Synaptonemal complex formation in male scorpions exhibiting achiasmate meiosis and structural heterozygosity

Genome ◽  
1990 ◽  
Vol 33 (6) ◽  
pp. 914-926 ◽  
Author(s):  
Catherine M. Shanahan ◽  
David L. Hayman
Keyword(s):  
Complex Formation ◽  
Synaptonemal Complex ◽  
Meiotic Chromosome ◽  
Male Meiosis ◽  
Late Prophase ◽  
Maintenance System ◽  
Structural Heterozygosity ◽  
Synaptonemal Complex Formation ◽  
And Inversion ◽  
Metaphase I

Synaptonemal complex (SC) formation was studied in testicular material from individuals of a number of species from two families of Australian scorpions; Buthidae and Scorpionidae. These scorpions exhibit unusual cytogenetic features including achiasmate male meiosis, interchange heterozygosity, and centromeric fusion–fission and inversion heterozygosity. The synaptic behaviour of chromosomes involved in these rearrangements was studied from zygotene to metaphase I, using both meiotic chromosome preparations and techniques for examination of the SCs. Multivalent associations present during the achiasmate meiosis of both buthid and scorpionid scorpions are retained from prophase to metaphase I, unlike those present in polyploid achiasmate Bombyx females. Further evidence suggests that synaptic adjustment does not occur generally in achiasmate scorpionid inversion heterozygotes. However, for some inversions, pairing is seen to become more heterosynaptic from late prophase to metaphase I and this may be related to the pairing maintenance system during achiasmate meiosis in these specialized organisms.Key words: synaptonemal complex, achiasmate meiosis, heterozygosity, interchange, inversion.

Meiosis in triploid Lolium. I. Synaptonemal complex formation and chromosome configurations at metaphase I in aneuploid autotriploid L. multiflorum

Genome ◽  
1994 ◽  
Vol 37 (2) ◽  
pp. 181-189 ◽  
Author(s):  
Huw M. Thomas ◽  
Barry J. Thomas
Keyword(s):  
Complex Formation ◽  
Synaptonemal Complex ◽  
Lolium Multiflorum ◽  
Synaptonemal Complexes ◽  
Pollen Mother Cells ◽  
Partner Exchange ◽  
Synaptonemal Complex Formation ◽  
Mother Cells ◽  
Axial Element ◽  
Metaphase I

A spreading technique for synaptonemal complexes (SCs) was applied to pollen mother cells of two aneuploid genotypes of autotriploid Lolium multiflorum (2n = 3x + 1 = 22). In the earliest nuclei analyzed the axial elements are in six groups of 3 and one group of 4. Most groups have formed multivalents with from one to five pairing partner exchanges, but there are also groups that have formed bivalents and univalents. Some axial elements have formed triple associations, in one case for the length of the trivalent. Unsynapsed axial elements remain aligned with their homologous SCs into pachytene, but this alignment is abolished as these axes pair heterologously among themselves until the entire axial element complement is synapsed. At metaphase I most chromosomes are associated as trivalents and quadrivalents.Key words: Lolium, triploid, pairing partner exchange, chiasma, multivalent.

Synaptonemal complex formation and metaphase I configuration patterns in a translocation heterozygote of rye (Secale cereale)

Genome ◽  
1989 ◽  
Vol 32 (1) ◽  
pp. 72-81 ◽  
Author(s):  
J. H. de Jong ◽  
J. van Eden ◽  
J. Sybenga
Keyword(s):  
Complex Formation ◽  
Synaptonemal Complex ◽  
Secale Cereale ◽  
Chiasma Formation ◽  
Terminal Segment ◽  
Pachytene Nucleus ◽  
Synaptonemal Complexes ◽  
Synaptonemal Complex Formation ◽  
Chromosome 1R ◽  
Metaphase I

Four rye plants heterozygous for translocation 248, involving chromosomes 1R and 6R, were used for a comparative study of synaptonemal complex formation at midprophase I and chromosome configurations at metaphase I. Synaptonemal complexes were obtained with a cell-spreading technique and studied with electron microscopy. The total length of the synaptonemal complexes in the 28 analyzable pachytene nuclei varied considerably, both within and among plants. The variation of synaptonemal complex lengths of the bivalents in a nucleus was partly stage dependent; i.e., it was greater at early than at late pachytene. In all but one pachytene nucleus, pairing in the quadrivalent was regular, and the four pairing arms were usually easy to identify. Most noticeable was the variation of pairing saturation at the breakpoint of the quadrivalent. Pairing in the breakpoint region was delayed with respect to the pairing in the bivalents. Variation in the arm lengths of the quadrivalent was the result of incomplete and nonhomologous pairing at the breakpoint as well as differential contraction rates among chromosome segments. It was shown that the completion of delayed pairing throughout pachytene is mainly long-arm pairing. The actual breakpoint was therefore not in the middle of the unpaired segments, but more distal. The analysis of metaphase I nuclei revealed that chiasma frequency in this material was higher than in similar material used in former studies. When one of the translocation segments lacked a chiasma, this was in most cases the short translocated segment 1RS, the terminal segment of the satellite of chromosome 1R. Positive chiasma interference was demonstrated between the interstitial and exchanged segment in 1RS. This agreed with the observation of a negative correlation in extent of pairing between these two segments. Other interference phenomena, which have been described for this translocation in other material, remained undetected because of lack of variation in chiasma formation owing to high chiasma frequency.Key words: Secale cereale, meiosis, translocation, synaptonemal complex, metaphase I.

Synaptic abnormalities in spread nuclei of Secale. I. Inbred lines

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 764-771 ◽  
Author(s):  
M. Martínez ◽  
C. Cuadrado ◽  
C. Romero
Keyword(s):  
Complex Formation ◽  
Synaptonemal Complex ◽  
Secale Cereale ◽  
Inbred Lines ◽  
Meiotic Behaviour ◽  
Synaptonemal Complex Formation ◽  
Metaphase I

The meiotic behaviour at metaphase I of two inbred lines of Secale cereale showed a decrease in the frequency of bound arms and the presence of univalents. The study of synaptonemal complex formation revealed that these inbred lines had asynapsis and many nuclei did not complete the pairing process. Synaptic abnormalities such as fold-back loops, short pairing partner switches, interstitial nonhomologous pairing regions, and asynchrony between bivalents were associated and correlated with interlocking. It is suggested that the asynapsis is due to a failure in the interlocking repair. All these abnormalities could affect the formation and maintenance of chiasmata and so could explain the metaphase I behaviour of these inbred lines and the differences between them.Key words: inbred lines, Secale cereale, synaptonemal complex, spreading, synaptic abnormalities.

Comparison between synaptonemal complexes at pachytene and chromosome association at metaphase I in heterozygotes for a "nonreciprocal" translocation of rye

Genome ◽  
1989 ◽  
Vol 32 (6) ◽  
pp. 983-991 ◽  
Author(s):  
T. Naranjo ◽  
A. Roca ◽  
P. G. Goicoechea ◽  
J. H. de Jong ◽  
W. D. Smilde
Keyword(s):  
Complex Formation ◽  
Synaptonemal Complex ◽  
Secale Cereale ◽  
Chiasma Formation ◽  
Chromosome Association ◽  
Simultaneous Occurrence ◽  
Negative Interference ◽  
Secale Cereale L ◽  
Synaptonemal Complex Formation ◽  
Metaphase I

A comparative analysis of synaptonemal complex formation at pachytene and chromosome association at metaphase I was carried out in heterozygotes for translocation T242W (2R/6R) of rye (Secale cereale L.). Synaptonemal complex formation supported earlier light microscopic observations that one exchanged segment of this translocation was very small and restricted to the telomere or had been lost. Negative interference between the interstitial segments with respect to chiasma formation was detected at metaphase I. This interference was apparently the result of the simultaneous occurrence of either asynapsis or nonhomologous pairing around the translocation point at pachytene. Negative interference detected across the centromere of 6R was attributed to nonhomologous pairing. The presence of an intercalary C-band in the interstitial segment 2RLi or in the 1RS arm had no apparent influence on synaptonemal complex formation. Unmatched ends of synaptonemal complex 1R and of the multivalent were in most cases associated with heterozygosity for the telomeric C-heterochromatin.Key words: Secale cereale, translocation, synapsis, interference, C-banding.

scholarly journals Hormad1 Is a Germ Cell-Specific HORMA Containing Protein Required for Synaptonemal Complex Formation in Male Meiosis.

2009 ◽  
Vol 81 (Suppl_1) ◽  
pp. 172-172
Author(s):  
Yonghyun Shin ◽  
Malgorzata Kloc ◽  
Youngsok Choi ◽  
Marvin L. Meistrich ◽  
Aleksandar Rajkovic
Keyword(s):  
Complex Formation ◽  
Germ Cell ◽  
Synaptonemal Complex ◽  
Male Meiosis ◽  
Synaptonemal Complex Formation

Synaptonemal complex proteins: occurrence, epitope mapping and chromosome disjunction

1994 ◽  
Vol 107 (10) ◽  
pp. 2749-2760 ◽  
Author(s):  
M.J. Dobson ◽  
R.E. Pearlman ◽  
A. Karaiskakis ◽  
B. Spyropoulos ◽  
P.B. Moens
Keyword(s):  
Synaptonemal Complex ◽  
Fusion Proteins ◽  
Epitope Mapping ◽  
Core Protein ◽  
Binding Capacity ◽  
Polyclonal Antibodies ◽  
Meiotic Chromosome ◽  
Immunogold Labelling ◽  
Chromosome Disjunction ◽  
Metaphase I

We have used polyclonal antibodies against fusion proteins produced from cDNA fragments of a meiotic chromosome core protein, Cor1, and a protein present only in the synapsed portions of the cores, Syn1, to detect the occurrence and the locations of these proteins in rodent meiotic prophase chromosomes. The 234 amino acid Cor1 protein is present in early unpaired cores, in the lateral domains of the synaptonemal complex and in the chromosome cores when they separate at diplotene. A novel observation showed the presence of Cor1 axial to the metaphase I chromosomes and substantial amounts of Cor1 in association with pairs of sister centromeres. The centromere-associated Cor1 protein becomes dissociated from the centromeres at anaphase II and it is not found in mitotic metaphase centromeres. The extended presence of Cor1 suggests that it may have a role in chromosome disjunction by fastening chiasmata at metaphase I and by joining sister kinetochores, which ensures co-segregation at anaphase I. Two-colour immunofluorescence of Cor1 and Syn1 demonstrates that synapsis between homologous cores is initiated at few sites but advances rapidly relative to the establishment of new initiation sites. If the rapid advance of synapsis deters additional initiation sites between pairs of homologues, it may provide a mechanism for positive recombination interference. Immunogold epitope mapping of antibodies to four Syn1 fusion proteins places the amino terminus of Syn1 towards the centre of the synaptonemal complex while the carboxyl terminus extends well into the lateral domain of the synaptonemal complex. The Syn1 fusion proteins have a non-specific DNA binding capacity. Immunogold labelling of Cor1 antigens indicates that the lateral domain of the synaptonemal complex is about twice as wide as the apparent width of lateral elements when stained with electron-dense metal ions. Electron microscopy of shadow-cast surface-spread SCs confirms the greater width of the lateral domain. The implication of these dimensions is that the proteins that comprise the synaptic domain overlap with the protein constituents of the lateral domains of the synaptonemal complex more than was apparent from earlier observations. This arrangement suggests that direct interactions might be expected between some of the synaptonemal complex proteins.

scholarly journals Chromosome-autonomous feedback down-regulates meiotic DNA break competence upon synaptonemal complex formation

2020 ◽  
Vol 34 (23-24) ◽  
pp. 1605-1618 ◽  
Author(s):  
Xiaojing Mu ◽  
Hajime Murakami ◽  
Neeman Mohibullah ◽  
Scott Keeney
Keyword(s):  
Complex Formation ◽  
Synaptonemal Complex ◽  
Synaptonemal Complex Formation ◽  
Dna Break

Chromatin structure contribution to the synaptonemal complex formation

2008 ◽  
Vol 66 (7) ◽  
pp. 1198-1208 ◽  
Author(s):  
A. Hernández-Hernández ◽  
G. H. Vázquez-Nin ◽  
O. M. Echeverría ◽  
F. Recillas-Targa
Keyword(s):  
Complex Formation ◽  
Synaptonemal Complex ◽  
Chromatin Structure ◽  
Synaptonemal Complex Formation
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