C-BANDING OF RYE CHROMOSOMES WITH COLD SSC BUFFER

1976 ◽  
Vol 18 (3) ◽  
pp. 491-496 ◽  
Author(s):  
A. E. Limin ◽  
J. Dvořák
Keyword(s):  
Secale Cereale ◽  
Banding Pattern ◽  
Chemical Structure ◽  
Barium Hydroxide ◽  
C Banding ◽  
Dna Reassociation ◽  
Secale Cereale L

Various times, temperatures and concentrations of SSC were tested in an attempt to elucidate the mechanism of C-banding in plants. It is shown that C-bands can be induced in rye (Secale cereale L.) chromosomes by SSC treatment at temperatures as low as 0 °C for periods as short as 1 min, an effect previously unknown in either plants or animals. Barium hydroxide treatment appears to be essential for the production of bands. If chromosomes are treated with SSC omitting the Ba(OH)2 treatment, relatively uniform loss of nucleoproteins occurs without the production of C-bands. It is suggested that Ba(OH)2 alters the chemical structure of nucleoproteins in heterochromatin rendering them insoluble in SSC. It is unlikely that SSC functions as a DNA reassociation agent in the production of C-bands. More likely it functions as a leaching agent which extracts soluble nucleoproteins from the chromosomes. Incubation in 2 × SSC at room temperatures for 5-10 min was found to be sufficient for the production of a well contrasted banding pattern in rye chromosomes.

A TENTATIVE IDENTIFICATION OF CHROMOSOMES PRESENT IN TETRAPLOID TRITICALE BASED ON HETEROCHROMATIC BANDING PATTERNS

1978 ◽  
Vol 20 (2) ◽  
pp. 199-204 ◽  
Author(s):  
J. P. Gustafson ◽  
K. D. Krolow
Keyword(s):  
Secale Cereale ◽  
Banding Pattern ◽  
Triticum Turgidum ◽  
Staining Technique ◽  
Wheat Genome ◽  
Banding Patterns ◽  
Tentative Identification ◽  
C Banding ◽  
B Genome ◽  
Secale Cereale L

Three tetraploid triticales were analysed by C-banding techniques in order to establish their chromosome constitutions. All three tetraploid triticales contained seven rye chromosomes with the banding pattern of Secale cereale L. A mixture of A- and B-genome chromosomes from Triticum turgidum L. constituted the wheat genome present in the tetraploid triticales. Triticale Trc 4x3 contained 1A, 2B, 3A, 4A, 5B, 6A, and 7B. Triticale Trc 4x2 contained 1A, 2B, 3B, 4B, 5B, 6A, and 7B, while triticale Trc 4x5 contained 1A, 2B, 3B, 4A, 5A, 6A, and 7B. The reliability of the staining technique is subject to errors in identification, which are discussed.

POLYMORPHISM IN THE GIEMSA C-BANDING PATTERN OF RYE CHROMOSOMES

1978 ◽  
Vol 20 (3) ◽  
pp. 307-312 ◽  
Author(s):  
T. Lelley ◽  
K. Josifek ◽  
P. J. Kaltsikes
Keyword(s):  
Secale Cereale ◽  
Banding Pattern ◽  
Inbred Lines ◽  
Giemsa Staining ◽  
C Banding ◽  
Secale Cereale L

Extensive polymorphism was found with regard to the presence and size of Giemsa-staining bands in the chromosomes of six inbred lines of cultivated rye (Secale cereale L.). The amount of polymorphism differed from chromosome to chromosome, with 6R being the most variable and 3R or 7R the least.

Cytological identification of telotrisomic and double ditelosomic lines in Secale cereale cv. Heines Hellkorn by means of Giemsa C-banding patterns and crosses with wheat–rye addition lines

Genome ◽  
1987 ◽  
Vol 29 (1) ◽  
pp. 58-62 ◽  
Author(s):  
Friedrich J. Zeller ◽  
Mari-Carmen Cermeño ◽  
Bernd Friebe
Keyword(s):  
Key Words ◽  
Secale Cereale ◽  
Banding Pattern ◽  
Addition Lines ◽  
Banding Patterns ◽  
Chromosome Addition ◽  
C Banding ◽  
Chromosome Addition Lines

Seven telotrisomic lines (1RS, 1RL, 2RS, 2RL, 3RS acro, 5RS, and 6RS), two double monotelosomic, and two double ditelosomic lines of Secale cereale cv. Heines Hellkorn were analyzed by means of Giemsa C-banding techniques. In crosses with several wheat–rye chromosome addition lines, the telosomic chromosomes in double ditelosomic lines 1/23 and 3/23 were found to be homologous to chromosomes 1R and 2RL of cv. Imperial rye. The C-banding pattern observed for the telosomes in these lines was similar to that detected in the 1R and 2R telosomics of the corresponding telotrisomic lines. Key words: Secale cereale, telotrisomics, double ditelosomics, C-banding pattern.

C-banding in 6x-Triticale x Secale cereale L. hybrid cytogenetics

1980 ◽  
Vol 57 (2) ◽  
pp. 75-79
Author(s):  
N. Jouve ◽  
N. Díez ◽  
M. Rodriguez
Keyword(s):  
Secale Cereale ◽  
C Banding ◽  
Secale Cereale L

N-banding analysis of rye chromosomes and the relationship between N-banded and C-banded heterochromatin

1984 ◽  
Vol 26 (6) ◽  
pp. 765-769 ◽  
Author(s):  
R. Schlegel ◽  
B. S. Gill
Keyword(s):  
Secale Cereale ◽  
Banding Pattern ◽  
Satellite Dna ◽  
Addition Lines ◽  
Chromosome Identification ◽  
Nonhistone Proteins ◽  
Banding Analysis ◽  
Secale Cereale L ◽  
C And N ◽  
The Relationship

Three of the seven chromosomes of rye (Secale cereale L.) 2R, 3R, and 6R have been identified individually by their N-banding pattern. Each of the N-banded chromosomes possesses a single band which is seen as one dot on each chromatid. Although the bands correspond with particular intercalary C-bands, they were found to be different from the remaining C-bands by sequential C- and N-banding analysis and thus reveal the heterogeneity of rye heterochromatin. It is described as C-banding and N-banding positive heterochromatin (C+N+). Similar to wheat and barley, the N-bands in rye appear to correspond to sites of (GAA)m (GAG)n sequence satellite DNA. In chromosome identification, the N-bands can be used as additional markers for the long arm of chromosome 2R (2RL), the short arm of chromosome 3R (3RS), and the long arm of chromosome 6R (6RL).Key words: heterochromatin, nonhistone proteins, banding, wheat–rye addition lines, rye.

Effet antioxydant et antihyperglycémiant du seigle (Secale cereale L.) et du sorgho (Sorghum bicolor L.) chez le rat Wistar rendu diabétique

2018 ◽  
Vol 16 (S1) ◽  
pp. S273-S283
Author(s):  
Z. Mami-Soualem ◽  
N. Brixi ◽  
C. Beghdad ◽  
M. Belarbi
Keyword(s):  
Sorghum Bicolor ◽  
Secale Cereale ◽  
Stress Oxydant ◽  
Habitudes Alimentaires ◽  
Diabète Sucré ◽  
Secale Cereale L ◽  
Vitamine C ◽  
Fibres Alimentaires ◽  
Peroxydation Lipidique ◽  
Sorghum Bicolor L

Le changement des habitudes alimentaires au cours de ces dernières années a abouti à l’apparition des maladies dites de civilisation, parmi elles le diabète sucré. Des approches diétothérapeutiques privilégient la consommation des produits céréaliers sous la forme la plus complète possible. Ce travail a pour but de tester l’efficacité des régimes expérimentaux préparés à base des grains complets des deux céréales, le sorgho (Sorghum bicolor L.) et le seigle (Secale cereale L.), sur la correction de l’hyperglycémie et des marqueurs du stress oxydant associés au diabète chez le rat mâle Wistar. Les résultats montrent que les rats diabétiques, soumis au régime seigle pendant quatre semaines, présentent une diminution significative de la glycémie qui atteint les 53,95 % à la fin de l’expérimentation, ce qui fait que leur taux en glycémie se rapproche de celui des normoglycémiques. Le sorgho blanc présente, d’une part, une diminution significative de la peroxydation lipidique intracellulaire et, d’autre part, une augmentation de l’activité de la glutathion peroxydase, la glutathion réductase ainsi que la vitamine C. Ces approches méthodologiques peuvent, en améliorant la connaissance de l’importance des céréales (fibres alimentaires solubles et des polyphénols) dans l’évolution du diabète, aboutir à des recommandations et à une éducation nutritionnelle du diabétique.

scholarly journals Production of Methane, Hydrogen and Ethanol from Secale cereale L. Straw Pretreated with Sulfuric Acid

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 1013 ◽  
Author(s):  
Jarosław Domański ◽  
Olga Marchut-Mikołajczyk ◽  
Weronika Cieciura-Włoch ◽  
Piotr Patelski ◽  
Urszula Dziekońska-Kubczak ◽  
...  
Keyword(s):  
Sulfuric Acid ◽  
Secale Cereale ◽  
Treatment Time ◽  
Ethanol Yield ◽  
Biofuel Production ◽  
Enzyme Treatment ◽  
Electricity Production ◽  
The European Union ◽  
Rye Straw ◽  
Secale Cereale L

The study describes sulfuric acid pretreatment of straw from Secale cereale L. (rye straw) to evaluate the effect of acid concentration and treatment time on the efficiency of biofuel production. The highest ethanol yield occurred after the enzyme treatment at a dose of 15 filter paper unit (FPU) per gram of rye straw (subjected to chemical hydrolysis with 2% sulfuric acid (SA) at 121 °C for 1 h) during 120 h. Anaerobic digestion of rye straw treated with 10% SA at 121 °C during 1 h allowed to obtain 347.42 L methane/kg volatile solids (VS). Most hydrogen was released during dark fermentation of rye straw after pretreatment of 2% SA, 121 °C, 1 h and 1% SA, 121 °C, 2 h—131.99 and 134.71 L hydrogen/kg VS, respectively. If the rye straw produced in the European Union were processed into methane, hydrogen, ethanol, the annual electricity production in 2018 could reach 9.87 TWh (terawatt-hours), 1.16 TWh, and 0.60 TWh, respectively.

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