scholarly journals A Highly Repeated DNA From the Genome of the Wallaroo (Macro pus Robustus Robustus)

1981 ◽  
Vol 34 (1) ◽  
pp. 97 ◽  
Author(s):  
L Venolia ◽  
WJ Peacock

The major satellite of M. r. robustus DNA has been isolated in a Ag+ -CS2S04 gradient.has a of 1� 710 gjcm3 compared with 1� 697 gjcm3 for the bulk of the DNA, and accounts for about 10% of the total DNA. Sequence heterogeneity within the satellite was shown by an increase in density to 1�715 gjcm3 and by a reduction of 12�C in the temperature of denaturation (Tm) after renaturation. The satellite was found to occur in the centromeric regions of all chromosomes. This pattern of distribution was essentially duplicated in the genomes of other members of the wallaroo group. A polymorphism for a major block of the sequences on chromosome 5 occurred in both M. r. erubescens and M. antilopinus.

1986 ◽  
Vol 204 (3) ◽  
pp. 417-423 ◽  
Author(s):  
Jose M. Martinez-Zapater ◽  
Mark A. Estelle ◽  
Chris R. Somerville

1985 ◽  
Vol 13 (6) ◽  
pp. 1965-1975 ◽  
Author(s):  
Ramareddy V. Guntaka ◽  
Siddarame Gowda ◽  
Arepalli S. Rao ◽  
Theodore J. Green

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1237-1243 ◽  
Author(s):  
K. Hirai ◽  
K. Irifune ◽  
R. Tanaka ◽  
H. Morikawa

A highly repeated DNA sequence with a repeat unit of ca. 180 bp was found in genomic DNA HindIII-digests of Raphanus sativus. The repeating units of six isolated, independent clones were sequenced. These units have 177 or 178 bp, are 36% G+C in their DNA base composition, and show 90% sequence homology. The copy number of this 180-bp repeat unit is about 0.5 × 106 per diploid genome. In situ hybridization analysis with the repeating unit as the probe and C-banding analysis indicated that the repeated DNA sequence of R. sativus is closely associated with the major C-heterochromatins in the proximal regions of all 18 chromosomes at mitotic metaphase.Key words: Raphanus sativus, repeated DNA sequence, nucleotide sequence, in situ hybridization, C-banding.


Chromosoma ◽  
1999 ◽  
Vol 108 (7) ◽  
pp. 436-442 ◽  
Author(s):  
M. Mandrioli ◽  
D. Bizzaro ◽  
G. C. Manicardi ◽  
D. Gionghi ◽  
L. Bassoli ◽  
...  

1993 ◽  
Vol 111 (2-3) ◽  
pp. 147-152 ◽  
Author(s):  
T.J. Doran ◽  
A.L.M. Hodgson ◽  
J.K. Davies ◽  
A.J. Radford

1981 ◽  
Vol 78 (7) ◽  
pp. 4490-4494 ◽  
Author(s):  
W. J. Peacock ◽  
E. S. Dennis ◽  
M. M. Rhoades ◽  
A. J. Pryor

1984 ◽  
Vol 26 (3) ◽  
pp. 288-295 ◽  
Author(s):  
Chris Collet

Three highly repeated DNA components were characterized from Luzula flaccida. The 1.683 g/cm3 satellite accounts for 22% of the genome and has a sequence complexity of 127 base pairs (BP). A further 8% of the genome consists of a cryptic satellite DNA with a repeating unit of 190 BP. A minor satellite component of buoyant density 1.681 g/cm3 constitutes less than 2% of the total DNA and has a unit length of 184 BP. The presence of segments of the 1.683 satellite DNA lacking Mbol cleavage sites suggests that amplification of sequence variants may have occurred during the evolution of this satellite. While the distribution of satellite DNA appears to be different in being interspersed along the holocentric chromosomes of L. flaccida, the structure and mode of evolution of highly repeated DNA appears to follow the same pattern as seen in organisms with localized centromeres.Key words: Luzula, holocentry, highly repeated DNA.


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