Inheritance and expression of tissue-specific catalase activity during development and aging in mice

Genome ◽  
1987 ◽  
Vol 29 (5) ◽  
pp. 748-760 ◽  
Author(s):  
N. J. Schisler ◽  
S. M. Singh
Keyword(s):  
Enzyme Activity ◽  
Catalase Activity ◽  
Inbred Mice ◽  
Age Groups ◽  
Activity Level ◽  
Genetic Determinants ◽  
Eukaryotic Gene ◽  
Allelic Differences ◽  
Development And Aging ◽  
Key Words Catalase

The catalase activity in the liver, kidney, lung, and blood hemolysate was measured in newborn, 21-, 70-, 175-, and >400-day-old mice from the strains BALB/c, Csb, C3H/HeSnJ, C3H/S, C57BL/6J, SW, and 129/ReJ. Catalase activity was found to be highest in the liver (~0.33 U/mg protein) followed by the kidney (~0.13 U/mg protein), lung (~0.05 U/mg protein), and blood hemolysate (~0.03 U/mg protein). ANOVA analysis indicated significant differences in enzyme activity among strains and age groups studied. The developmental profiles of enzyme activity were tissue and strain specific. Catalase activity in the blood, for example, was generally higher at birth and at old age, whereas the kidney catalase activity was low at birth and increased substantially with age. Strains could be classified as normal (129/ReJ, BALB/c, C3H/HeSnJ, C3H/S), hypocatalasemic (C57BL/6J, SW), and acatalasemic (Csb) with respect to enzyme activity and it was on this basis that the inheritance of the catalase phenotype was studied using appropriate crosses. The enzyme activity level in each tissue appears to be governed by a unique set of genetic regulators/modifiers that interact with a single structural gene (Cs) or its product to produce the catalase phenotype. Some of these (e.g., Ce-1 and Ce-2) have been previously described but based on the results of various crosses reported here, more must exist that remain still uncharacterized at the molecular level. Models proposed for the inheritance of the catalase phenotype vary in complexity from single allelic differences between strains (e.g., BALB/c × Csb; blood) to a system of multiple interacting genetic determinants (e.g., BALB/c × Csb; liver) each having dominant (e.g., C57BL/6J over BALB/c; liver) and recessive components (e.g., gene(s) conferring the acatalasemic phenotype in BALB/c × Csb; blood and kidney). Such results are important and offer an interesting model to further characterize aspects of eukaryotic gene regulation. Key words: catalase, inbred mice, tissue specificity, developmental profile, inheritance.

Personality's Correlates of Adult Development and Aging

2003 ◽  
Vol 8 (3) ◽  
pp. 131-147 ◽  
Author(s):  
Gian Vittorio Caprara ◽  
Mariagiovanna Caprara ◽  
Patrizia Steca
Keyword(s):  
Personality Traits ◽  
Adult Development ◽  
Self Efficacy ◽  
Age Groups ◽  
Well Being ◽  
Efficacy Beliefs ◽  
Cross Sectional ◽  
Education And Health ◽  
Development And Aging ◽  
And Gender

Three cross-sectional studies examined stability and change in personality over the course of life by measuring the relations linking age to personality traits, self-efficacy beliefs, values, and well-being in large samples of Italian male and female participants. In each study, relations between personality and age were examined across several age groups ranging from young adulthood to old age. In each study, personality constructs were first examined in terms of mean group differences accrued by age and gender and then in terms of their correlations with age across gender and age groups. Furthermore, personality-age correlations were also calculated, controlling for the demographic effects accrued by marital status, education, and health. Findings strongly indicated that personality functioning does not necessarily decline in the later years of life, and that decline is more pronounced in males than it is in females across several personality dimensions ranging from personality traits, such as emotional stability, to self-efficacy beliefs, such as efficacy in dealing with negative affect. Findings are discussed in terms of their implications for personality theory and social policy.

scholarly journals EFFECT ON 6-BAP CATALASE ACTIVITY IN TOMATO PLANTS UNDER CONDITIONS OF TEMPERATURE STRESS

2015 ◽  
Vol 4 (2) ◽  
pp. 96-99
Author(s):  
Tatyana Stepanovna Kolmykova ◽  
Ekaterina Vladimirovna Klokova ◽  
Elvera Shagidulovna Sharkaeva
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Enzymes ◽  
Environmental Factors ◽  
Catalase Activity ◽  
Low Temperatures ◽  
High Ability ◽  
Tomato Plants ◽  
Resistance To Stress ◽  
Primary Antioxidant ◽  
A Minor

Activity of the antioxidant system is one of the mechanisms for the protection of plants against adverse environmental factors. Catalase - a primary antioxidant enzymes. Her change may serve as an indicator of plant resistance to stress. Studied catalase activity in tomato plants of different varieties under the action of low temperatures and cytokinin 6-BAP preparation. The object of investigation used 24- and 27-day-old tomato plant varieties Podarochnyi, Patrice, Volgogradskyi. Found that under the action of low temperatures, the decrease in positive catalase activity: 10-30% at 10 C and 40-60% at a temperature of 3 C as compared with non-refrigerated plants. Less resistant to hyperthermia were plant varieties Patrice. With increasing length of vegetation at a temperature of 25 C in 27-day-old tomato plants resulted in a minor increase in the activity of the enzyme. After the end of the cooling observed recovery of enzyme activity only at grades Podarochnyi and Patrice. This indicates that the indicated tomato varieties possess a high ability to restore metabolic processes. Using 6-regulator cytokinin BAP increased catalase activity in tomato plants as prolonged or momentary cooling. Especially responsive to the drug were plant varieties Patrice and Volgogradskyi. And 6-BAP helped repair catalase activity in 27-day-old plants in the aftereffect of cold stress. Were more sensitive plant varieties Podarochnyi.

scholarly journals Pengaruh Paparan Hipoksia terhadap Aktivitas Antioksidan Katalase dan Kadar Malondialdehid (MDA) pada Jaringan Hati Tikus

Jurnal BIOMA ◽  
2014 ◽  
Vol 10 (2) ◽  
pp. 27
Author(s):  
Rini Puspitaningrum ◽  
Amanda Putri Lestari ◽  
Tri Murtiati
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Cell Membrane ◽  
Rat Liver ◽  
Catalase Activity ◽  
Liver Tissue ◽  
Hypoxia Exposure ◽  
Randomized Design ◽  
Mda Content ◽  
Key Words Catalase

Abstract content in the tissue. Hypoxia can make the formation of free radicals or reactive oxygen species (ROS) which reactive to cell membrane. Body will avoid free radicals by producing antioxidant, such as catalase enzyme. The reaction between ROS and cell membrane will form malondialdehyde (MDA). Liver is the main location of catalase. This research was aimed to know the influence of hypoxia exposure toward catalase antioxidant activity and MDA content in the rat liver tissue. This research used experiment method with fully randomized design. Based on one way Anova test (p≤0.01), it was shown that there had no average difference on catalase activity and MDA content toward length hypoxia exposure. The conclusion of this research was no influence of hypoxia exposure toward catalase activity and MDA content in rat liver tissue.   Key words: catalase antioxidant activity, hypoxia, malondialdehyde (MDA) content,rat liver tissue

Randomized Comparison of IV PEG and IM E. Coli Asparaginase in Children and Adolescents with Acute Lymphoblastic Leukemia: Results of the DFCI ALL Consortium Protocol 05-01

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 874-874 ◽  
Author(s):  
Lewis B. Silverman ◽  
Kristen Stevenson ◽  
Lynda M Vrooman ◽  
Jeffrey G Supko ◽  
Barbara Asselin ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Children And Adolescents ◽  
Lymphoblastic Leukemia ◽  
Age Groups ◽  
Serum Samples ◽  
Childhood All ◽  
E Coli ◽  
Significant Difference ◽  
Consolidation Phase

Abstract Abstract 874 E. coli L-asparaginase (E. coli ASP) is an important component of treatment for childhood ALL, but is associated with multiple toxicities, including allergy, pancreatitis, and thrombosis. It is typically given intramuscularly (IM). Because most pediatric ALL patients have indwelling venous catheters, intravenous (IV) administration of asparaginase would be a more convenient and less painful option than IM injection. PEG-asparaginase (PEG), the polyethylene glycol conjugate of E. coli ASP, has a longer circulating half-life and so may be given less frequently. We have previously demonstrated that a single dose of PEG 2500 IU/m2 given IV is tolerable in children with ALL, with potentially therapeutic serum enzyme activity (≥ 0.1 IU/mL) maintained for at least 18 days in most patients.[Blood 2010;115:1351-3] On DFCI ALL Consortium Protocol 05-01, all patients (pts) with newly diagnosed ALL aged 1–18 years (yrs) who achieved complete remission were eligible to participate in a randomized comparison of IM E. coli ASP and IV PEG during the 30-week (wk) multi-agent post-induction Consolidation phase. Beginning at week 7 of therapy, pts received either IM E. Coli ASP 25000 IU/m2 weekly × 30 wks or IV PEG 2500 IU/m2 every 2 wks × 30 wks. Serum samples were obtained every 6 wks just prior to an ASP dose and were assayed for ASP enzyme activity by a validated biochemical assay. Between 2005–2010, 463 pts were enrolled in the randomized comparison. Median age was 5 yrs (range 1.2–17.9 yrs). There was no significant difference in presenting characteristics between the two arms, except that more pts on the E. coli ASP arm presented with a mediastinal mass (9% vs 3%, p=0.04). Median follow-up was 2.8 years. Median nadir serum ASP activity (NSAA) at each assayed timepoint during the Consolidation phase was significantly higher with IV PEG than with IM E. coli ASP (Table 1). An NSAA of ≥ 0.1 IU/mL was achieved in ≥ 95% of IV PEG pts compared with < 50% of IM E. coli ASP pts (p<0.01 at each timepoint). There was no significant difference in ASP-related toxicities (allergy, pancreatitis, thrombosis) between the two types of ASP (Table 2). Older pts (≥ 10 yrs old) had a significantly higher overall rate (p<0.01) of pancreatitis (18% vs 7%) and thrombosis (18% vs 4%), but not of allergy (p=0.49) or infection (p=0.21), compared to younger pts. There was no significant difference in the rates of ASP-related toxicities when comparing IM E. coli ASP vs IV PEG separately within the two age groups (≥10 yrs and < 10 yrs). We conclude that every 2-week IV PEG is no more toxic than weekly IM E. coli ASP in children and adolescents with ALL, and is associated with higher serum ASP activity. Longer follow-up is necessary to determine whether there is any difference in event-free survival between the two treatment arms.Table 1:Nadir Serum ASP activity (NSAA) during 30-week Consolidation phaseIV PEGIM ECOLISample Time (wks)*NMedian IU/mL% pts with NSAA ≧ 0.10 IU/mLNMedian IU/mL% pts with NSAA ≧ 0.10 IU/mL5840.6795%920.09448%11700.7197%740.09447%17730.7697%860.09247%23600.70100%760.09446%29680.70100%630.09544%*Number of weeks after start of Consolidation phaseTable 2:Toxicities by ASP type during 30-week Consolidation phaseToxicityIV PEG # of pts (%)IM E. COLI # of pts (%)p-valueNumber of Patients232231Asparaginase Toxicity59 (25)58 (25)>0.99    Allergy26 (11)20 (9)0.44    Pancreatitis25 (11)21 (9)0.64        Mild/Moderate13 (6)13 (6)        Severe12 (5)8 (3)    Thrombosis14 (6)21 (9)0.22Infection (bacteremia, invasive fungal disease)35 (15)46 (20)0.18 Disclosures: Silverman: Enzon Pharmaceuticals: Honoraria. Supko:Enzon Pharmaceuticals: Research Funding. Sallan:Enzon Pharmaceuticals: Honoraria.

Age-related lymphoma patient experience: Results from the Lymphoma Coalition 2018 Global Patient Survey.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e20071-e20071
Author(s):  
Raul Cordoba ◽  
Natacha Bolanos ◽  
Lorna Warwick ◽  
Natalie Dren
Keyword(s):  
Patient Experience ◽  
Age Groups ◽  
Well Being ◽  
Patient Survey ◽  
Activity Level ◽  
Age Group ◽  
Lymphoma Patient ◽  
Medical Issues ◽  
Age Related ◽  
Patient Reported

e20071 Background: Over the past two decades, the incidence of lymphoma has increased by 8-10% per year in older adults. Despite the growing population of older cancer patients, there are a limited number of studies that focus on the experience of these patients. Lymphoma Coalition (LC) saw the need in the 2019 Report Card on Lymphomas to examine the age-related lymphoma patient experience. The objectives of this study were to identify: 1) informational needs and understanding levels, 2) physical conditions and medical issues, 3) psychosocial issues, and 4) barriers in patient-doctor communication. Methods: Using a subset of data from the LC 2018 Global Patient Survey on Lymphomas and CLL (LC 2018 GPS), this study will specifically examine the experiences of newly diagnosed lymphoma patients within the following age categories: 1) Young (18-59) (n = 1473); Mid (60-69) (n = 371); Old (70+) (n = 162). Results: From January to March 2018, 2,006 participants were included in this analysis from 47 countries. The majority of all three patient groups wanted additional medical information beyond what was provided at their diagnosis meeting with the doctor, with the greatest need for information in the young patient group (73%). The highest proportion of poor understanding was reported by the mid-age group (14%) and the highest proportion of very good understanding was reported by the old age group (53%). Across all three age groups, fatigue was the top reported physical condition affecting well-being. Regarding older patients, hair loss was reported in 36% vs 54% in younger patients (p = 0.01), and muscle weakness was reported in 40%. A greater proportion of those in the old group reported that their lifestyle (86%) and general activity level (87%) had been affected. The reported prevalence of medical issues was highest in the young group and lowest in the old group, both during and after treatment. Fear of relapse was the top reported psychosocial issue following treatment for patients in all three age groups. Of those who discussed their fear of relapse with their doctor (young 42% vs old 21%, p = 0.0022), less than one third of patients felt their discussion helped alleviate the fear. Conclusions: This study emphasizes that lymphoma patients in all age groups need more information and support beyond what is currently being provided. While certain age-specific trends were identified, the majority of patient-reported issues span across all three of the age groups examined.

Physical Activity in the EPIC-Italy Centers

2003 ◽  
Vol 89 (6) ◽  
pp. 646-655 ◽  
Author(s):  
Simonetta Salvini ◽  
Calogero Saieva ◽  
Anna Vittoria Ciardullo ◽  
Salvatore Panico ◽  
Giovanna Masala ◽  
...  
Keyword(s):  
Physical Activity ◽  
Leisure Time ◽  
Activity Index ◽  
Age Groups ◽  
Physical Activity Promotion ◽  
Activity Level ◽  
Activity Promotion ◽  
Energy Consuming ◽  
Intense Activity ◽  
Local Traditions

The European Prospective Investigation into Cancer and nutrition offers the opportunity to explore patterns of physical activity in a large series of healthy adults enrolled in the different local cohorts of the Italian section of the European EPIC project. Physical activity is considered one of the means by which chronic disease could be prevented. Subjects in the EPIC study completed a life-style questionnaire, with a section dedicated to the assessment of physical activity at work and during leisure time. Time spent in the various activities was transformed into an index of physical activity (physical activity level, PAL) and an activity index that includes intense activity (PAL; intense activity included). Quintiles of these indexes were computed in order to observe the distribution of subject characteristics according to levels of physical activity. In general, the population was characterized by low levels of physical activity at work, with more than 50% of the sample reporting sedentary occupations. During leisure time, only a small percentage of subjects compensated for the inactivity at work by engaging in energy-consuming activities. In particular, organized fitness activities were reported by a small percentage of people, whereas walking was the most common sort of physical activity. Specific types of activity seemed to characterize subjects in the different areas of the country, reflecting local traditions or specific living situations. Detailed information about physical activity habits, together with a description of other characteristics, could help in designing physical activity promotion programs in different Italian populations and age groups.

scholarly journals Highly Variable Genomic Landscape of Endogenous Retroviruses in the C57BL/6J Inbred Strain, Depending on Individual Mouse, Gender, Organ Type, and Organ Location

2017 ◽  
Vol 2017 ◽  
pp. 1-10
Author(s):  
Kang-Hoon Lee ◽  
Debora Lim ◽  
David Greenhalgh ◽  
Kiho Cho
Keyword(s):  
Germ Line ◽  
Inbred Mice ◽  
Leukemia Virus ◽  
Age Groups ◽  
Endogenous Retroviruses ◽  
Tissue Cell ◽  
Genomic Landscape ◽  
Individual Mouse ◽  
Spatial Diversification ◽  
The Individual

Transposable repetitive elements, named the “TREome,” represent ~40% of the mouse genome. We postulate that the germ line genome undergoes temporal and spatial diversification into somatic genomes in conjunction with the TREome activity. C57BL/6J inbred mice were subjected to genomic landscape analyses using a TREome probe from murine leukemia virus-type endogenous retroviruses (MLV-ERVs). None shared the same MLV-ERV landscape within each comparison group: (1) sperm and 18 tissues from one mouse, (2) six brain compartments from two females, (3) spleen and thymus samples from four age groups, (4) three spatial tissue sets from two females, and (5) kidney and liver samples from three females and three males. Interestingly, males had more genomic MLV-ERV copies than females; moreover, only in the males, the kidneys had higher MLV-ERV copies than the livers. Perhaps, the mouse-, gender-, and tissue/cell-dependent MLV-ERV landscapes are linked to the individual-specific and dynamic phenotypes of the C57BL/6J inbred population.

scholarly journals Oxygen-dependence of ACTH-stimulated aldosterone and corticosterone synthesis in the rat adrenal cortex: developmental aspects

2002 ◽  
Vol 172 (3) ◽  
pp. 595-604 ◽  
Author(s):  
ED Bruder ◽  
AK Nagler ◽  
H Raff
Keyword(s):  
Enzyme Activity ◽  
Age Groups ◽  
Maximal Activity ◽  
Zona Glomerulosa ◽  
Zona Fasciculata ◽  
Steroid Synthesis ◽  
Camp Generation ◽  
Aldosterone Production ◽  
Health And Disease

The control of ACTH-stimulated steroidogenesis under decreasing levels of O(2) is not fully understood. The purpose of this study was to examine the effects of decreased O(2) in vitro on rat adrenocortical steroid synthesis at different stages of development. Of interest was the evaluation of the effect of low O(2) on steroidogenesis during the stress hyporesponsive period of the neonate. Rats were killed at 7, 14, or 42 days of age, adrenals collected and capsules (zona glomerulosa, ZG) separated from subcapsules (zona fasciculata/reticularis, ZFR). Cells were dispersed and placed into glass vials each gassed with a different level of O(2) (21, 5, 2, 1, or 0% O(2)). The entire steroidogenic pathway was analyzed by measuring ACTH-stimulated cAMP, corticosterone and aldosterone production during a 2 h incubation. In addition, the early (P450 scc) and late (P450c11 beta and P450 aldo) pathway activities were examined in the presence of cyanoketone. The PO(2) for half-maximal activity (P(50)) for aldosterone synthesis in ZG cells from 7- and 42-day-old rats was approximately 28 mmHg and 7 mmHg respectively, indicating that cells from older rats were more resistant to inhibition by low O(2). The P(50) for cAMP production from the ZG was approximately 14 mmHg for both age groups. The P(50) for corticosterone synthesis was approximately 28 mmHg and <7 mmHg in ZFR cells from 7- and 42-day-old cells respectively. The only enzyme activities affected by low O(2) (<35 mmHg) were P450 aldo and P450 scc. Moderate decreases in O(2) (from approximately 150 mmHg) decreased aldosteronogenesis, possibly due to observed decreases in cAMP generation, but not due to decreases in steroidogenic enzyme activity (7-day-old). Severe decreases in O(2) presumably inhibited P450 aldo through a direct effect on enzyme activity (both ages). P450 scc activity (including cholesterol transport) also seems to be decreased by very low O(2) (7-day-old). These findings illustrate a novel developmental alteration in O(2)-regulated steroid production, and may have implications for neonatal health and disease.

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