkage to a novel sugarcane brown rust disease resistance gene in resistant and susceptible genetic pools

2020 ◽  
pp. 1-7
Author(s):  
Jie Li ◽  
Xiao-Yan Wang ◽  
Hong-Li Shan ◽  
Rong-Yue Zhang ◽  
Chan-Mi Wang ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Brown Rust ◽  
Cross Combination ◽  
Map Construction ◽  
Parental Lines ◽  
Genetic Pools ◽  
Simple Sequence ◽  
Rust Resistance Genes

Abstract Sugarcane brown rust, caused by Puccinia melanocephala, is one of the main diseases of sugarcane in China. The identification and discovery of new resistance genes have important theoretical and practical significance for preventing outbreaks of brown rust and ensuring the sustainable production of sugarcane. To screen for polymorphic simple-sequence repeat (SSR) molecular markers for localization of brown rust resistance genes, we used two populations that are suitable for genetic linkage map construction and mapping of new resistance genes to construct resistant and susceptible genetic pools. We then screened 449 pairs of primers to identify polymorphic SSR markers in the parental lines and the resistant/susceptible genetic pools. The results showed that 25 pairs of primers directed amplification of polymorphic DNA fragments between the parents of the cross combination ‘Yuetang 03-393’ × ‘ROC 24’, and 16 pairs of primers amplified polymorphic fragments between the parents of the cross combination ‘Liucheng 03-1137’ × ‘Dezhe 93-88’. Four pairs of primers (SMC236CG, SCESSR0928, SCESSR0636 and SCESSR2551) amplified polymorphic DNA fragments between the parental lines and the resistant/susceptible genetic pools in ‘Yuetang 03-393’ × ‘ROC 24’. The results of this study will establish a solid foundation for the mapping of new brown rust resistance genes, genetic linkage map construction and the development of closely-associated molecular markers in sugarcane.

scholarly journals A Genetic Linkage Map of the House Musk Shrew, Suncus murinus, Constructed with PCR-Based and RFLP Markers

2008 ◽  
Vol 57 (2) ◽  
pp. 129-134
Author(s):  
Samuel ADJEI ◽  
Akira SATO ◽  
Takahiro NAGASE ◽  
Kazumi MATSUBARA ◽  
Yoichi MATSUDA ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Rflp Markers ◽  
Suncus Murinus ◽  
Musk Shrew

A cytogenetically based physical map of chromosome 1B in common wheat

Genome ◽  
1993 ◽  
Vol 36 (3) ◽  
pp. 548-554 ◽  
Author(s):  
R. S. Kota ◽  
K. S. Gill ◽  
B. S. Gill ◽  
T. R. Endo
Keyword(s):  
Genetic Markers ◽  
Common Wheat ◽  
Hexaploid Wheat ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Physical Map ◽  
Wheat Chromosome ◽  
Homozygous Deletion ◽  
Genetic Maps ◽  
Rflp Markers

We have constructed a cytogenetically based physical map of chromosome 1B in common wheat by utilizing a total of 18 homozygous deletion stocks. It was possible to divide chromosome 1B into 17 subregions. Nineteen genetic markers are physically mapped to nine subregions of chromosome 1B. Comparison of the cytological map of chromosome 1B with an RFLP-based genetic linkage map of Triticum tauschii revealed that the linear order of the genetic markers was maintained between chromosome 1B of hexaploid wheat and 1D of T. tauschii. Striking differences were observed between the physical and genetic maps in relation to the relative distances between the genetic markers. The genetic markers clustered in the middle of the genetic map were physically located in the distal regions of both arms of chromosome 1B. It is unclear whether the increased recombination in the distal regions of chromosome 1B is due to specific regions of increased recombination or a more broadly distributed increase in recombination in the distal regions of Triticeae chromosomes.Key words: common wheat, chromosome 1B, homozygous deletion lines, physical map, RFLP markers.

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