Restriction fragment analysis of ribosomal and hordein genes in eastern Canadian two-rowed barleys

Genome ◽  
1991 ◽  
Vol 34 (2) ◽  
pp. 298-302 ◽  
Author(s):  
Stephen J. Molnar ◽  
Adèle McKay
Keyword(s):  
Restriction Fragment ◽  
Ribosomal Dna ◽  
Fragment Length ◽  
Seed Storage Protein ◽  
Restriction Fragment Length Polymorphisms ◽  
Fragment Analysis ◽  
Restriction Fragment Analysis ◽  
Barley Cultivars ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

Restriction fragment length polymorphisms (RFLPs) have been advocated as useful genetic markers for a variety of genetic and plant breeding applications. Among barley cultivars, RFLPs have been reported in three well-characterized multigene families: at the hordein seed storage protein loci, at the 18S + 5.8S + 26S ribosomal DNA loci, and for 5S ribosomal DNA. The present study sampled RFLPs in these three gene families in the five major eastern Canadian two-rowed barley cultivars. The target sequences constitute at least six loci distributed over at least three chromosomes but represent a very small percentage of the total genome. The five cultivars studied share common alleles at the 18S + 5.8S + 26S ribosomal loci Rrn 1 and Rrn 2 and at the 5S ribosomal loci. This may reflect a fairly narrow genetic base for these cultivars. Moderate amounts of polymorphism were detected at the Hor 1 and Hor 2 loci, each of which comprises a small multigene family. Restriction fragment length polymorphism complemented protein polymorphism at the hordein loci, arguing for their joint use for cultivar identification or similar purposes.Key words: barley, restriction fragment analysis, restriction fragment length, polymorphisms, hordeins, ribosomal DNA.

Studies on keratinophilic fungi. X. Arthrographis alba sp.nov.

1996 ◽  
Vol 42 (12) ◽  
pp. 1185-1189 ◽  
Author(s):  
Josepa Gené ◽  
Josep Guarro ◽  
José Manuel Guillamón ◽  
Krzysztof Ulfig
Keyword(s):  
Restriction Fragment ◽  
Ribosomal Dna ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Keratinophilic Fungi ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

In our studies on keratinophilic fungi in Spain, an arthroconidial anamorphic species has been recovered and is herein described as new. Arthrographis alba sp.nov. is characterized by having arthroconidia borne from laterally or pseudodichotomously branched conidiophores, white colonies, and nil or restricted growth at 37 °C. Analysis of restriction fragment length polymorphisms of ribosomal DNA reveals differences with Arthrographis kalrae, a morphologically similar species.Key words: Arthrographis alba, Hyphomycetes, rDNA.

Restriction fragment length polymorphisms in polymerase chain reaction amplified ribosomal DNAs of three Trichogramma (Hymenoptera: Trichogrammatidae) species

Genome ◽  
1995 ◽  
Vol 38 (3) ◽  
pp. 419-425 ◽  
Author(s):  
Navtej Pal Sappal ◽  
Robert S. Jeng ◽  
Martin Hubbes ◽  
Fuhua Liu
Keyword(s):  
Restriction Fragment ◽  
Ribosomal Dna ◽  
Restriction Enzyme ◽  
Fragment Length ◽  
Its Region ◽  
Restriction Fragment Length Polymorphisms ◽  
Length Variation ◽  
Length Polymorphisms ◽  
Ribosomal Dnas ◽  
Restriction Fragment Length

Restriction fragment length polymorphisms from PCR amplified ribosomal DNAs of three Trichogramma species, T. minutum, T. brassicae, and T. near sibiricum, were studied. Length variation in the internal transcribed spacer (ITS) region was observed. The ITS region of T. brassicae is about 1350 base pairs (bp) in length and those of T. minutum and T. near sibiricum are 1300 bp. These three species also differ in the size of their ITS1 and ITS2 regions. Restriction enzyme digestions of these regions showed unique banding patterns for each species. The amplified 18S region of ribosomal DNA is about 1800 bp in length and showed no length variation between the three species of Trichogramma. Restriction enzyme digestion of this region by BamHI differentiated T. brassicae from the other two species. Restriction site maps of the ITS and 18S regions were constructed for each species. The amplified 28S region is about 1700 bp for these three species. Restriction of this region by RsaI and SacII differentiates these three species. The reported results indicate that these species of Trichogramma can be clearly differentiated from one another by nuclear ribosomal DNA markers.Key words: rDNA, Trichogramma, PCR.

Polymorphisms in the nuclear DNA of Achlya species: some taxonomic implications

1989 ◽  
Vol 35 (12) ◽  
pp. 1146-1155 ◽  
Author(s):  
J. Stephen Horton ◽  
Paul A. Horgen
Keyword(s):  
Southern Blot ◽  
Restriction Fragment ◽  
Ribosomal Dna ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Dna Polymorphisms ◽  
Rrna Gene ◽  
Cdna Clones ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

Within the genus Achyla, which belongs to the class of fungi known as the Oomycetes, taxonomic judgments have traditionally been made using a variety of sexual criteria. We have used restriction fragment length polymorphisms as a new taxonomic character to examine intra- and inter-specific variation within this genus. Using a cDNA clone coding for the Achlya 18S rRNA gene as a hybridization probe, a 10-kb fragment of ribosomal DNA (rDNA) from Achlya ambisexualis strain E87 was cloned and then mapped for selected restriction enzyme sites. In Southern blot hybridizations, both this rDNA fragment and cloned 18S cDNAs revealed differences in the rDNA organization of A. ambisexualis E87 (male) and a female isolate of A. ambisexualis strain 734. No differences in the rDNAs were detected between the two heterothallic isolates A. ambisexualis E87 and A. bisexualis 65-1. Southern blot hybridizations suggested that two different rDNA organizations may exist within the genome of the homothallic strain A. heterosexualis B14. cDNA clones coding for two different hormonally regulated genes revealed the same relationships between the four isolates studied as those determined with rDNA probes. Two homothallic Achlya strains recently isolated from nature were found to have additional DNA polymorphisms not detected in the laboratory strains. Phenetic analysis distinguished the same similarities that were evident upon inspection of the hybridization data. Taken together, these data suggest different relationships between the isolates examined than do the previous taxonomic criteria by which species have been delimited within this genus.Key words: Achlya; restriction fragment length polymorphisms; ribosomal DNA; taxonomy.

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