BANDING ANALYSIS OF THE CHROMOSOMES OF MARMOSA MITIS (MURINE OPOSSUM)

1974 ◽  
Vol 16 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Frances M. Curcuru-Giordano ◽  
Richard G. Weed ◽  
Edmund C. Jenkins
Keyword(s):  
Bone Marrow ◽  
Chromosome Number ◽  
Y Chromosome ◽  
Cell Cultures ◽  
X Chromosome ◽  
Chromosome Elimination ◽  
Modal Chromosome Number ◽  
Banding Analysis ◽  
Lung Cell Cultures

The modal chromosome number of 14 has been confirmed in Marmosa mitis by examining 622 metaphase spreads derived from heart and lung cell cultures as well as from direct bone marrow preparations. Nine adult individuals, seven females and two males, were investigated. Preferential Y chromosome elimination was observed in both males. It was found that four of the seven chromosome pairs could be identified morphologically while Q-banding allowed differentiation of all chromosomes. The ASG technique revealed that one X chromosome was consistently heterochromatic.

B-chromosome Systems in the Greater Glider (Petauroides volans volans) (Marsupialia : Petauridae). I. B-chromosome Distribution

1985 ◽  
Vol 38 (1) ◽  
pp. 189 ◽  
Author(s):  
Leon R McQuade
Keyword(s):  
Bone Marrow ◽  
Chromosome Number ◽  
Y Chromosome ◽  
Stable Equilibrium ◽  
Bone Marrow Cells ◽  
B Chromosome ◽  
B Chromosomes ◽  
Diploid Chromosome Number ◽  
Body Tissues ◽  
Distinct Distribution

Variations in diploid chromosome number, due to the presence of B chromosomes, are found within the distribution of P. v. volans. B chromosomes vary in number between one and eight per animal, are mitotically stable in various body tissues and, unlike the Y chromosome in male P. v. volans, are not eliminated from bone marrow cells. Animals possessing B chromosomes have a distinct distribution, and it appears that a stable equilibrium between the forces of B chromosome accumulation or elimination is operating in those populations possessing these chromosomes.

scholarly journals Profiling of Chromosomal Complement in Nandidurga Goats of Karnataka

2021 ◽  
Author(s):  
Basavraj Inamdar ◽  
R. Nagaraja ◽  
H.M. Yathish ◽  
S. Naveen Kumar ◽  
G.S. Naveen Kumar ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Y Chromosome ◽  
X Chromosome ◽  
Relative Length ◽  
Lymphocyte Culture ◽  
Short Term ◽  
Blood Samples ◽  
Chromosomal Complement ◽  
Morphological Index ◽  
The Mean

Background: To characterize the chromosomal complement of Nandidurga goats. Methods: Blood samples for short term lymphocyte culture were collected from 5 bucks and 5 does from its breeding tract and then mitotic chromosomal spreads were accomplished. Result: The diploid chromosome number was found to be 60, consisting of 58 acrocentric autosomes and 2 sex chromosomes (X and Y). The X chromosome was found to be the longest acrocentric and Y chromosome was found to be sub metacentric. The mean mitotic drive was 64.5±2.01 and 63.15±1.30 percent in bucks and does, respectively. The mean relative length of autosomes varied from 2.08±0.24 to 4.81±0.12 in does and 2.09±0.09 to 4.70±0.11in bucks. The relative length of X chromosome in does was 5.14±0.17 and that in bucks was 4.92±0.21, whereas Y chromosome had a relative length of 1.99±0.20. The mean Arms Ratio, Centromeric Index and Morphological Index were 2.47, 31.62 and 396.14, respectively. This cytogenetic analysis indicates the normal chromosomal complement in the studied Nandidurga goats.

The Karyotype of Blainville’s Beaked Whale, Mesoplodon densirostris

2020 ◽  
pp. 1-6
Author(s):  
Ross Brookwell ◽  
Kimberly Finlayson ◽  
Jason P. van de Merwe
Keyword(s):  
Chromosome Number ◽  
Y Chromosome ◽  
X Chromosome ◽  
Organizer Region ◽  
Nucleolar Organizer Region ◽  
Nucleolar Organizer ◽  
Large Block ◽  
Beaked Whale

The karyotype of the Odontocete whale, <i>Mesoplodon densirostris</i>, has not been previously reported. The chromosome number is determined to be 2n = 42, and the karyotype is presented using G-, C-, and nucleolar organizer region (NOR) banding. The findings include NOR regions on 2 chromosomes, regions of heterochromatic variation, a large block of heterochromatin on the X chromosome, and a relatively large Y chromosome. The karyotype is compared to published karyograms of 2 other species of <i>Mesoplodon</i>.

scholarly journals First Report on Karyotypic, Morphometric and Meiotic Analysis of a Predatory Bombardier Beetle Pherosophus catoirai (Coleoptera: Carabidae) from Jammu region of Outer Himalayas, India

2021 ◽  
Vol 18 (4) ◽  
pp. 817-822
Author(s):  
Arshad Ayoub Bhatti ◽  
Nidhi Slathia ◽  
Manvi K
Keyword(s):  
Chromosome Number ◽  
Y Chromosome ◽  
X Chromosome ◽  
Chromosome Complement ◽  
Diploid Chromosome Number ◽  
Meiotic Analysis ◽  
Chromosome Y ◽  
First Report ◽  
Metaphase Stage ◽  
Spermatogonial Metaphase

Chromosomal studies and manual karyotyping are the aged techniques for determining the identity of a species on evolutionary scale; however, these techniques are simple, reliable and inexpensive to authenticate the existence of a particular species. In the present work, the chromosome complement and meiotic processes of a predatory bombardier beetle Pherosophus catoirai were investigated. This species presented 2n=35 as diploid chromosome number and the chromosomal formula was found to be 12m+8sm+12st+X0. Sex mechanism was X0 type with metacentric X chromosome. Y chromosome was absent in this species. Karyotype revealed small chromosomes except X chromosome which is found to be largest in the spermatogonial metaphase stage. Meiotic stages were pachytene, diplotene, diakinesis and metaphase-I. Present study may find importance to analyse evolution of chromosomes in order Coleoptera particularly in family Carabidae.

INTRASPECIES AUTOSOMAL POLYMORPHISM AND CHROMOSOME REPLICATION IN AKODON MOLINAE (RODENTIA: CRICETIDAE)

1969 ◽  
Vol 11 (2) ◽  
pp. 233-242 ◽  
Author(s):  
N. O. Bianchi ◽  
J. Contreras ◽  
F. N. Dulout
Keyword(s):  
Bone Marrow ◽  
Y Chromosome ◽  
X Chromosome ◽  
Sex Chromosome ◽  
Chromosome Analysis ◽  
Chromosome Replication ◽  
Chromosome 1 ◽  
Submetacentric Chromosome ◽  
Sex Chromatin ◽  
S Period

Cell spreads from bone marrow, spleen, testis and liver of four male and four female Akodon molinae (Rodentia:Cricetidae) were used for chromosome analysis and sex chromatin scoring. Chromosome replication at the beginning and end of the S period were analysed in bone marrow cells.In five animals (three males and two females) the diploid chromosome number was 42; the other three (1 male and 2 females) had a modal number of 43. In the former animals pairs 1,2,19,20 and the Y chromosome were easily identified morphologically. Chromosomes 1 were large and metacentric. In specimens with 43 chromosomes, pairs 2-20-XY were similar to those of animals with 42. Instead of having two number 1 homologues, these animals showed three unpaired chromosomes, one chromosome 1, one subterminal chromosome (1a) homologue of the long arm of the chromosome 1 and one submetacentric chromosome (1b) homologue of the short arm of the chromosome 1 Chromosomes 1a and 1b were considered to have arisen by a Robertsonian mechanism of centric fission of chromosome 1 plus a pericentric inversion.Studies of sex chromosome replication showed that the Y chromosome was the last to start and to end DNA synthesis in male complements. In females one X chromosome was the last to start replication. No late replicating X chromosome at the end of the S period was found. Coincidently, no sex chromatin could be detected in females.Analysis of late replication patterns in chromosomes 1, 1a and 1b, indicates that pericentric inversions can shift the replicating moment of the chromosomal regions involved in the rearrangement.

Evolution of a Y Chromosome from an X Chromosome

2019 ◽  
Author(s):  
Roberta Bergero ◽  
Jim Gardner ◽  
Deborah Charlesworth
Keyword(s):  
Y Chromosome ◽  
X Chromosome

Distribution of sister chromatid exchanges on the mouse chromosomes in vivo with reference to the replication properties of the X chromosome

1984 ◽  
Vol 26 (2) ◽  
pp. 152-157
Author(s):  
S. M. Singh ◽  
D. L. Reimer
Keyword(s):  
Bone Marrow ◽  
X Chromosome ◽  
Sister Chromatid ◽  
Bone Marrow Cells ◽  
Relative Length ◽  
Chromosome Length ◽  
Sister Chromatid Exchanges ◽  
Chromatid Exchanges ◽  
Marrow Cells

Frequency of sister chromatid exchanges (SCE) were recorded separately for different chromosomes from bone marrow cells of female mice of the two genetic strains (C3H/S and C57BL/6J). SCEs were evaluated following different doses of 5-bromo-2′deoxyuridine (BrdU) as nine hourly i.p. injections. The SCE per cell increased with increasing BrdU doses which was slightly higher in C3H/S than in the C57BL/6J. SCEs per cell were variable at every treatment – strain combination, possibly reflecting the heterogeneous nature of the bone marrow cells. In general, there is a positive correlation between SCE per chromosome and the relative chromosome length. Total SCEs on one of the large chromosomes (most likely the X chromosome), however, are significantly higher than expected on the basis of relative length alone. Most of this increase is attributable to one of the homologues of this chromosome, which is not in synchrony with the rest of the chromosomes and may represent the late-replicating X. These results when viewed in the light of replication properties of the heterochromatinized X, suggest a direct involvement of DNA replication in SCE formation and may argue against the replication point as the sole site for the SCEs.Key words: sister chromatid exchange, BrdU, recombination, replication, X chromosome.

Functional neutrophils from long-term murine bone marrow cell cultures

1987 ◽  
Vol 100 (1-2) ◽  
pp. 223-233 ◽  
Author(s):  
P.H. Hart ◽  
L.K. Spencer ◽  
P.A. Kenny ◽  
A.F. Lopez ◽  
P.J. McDonald ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Cell Cultures ◽  
Marrow Cell ◽  
Murine Bone Marrow ◽  
Murine Bone Marrow Cell
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