The role of a conserved dodecamer sequence in yeast mitochondrial gene expression

Genome ◽  
1989 ◽  
Vol 31 (2) ◽  
pp. 757-760 ◽  
Author(s):  
Ronald A. Butow ◽  
Hong Zhu ◽  
Philip Perlman ◽  
Heather Conrad-Webb
Keyword(s):  
Gene Expression ◽  
Rna Processing ◽  
Mitochondrial Gene ◽  
Rna Stability ◽  
Rrna Gene ◽  
Mitochondrial Gene Expression ◽  
Reading Frame ◽  
Multicomponent Complex ◽  
Var1 Gene

All mRNAs on the yeast mitochondrial genome terminate at a conserved dodecamer sequence 5′-AAUAAUAUUCUU-3′. We have characterized two mutants with altered dodecamers. One contains a deletion of the dodecamer at the end of the var1 gene, and the other contains two adjacent transversions in the dodecamer at the end of the reading frame of fit1, a gene within the ω+ allele of the 21S rRNA gene. In each mutant, expression of the respective gene is blocked completely. A dominant nuclear suppressor, SUV3-1, was isolated that suppresses the var1 deletion but is without effect on the fit1 dodecamer mutations. Unexpectedly, however, we found that SUV3-1 blocks expression of the wild-type fit1 allele by blocking processing at its dodecamer. SUV3-1 has pleiotropic effects on mitochondrial gene expression, affecting RNA processing, RNA stability, and translation. Our results suggest that RNA metabolism and translation may be part of a multicomponent complex within mitochondria.Key words: mitochondria, yeast, mRNA, RNA processing, 3′ dodecamer.

scholarly journals Gene expression in plant mitochondria: transcriptional and post–transcriptional control

2003 ◽  
Vol 358 (1429) ◽  
pp. 181-189 ◽  
Author(s):  
Stefan Binder ◽  
Axel Brennicke
Keyword(s):  
Gene Expression ◽  
Rna Processing ◽  
Transcriptional Control ◽  
Mitochondrial Gene ◽  
Plant Mitochondria ◽  
Rna Stability ◽  
Mitochondrial Gene Expression ◽  
Evolutionary Origins ◽  
Cell Gene Expression ◽  
Cell Gene

The informational content of the mitochondrial genome in plants is, although small, essential for each cell. Gene expression in these organelles involves a number of distinct transcriptional and post–transcriptional steps. The complex post–transcriptional processes of plant mitochondria such as 5′ and 3′ RNA processing, intron splicing, RNA editing and controlled RNA stability extensively modify individual steady–state RNA levels and influence the mRNA quantities available for translation. In this overview of the processes in mitochondrial gene expression, we focus on confirmed and potential sites of regulatory interference and discuss the evolutionary origins of the transcriptional and post–transcriptional processes.

Regulation of mitochondrial gene expression in Saccharomyces cerevisiae: the role of RNA-binding enzymes

2001 ◽  
Vol 1 (S1) ◽  
Author(s):  
H van der Spek ◽  
M Siep ◽  
L de Jong ◽  
SDJ Elzinga ◽  
K van Oosterum ◽  
...  
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Rna Binding ◽  
Mitochondrial Gene ◽  
Mitochondrial Gene Expression

A Role of P300 in Post-natal Cardiac Mitochondrial Gene Expression and Function

2006 ◽  
Vol 12 (8) ◽  
pp. S162-S163
Author(s):  
Yasuaki Nakagawa ◽  
Koichiro Kuwahara ◽  
Masaki Harada ◽  
Genzo Takemura ◽  
Masaharu Akao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mitochondrial Gene ◽  
Mitochondrial Gene Expression ◽  
And Function

Regulation of cyclin D1 gene expression

2010 ◽  
Vol 38 (1) ◽  
pp. 217-222 ◽  
Author(s):  
Ini-Isabée Witzel ◽  
Li Fang Koh ◽  
Neil D. Perkins
Keyword(s):  
Gene Expression ◽  
Cyclin D1 ◽  
Rna Processing ◽  
Dominant Role ◽  
Rna Stability ◽  
Transcriptional Regulator ◽  
Nuclear Factor Κb ◽  
Nuclear Factor ◽  
Post Transcriptional Regulation

Cyclin D1 is a key regulator of cell proliferation and its expression is subject to both transcriptional and post-transcriptional regulation. In different cellular contexts, different pathways assume a dominant role in regulating its expression, whereas their disregulation can contribute to overexpression of cyclin D1 in tumorigenesis. Here, we discuss the ability of the NF-κB (nuclear factor κB)/IKK [IκB (inhibitor of NF-κB) kinase] pathways to regulate cyclin D1 gene transcription and also consider the newly discovered role of the SNARP (SNIP1/SkIP-associated RNA processing) complex as a co-transcriptional regulator of cyclin D1 RNA stability.

scholarly journals The translational activator Sov1 coordinates mitochondrial gene expression with mitoribosome biogenesis

2020 ◽  
Vol 48 (12) ◽  
pp. 6759-6774 ◽  
Author(s):  
Suhas R Seshadri ◽  
Chitra Banarjee ◽  
Mario H Barros ◽  
Flavia Fontanesi
Keyword(s):  
Gene Expression ◽  
Functional Role ◽  
Regulatory Mechanism ◽  
Mitochondrial Gene ◽  
Small Subunit ◽  
Metabolic Process ◽  
Mitochondrial Gene Expression ◽  
Var1 Gene ◽  
Bona Fide ◽  
Translational Activator

Abstract Mitoribosome biogenesis is an expensive metabolic process that is essential to maintain cellular respiratory capacity and requires the stoichiometric accumulation of rRNAs and proteins encoded in two distinct genomes. In yeast, the ribosomal protein Var1, alias uS3m, is mitochondrion-encoded. uS3m is a protein universally present in all ribosomes, where it forms part of the small subunit (SSU) mRNA entry channel and plays a pivotal role in ribosome loading onto the mRNA. However, despite its critical functional role, very little is known concerning VAR1 gene expression. Here, we demonstrate that the protein Sov1 is an in bona fide VAR1 mRNA translational activator and additionally interacts with newly synthesized Var1 polypeptide. Moreover, we show that Sov1 assists the late steps of mtSSU biogenesis involving the incorporation of Var1, an event necessary for uS14 and mS46 assembly. Notably, we have uncovered a translational regulatory mechanism by which Sov1 fine-tunes Var1 synthesis with its assembly into the mitoribosome.

Role of vitamin A in mitochondrial gene expression

2001 ◽  
Vol 54 ◽  
pp. S11-S27 ◽  
Author(s):  
Carolyn D Berdanier ◽  
Helen B Everts ◽  
Christina Hermoyian ◽  
Clayton E Mathews
Keyword(s):  
Gene Expression ◽  
Vitamin A ◽  
Mitochondrial Gene ◽  
Mitochondrial Gene Expression

RNA processing and expression of an intron-encoded protein in yeast mitochondria: role of a conserved dodecamer sequence

1987 ◽  
Vol 7 (7) ◽  
pp. 2530-2537 ◽  
Author(s):  
H Zhu ◽  
I G Macreadie ◽  
R A Butow
Keyword(s):  
Rna Processing ◽  
Rrna Gene ◽  
Yeast Mitochondria ◽  
Wild Type ◽  
Reading Frame ◽  
Processing Activity ◽  
Novel Processing ◽  
Type Strains ◽  
Upstream Exon

The 3' ends of most Saccharomyces cerevisiae mitochondrial mRNAs terminate at a conserved dodecamer sequence, 5'-AAUAAUAUUCUU-3', of unknown function. We have studied the consequences of mutations within a dodecamer found in an 1,143-base-pair optional intron of the mitochondrial large (21S) rRNA gene on RNA processing. The dodecamer is situated at the 3' end of an expressed open reading frame (ORF) within that intron, and the mutations are two adjacent transversions that extend the intron ORF by 51 nucleotides. The strain harboring these mutations, L5-10-1, is defective in biased intron transmission in crosses to strains that lack the intron, as are other mutants which contain nucleotide changes within the ORF (I. G. Macreadie, R. M. Scott, A. R. Zinn, and R. A. Butow, Cell 41:395-402, 1985). However, unlike these other mutants, wild-type strains, or petites which retain the intron allele, L5-10-1 is defective in processing at the intron dodecamer. In addition, L5-10-1 lacks a prominent 2.7-kilobase RNA containing both intron and exon sequences and at least two of four RNAs that correspond to various forms of the excised intron. We propose that these RNAs, missing in L5-10-1 but present in all other strains examined, arise in part by processing at the intron dodecamer. In addition, in all strains examined, we have detected a novel processing activity in which precursor 21S rRNA transcripts are cleaved in the upstream exon, about 1,500 nucleotides from the 5' end of the RNA. This activity, together with 3' intron dodecamer cleavage, probably accounts for the 2.7-kilobase RNA species, a candidate for the mRNA for the intron-encoded protein.

PS1-58 The role of STAT1 in regulation of mitochondrial gene expression

Cytokine ◽  
2010 ◽  
Vol 52 (1-2) ◽  
pp. 29
Author(s):  
Jennifer D. Sisler ◽  
Magdalena Szelag ◽  
Ramesh Potla ◽  
Qifang Zhang ◽  
Karol Szczepanek ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mitochondrial Gene ◽  
Mitochondrial Gene Expression
Sign in / Sign up

Export Citation Format

Share Document