Mise en évidence d'une liaison génétique entre un gène de nanisme et des marqueurs enzymatiques chez le mil pénicillaire (Pennisetum glaucum L.)

1985 ◽  
Vol 27 (6) ◽  
pp. 751-758 ◽  
Author(s):  
Serge Tostain
Keyword(s):  
Discriminant Analysis ◽  
Alcohol Dehydrogenase ◽  
Genetic Linkage ◽  
Pennisetum Glaucum ◽  
Inbred Lines ◽  
Marker Genes ◽  
Linkage Groups ◽  
Shikimate Dehydrogenase ◽  
Dwarfing Gene ◽  
Enzymic Marker

The genetic linkage relations between the dwarfing (D2, d2) and seven enzymic marker genes were evaluated in three crosses between semidwarf and normal inbred lines of pearl millet (Pennisetum glaucum L.). The seven genes code for the following isoenzymes: alcohol dehydrogenase (Adh A), esterase (Est A), malate dehydrogenase (Mdh D) cathodic peroxydase (Pec A), phosphoglucoisomerase (Pgi A), phospholucomutase (Pgm A), and shikimate dehydrogenase (Skdh A). Semidwarf and normal plants were identified by a discriminant analysis based on 16 morphological height components. Mendelian segregations have been observed for all eight genes. Linkage was shown between Pgi A and Pgm A (4 ± 4 centimorgans (cM)), between Skdh A and Adh A (11 ± 7 cM), between D2 and Skdh A (9 ± 5 cM) and between D2 and Adh A (17 ± 8 cM). The latter three genes are linked in the following order: Adh A – 11 cM – Skdh A – 9 cM – D2. The linkage between the recessive dwarfing gene (d2) and the codominant Skdh A alleles will help in the creation of isogenic semidwarf lines of cultivars. It is highly probable that the D2d2 genotype could be separated from the D2D2 genotype.Key words: isozymes, linkage groups, early selection.

scholarly journals Construction of a genetic linkage map in Lilium using a RIL mapping population based on SRAP marker

Genetika ◽  
2015 ◽  
Vol 47 (2) ◽  
pp. 425-438 ◽  
Author(s):  
Li-Jing Chen ◽  
Hong-Mei Li ◽  
Shao-Kun Sun ◽  
Muhammad Irfan ◽  
Jing-Wei Lin ◽  
...  
Keyword(s):  
Linkage Map ◽  
Recombinant Inbred ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Mapping Population ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Linkage Groups ◽  
Marker Interval ◽  
Interval Distance

A genetic linkage map of lily was constructed using RILs (recombinant inbred lines) population of 180 individuals. This mapping population was developed by crossing Raizan No.1 (Formolongo) and Gelria (Longiflomm) cultivars through single-seed descent (SSD). SRAPs were generated by using restriction enzymes EcoRI in combination with either MseI. The resulting products were separated by electrophoresis on 6% denaturing polyacrylamide gel and visualized by silver staining. The segregation of each marker and linkage analysis was done using the program Mapmaker3.0. With 50 primer pairs, a total of 189 parental polymorphic bands were detected and 78 were used for mapping. The total map length was 2,135.5 cM consisted of 16 linkage groups. The number of markers in the linkage groups varied from 1 to 12. The length of linkage groups was range from 11.2 cM to 425.9 cM and mean marker interval distance range from 9.4 cM to 345.4 cM individually. The mean marker interval distance between markers was 27.4 cM. The map developed in the present study was the first sequence-related amplified polymorphism markers map of lily constructed with recombinant inbred lines, it could be used for genetic mapping and molecular marker assisted breeding and quantitative trait locus mapping of Lilium.

scholarly journals Secondary Trisomics and Telotrisomics of Rice: Origin, Characterization, and Use in Determining the Orientation of Chromosome Map

Genetics ◽  
1996 ◽  
Vol 143 (1) ◽  
pp. 517-529
Author(s):  
Kuldeep Singh ◽  
D S Multani ◽  
Gurdev S Khush
Keyword(s):  
Linkage Map ◽  
Large Population ◽  
Marker Genes ◽  
Linkage Groups ◽  
Breeding Behavior ◽  
Specific Chromosome ◽  
Chromosome Map ◽  
Primary Trisomics ◽  
Correct Orientation ◽  
Genetic Segregation

Abstract Secondary trisomics and telotrisomics representing the 12 chromosomes of rice were isolated from the progenies of primary trisomics. A large population of each primary trisomic was grown. Plants showing variation in gross morphology compared to the primary trisomics and disomic sibs were selected and analyzed cytologically at diakinesis and pachytene. Secondary trisomics for both arms of chromosomes 1, 2, 6, 7 and 11 and for one arm of chromosomes 4, 5, 8, 9 and 12 were identified. Telotrisomics for short arm of chromosomes 1, 8, 9 and 10 and for long arms of chromosomes 2, 3 and 5 were isolated. These secondary and telotrisomics were characterized morphologically and for breeding behavior. Secondary trisomics 2n + 1S · 1S, 2n + 1L · 1L, 2n + 2S · 2S, 2n + 2L · 2L, 2n + 6S · 6S, 2n + 6L · 6L and 2n + 7L · 7L are highly sterile, and 2n + 1L · 1L, 2n + 2L · 2L and 2n + 7L · 7L do not set any seed even upon backcrossing. Telotrisomics are fertile and vigorous. Genetic segregation of 43 marker genes was studied in the F2 or backcross progenies. On the basis of segregation data, these genes were delimited to specific chromosome arms. Correct orientation of 10 linkage groups was determined and centromere positions on nine linkage groups were approximated. A revised linkage map of rice is presented.

Genetic Linkage Maps of the Guppy ( Poecilia reticulata ): Assignment of RAPD Markers to Multipoint Linkage Groups

2003 ◽  
Vol 5 (3) ◽  
pp. 279-293 ◽  
Author(s):  
Gideon Khoo ◽  
Meng Huat Lim ◽  
Haridas Suresh ◽  
Damien K. Y. Gan ◽  
Kok Fang Lim ◽  
...  
Keyword(s):  
Genetic Linkage ◽  
Rapd Markers ◽  
Poecilia Reticulata ◽  
Linkage Maps ◽  
Linkage Groups ◽  
Multipoint Linkage ◽  
Genetic Linkage Maps

Gene-based anchoring of the rat genetic linkage and cytogenetic maps: new regional localizations, orientation of the linkage groups, and insights into mammalian chromosome evolution

1998 ◽  
Vol 9 (9) ◽  
pp. 721-734 ◽  
Author(s):  
Claude Szpirer ◽  
Josiane Szpirer ◽  
Pascale Van Vooren ◽  
Fadel Tissir ◽  
Jason S. Simon ◽  
...  
Keyword(s):  
Genetic Linkage ◽  
Chromosome Evolution ◽  
Linkage Groups

scholarly journals Application of multivariate techniques in the evaluation of pure lines of beans

2016 ◽  
Vol 46 (9) ◽  
pp. 1535-1541 ◽  
Author(s):  
Rodolfo Schmit ◽  
Rita Carolina de Melo ◽  
Thayse Cristine Vieira Pereira ◽  
Mattheus Beck ◽  
Altamir Frederico Guidolin ◽  
...  
Keyword(s):  
Discriminant Analysis ◽  
Discriminant Function ◽  
Block Design ◽  
Original Data ◽  
Inbred Lines ◽  
Canonical Discriminant Analysis ◽  
Multivariate Techniques ◽  
Testing Hypotheses ◽  
Randomized Block Design ◽  
Pure Lines

ABSTRACT: The objective of this study was to apply multivariate techniques, canonical discriminant analysis, and multivariate contrasts, indicating the most favorable inferences in the evaluation of pure lines of beans. The study was conducted at the experimental field of the Institute for Breeding and Molecular Genetics, in Lages, SC, Brazil. The experiment was composed of 24 pure lines of beans from the Santa Catarina test of cultivars. Plant height, numbers of pods and grains per plant, and stem diameter were the variables measured. The complete randomized block design was used with four replications. The data were subjected to multivariate analysis of variance, canonical discriminant analysis, multivariate contrasts and univariate contrasts. The first canonical discriminant function has captured 81% of the total variation in the data. The Scott-Knott test showed two groups of inbred lines at the average -of scores of the first canonical discriminant function. It was considered that testing hypotheses with the canonical scores may result in loss of information obtained from the original data. Multivariate contrasts indicated differences within the group formed by the Scott-Knott test. The canonical discriminant analysis and multivariate contrasts are excellent techniques to be combined in the multivariate assessment, being used to explore and test hypotheses, respectively.

scholarly journals Genetic linkage groups in the Japanese brown frog (Rana japonica

2000 ◽  
Vol 91 (1) ◽  
pp. 1-7 ◽  
Author(s):  
M. Sumida ◽  
M. Nishioka
Keyword(s):  
Genetic Linkage ◽  
Linkage Groups ◽  
Rana Japonica ◽  
Brown Frog

Two genetic linkage maps of mungbean using RFLP and RAPD markers

2000 ◽  
Vol 51 (4) ◽  
pp. 415 ◽  
Author(s):  
C. J. Lambrides ◽  
R. J. Lawn ◽  
I. D. Godwin ◽  
J. Manners ◽  
B. C. Imrie
Keyword(s):  
Linkage Map ◽  
Segregation Distortion ◽  
Recombinant Inbred ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Rflp Markers ◽  
Linkage Maps ◽  
Linkage Groups ◽  
Genetic Linkage Maps ◽  
Map Distance

Two genetic linkage maps of mungbean derived from the cross Berken ACC 41 are reported. The F2 map constructed from 67 individuals consisted of 110 markers (52 RFLP and 56 RAPD) that grouped into 12 linkage groups. The linked markers spanned a total map distance of 758.3 cM. A recombinant inbred (RI) population derived from the 67 F2 individuals was used for the generation of an additional linkage map. The RI map, composed entirely of RAPD markers, consisted of 115 markers in 12 linkage groups. The linked markers spanned a total map distance of 691.7 cM. Using a framework set of RFLP markers, the F2 map was compared with another F2 mungbean map constructed in Minnesota. In general, the order of these markers was consistent between maps. Segregation distortion was observed for some markers. 14.5% (16/110) of mapped F2 markers and 24% (28/115) of mapped RI markers segregated with distorted ratios. Segregation distortion occurred in each successive generation after the F2 . The regions of distortion identified in the Australian maps did not coincide with regions of the Minnesota map.

A high-resolution genetic linkage map of soybean based on 357 recombinant inbred lines genotyped with BARCSoySNP6K

2015 ◽  
Vol 35 (2) ◽  
Author(s):  
Sungwoo Lee ◽  
Keith R. Freewalt ◽  
Leah K. McHale ◽  
Qijian Song ◽  
Tae-Hwan Jun ◽  
...  
Keyword(s):  
High Resolution ◽  
Linkage Map ◽  
Recombinant Inbred ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Recombinant Inbred Lines ◽  
Inbred Lines

scholarly journals Genetic Linkage Map of the Edible BasidiomycetePleurotus ostreatus

2000 ◽  
Vol 66 (12) ◽  
pp. 5290-5300 ◽  
Author(s):  
Luis M. Larraya ◽  
G�mer P�rez ◽  
Enrique Ritter ◽  
Antonio G. Pisabarro ◽  
Lucı́a Ramı́rez
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Gene Sequence ◽  
Genetic Linkage Map ◽  
Fragment Length Polymorphism ◽  
Individual Cell ◽  
Random Amplified Polymorphic Dna ◽  
Rrna Gene ◽  
Linkage Groups ◽  
Rrna Gene Sequence

ABSTRACT We have constructed a genetic linkage map of the edible basidiomycete Pleurotus ostreatus (var. Florida). The map is based on the segregation of 178 random amplified polymorphic DNA and 23 restriction fragment length polymorphism markers; four hydrophobin, two laccase, and two manganese peroxidase genes; both mating type loci; one isozyme locus (est1); the rRNA gene sequence; and a repetitive DNA sequence in a population of 80 sibling monokaryons. The map identifies 11 linkage groups corresponding to the chromosomes ofP. ostreatus, and it has a total length of 1,000.7 centimorgans (cM) with an average of 35.1 kbp/cM. The map shows a high correlation (0.76) between physical and genetic chromosome sizes. The number of crossovers observed per chromosome per individual cell is 0.89. This map covers nearly the whole genome of P. ostreatus.

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