Can abortive early homologous associations promote increased crossing-over in an adjacent rearranged segment?

Genome ◽  
1988 ◽  
Vol 30 (4) ◽  
pp. 469-472 ◽  
Author(s):  
Marjorie P. Maguire
Keyword(s):  
Crossing Over ◽  
Meiotic Pairing ◽  
Pairing Behavior ◽  
Chromosome Segments

Meiotic pairing behavior of rearranged chromosome segments is compared across an informative series of combinations. The question is raised whether the simplest explanation for some peculiar findings may include a sequence of synaptic precursor events at leptotene or zygotene, the course of which may eventually strongly affect crossover frequency.Key words: meiosis, synapsis, crossing-over, rearrangement.

Effect of the Pairing Gene Ph1 and Premeiotic Colchicine Treatment on Intra- and Interchromosome Pairing of Isochromosomes in Common Wheat

Genetics ◽  
1998 ◽  
Vol 150 (3) ◽  
pp. 1199-1208 ◽  
Author(s):  
Juan M Vega ◽  
Moshe Feldman
Keyword(s):  
Common Wheat ◽  
Homologous Chromosome ◽  
Colchicine Treatment ◽  
Crossing Over ◽  
Meiotic Pairing ◽  
Factors Affecting ◽  
Homologous Chromosomes ◽  
Polyploid Wheat ◽  
Main Gene ◽  
Ph1 Gene

Abstract The analysis of the pattern of isochromosome pairing allows one to distinguish factors affecting presynaptic alignment of homologous chromosomes from those affecting synapsis and crossing-over. Because the two homologous arms in an isochromosome are invariably associated by a common centromere, the suppression of pairing between these arms (intrachromosome pairing) would indicate that synaptic or postsynaptic events were impaired. In contrast, the suppression of pairing between an isochromosome and its homologous chromosome (interchromosome pairing), without affecting intrachromosome pairing, would suggest that homologous presynaptic alignment was impaired. We used such an isochromosome system to determine which of the processes associated with chromosome pairing was affected by the Ph1 gene of common wheat—the main gene that restricts pairing to homologues. Ph1 reduced the frequency of interchromosome pairing without affecting intrachromosome pairing. In contrast, intrachromosome pairing was strongly reduced in the absence of the synaptic gene Syn-B1. Premeiotic colchicine treatment, which drastically decreased pairing of conventional chromosomes, reduced interchromosome but not intrachromosome pairing. The results support the hypothesis that premeiotic alignment is a necessary stage for the regularity of meiotic pairing and that Ph1 relaxes this alignment. We suggest that Ph1 acts on premeiotic alignment of homologues and homeologues as a means of ensuring diploid-like meiotic behavior in polyploid wheat.

scholarly journals A cis-acting locus that promotes crossing over between X chromosomes in Caenorhabditis elegans.

Genetics ◽  
1994 ◽  
Vol 136 (3) ◽  
pp. 887-902 ◽  
Author(s):  
A M Villeneuve
Keyword(s):  
Caenorhabditis Elegans ◽  
X Chromosome ◽  
Sharp Decrease ◽  
Crossing Over ◽  
Meiotic Pairing ◽  
X Chromosomes ◽  
Cis Acting ◽  
Pairing Site ◽  
High Level ◽  
Pairing Center

Abstract This study reports the characterization of a cis-acting locus on the Caenorhabditis elegans X chromosome that is crucial for promoting normal levels of crossing over specifically between the X homologs and for ensuring their proper disjunction at meiosis I. The function of this locus is disrupted by the mutation me8, which maps to the extreme left end of the X chromosome within the region previously implicated by studies of X; A translocations and X duplications to contain a meiotic pairing site. Hermaphrodites homozygous for a deletion of the locus (Df/Df) or heterozygous for a deletion and the me8 mutation (me8/Df) exhibit extremely high level of X chromosome nondisjunction at the reductional division; this is correlated with a sharp decrease in crossing over between the X homologs as evidenced both by reductions in genetic map distances and by the presence of achiasmate chromosomes in cytological preparations of oocyte nuclei. Duplications of the wild-type region that are unlinked to the X chromosome cannot complement the recombination and disjunction defects in trans, indicating that this region must be present in cis to the X chromosome to ensure normal levels of crossing over and proper homolog disjunction. me8 homozygotes exhibit an altered distribution of crossovers along the X chromosome that suggests a defect in processivity along the X chromosome of an event that initiates at the chromosome end. Models are discussed in which the cis-acting locus deleted by the Dfs functions as a meiotic pairing center that recruits trans-acting factors onto the chromosomes to nucleate assembly of a crossover-competent complex between the X homologs. This pairing center might function in the process of homolog recognition, or in the initiation of homologous synapsis.

scholarly journals PAIRING COMPETITION BETWEEN IDENTICAL AND HOMOLOGOUS CHROMOSOMES IN AUTOTETRAPLOID RYE. I. SUBMETACENTRIC CHROMOSOMES

Genetics ◽  
1985 ◽  
Vol 111 (4) ◽  
pp. 933-944
Author(s):  
J Orellana ◽  
J L Santos
Keyword(s):  
Genetic Control ◽  
Secale Cereale ◽  
Inbred Lines ◽  
Meiotic Pairing ◽  
Homologous Chromosomes ◽  
Partner Exchange ◽  
Pairing Behavior ◽  
Chromosome 1R ◽  
Heterochromatin Content

ABSTRACT Meiotic pairing preferences between identical and homologous but not identical chromosomes were analyzed in ten induced tetraploid/diploid chimaeral rye plants (Secale cereale) heterozygous for telomeric heerochromatin C-bands in both arms of chromosome 1R. These plants were the progeny of two crosses between only one plant of cv. Petkus, used as male, and two plants of the inbred lines E and R, respectively. Different pairing preferences for chromosome 1R were found: (1) between plants, (2) between chromosome arms within the same plant and (3) between bivalents and multivalents within the same plant. The possible influence in the preferences of several factors such as differences in C-heterochromatin content in the chromosomes analyzed, specific genetic control and independence in pairing behavior between both arms and partner exchange is discussed.

scholarly journals RECONSTITUTION OF THE Rr COMPOUND ALLELE IN MAIZE

Genetics ◽  
1974 ◽  
Vol 78 (2) ◽  
pp. 691-701
Author(s):  
H K Dooner ◽  
J L Kermicle
Keyword(s):  
Homologous Chromosome ◽  
Tandem Duplication ◽  
Crossing Over ◽  
Anthocyanin Pigmentation ◽  
Genetic Length ◽  
Chromosome Segments

ABSTRACT The Rr:standard allele in maize, which conditions anthocyanin pigmentation in plant and seed tissues in the presence of appropriate complementary factors, is associated with a tandem duplication. The proximal member of the duplication carries P, the plant pigmenting determiner and the distal member member carries S, the seed pigmenting determiner. Derivatives from Rr that have lost S function are designated rr. They represent either losses of the distal member of the duplication (P derivatives) or mutations of S to s (P s). Derivatives that have lost P function are designated Rg, and represent either losses of the proximal member of the duplication (S derivatives) or mutations of P to p (p S).—All four possible types of rr/Rg heterozygotes were tested for their capacity to yield Rr reconstitution by crossing over. No Rr derivatives were obtained from P/S heterozygotes, a result consistent with the view that P and S occupy corresponding positions in homologous chromosome segments. Rr reconstitution was detected in both tandem duplication heterozygotes P s/S and P/p S, and was found to be about ten times more frequent in the latter. The ratio of Rr reconstitution in the two heterozygotes is a function of position of the anthocyanin marker within the duplicated segment. The data from these heterozygotes allow one to measure the distance between P and S, that is to say, the genetic length of the duplicated segment. This distance was found to be 0.16 map units. The highest frequency of Rr reconstitution was obtained from P s/p S heterozygotes, since direct pairing (see PDF) as well as the p//s type of displaced pairing have the potential to produce Rr derivatives. One of the Rg derivatives used in this study, Rg  6, was found to back-mutate in some sublines to Rr. The basis for this instability remains unknown.

Molecular Cytogenetic Analysis and Meiotic Pairing Behavior of Progenies Originating from a Hexaploid Triticale (×Triticosecale, Wittmack) and Bread Wheat (Triticum aestivum, L.) Cross

2020 ◽  
Vol 160 (1) ◽  
pp. 47-56
Author(s):  
Aybeniz J. Aliyeva ◽  
András Farkas ◽  
Naib Kh. Aminov ◽  
Klaudia Kruppa ◽  
Márta Molnár-Láng ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Chromosome Pairing ◽  
Addition Line ◽  
Meiotic Pairing ◽  
Hexaploid Triticale ◽  
Substitution Lines ◽  
Molecular Cytogenetic ◽  
Triticale Line ◽  
Pairing Behavior

The chromosomal constitution of 9 dwarf (D) and 8 semidwarf (SD) lines derived by crossing hexaploid Triticale line NA-75 (AABBRR, 2n = 6x = 42) with Triticumaestivum (AABBDD, 2n = 6x = 42) cv. Chinese Spring was investigated using molecular cytogenetic techniques: fluorescence in situ hybridization and genomic in situ hybridization. A wheat-rye translocation (T4DS.7RL), 8 substitution lines, and a ditelosomic addition line (7RSdt) were identified. In the substitution lines, 1, 2, or 4 pairs of wheat chromosomes, belonging to the A, B, or D genome, were replaced by rye chromosomes. Substitutions between chromosomes belonging to different wheat genomes [5B(5A), 1D(1B)] also occurred. The lines were genetically stable, each carrying 42 chromosomes, except the wheat-rye ditelosomic addition line, which carried 21 pairs of wheat chromosomes and 1 pair of rye telocentric chromosomes (7RS). The chromosome pairing behavior of the lines was studied during metaphase I of meiosis. The chromosome pairing level and the number of ring bivalents were different for each line. Besides rod bivalents, univalent and multivalent associations (tri- and quadrivalents) were also detected. The main goal of the experiment was to develop genetically stable wheat/Triticale recombinant lines carrying chromosomes/chromatin fragments originating from the R genome of Triticale line NA-75. Introgression of rye genes into hexaploid wheat can broaden its genetic diversity, and the newly developed lines can be used in wheat breeding programs.

Origin, structure, and behavior of a highly rearranged deletion chromosome 1BS-4 in wheat

Genome ◽  
2005 ◽  
Vol 48 (4) ◽  
pp. 591-597 ◽  
Author(s):  
Lili Qi ◽  
Bernd Friebe ◽  
Bikram S Gill
Keyword(s):  
Triticum Aestivum ◽  
Molecular Markers ◽  
Physical Mapping ◽  
Triticum Aestivum L ◽  
Distal Segment ◽  
Distal Region ◽  
Meiotic Pairing ◽  
Aegilops Species ◽  
Pairing Behavior ◽  
And Behavior

Wheat (Triticum aestivum L.) deletion (del) stocks are valuable tools for the physical mapping of molecular markers and genes to chromosome bins delineated by 2 adjacent deletion breakpoints. The wheat deletion stocks were produced by using gametocidal genes derived from related Aegilops species. Here, we report on the origin, structure, and behavior of a highly rearranged chromosome 1BS-4. The cytogenetic and molecular marker analyses suggest that 1BS-4 resulted from 2 breakpoints in the 1BS arm and 1 breakpoint in the 1BL arm. The distal segment from 1BS, except for a small deleted part, is translocated to the long arm. Cytologically, chromosome 1BS-4 is highly stable, but shows a unique meiotic pairing behavior. The short arm of 1BS-4 fails to pair with a normal 1BS arm because of lack of homology at the distal ends. The long arm of 1BS-4 only pairs with a normal 1BS arm within the distal region translocated from 1BS. Therefore, using the 1BS-4 deletion stock for physical mapping will result in the false allocation of molecular markers and genes proximal to the breakpoint of 1BS-4.Key words: Triticum aestivum, wheat, deletion–translocation, physical mapping.

Variation in "Map Distance" in Neurospora crassa

1969 ◽  
Vol 15 (6) ◽  
pp. 623-627 ◽  
Author(s):  
Mary B. Mitchell
Keyword(s):  
Life Cycle ◽  
Neurospora Crassa ◽  
Crossing Over ◽  
General Behavior ◽  
Phenotype Distribution ◽  
Random Distributions ◽  
Detailed Interpretation ◽  
Chromosome Segments ◽  
Map Distance

Determinations of the same map distance in different crosses often give divergent results, whereas values for unrelated map regions are often strikingly similar. This situation has been examined in samples of serially isolated spore octets, mainly from two-marker crosses. Observed ratios of phenotype distribution classes suggest more systematic modifications of random distributions than would be expected from reassociations, through crossing-over, of chromosome segments chosen unsystematically. Detailed interpretation appears impracticable, since complexities in the general behavior of the organism indicate that the ratios observed may reflect opportunities, occurring at different stages of the life cycle, for successive reassociations of genetic units.

A cytological study of intraspecific variation in Ceratopteris thalictroides

1979 ◽  
Vol 57 (16) ◽  
pp. 1694-1700 ◽  
Author(s):  
Leslie G. Hickok
Keyword(s):  
Spore Germination ◽  
Cytological Study ◽  
Meiotic Pairing ◽  
Spore Viability ◽  
Polymorphic Species ◽  
Structural Differences ◽  
Pairing Behavior ◽  
Ceratopteris Thalictroides ◽  
Low Levels ◽  
Intraspecific Hybrids

A cytological study of meiotic pairing behavior in intraspecific hybrids was undertaken utilizing seven different collections of Ceratopteris thalictroides. Two mutant gametophyte stocks with nonfunctional spermatozoids were utilized to facilitate the synthesis of intraspecific hybrids. Low levels of univalent and quadrivalent formation were found in most of the hybrids, indicating that some structural differences existed, but major pairing homologies were still evident. The fertility of hybrids ranged from 10 to 75% spore germination. One collection, from Hawaii, showed high levels of univalent formation and very low spore viability. In that this stock is both biologically and morphologically distinct, it should be given taxonomic recognition. The study, in general, is in agreement with the most recent taxonomic treatment of Ceratopteris in that it shows a significant amount of cytological variation within the polymorphic species C. thalictroides.

A stably transmitted pair of translocated supernumerary chromosomes in maize

Genome ◽  
1995 ◽  
Vol 38 (3) ◽  
pp. 558-565 ◽  
Author(s):  
Marjorie P. Maguire
Keyword(s):  
Chiasma Formation ◽  
B Chromosome ◽  
Supernumerary Chromosome ◽  
Chromosome Segment ◽  
Crossing Over ◽  
Preferential Pairing ◽  
Supernumerary Chromosomes ◽  
Unknown Source ◽  
Chromosome Segments

A pair of stably transmitted supernumerary chromosomes of unknown source has been found in a maize stock carrying a desynaptic mutant. The presence of the supernumerary chromosome appears to be unrelated to the meiotic mutant, but is believed to have been derived from a translocated B chromosome contaminant. The supernumerary chromosomes carry a segment of a A chromosome in this stock where there appear to be two normal copies of each of the 10 A chromosomes. Thus, this A chromosome segment is present in quadruplicate. Surprisingly, a quadrivalent configuration is formed in most microsporocytes, which involves not only synapsis but also chiasma formation in the A chromosome segments involved in the quadrivalent. This represents a strong preferential pairing of supernumeraries with the normal A chromosome segments. Such nonrandom association and crossing over might provide information on the nature of early homologue alignment at meiosis.Key words: supernumerary chromosomes, modified B chromosome, BA translocation, preferential pairing and crossing over.

Sign in / Sign up

Export Citation Format

Share Document