Highly repeated DNA and holocentric chromosomes of the woodrush Luzula flaccida (Juncaceae)

1984 ◽  
Vol 26 (3) ◽  
pp. 288-295 ◽  
Author(s):  
Chris Collet
Keyword(s):  
Satellite Dna ◽  
Unit Length ◽  
Buoyant Density ◽  
Holocentric Chromosomes ◽  
Repeated Dna ◽  
Base Pairs ◽  
Highly Repeated Dna ◽  
Minor Satellite ◽  
Total Dna ◽  
A Minor

Three highly repeated DNA components were characterized from Luzula flaccida. The 1.683 g/cm3 satellite accounts for 22% of the genome and has a sequence complexity of 127 base pairs (BP). A further 8% of the genome consists of a cryptic satellite DNA with a repeating unit of 190 BP. A minor satellite component of buoyant density 1.681 g/cm3 constitutes less than 2% of the total DNA and has a unit length of 184 BP. The presence of segments of the 1.683 satellite DNA lacking Mbol cleavage sites suggests that amplification of sequence variants may have occurred during the evolution of this satellite. While the distribution of satellite DNA appears to be different in being interspersed along the holocentric chromosomes of L. flaccida, the structure and mode of evolution of highly repeated DNA appears to follow the same pattern as seen in organisms with localized centromeres.Key words: Luzula, holocentry, highly repeated DNA.

scholarly journals A Highly Repeated DNA From the Genome of the Wallaroo (Macro pus Robustus Robustus)

1981 ◽  
Vol 34 (1) ◽  
pp. 97 ◽  
Author(s):  
L Venolia ◽  
WJ Peacock
Keyword(s):  
Dna Sequence ◽  
Repeated Dna ◽  
Chromosome 5 ◽  
Major Satellite ◽  
Sequence Heterogeneity ◽  
Highly Repeated Dna ◽  
Group A ◽  
Total Dna

The major satellite of M. r. robustus DNA has been isolated in a Ag+ -CS2S04 gradient.has a of 1� 710 gjcm3 compared with 1� 697 gjcm3 for the bulk of the DNA, and accounts for about 10% of the total DNA. Sequence heterogeneity within the satellite was shown by an increase in density to 1�715 gjcm3 and by a reduction of 12�C in the temperature of denaturation (Tm) after renaturation. The satellite was found to occur in the centromeric regions of all chromosomes. This pattern of distribution was essentially duplicated in the genomes of other members of the wallaroo group. A polymorphism for a major block of the sequences on chromosome 5 occurred in both M. r. erubescens and M. antilopinus.

scholarly journals Repeated DNA Sequences and Kangaroo Phylogeny

1981 ◽  
Vol 34 (3) ◽  
pp. 325 ◽  
Author(s):  
WJ Peacock ◽  
E S Dennis ◽  
A Elizur ◽  
JH Calaby
Keyword(s):  
Dna Sequences ◽  
Buoyant Density ◽  
Repeated Dna ◽  
Repeated Dna Sequences ◽  
The Third ◽  
Red Kangaroo ◽  
Highly Repeated Dna

Three highly repeated DNA sequences have been used to determine relationships of species within the Macropodidae (kangaroos and wallabies). Two highly repeated DNA sequences were isolated as buoyant density satellites in the red-necked wallaby and in the wallaroo-euro group. The third probe was a cloned representative of one class of highly repeated species from the red kangaroo.

Highly repeated DNA sequences in birds: The structure and evolution of an abundant, tandemly repeated 190-bp DNA fragment in parrots

Genomics ◽  
1992 ◽  
Vol 14 (2) ◽  
pp. 462-469 ◽  
Author(s):  
Cort S. Madsen ◽  
Dineke H. de Kloet ◽  
Jean E. Brooks ◽  
Siwo R. de Kloet
Keyword(s):  
Dna Sequences ◽  
Repeated Dna ◽  
Repeated Dna Sequences ◽  
Highly Repeated Dna ◽  
Dna Fragment

scholarly journals Highly repeated DNA families in the rat.

1984 ◽  
Vol 259 (16) ◽  
pp. 10481-10492
Author(s):  
F R Witney ◽  
A V Furano
Keyword(s):  
Repeated Dna ◽  
Highly Repeated Dna ◽  
Repeated Dna Families

scholarly journals Ultrastructural and Biophysical Characterization of Hepatitis C Virus Particles Produced in Cell Culture

2010 ◽  
Vol 84 (21) ◽  
pp. 10999-11009 ◽  
Author(s):  
Pablo Gastaminza ◽  
Kelly A. Dryden ◽  
Bryan Boyd ◽  
Malcolm R. Wood ◽  
Mansun Law ◽  
...  
Keyword(s):  
Cell Culture ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Structural Characteristics ◽  
Buoyant Density ◽  
Hcv Rna ◽  
Membrane Bilayer ◽  
Biophysical Characterization ◽  
Negative Stain ◽  
A Minor

ABSTRACT We analyzed the biochemical and ultrastructural properties of hepatitis C virus (HCV) particles produced in cell culture. Negative-stain electron microscopy revealed that the particles were spherical (∼40- to 75-nm diameter) and pleomorphic and that some of them contain HCV E2 protein and apolipoprotein E on their surfaces. Electron cryomicroscopy revealed two major particle populations of ∼60 and ∼45 nm in diameter. The ∼60-nm particles were characterized by a membrane bilayer (presumably an envelope) that is spatially separated from an internal structure (presumably a capsid), and they were enriched in fractions that displayed a high infectivity-to-HCV RNA ratio. The ∼45-nm particles lacked a membrane bilayer and displayed a higher buoyant density and a lower infectivity-to-HCV RNA ratio. We also observed a minor population of very-low-density, >100-nm-diameter vesicular particles that resemble exosomes. This study provides low-resolution ultrastructural information of particle populations displaying differential biophysical properties and specific infectivity. Correlative analysis of the abundance of the different particle populations with infectivity, HCV RNA, and viral antigens suggests that infectious particles are likely to be present in the large ∼60-nm HCV particle populations displaying a visible bilayer. Our study constitutes an initial approach toward understanding the structural characteristics of infectious HCV particles.

A highly repeated DNA sequence in Arabidopsis thaliana

1986 ◽  
Vol 204 (3) ◽  
pp. 417-423 ◽  
Author(s):  
Jose M. Martinez-Zapater ◽  
Mark A. Estelle ◽  
Chris R. Somerville
Keyword(s):  
Arabidopsis Thaliana ◽  
Dna Sequence ◽  
Repeated Dna ◽  
Highly Repeated Dna ◽  
Repeated Dna Sequence

Dissociation of minor satellite from the centromere in mouse

1994 ◽  
Vol 107 (11) ◽  
pp. 3091-3095
Author(s):  
B.K. Vig ◽  
D. Latour ◽  
J. Frankovich
Keyword(s):  
Satellite Dna ◽  
Hoechst 33258 ◽  
Major Satellite ◽  
Primary Constriction ◽  
Minor Satellite ◽  
Antibody Reaction

The minor satellite DNA of mouse is believed to constitute the centromere. We report that centromeres of some chromosomes in the Cl1D cells of mouse are not associated with this DNA even though the latter is present on these chromosomes. The satellite DNA was detected distally from the centromere and could not be mistaken as a component of the centromere. We also report that the site of the primary constriction may not always coincide with the site of the anti-kinetochore antibody reaction. Whereas the regions containing the major satellite decondense upon treatment with bisbenzimidazole (Hoechst 33258), the sites carrying minor satellite resist decondensing.

Epigenetic control of mammalian centromere protein binding: does DNA methylation have a role?

1996 ◽  
Vol 109 (9) ◽  
pp. 2199-2206
Author(s):  
A.R. Mitchell ◽  
P. Jeppesen ◽  
L. Nicol ◽  
H. Morrison ◽  
D. Kipling
Keyword(s):  
Dna Sequences ◽  
Satellite Dna ◽  
Inbred Mouse ◽  
Inbred Mouse Strain ◽  
Chromosome 1 ◽  
Satellite Sequences ◽  
E Protein ◽  
Minor Satellite ◽  
Internal Block ◽  
Terminal Block

Chromosome 1 of the inbred mouse strain DBA/2 has a polymorphism associated with the minor satellite DNA at its centromere. The more terminal block of satellite DNA sequences on this chromosome acts as the centromere as shown by the binding of CREST ACA serum, anti-CENP-B and anti-CENP-E polyclonal sera. Demethylation of the minor satellite DNA sequences accomplished by growing cells in the presence of the drug 5-aza-2′-deoxycytidine results in a redistribution of the CENP-B protein. This protein now binds to an enlarged area on the more terminal block and in addition it now binds to the more internal block of minor satellite DNA sequences on chromosome 1. The binding of the CENP-E protein does not appear to be affected by demethylation of the minor satellite sequences. We present a model to explain these observations. This model may also indicate the mechanism by which the CENP-B protein recognises specific sites within the arrays of minor satellite DNA on mouse chromosomes.

Characterization of a Highly Repeated DNA Family in Tapetinae Species (Mollusuca Bivalvia: Veneridae).

1998 ◽  
Vol 15 (4) ◽  
pp. 599-605 ◽  
Author(s):  
Marco Passamonti ◽  
Barbara Mantovani ◽  
Valerio Scali
Keyword(s):  
Repeated Dna ◽  
Highly Repeated Dna
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