Experimental comparison of restricted selection index and restricted independent culling levels in Tribolium castaneum

Genome ◽  
1987 ◽  
Vol 29 (1) ◽  
pp. 91-96 ◽  
Author(s):  
J. L. Campo ◽  
B. Villanueva
Keyword(s):  
Tribolium Castaneum ◽  
Selection Index ◽  
Egg Laying ◽  
Experimental Comparison ◽  
Pupal Weight ◽  
Selection Methods ◽  
Adult Weight ◽  
Antagonistic Selection ◽  
Independent Culling ◽  
Restricted Selection

A selection index (I) method was compared with independent culling levels (N), with a restriction in the selection program, using two replicated single generation experiments in Tribolium castaneum, which are considered forms of antagonistic selection. The first experiment was designed to increase adult weight without changing pupal weight, while the second experiment was intended to improve egg laying without changing adult weight. In both experiments the genetic correlation between the traits involved were positive but were higher in experiment 1 than in experiment 2. The proportion of selection was 10%. In experiment 1, the effect of restriction was as expected in both lines since the changes in pupal weight were not significant. Adult weight change was positive for the I line and negative for the N line, showing that the index was a superior method to improve adult weight. In experiment 2, the effect of restriction was also as expected in both lines and changes in adult weight were not significant. Egg laying changed positively in both lines. Therefore, both selection methods were similar in this experiment, even though egg laying change was higher in the I than the N line. Key words: restricted index, restricted culling levels, antagonistic selection, Tribolium.

Realized genetic parameters from an antagonistic selection index in Tribolium castaneum

1986 ◽  
Vol 28 (3) ◽  
pp. 358-364 ◽  
Author(s):  
J. L. Campo ◽  
L. G. Raya
Keyword(s):  
Genetic Correlation ◽  
Tribolium Castaneum ◽  
Selection Index ◽  
Selection Response ◽  
Pupal Weight ◽  
Base Population ◽  
Adult Weight ◽  
Antagonistic Selection ◽  
Individual Trait ◽  
Highly Correlated

Simultaneous selection of two highly correlated traits was practiced in Tribolium castaneum in a replicated experiment for six generations. Two lines were selected in each of three replicates: P+A− for an antagonistic selection index to increase pupal weight at 21 days and decrease adult weight at 31 days, and P−A+ for an antagonistic selection index to decrease pupal weight and increase adult weight. Genetic correlation between pupal and adult weights estimated in the base population was 0.95. Estimates of realized heritabilities and genetic correlation were obtained and compared with full-sib estimates. For the aggregate genotype, the P+A− line had a significant positive selection response, while the P−A+ line showed a significant negative response as would be expected. The response to divergent selection was asymmetrical, being less for the low pupal weight and high adult weight line than vice versa. Less asymmetry was found for the realized heritability, which agreed very well with the index heritability estimated in the base population. Both individual traits changed in the same direction, increasing for the P+A− line and decreasing for P−A+ line, even though a significant response was found only for pupal weight in the P+A− line. Therefore, the response to antagonistic selection for each individual trait seems to be heavily controlled by the strength of the genetic correlation between them. The realized genetic correlation between pupal weight and adult weight obtained from antagonistic selection index was in excellent agreement with full-sib estimates. Realized heritabilities for each trait were overestimated, especially for adult weight, even though there were essentially no differences between realized and estimated values.Key words: selection (artificial), antagonistic index, parameters (realized), Tribolium.

COMPONENTS OF GROWTH IN GENETICALLY DIVERSE POPULATIONS OF TRIBOLIUM CASTANEUM

1969 ◽  
Vol 11 (4) ◽  
pp. 896-907 ◽  
Author(s):  
DuWayne C. Englert ◽  
A. E. Bell
Keyword(s):  
Tribolium Castaneum ◽  
Adult Emergence ◽  
Pupal Weight ◽  
Diverse Populations ◽  
Correlated Responses ◽  
Larval Weight ◽  
Developmental Patterns ◽  
Adult Weight ◽  
Total Growth ◽  
Selection For

The growth and developmental patterns of 19 genetically diverse populations of Tribolium castaneum were examined. Measurements were obtained on three primary traits (13-day larval weight, pupation time and pupal weight) and three secondary traits (number of larval molts, adult emergence time and adult weight). The populations were chosen on the basis of their previous selection histories. Selection for different primary traits produced profound effects upon the total growth and development complex.Genetic and environmental relationships between primary traits were estimated within each population, and a consistent asymmetry of correlated responses was observed to be determined by the direction of selection.Possible effects upon the disruption of an intricate balance of the three metamorphosis hormones (activation, molting and juvenile) through selection were postulated.

An experimental comparison of selection methods

Genome ◽  
1989 ◽  
Vol 32 (5) ◽  
pp. 791-795
Author(s):  
D. C. Winkelman ◽  
I. McMillan ◽  
G. W. Friars ◽  
L. R. McKay
Keyword(s):  
Response Selection ◽  
Experimental Comparison ◽  
Pupal Weight ◽  
Control Line ◽  
Response To Selection ◽  
Selection Methods ◽  
Single Generation ◽  
Sire Family ◽  
Males And Females ◽  
Selection For

Five methods of selection each in five replicates were examined for single-generation response in high and low 21-day pupal weight of Tribolium castaneum. The methods of selection tested were individual (ID), dam family or full (DF), sire family or half-sib (SF), progeny test (PT), and index (IX). A selection intensity, involving as parents the top 25% of the individuals on any specific phenotypic scale, was applied throughout the experiment. Each replicate included a contemporaneous, randomly mated control line. Responses attained with each method were compared with predicted gains and with results observed for other methods. Differences in responses of males and females were also assessed. Response to selection was markedly asymmetrical with greater gains observed in the downward direction. In the lines selected for low pupal weight, males and females were found to respond similarly and actual responses did not differ from those predicted. Ranking of methods by responses obtained for downward selection yielded the order IX > ID > PT > DF > SF. Selection for high pupal weight yielded differences in responses of males and females and the observed responses were significantly less than those predicted in all cases except that of PT-selected females. Observed responses for males were ordered as IX > ID > PT > DF > SF, while those for females were PT > IX > DF > SF > ID. When the data from both sexes were combined, the order of responses was PT > IX > DF > ID > SF.Key words: asymmetrical response, selection, Tribolium, pupal weight.

Latrophilin mediates insecticides susceptibility and fecundity through two carboxylesterases, esterase4 and esterase6, in Tribolium castaneum

2019 ◽  
Vol 109 (4) ◽  
pp. 534-543 ◽  
Author(s):  
L. Wei ◽  
S. Gao ◽  
W. Xiong ◽  
J. Liu ◽  
J. Mao ◽  
...  
Keyword(s):  
Tribolium Castaneum ◽  
Molecular Mechanisms ◽  
Vital Role ◽  
Egg Laying ◽  
Egg Hatching ◽  
Compensatory Response ◽  
Physiological Processes ◽  
Similar Phenotype ◽  
G Protein Coupled ◽  
Insight Into

AbstractLatrophilin (LPH) is known as an adhesion G-protein-coupled receptor which involved in multiple physiological processes in organisms. Previous studies showed that lph not only involved the susceptibility to anticholinesterase insecticides but also affected fecundity in Tribolium castaneum. However, its regulatory mechanisms in these biological processes are still not clear. Here, we identified two potential downstream carboxylesterase (cce) genes of Tclph, esterase4 and esterase6, and further characterized their interactions with Tclph. After treatment of T. castaneum larvae with carbofuran or dichlorvos insecticides, the transcript levels of Tcest4 and Tcest6 were significantly induced from 12 to 72 h. RNAi against Tcest4 or Tcest6 led to the higher mortality compared with the controls after the insecticides treatment, suggesting that these two genes play a vital role in detoxification of insecticides in T. castaneum. Furthermore, with insecticides exposure to Tclph knockdown beetles, the expression of Tcest4 was upregulated but Tcest6 was downregulated, indicating that beetles existed a compensatory response against the insecticides. Additionally, RNAi of Tcest6 resulted in 43% reductions in female egg laying and completely inhibited egg hatching, which showed the similar phenotype as that of Tclph knockdown. These results indicated that Tclph affected fecundity by positively regulating Tcest6 expression. Our findings will provide a new insight into the molecular mechanisms of Tclph involved in physiological functions in T. castaneum.

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