Relationships Among Fusarium SPP. Estimated by Comparing Restriction Fragment Length Polymorphisms in Polymerase Chain Reaction-Amplified Ribosomal DNA

1997 ◽  
Vol 25 (3) ◽  
pp. 577-578
Author(s):  
G. L. Bateman ◽  
H. Kwasna ◽  
E. Ward
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Fragment ◽  
Ribosomal Dna ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Fusarium Spp ◽  
Chain Reaction ◽  
Length Polymorphisms ◽  
Polymerase Chain ◽  
Restriction Fragment Length

Polymerase chain reaction - restriction fragment length polymorphisms for assessing and increasing biodiversity ofFrankiaculture collections

1999 ◽  
Vol 77 (9) ◽  
pp. 1261-1269 ◽  
Author(s):  
Erica Lumini ◽  
Marco Bosco
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Intergenic Spacer ◽  
Chain Reaction ◽  
Culture Collections ◽  
Length Polymorphisms ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
Restriction Fragment Length

During the last few years, some Frankia culture collections that maintained a large number of unidentified and uncharacterized Frankia strains were closed because of funding shortages. To reduce the costs of maintenance, we evaluated the biodiversity of half of the Frankia strains from our collection, by polymerase chain reaction - restriction fragment length polymorphisms (PCR-RFLPs) of nifD-nifK intergenic spacer and 16S-23S rDNA intergenic spacer regions. In this way we were able to reduce the number of strains without reducing the biodiversity of the whole collection. In general the nifD-nifK target proved to be more polymorphic than the rrn target. From 51 isolates of Elaeagnus frankiae, PCR-RFLP results allowed us to detect 13 identical strains, and to predict that the genomic species P8 of Akimov and Dobritsa (1992) very likely agrees with genomic species 5 of Fernandez et al. (1989). Moreover, we revealed genomic groups not yet described, as well as intraspecific variability. For Alnus frankiae, the polymorphisms shown by both the nif and the rrn PCR-RFLPs revealed three host plant species-specific subgroups inside Frankia alni. An expandable data base was created to serve as reference for future biodiversity evaluations on both culture collections and unisolated Frankia populations. It will be accessible by Internet at the International Frankia Website (http://www.unifi.it/unifi/distam/frankia/international.html).Key words: Frankia, PCR-RFLP, nifD-nifK intergenic spacer, rrn 16S-23S intergenic spacer, biodiversity, culture collections.

scholarly journals Comparison of DNA Profiling between Fishes and Pork Meat using Polymerase Chain Reaction-Restriction Fragment Length Polymorphisms (PCR-RFLP) Analysis

2018 ◽  
Vol 47 (07) ◽  
pp. 1535-1540
Author(s):  
Safiyyah Shahimi ◽  
Sahilah Abd. Mutalib ◽  
Wan Sakeenah Wan Nazri ◽  
Aminah Abdullah ◽  
Norrakiah Abdullah Sani
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Rflp Analysis ◽  
Pork Meat ◽  
Chain Reaction ◽  
Length Polymorphisms ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
Restriction Fragment Length
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