High-resolution R-banding at the 1250-band level. II. Schematic representation and nomenclature of human RBG-banded chromosomes

Genome ◽  
1989 ◽  
Vol 32 (3) ◽  
pp. 425-439 ◽  
Author(s):  
Régen Drouin ◽  
Claude-Lise Richer
Keyword(s):  
High Resolution ◽  
Banding Pattern ◽  
Chromosome Banding ◽  
Chromosomal Rearrangements ◽  
Staining Intensity ◽  
Brdu Incorporation ◽  
Lateral Asymmetry ◽  
Schematic Representation ◽  
Band Contrast ◽  
Definition Of

Detailed characterization of the RBG-banding pattern at the 1250-band level has been done after thymidine synchronization and block release with 5-bromo-2′-deoxyuridine (BrdU), which induces chromosome elongation and improves definition of chromosomal bands. Optimal conditions for the incorporation of BrdU and the use of the FPG (fluorochrome–photolysis–Giemsa) technique produced excellent band separation and band contrast even in highly elongated prophase chromosomes. Moreover, we did not observe lateral asymmetry in C-banded regions. The schematic representation of these elongated chromosomes in the 1250-band range per haploid set was prepared showing the relative position, the specific size, and the characteristic staining intensity for each band. To this idiogram was extended the International Standard Cytogenetic Nomenclature. This realistic idiogram should help in the preparation of R-banded prophase karyotypes and in the identification and localization of chromosomal rearrangements. Because differences exist between RBG and RHG bands, a brief comparative description of each RBG-banded chromosome is included. Moreover, a minute analysis of the banding pattern revealed that various parts of chromosomes contract differently. We also observed the presence of R-positive bands in heterochromatic regions of the short arms of the acrocentrics, and of chromosomes 1, 9, 16, and Y.Key words: high-resolution chromosome banding, R-banding, idiogram, dynamic bandings, prophase chromosomes, chromosome banding by BrdU incorporation.

High-resolution idiogram of Giemsa R-banded human prophase chromosomes

1983 ◽  
Vol 25 (6) ◽  
pp. 642-650 ◽  
Author(s):  
C. L. Richer ◽  
R. Drouin ◽  
M. Murer-Orlando ◽  
P. Jean
Keyword(s):  
Comparative Analysis ◽  
High Resolution ◽  
Relative Position ◽  
Chromosomal Rearrangements ◽  
Staining Intensity ◽  
Chromosome Condensation ◽  
Giemsa Staining ◽  
Heat Denaturation ◽  
Schematic Representation ◽  
International Standard

The schematic representation of RHG-banded chromosomes (R-banding was produced by heat denaturation followed by Giemsa staining (RHG)) in the 850-band range per haploid set, was prepared showing the relative position, the specific size, and the characteristic staining intensity for each band. To this idiogram was adapted the new International Standard Cytogenetic Nomenclature. Our aim was to produce a realisitic idiogram which could help in the preparation of R-banded prophase karyotypes and in the localization of chromosomal rearrangements. A comparative analysis of bands at prophase and metaphase revealed certain aspects of the dynamics involved in chromosome condensation and in R-band organization. The effect of chromosome elongation on the appearance of R-bands within heterochromatic regions has also been discussed.

Idiograms of horse chromosomes at prometaphase, early metaphase, and midmetaphase after R-banding by BrdU incorporation followed by the fluorochrome–photolysis–Giemsa technique

Genome ◽  
1987 ◽  
Vol 29 (4) ◽  
pp. 674-679 ◽  
Author(s):  
April Romagnano ◽  
Régen Drouin ◽  
Claude-Lise Richer
Keyword(s):  
Key Words ◽  
Banding Pattern ◽  
Chromosome Banding ◽  
Brdu Incorporation ◽  
Domestic Horse ◽  
Cell Synchronization

We present three idiograms of equine chromosomes, R-banded after BrdU incorporation and stained by the fluorochrome–photolysis–Giemsa technique. The haploid set of prometaphasic chromosomes shows 591 bands (range 7–38 per chromosome), the early metaphasic set 404 (range 5–26), and the midmetaphasic set 272 (range 3–18). Following cell synchronization with thymidine, more than twice as many R-bands were revealed on the resulting prometaphasic chromosomes, making possible the establishment of a very accurate and characteristic representation of this banding pattern in the domestic horse. Key words: chromosome banding, horse, R-bands, prometaphase.

scholarly journals BIMG-04. MAPPING HETEROGENEITY OF HIGH-GRADE GLIOMA METABOLISM USING HIGH RESOLUTION 7T MRSI

2021 ◽  
Vol 3 (Supplement_1) ◽  
pp. i1-i1
Author(s):  
Gilbert Hangel ◽  
Cornelius Cadrien ◽  
Philipp Lazen ◽  
Sukrit Sharma ◽  
Julia Furtner ◽  
...  
Keyword(s):  
High Resolution ◽  
Imaging Techniques ◽  
Basis Set ◽  
Metabolic Imaging ◽  
Tumor Segmentation ◽  
Low Grade ◽  
High Grade ◽  
Isotropic Resolution ◽  
Contrast Enhanced ◽  
Definition Of

Abstract OBJECTIVES Neurosurgical resection in gliomas depends on the precise preoperative definition of the tumor and its margins to realize a safe maximum resection that translates into a better patient outcome. New metabolic imaging techniques could improve this delineation as well as designate targets for biopsies. We validated the performance of our fast high-resolution whole-brain 3D-magnetic resonance spectroscopic imaging (MRSI) method at 7T in high-grade gliomas (HGGs) as first step to this regard. METHODS We measured 23 patients with HGGs at 7T with MRSI covering the whole cerebrum with 3.4mm isotropic resolution in 15 min. Quantification used a basis-set of 17 neurochemical components. They were evaluated for their reliability/quality and compared to neuroradiologically segmented tumor regions-of-interest (necrosis, contrast-enhanced, non-contrast-enhanced+edema, peritumoral) and histopathology (e.g., grade, IDH-status). RESULTS We found 18/23 measurements to be usable and ten neurochemicals quantified with acceptable quality. The most common denominators were increases of glutamine, glycine, and total choline as well as decreases of N-acetyl-aspartate and total creatine over most tumor regions. Other metabolites like taurine and serine showed mixed behavior. We further found that heterogeneity in the metabolic images often continued into the peritumoral region. While 2-hydroxy-glutarate could not be satisfyingly quantified, we found a tendency for a decrease of glutamate in IDH1-mutant HGGs. DISCUSSION Our findings corresponded well to clinical tumor segmentation but were more heterogeneous and often extended into the peritumoral region. Our results corresponded to previous knowledge, but with previously not feasible resolution. Apart from glycine/glutamine and their role in glioma progression, more research on the connection of glutamate and others to specific mutations is necessary. The addition of low-grade gliomas and statistical ROI analysis in a larger cohort will be the next important steps to define the benefits of our 7T MRSI approach for the definition of spatial metabolic tumor profiles.

scholarly journals The definition of an atmospheric database for Aeolus

2011 ◽  
Vol 4 (1) ◽  
pp. 67-88 ◽  
Author(s):  
G. J. Marseille ◽  
K. Houchi ◽  
J. de Kloe ◽  
A. Stoffelen
Keyword(s):  
High Resolution ◽  
Meteorological Data ◽  
Sampling Strategy ◽  
Laser Wavelength ◽  
Geographic Region ◽  
Atmospheric Dynamics ◽  
Specific Humidity ◽  
Small Scale ◽  
Horizontal Line ◽  
Definition Of

Abstract. The definition of an atmospheric database is an important component of simulation studies in preparation of future earth observing remote sensing satellites. The Aeolus mission, formerly denoted Atmospheric Dynamics Mission (ADM) or ADM-Aeolus, is scheduled for launch end of 2013 and aims at measuring profiles of single horizontal line-of-sight (HLOS) wind components from the surface up to about 32 km with a global coverage. The vertical profile resolution is limited but may be changed during in-orbit operation. This provides the opportunity of a targeted sampling strategy, e.g., as a function of geographic region. Optimization of the vertical (and horizontal) sampling strategy requires a characterization of the atmosphere optical and dynamical properties, more in particular the distribution of atmospheric particles and their correlation with the atmospheric dynamics. The Aeolus atmospheric database combines meteorological data from the ECMWF model with atmosphere optical properties data from CALIPSO. An inverse algorithm to retrieve high-resolution particle backscatter from the CALIPSO level-1 attenuated backscatter product is presented. Global weather models tend to underestimate atmospheric wind variability. A procedure is described to ensure compatibility of the characteristics of the database winds with those from high-resolution radiosondes. The result is a high-resolution database of zonal, meridional and vertical wind, temperature, specific humidity and particle and molecular backscatter and extinction at 355 nm laser wavelength. This allows the simulation of small-scale atmospheric processes within the Aeolus observation sampling volume and their impact on the quality of the retrieved HLOS wind profiles. The database extends over four months covering all seasons. This allows a statistical evaluation of the mission components under investigation. The database is currently used for the development of the Aeolus wind processing, the definition of wind calibration strategies and the optimization of the Aeolus sampling strategy.

scholarly journals Chromosome banding pattern in a polymorphic population of Sorex araneus from northeastern Finland

Hereditas ◽  
2009 ◽  
Vol 76 (2) ◽  
pp. 305-314 ◽  
Author(s):  
LIISA HALKKA ◽  
O. HALKKA ◽  
U. SKARÉN ◽  
VERONICA SÖDERLUND
Keyword(s):  
Banding Pattern ◽  
Chromosome Banding ◽  
Sorex Araneus

1060: THE BERLIN DEFINITION OF ARDS SEVERITY IS ASSOCIATED WITH PROGNOSIS AND HIGH-RESOLUTION CT SCORES

2018 ◽  
Vol 46 (1) ◽  
pp. 514-514
Author(s):  
Tetsuro Kamo ◽  
Sadatomo Tasaka ◽  
Takeshi Suzuki ◽  
Takanori Asakura ◽  
Shouji Suzuki ◽  
...  
Keyword(s):  
High Resolution ◽  
Berlin Definition ◽  
High Resolution Ct ◽  
Definition Of

scholarly journals A numerical fit of analytical to simulated density profiles in dark matter haloes

2005 ◽  
pp. 13-32 ◽  
Author(s):  
R. Caimmi ◽  
C. Marmo ◽  
T. Valentinuzzi
Keyword(s):  
High Resolution ◽  
Density Profile ◽  
Geometrical Parameters ◽  
Density Profiles ◽  
Mean Values ◽  
Geometrical Properties ◽  
Best Fitting ◽  
Definition Of ◽  
Additional Support ◽  
Open Question

Analytical and geometrical properties of generalized power-law (GPL) density profiles are investigated in detail. In particular, a one-to-one correspondence is found between mathematical parameters (a scaling radius, r0, a scaling density, ?0, and three exponents, ?, ?, ?), and geometrical parameters (the coordinates of the intersection of the asymptotes, xC, yC, and three vertical intercepts, b, b?, b?, related to the curve and the asymptotes, respectively): (r0,?0,?,?,?) ? (xC,yC,b,b?,b?). Then GPL density profiles are compared with simulated dark haloes (SDH) density profiles, and nonlinear least-absolute values and least-squares fits involving the above mentioned five parameters (RFSM5 method) are prescribed. More specifically, the sum of absolute values or squares of absolute logarithmic residuals, Ri=log?SDH(ri) ? log?GPL(ri), is evaluated on 10 points making a 5dimension hypergrid, through a few iterations. The size is progressively reduced around a fiducial minimum, and superpositions on nodes of earlier hypergrids are avoided. An application is made to a sample of 17 SDHs on the scale of cluster of galaxies, within a flat ?CDM cosmological model (Rasia et al. 2004). In dealing with the mean SDH density profile, a virial radius, Rvir, averaged over the whole sample, is assigned, which allows the calculation of the remaining parameters. Using a RFSM5 method provides a better fit with respect to other methods. The geometrical parameters, averaged over the whole sample of best fitting GPL density profiles, yield (?, ?, ?) ? (0.6,3.1,1.0), to be compared with (?, ?, ?) = (1,3,1), i.e. the NFW density profile (Navarro et al. 1995, 1996, 1997), (?, ?, ?) = (1.5,3, 1.5) (Moore et al. 1998, 1999), (?, ?, ?) = (1,2.5,1) (Rasia et al. 2004); and, in addition, ? ? 1.5 (Hiotelis 2003), deduced from the application of a RFSM5 method, but using a different definition of scaled radius, or concentration; and ? ? 1.21.3 deduced from more recent high-resolution simulations (Diemand et al. 2004, Reed et al. 2005). No evident correlation is found between SDH dynamical state (relaxed or merging) and asymptotic inner slope of the fitting logarithmic density profile or (for SDH comparable virial masses) scaled radius. Mean values and standard deviations of some parameters are calculated, and in particular the decimal logarithm of the scaled radius, ?vir, reads < log?vir >= 0.74 and ?slog?vir = 0.150.17, consistent with previous results related to NFW density profiles. It provides additional support to the idea, that NFW density profiles may be considered as a convenient way to parametrize SDH density profiles, without implying that it necessarily produces the best possible fit (Bullock et al. 2001). A certain degree of degeneracy is found in fitting GPL to SDH density profiles. If it is intrinsic to the RFSM5 method or it could be reduced by the next generation of high-resolution simulations, still remains an open question. .

Synchronization of human leukemic cells: Relevance for high-resolution chromosome banding

1984 ◽  
Vol 66 (2-3) ◽  
pp. 220-224 ◽  
Author(s):  
J. H. Gallo ◽  
J. V. Ordonez ◽  
G. E. Brown ◽  
J. R. Testa
Keyword(s):  
High Resolution ◽  
Chromosome Banding ◽  
Leukemic Cells ◽  
Human Leukemic Cells

AN AUTOMATIC METHOD OF COMPILATION AND MAPPING OF HIGH‐RESOLUTION AEROMAGNETIC DATA

Geophysics ◽  
1971 ◽  
Vol 36 (4) ◽  
pp. 695-716 ◽  
Author(s):  
B. K. Bhattacharyya
Keyword(s):  
High Resolution ◽  
Magnetic Measurements ◽  
High Sensitivity ◽  
Positional Error ◽  
Automatic Method ◽  
Aeromagnetic Data ◽  
Aeromagnetic Survey ◽  
Machine Method ◽  
Flux Gate ◽  
Definition Of

An automatic method has been developed for compilation of digital aeromagnetic data. This method has been applied to the data obtained during a high‐sensitivity aeromagnetic survey over an area in the Precambrian shield of northeastern Ontario in Canada. With this method, all points of intersection between traverse and base lines are determined automatically and adjusted within the limits of positional error for minimizing differences in magnetic values at the intersections. Then the data are corrected for diurnal variation and leveled to tie the magnetic measurements together. Next, the resulting total field values are contoured with a machine method at a scale of 1:25,000. For such a scale, the minimum contour interval that can be used in the present area is two gammas. However, because of the accuracy of the method of compilation, with a larger scale, it is possible to trace one‐gamma contours. The maps thus compiled have been compared with published aeromagnetic maps of data obtained with conventional flux‐gate and proton‐precession magnetometers. The new maps are vastly superior to the old ones for delineating trends, patterns, and fine features of available detailed geological maps. This superiority is mainly due to the excellent definition of small amplitude anomalies, some of only a few gammas in magnitude, on the high‐resolution magnetic maps.

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