AVENA DAMASCENA: A NEW DIPLOID OAT SPECIES

1972 ◽  
Vol 14 (3) ◽  
pp. 645-654 ◽  
Author(s):  
Tibor Rajhathy ◽  
B. R. Baum
Keyword(s):  
Hybrid Sterility ◽  
Diploid Species ◽  
Pollen Sterility ◽  
The Other ◽  
Meiotic Pairing ◽  
Unique Structure ◽  
A Genome ◽  
Genome Homology ◽  
Genome Group ◽  
High Degree

A new diploid species of oat from Syria, named Avena damascena Rajhathy et Baum, is described. It has some macro- and micromorphological similarities to A. wiestii and A. prostrata, both diploids, and to tetraploid A. barbata. It differs from one or the other of them in its lemma tips, lodicules, epiblast and in the unique structure of the cuticle on the glumes. Avena damascena has a distinct symmetrical karyotype, designated Ad. The karyotype of A. prostrata is designated Ap. Avena damascena is isolated from A. prostrata by hybrid sterility and from the other diploids by cross-incompatibility. Avena damascena and A. prostrata are considered relicts of a common population because they retain a high degree of genome homology. Their chromosomes differ by two interdependent interchange complexes which led to a pollen sterility. Genome relationships in the A genome group of diploids are briefly discussed in terms of meiotic pairing patterns and karyotypes.

Putative diploid ancestors of 80-chromosome Glycine tabacina

Genome ◽  
1992 ◽  
Vol 35 (1) ◽  
pp. 140-146 ◽  
Author(s):  
R. J. Singh ◽  
K. P. Kollipara ◽  
F. Ahmad ◽  
T. Hymowitz
Keyword(s):  
Adventitious Roots ◽  
Chromosome Doubling ◽  
Colchicine Treatment ◽  
Meiotic Pairing ◽  
B Genome ◽  
Specific Hybridization ◽  
A Genome ◽  
Genome Homology ◽  
Glycine Tabacina ◽  
Natural Mutation

The objective of this study was to discover the diploid progenitors of 80-chromosome Glycine tabacina with adventitious roots (WAR) and no adventitious roots (NAR). Three synthetic amphiploids were obtained by somatic chromosome doubling. These were (i) (G. latifolia, 2n = 40, genome B1B1,) × (G. microphylla, 2n = 40, genome BB) = F1(2n = 40, genome BB1) – 0.1% colchicine treatment (CT) – 2n = 80, genome BBB1B1; (ii) (G. canescens, 2n = 40, genome AA) × G. microphylla, 2n = 40, genome BB) = F1 (2n = 40, genome AB) – (CT) – 2n = 80, genome AABB; (iii) (G. latifolia, 2n = 40, B1B1) × G. canescens, 2n = 40, AA) = F1 (2n = 40, genome AB1) – (CT) – 2n = 80, genome AAB1B1. The segmental allotetraploid BBB1B1 was morphologically similar to the 80-chromosome G. tabacina (WAR), but meiotic pairing data in F1 hybrids did not support the complete genomic affinity. Despite normal diploid-like meiosis in allotetraploids AABB and AAB1B1, AABB was completely fertile, while pod set in AAB1B1 was very sparse. Morphologically, allotetraploid AABB was indistinguishable from the 80-chromosome G. tabacina (NAR) but in their F1 hybrids, the range of univalents at metaphase I was wide (4–44). The allotetraploid AAB1B1 did not morphologically resemble the 80-chromosome G. tabacina (NAR). However, the F1 hybrid of AABB × AAB1B1 showed normal meiosis with an average chromosome association (range) of 1.7 I (0–4) + 39.2 II (38–40). Based on this information, we cannot correctly deduce the diploid progenitor species of the 80-chromosome G. tabacina (NAR). The lack of exact genome homology may be attributed to the geographical isolation, natural mutation, and growing environmental conditions since the inception of 80-chromosome G. tabacina. Thus, it is logical to suggest that the 80-chromosome G. tabacina (NAR) is a complex, probably synthesized from A genome (G. canescens, G. clandestina, G. argyrea, G. tomentella D4 isozyme group) and B genome (G. latifolia, G. microphylla, G. tabacina) species, and the 80-chromosome G. tabacina (WAR) complex was evolved through segmental allopolyploidy from the B genome species.Key words: Glycine spp., allopolyploidy, colchicine, genome, intra- and inter-specific hybridization, polyploid complex.

Differences in meiotic pairing induction between the A genomes of Triticum boeoticum Boiss. and T. urartu Tum. using diploid species of the subtribe Triticineae as analysers

Genome ◽  
1987 ◽  
Vol 29 (6) ◽  
pp. 891-893 ◽  
Author(s):  
H. Lucas ◽  
J. Jahier
Keyword(s):  
Interspecific Hybrids ◽  
Diploid Species ◽  
Distinct Species ◽  
The Other ◽  
Meiotic Behaviour ◽  
Meiotic Pairing ◽  
Haynaldia Villosa ◽  
Pollen Mother Cells ◽  
Triticum Boeoticum ◽  
Mother Cells

The number of associations between chromosome arms in the pollen mother cells of the hybrid Triticum boeoticum × T. urartu is similar to that in the pollen mother cells of the parental accessions. The latter two species were crossed with the following diploid species: T. tauschii, T. comosum, T. umbellulatum, and Haynaldia villosa. The meiotic behaviour of the hybrids showed that the chromosomes of T. urartu share more homology with the diploid Triticum species than do those of T. boeoticum. On the other hand, there is more pairing in the hybrid T. boeoticum × H. villosa than in T. urartu × H. villosa. These results confirm that T. boeoticum and T. urartu are distinct species. Key words: Triticineae, interspecific hybrids, meiotic behaviour, speciation.

Restriction fragment length polymorphism based phylogenetic analysis of Avena L.

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1279-1284 ◽  
Author(s):  
Rita Alicchio ◽  
Lina Aranci ◽  
Lucia Conte
Keyword(s):  
Fragment Length Polymorphism ◽  
Phylogenetic Analyses ◽  
Diploid Species ◽  
The Other ◽  
Molecular Approach ◽  
Polyploid Species ◽  
Species Relationships ◽  
Biochemical Traits ◽  
A Genome ◽  
Restriction Fragment Length

We report a molecular approach to the study of the phylogenetic relationships of Avena diploid and polyploid species based on RFLP detected with three cDNA probes of nuclear genes belonging to multigenic families (low pI α-amylase, avenin, and globulin). All the probes were highly informative in the detection of polymorphism between oat species. Associations between species were determined from cluster (UPGMA) analysis based on distance values calculated from RFLP data separately for each of the two levels of ploidy. Results were in general agreement with morphology based phylogenetic analyses, confirming the large differentiation among A and C genomes in the evolution of diploid species and the genetic homogeneity among A. brevis, A. strigosa, and A. nuda and the recently discovered A. atlantica. A certain divergence was observed between two endemic species (A. canariensis and A. damascena) and the other diploid species with the A genome. The analysis of tetraploid species relationships confirms the differentiation of the barbata complex (A. wiestii, A. barbata, A. abyssinica, and A. vaviloviana) from the maroccana–murphyi–agadiriana group, which, despite some similarities in morphological and biochemical traits, seems to have accumulated deep genetic differences along its evolutionary pathway.Key words: Avena genomes, genetic distance, ploidy, RFLP, multigenic families.

The genomes of Glycine canescens F.J. Herm., and G. tomentella Hayata of Western Australia and their phylogenetic relationships in the genus Glycine Willd.

Genome ◽  
1998 ◽  
Vol 41 (5) ◽  
pp. 669-679 ◽  
Author(s):  
Ram J Singh ◽  
Krishna P Kollipara ◽  
Theodore Hymowitz
Keyword(s):  
Western Australia ◽  
Phylogenetic Relationships ◽  
Diploid Species ◽  
South Australia ◽  
Its Region ◽  
Genomic Diversity ◽  
Complex Species ◽  
Genome Species ◽  
A Genome ◽  
Genome Group

A multidisciplinary approach is an extremely powerful tool for determining genomic diversity and establishing genomic relationships within and among species. This study used cytogenetics and a molecular method (ITS of the rDNA) to uncover genomic diversity in Glycine canescens and Glycine tomentella and to establish their phylogenetic relationships with the other diploid species of the genus Glycine. Cytogenetics revealed that G. canescens accessions (PIs 583944, 583946, 583953, and 591575) from Western Australia were genomically similar. However, they were differentiated by a paracentric inversion from the standard G. canescens (PI 440932) collected from South Australia. By contrast, G. tomentella (2n = 40) accessions from Western Australia were highly diverse genomically. Cytogenetics and ITS investigations separated the diploid G. tomentella accessions in Australia into four distinct groups. The genome symbols DD (isozyme group D3; PI 505222), D1D1 (isozyme group D5; PI 505301), D2D2 (isozyme group D5; PI 505203), and D3D3 (isozyme group D4; PI 441000) are being assigned to these four groups. The D1 and D2 genome group accessions are distributed in Western Australia. The D3-genome group of G. tomentella accessions are morphologically similar neither to A-genome species nor to the D-, D1-, or D2-genome groups. However, the D3-genome group was phylogenetically grouped with the A-genome species, while the D-, D1-, and D2-genome groups showed a close relationship with E-, H-, and I-genome species. This study demonstrates that diploid G. tomentella of Western Australia is a complex species, and from an evolutionary viewpoint, it is actively radiating out into several genomic variants.Key words: Glycine spp., soybean, genome, cytogenetics, ITS region.

scholarly journals Phylogenetic Relationships of Tetraploid AB-Genome Avena Species Evaluated by Means of Cytogenetic (C-Banding and FISH) and RAPD Analyses

2010 ◽  
Vol 2010 ◽  
pp. 1-13 ◽  
Author(s):  
E. D. Badaeva ◽  
O. Yu. Shelukhina ◽  
S. V. Goryunova ◽  
I. G. Loskutov ◽  
V. A. Pukhalskiy
Keyword(s):  
Diploid Species ◽  
5S Rdna ◽  
Banding Technique ◽  
Diploid Progenitor ◽  
Genome Species ◽  
C Banding ◽  
A Genome ◽  
Different Types ◽  
Genome Group

Tetraploid oat species Avena abyssinica, A. vaviloviana, A. barbata, and A. agadiriana were studied using C-banding technique, in situ hybridization with the 45S and 5S rDNA probes, and RAPD analysis in comparison with the diploid species carrying different types of the A-genome (A. wiestii, As; A. longiglumis, Al; A. canariensis, Ac; A. damascena, Ad, A. prostrata, Ap). The investigation confirmed that all four tetraploids belong to the same AB-genome group; however A. agadiriana occupies distinct position among others. The C-banding, FISH, and RAPD analyses showed that Avena abyssinica, A. vaviloviana, and A. barbata are very similar; most probably they originated from a common tetraploid ancestor as a result of minor translocations and alterations of C-banding polymorphism system. AB-genome species are closely related with the A-genome diploids, and an As-genome species may be regarded as the most probable donor of their A-genome. Although their second diploid progenitor has not been identified, it seems unlikely that it belongs to the As-genome group. The exact diploid progenitors of A. agadiriana have not been determined; however our results suggest that at least one of them could be related to A. damascena.

EVOLUTION IN THE GENUS AVENA: INHERITANCE OF DIFFERENT FORMS OF RIBULOSE DIPHOSPHATE CARBOXYLASE

1975 ◽  
Vol 17 (3) ◽  
pp. 337-344 ◽  
Author(s):  
M. W. Steer
Keyword(s):  
Chloroplast Genome ◽  
Species Distribution ◽  
Diploid Species ◽  
Disc Electrophoresis ◽  
The Other ◽  
Avena Species ◽  
A Genome ◽  
C Genome ◽  
Evolutionary Paths ◽  
Ribulose Diphosphate Carboxylase

Disc electrophoresis of ribulose diphosphate carboxylases from Avena species in polyacrylamide gels of varying concentrations reveals the presence of two distinct forms of the enzyme. One migrates faster than the other and is found exclusively in species possessing the A genome. The other is confined to the C genome species (A. pilosa, A. ventricosa, A. clauda). The association of another characteristic of this enzyme (presence of stromacentres in the chloroplasts) with the A genome was reported previously. Observations on the recently described species A. prostrata, A. canariensis, A. damascena, and A. murphyi show that they all possess stromacentres, confirming reports that they all contain the A genome. Examination of amphiploid hybrids (from crosses between various diploid species and A. sativa) has shown that the mobility character is inherited maternally, and is located on the chloroplast genome. All these hybrids have the A genome and all have stromacentres. The results are discussed in the light of recent findings on the structure and synthesis of this enzyme. Consideration of the species distribution of the different forms of carboxylase places certain restrictions on the possible evolutionary paths from diploids to tetraploids and hexaploids.

Relationship Between Level of Income and Method of Contribution and Appointment of Islamic Waqf Bank as an Agent in Collecting Waqf Fund

2014 ◽  
Vol 2 (2) ◽  
pp. 15
Author(s):  
Muhammad Ridhwan Ab. Aziz ◽  
Mohd Asyraf Yusof ◽  
Fuadah Johari ◽  
Hisham Sabri
Keyword(s):  
Support Services ◽  
Quantitative Research ◽  
Financial Institution ◽  
The Other ◽  
Strong Tendency ◽  
Trust Fund ◽  
Cost Of Living ◽  
Good Education ◽  
Unique Structure ◽  
The Relationship

Receiving a good education helps empower people knowledge, thus making them strong enough to look after themselves in any given situation. It keeps oneself aware of given surrounding as well as the rules and regulations of the society they living in. Moreover, the technology that we use today is a result from the advancement and improvement of education. On the other hand Islamic waqf bank is a special designed financial institution in Islam. This bank will benefit the student and also their parents, due to its unique structure that could finance students‟ education in term of fees and cost of living. Islamic waqf bank uses the concept of cash waqf in terms of funding the education. While cash waqf is a trust fund established with money to support services for mankind‟s benefits in the name of Allah. The objective of this article is to examine the relationship between level of income and contribution method of cash waqf fund in Islamic waqf bank as well as the appointment of an agent in collecting waqf fund in Islamic waqf bank. The methodology of this research is a quantitative research towards 287 respondents among Muslim public in this country. The general finding of this article shows that, with proper contribution method and the appointment of Islamic waqf bank as an agent in collecting the cash waqf fund, there is a strong tendency that the Islamic waqf bank‟s operation will be run effectively.

C-Terminal amino acid sequence of chicken pepsinogen and its homology with sequences of other acid proteases

1980 ◽  
Vol 45 (4) ◽  
pp. 1144-1154 ◽  
Author(s):  
Miroslav Baudyš ◽  
Helena Keilová ◽  
Vladimír Kostka
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
The Other ◽  
Terminal Sequence ◽  
Terminal Part ◽  
Terminal Amino Acid ◽  
Tryptic Peptides ◽  
Terminal Amino ◽  
High Degree ◽  
Terminal Amino Acid Sequence

To determine the primary structure of the C-terminal part of the molecule of chicken pepsinogen the tryptic, chymotryptic and thermolytic digest of the protein were investigated and peptides derived from this region were sought. These peptides permitted the following 21-residue C-terminal sequence to be determined: ...Ile-Arg-Glu-Tyr-Tyr-Val-Ile-Phe-Asp-Arg-Ala-Asn-Asn-Lys-Val-Gly-Leu-Ser-Pro-Leu-Ser.COOH. A comparison of this structure with the C-terminal sequential regions of the other acid proteases shows a high degree of homology between chicken pepsinogen and these proteases (e.g., the degree of homology with respect to hog pepsinogen and calf prochymosin is about 66%). Additional tryptic peptides, derived from the N-terminal part of the zymogen molecule whose amino acid sequence has been reported before, were also obtained in this study. This sequence was extended by two residues using an overlapping peptide. An ancillary result of this study was the isolation of tryptic peptides derived from other regions of the zymogen molecule.

Chromosomal organization of a sequence related to LTR-like elements of Ty1-copia retrotransposons in Avena species

Genome ◽  
1999 ◽  
Vol 42 (4) ◽  
pp. 706-713 ◽  
Author(s):  
Concha Linares ◽  
Antonio Serna ◽  
Araceli Fominaya
Keyword(s):  
In Situ Hybridization ◽  
Diploid Species ◽  
D Genome ◽  
Specific Sequence ◽  
Chromosomal Organization ◽  
Intergenomic Translocations ◽  
A Genome ◽  
Slot Blot Analysis ◽  
Copia Retrotransposons

A repetitive sequence, pAs17, was isolated from Avena strigosa (As genome) and characterized. The insert was 646 bp in length and showed 54% AT content. Databank searches revealed its high homology to the long terminal repeat (LTR) sequences of the specific family of Ty1-copia retrotransposons represented by WIS2-1A and Bare. It was also found to be 70% identical to the LTR domain of the WIS2-1A retroelement of wheat and 67% identical to the Bare-1 retroelement of barley. Southern hybridizations of pAs17 to diploid (A or C genomes), tetraploid (AC genomes), and hexaploid (ACD genomes) oat species revealed that it was absent in the C diploid species. Slot-blot analysis suggested that both diploid and tetraploid oat species contained 1.3 × 104 copies, indicating that they are a component of the A-genome chromosomes. The hexaploid species contained 2.4 × 104 copies, indicating that they are a component of both A- and D-genome chromosomes. This was confirmed by fluorescent in situ hybridization analyses using pAs17, two ribosomal sequences, and a C-genome specific sequence as probes. Further, the chromosomes involved in three C-A and three C-D intergenomic translocations in Avena murphyi (AC genomes) and Avena sativa cv. Extra Klock (ACD genomes), respectively, were identified. Based on its physical distribution and Southern hybridization patterns, a parental retrotransposon represented by pAs17 appears to have been active at least once during the evolution of the A genome in species of the Avena genus.Key words: chromosomal organization, in situ hybridization, intergenomic translocations, LTR sequence, oats.

scholarly journals Physical mapping of repetitive oligonucleotides facilitates the establishment of a genome map-based karyotype to identify chromosomal variations in peanut

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Liuyang Fu ◽  
Qian Wang ◽  
Lina Li ◽  
Tao Lang ◽  
Junjia Guo ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Physical Mapping ◽  
Tandem Repeats ◽  
Genetic Research ◽  
Diploid Species ◽  
Reference Sequence ◽  
A Genome ◽  
Genome Map ◽  
Chromosomal Variants

Abstract Background Chromosomal variants play important roles in crop breeding and genetic research. The development of single-stranded oligonucleotide (oligo) probes simplifies the process of fluorescence in situ hybridization (FISH) and facilitates chromosomal identification in many species. Genome sequencing provides rich resources for the development of oligo probes. However, little progress has been made in peanut due to the lack of efficient chromosomal markers. Until now, the identification of chromosomal variants in peanut has remained a challenge. Results A total of 114 new oligo probes were developed based on the genome-wide tandem repeats (TRs) identified from the reference sequences of the peanut variety Tifrunner (AABB, 2n = 4x = 40) and the diploid species Arachis ipaensis (BB, 2n = 2x = 20). These oligo probes were classified into 28 types based on their positions and overlapping signals in chromosomes. For each type, a representative oligo was selected and modified with green fluorescein 6-carboxyfluorescein (FAM) or red fluorescein 6-carboxytetramethylrhodamine (TAMRA). Two cocktails, Multiplex #3 and Multiplex #4, were developed by pooling the fluorophore conjugated probes. Multiplex #3 included FAM-modified oligo TIF-439, oligo TIF-185-1, oligo TIF-134-3 and oligo TIF-165. Multiplex #4 included TAMRA-modified oligo Ipa-1162, oligo Ipa-1137, oligo DP-1 and oligo DP-5. Each cocktail enabled the establishment of a genome map-based karyotype after sequential FISH/genomic in situ hybridization (GISH) and in silico mapping. Furthermore, we identified 14 chromosomal variants of the peanut induced by radiation exposure. A total of 28 representative probes were further chromosomally mapped onto the new karyotype. Among the probes, eight were mapped in the secondary constrictions, intercalary and terminal regions; four were B genome-specific; one was chromosome-specific; and the remaining 15 were extensively mapped in the pericentric regions of the chromosomes. Conclusions The development of new oligo probes provides an effective set of tools which can be used to distinguish the various chromosomes of the peanut. Physical mapping by FISH reveals the genomic organization of repetitive oligos in peanut chromosomes. A genome map-based karyotype was established and used for the identification of chromosome variations in peanut following comparisons with their reference sequence positions.

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