Chromosome pairing failure in an intersectional amphiploid of Bromus altissimus × B. arvensis

1985 ◽  
Vol 27 (6) ◽  
pp. 705-709 ◽  
Author(s):  
K. C. Armstrong
Keyword(s):  
Chromosome Pairing ◽  
Embryo Culture ◽  
Differential Staining ◽  
Root Tip ◽  
Giemsa Banding ◽  
Homologous Pairing ◽  
Hybrid Nature ◽  
Tip Cells ◽  
Root Tip Cells

An intersectional F1 hybrid between Bromus arvensis and B. altissimus was made with the aid of embryo culture. The hybrid nature of the F1 and the amphiploid were confirmed by karyotyping root-tip cells. Following Giemsa banding, the chromosomes of B. arvensis stained darker than those of B. altissimus. Very little chromosome pairing was observed in the F1 hybrid (11.60 I + 1.08 II + 0.10 III). Chromosome pairing in the amphiploid (2n = 28) varied from almost complete pairing to very little pairing in different samples. The chromosome pairing indicated that very little homology exists between the genomes of B. altissimus and B. arvensis. Pairing failure in the amphiploid may result from the action of pairing control genes which are strong enough to prevent homologous pairing but which are variable in expression because of a sensitivity to endogenous and exogenous factors.Key words: Bromus, amphiploid, chromosome pairing, differential staining.

Diploid perennial intergeneric hybrids in the tribe Triticeae. III. Hybrids among Secale montanum, Pseudoroegneria spicata, and Agropyron mongolicum

Genome ◽  
1987 ◽  
Vol 29 (1) ◽  
pp. 80-84 ◽  
Author(s):  
Richard R.-C. Wang
Keyword(s):  
Key Words ◽  
Embryo Culture ◽  
Intergeneric Hybrids ◽  
Root Tip ◽  
Pseudoroegneria Spicata ◽  
Chromosome Homology ◽  
Tip Cells ◽  
Root Tip Cells ◽  
Secale Montanum ◽  
Tribe Triticeae

Four new intergeneric diploid hybrids were synthesized in the greenhouse with the aid of embryo culture. Hybrids of Pseudoroegneria spicata × Secale montanum and P. spicata ssp. inermis × S. montanum, both having the genome formula SR, averaged 12.97 I + 0.49 II + 0.01 III at metaphase I. The hybrids of Agropyron mongolicum × S. montanum, which have the PR genomes, had an average of 12.86 I + 0.51 II + 0.03 III + 0.004 IV. The hybrid (SP) between P. spicata ssp. inermis and Agropyron mongolicum had a mean configuration of 8.05 I + 2.86 II + 0.07 III + 0.01 IV. All hybrids had intermediate spike morphology, respective to their parents, and were sterile. Mitotic preparations of root-tip cells of these hybrids suggested that the chromosomes of different genomes were spatially separated. The meiotic pairings of these hybrids indicated that chromosome homology between the S and P genomes is higher than either the S and R or the P and R. Both SR and PR hybrids represent new genomic combinations. The SP hybrid is equivalent to the dihaploid of P. tauri. All of these hybrids should be of value for breeding and taxonomy. Key words: intergeneric hybrids, genome, Secale, Pseudoroegneria, Agropyron.

Sunlight decreased genotoxicity of azadirachtin on root tip cells of Allium cepa and Eucrosia bicolor

2010 ◽  
Vol 73 (5) ◽  
pp. 949-954 ◽  
Author(s):  
W. Kwankua ◽  
S. Sengsai ◽  
C. Kuleung ◽  
N. Euawong
Keyword(s):  
Allium Cepa ◽  
Root Tip ◽  
Tip Cells ◽  
Root Tip Cells

Salt Stress-induced Programmed Cell Death in Rice Root Tip Cells

2007 ◽  
Vol 49 (4) ◽  
pp. 481-486 ◽  
Author(s):  
Jian-You Li ◽  
Ai-Liang Jiang ◽  
Wei Zhang
Keyword(s):  
Cell Death ◽  
Salt Stress ◽  
Programmed Cell Death ◽  
Rice Root ◽  
Root Tip ◽  
Tip Cells ◽  
Root Tip Cells

Cytological evidence bearing on the origin of the B genome in polyploid wheats

Genome ◽  
1988 ◽  
Vol 30 (1) ◽  
pp. 36-43 ◽  
Author(s):  
K. Kerby ◽  
J. Kuspira
Keyword(s):  
Dna Hybridization ◽  
Triticum Turgidum ◽  
Root Tip ◽  
Cytological Evidence ◽  
B Genome ◽  
Genome Donor ◽  
The One ◽  
Genome Donors ◽  
Tip Cells ◽  
Root Tip Cells

To help elucidate the origin of the B genome in polyploid wheats, karyotypes of Triticum turgidum, Triticum monoccum, and all six purported B genome donors were compared. The analysis utilized a common cytological procedure that employed the most advanced equipment for the measurement of chromosome lengths at metaphase in root tip cells. A comparison of the karyotypes of T. turgidum and T. monococcum permitted the identification of B genome chromosomes of T. turgidum. These consist of two SAT pairs, one ST pair, three SM pairs, and one M pair of homologues. Comparisons of the chromosomes of the B genome of T. turgidum with the karyotypes of the six putative B genome donors showed that only the karyotype of Aegilops searsii was similar to the one deduced for the donor of the B genome in T. turgidum, suggesting that Ae. searsii is, therefore, the most likely donor of the B genome to the polyploid wheats. Support for this conclusion has been derived from geographic, DNA-hybridization, karyotype, morphological, and protein data reported since 1977. Reasons why the B genome donor has not been unequivocally identified are discussed.Key words: phylogeny, karyotypes, Triticum turgidum, Triticum monococcum, B genome, B genome donors.

scholarly journals Squash Preparations of Living Root-Tip Cells

Nature ◽  
1949 ◽  
Vol 164 (4178) ◽  
pp. 930-930 ◽  
Author(s):  
J. CHAYEN
Keyword(s):  
Root Tip ◽  
Living Root ◽  
Tip Cells ◽  
Root Tip Cells

The effect of cytochalasin D on preprophase band organization in root tip cells of Allium

1992 ◽  
Vol 103 (4) ◽  
pp. 989-998 ◽  
Author(s):  
E.P. Eleftheriou ◽  
B.A. Palevitz
Keyword(s):  
Preprophase Band ◽  
Cytochalasin D ◽  
Root Tip ◽  
Average Width ◽  
Exact Position ◽  
The Relationship ◽  
Tip Cells ◽  
Root Tip Cells ◽  
Control Samples

The relationship between microfilaments (Mfs) and microtubules (Mts) in the organization of the preprophase band (PPB) was investigated in Allium root tip cells subjected to treatment with cytochalasin D (CD). Mts and Mfs were visualized by indirect immunofluorescence and various parameters such as PPB width were analyzed quantitatively. In control samples, the PPB first appears as a wide Mt band that progressively narrows to an average width of 4 micrometre in mid-prophase. Randomly oriented Mfs are present throughout the cytoplasm of most interphase control cells. Preprophase and prophase cells, however, contain cortical Mfs arranged parallel to the PPB. The Mfs initially occupy much of the cortex but in most cells they progressively become restricted to an area wider than the PPB. In the presence of CD, the PPB fails to narrow and remains at least two-fold wider than in control cells. PPB width expressed as a percentage of nuclear or cell length also increases compared to controls. Widening is concentration dependent, and the effect of 10 micromolar CD is near maximal only 15 min after application of the drug. This rapid response suggests that a rebroadening of already condensed PPBs takes place. After as little as 15 min in CD, Mfs are replaced by many small specks and rods. Dual localizations of both Mts and Mfs show that prophase cells contain broad PPBs without Mfs. The rapid disorganization of Mfs, by CD, therefore coincides with the rebroadening of PPBs. CD-treated cells in metaphase, anaphase and telophase contain larger actin aggregates at the poles, as previously reported. The results indicate that Mfs play an important role in the narrowing of the PPB, which in turn is essential for determination of the exact position of the plane of division. They also indicate that movement of intact Mts is important in PPB organization.

Sign in / Sign up

Export Citation Format

Share Document