KARYOTYPE ANALYSIS OF HEXAPLOID TRITICALE

1971 ◽  
Vol 13 (3) ◽  
pp. 585-591 ◽  
Author(s):  
S. Shigenaga ◽  
E. N. Larter
Keyword(s):  
Karyotype Analysis ◽  
Chromosome Complement ◽  
Hexaploid Triticale ◽  
The Cross ◽  
Haploid Complement

Karyotype analysis of hexaploid triticale cultivar 'Rosner' (2n = 42 = AABBRR) revealed that its chromosome complement consists of five satellited, five median, nine submedian, and two subterminal chromosomes. In addition to Rosner, the karyotype of the F1 hybrid (2n = 28 = ABRR) obtained from the cross between Rosner and rye cultivar 'Prolific' was analysed. Since in the ABRR hybrid only the chromosomes of the R genome were present in pairs, it was possible to classify the chromosomes of rye separately from the single chromosomes of the A and B genomes of wheat. From the idiogram constructed from these studies it was demonstrated that the rye chromosomes of the Rosner karyotype were the longest of the entire haploid complement; three of these were satellited, two were median, and two were submedian in structure.

scholarly journals Orcein and DAPI-stained karyotype analysis of Alocasia macrorrhizos (L.) G. Don

2021 ◽  
Vol 45 (1) ◽  
pp. 27-35
Author(s):  
Ashma Ahmed Warasy
Keyword(s):  
Karyotype Analysis ◽  
Chromosome Complement ◽  
Asymmetry Index ◽  
Lower Amount ◽  
Interphase Nuclei ◽  
Karyotype Asymmetry ◽  
Karyotype Analyses ◽  
Dapi Banding ◽  
Gradient Type ◽  
Chromosomal Length

Karyotype analyses are required for the identification, characterization, and genetic improvement of any organism. Alocasia macrorrhizos (L.) G. Don. was investigated cytogenetically to determine the karyotypic features. Complex chromocenter type, of interphase nuclei, and gradient type of prophase chromosomes were found in this study. Alocasia macrorrhizos was found to possesses 2n=28 chromosomes. The total length of the 2n chromosome complement was recorded as 98.83±1.39 μm. The range of chromosomal length was 2.50±0.10-4.70±0.10 μm. A gradual decrease in chromosomal length was observed. The total form (TF%) value was found to be 43.58%, Karyotype symmetry index (Syi %) was 77.00 % and karyotype asymmetry index (AsK %) was 56.66%. The centromeric formula was 18m+4sm+2ac, representing asymmetric karyotype. In DAPI banding, the 1.48% positive banded region indicates the lower amount of AT rich repeats in this material. Therefore, Alocasia macrorrhizos could be authentically characterized through karyotype analysis. J. Bangladesh Acad. Sci. 45(1); 27-35: June 2021

scholarly journals Karyotype analysis with orcein and CMA in two species of Alocasia (Schott) G. Don (Araceae)

1970 ◽  
Vol 40 (1) ◽  
pp. 53-56 ◽  
Author(s):  
Syeda Sharmeen Sultana ◽  
Hosne Ara ◽  
Sheikh Shamimul Alam
Keyword(s):  
Chromosome Number ◽  
Key Words ◽  
Karyotype Analysis ◽  
Chromosome Complement ◽  
Taxonomic Status ◽  
The Other ◽  
First Report ◽  
Total Length ◽  
Internet Information ◽  
Chromosomal Length

Alocasia fallax Schott and A. odora (Roxb.) Koch (Araceae) were investigated cytogenetically to confirm their taxonomic status. There is no report of 2n chromosome number for A. fallax in the available literature and internet information. Therefore the 2n chromosome number (2n = 28) found in this study is probably the first report for A. fallax. Alocasia odora showed exactly double 2n chromosome number (2n = 56) from A. fallax. In addition to chromosome number, the other karyotypic features of A. odora were exactly double for that of A. fallax. The centromeric formulae of A. fallax was 24 m + 4 sm whereas it is just double in A. odora. Total length of 2n chromosome complement of A. odora (62.58 μm) was almost double to A. fallax. The range of chromosomal length of the two species was almost same. Moreover, A. odora plant is much taller than A. fallax. All of these data suggests that A. odora might be an autotetraploid of A. fallax which in course of evolution had undergone some changes in GC-rich repeats. Key words: Alocasia; CMA; Karyotype analysis DOI: http://dx.doi.org/10.3329/bjb.v40i1.7998 Bangladesh J. Bot. 40(1): 53-56, 2011 (June)

Yield improvement of oilseed rape (Brassica napus L.) in a low rainfall environment. I. Utilization of genes for early flowering in primary and secondary gene pools

1992 ◽  
Vol 43 (3) ◽  
pp. 609 ◽  
Author(s):  
N Thurling ◽  
R Kaveeta
Keyword(s):  
Brassica Napus ◽  
Chromosome Number ◽  
Flowering Time ◽  
Chromosome Complement ◽  
Early Flowering ◽  
Gene Pools ◽  
Yield Improvement ◽  
Two Generations ◽  
The Cross ◽  
Recurrent Backcrossing

The extent to which time to flowering of a Brassica napus commercial cultivar could be reduced through utilization of genes in its primary and secondary pools was examined with particular reference to yield improvement in lower rainfall environments. The B. napus breeding line RU2 and the B. campestris population Chinoli C42, which were used as sources of early flowering genes to be incorporated into the commercial B. napus cultivar Wesbrook, flowered significantly earlier than Wesbrook with or without vernalization and/or long days. In the cross of Wesbrook with RU2, the substantial variation in flowering time in the F2 was highly heritable (hn2 = 0.79), and some plants in the F2 and first backcross to Wesbrook flowered earlier than the early flowering parent RU2. Many lines flowering much earlier than Wesbrook were still obtained after two generations of recurrent backcrossing to Wesbrook and subsequent selfing without selection at any stage. Variation in flowering time among these lines primarily reflected differences in growth rate as measured by the rate of leaf node development. F2 plants derived from the cross between Wesbrook and the B. campestris population Chinoli C42 varied widely in chromosome number and flowering time, but there was no relationship between pre-anthesis development and chromosome number. Two generations of recurrent backcrossing to Wesbrook and subsequent selfing without selection at any stage produced a series of lines all having the normal B. napus chromosome complement. All these lines flowered significantly earlier than Wesbrook, and the earliest flowering line flowered at the same time as the early flowering B. campestris parent. The implications of these results are discussed with particular reference to developing agronomically superior B. napus cultivars closely adapted to lower rainfall environments in the Western Australian wheatbelt and elsewhere in southern Australia.

Studi Cromosomici nella Sindrome di Turner

1965 ◽  
Vol 14 (2) ◽  
pp. 182-199 ◽  
Author(s):  
F. Franceschini ◽  
B. Dallapiccola ◽  
N. Ricci ◽  
B. Ventimiglia
Keyword(s):  
Chromosome Number ◽  
Karyotype Analysis ◽  
Chromosome Complement ◽  
Turner's Syndrome ◽  
Late Replication ◽  
Turner’S Syndrome ◽  
Cytogenetic Studies

SUMMARYCytogenetic studies were performed in six clinically typical cases of Turner's syndrome. In five of them an XO chromosome complement was observed. In the sixth case the chromosome number appeared normal, but karyotype analysis revealed the presence of three large metacentric chromosomes similar to No. 3. A diagnosis of presumptive isochromosome for the long arm of X was suggested by the autoradiographic evidence of late replication and by the presence of larger than normal Barr bodies and drumsticks.

scholarly journals Karyotype analysis in Chaerophyllum cicutaria Vill. with special emphasis on satellited chromosomes

2014 ◽  
Vol 56 (1) ◽  
pp. 61-72
Author(s):  
Włodzimierz Chojnacki ◽  
Jerzy Bohdanowicz
Keyword(s):  
Chromosome Number ◽  
Karyotype Analysis ◽  
Natural Populations ◽  
Chromosome Complement ◽  
Chromosome Morphology ◽  
Single Pair

Karyological studies were carried out on plants of <em>Chaerophyllum cicutaria (Umbelliferae)</em>, which came from both lowland and montane natural populations. The chromosome number in all the examined plants was 2n = 22 and their karyotypes, though similar in general, showed some minute but distinct differences. There was` a single pair of SAT chromosomes in the chromosome complement. They had compound satellites divided into two or three segments. Seven morphological types of SAT chromosomes differing in number and size of satellite segments were distinguished. With respect to SAT chromosome morphology, the species showed both intra- and interpopulational karyological variation.

CHROMOSOME LOCATION OF TWO LEAF RUST RESISTANCE GENES TRANSFERRED FROM TRITICUM SPELTOIDES TO T. AESTIVUM

1980 ◽  
Vol 22 (3) ◽  
pp. 381-389 ◽  
Author(s):  
J. Dvořák ◽  
D. R. Knott
Keyword(s):  
Leaf Rust ◽  
Resistance Genes ◽  
Chinese Spring ◽  
Rust Resistance ◽  
Meiotic Chromosome ◽  
Chromosome Complement ◽  
Leaf Rust Resistance ◽  
The Cross ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Two homoeologous-recombination-produced gene transfer lines in which leaf rust resistance genes from Triticum speltoides Tausch were incorporated into the chromosome complement of Triticum aestivum L. emend. Thell. were analyzed. One line, F-7-3, originated from the cross T. aestivum cv. Neepawa × T. speltoides accession F and the other line, E-11-14, originated from the cross T. aestivum cv. Manitou × T. speltoides accession E. The lines were crossed with Chinese Spring monosomics. Segregation of the F2 progenies from monosomic F1's indicated that both genes were on chromosome 1B. The lines were then crossed with Chinese Spring ditelosomics 1BS and 1BL and the F1, plants were testcrossed to Manitou. Meiotic chromosome pairing in the F1 plants and the segregation of the telosomes and resistance in the testcrosses provided evidence that both gene transfers involved chromosome arm 1BL. The exchange points showed 41% and 4% recombination with the centromere in F-7-3 and E-11-14, respectively. Recombination between the transferred alien chromatin and the chromatin of the recipient wheat chromosome is either severely reduced or entirely absent when the wheat diploidizing mechanism is active.

scholarly journals Karyotype analysis in lignosus bean (Dipogon lignosus) and lablab bean (Lablab purpureus)

1970 ◽  
Vol 9 (1) ◽  
pp. 27-36 ◽  
Author(s):  
MA Ali ◽  
MM Hasan ◽  
MS Mia ◽  
QN Ahmad
Keyword(s):  
Plant Breeding ◽  
Karyotype Analysis ◽  
Lablab Purpureus ◽  
Root Tips ◽  
Genetic Studies ◽  
Slide Preparation ◽  
Bean Genotype ◽  
The Individual ◽  
Germinated Seeds ◽  
Haploid Complement

The experiment was conducted in the cytogenetics laboratory of the Department of Genetics and Plant Breeding of Bangladesh Agricultural University during July 2008 to September 2009 aiming to analyze and compare the karyotypes of two genotypes of Lignosus bean (Dipogon lignosus L.) and Lablab bean (Lablab purpureus L.). Root tips from germinated seeds were collected, pretreated and fixed in acetic alcohol for using in slide preparation. Chromosome measurements were done from the prints of the photomicrographs of prometaphase plates. In both the genotypes, 2n = 22 chromosomes was found. The range of length of the individual chromosome was 1.22 to 2.17μ in Lignosus bean, and 1.17 to 3.00μ in Lablab bean. In the Lignosus bean genotype, the haploid complement consisted of 11 metacentric (m) chromosomes with 3 individually identifiable ones but incase of Lablab bean the haploid complement consisted of 11 m chromosomes with 5 individually identifiable ones. All identifiable chromosomes were metacentric. This karyotype analysis of Lignosus bean and Lablab bean will be useful for further genetic studies. Keywords: Chromosomal characteristics; Karyotype analysis; Bean; Derivation DOI: http://dx.doi.org/10.3329/jbau.v9i1.8740 JBAU 2011; 9(1): 27-36

New cytogenetic information on Allium iranicum (Alliaceae) from Iran

Biologia ◽  
2006 ◽  
Vol 61 (4) ◽  
Author(s):  
Seyed Ghaffari
Keyword(s):  
Detailed Analysis ◽  
Related Species ◽  
Karyotype Analysis ◽  
Chromosome Complement ◽  
Chromosome Behaviour ◽  
Homologous Chromosomes ◽  
Pollen Mother Cells ◽  
Satellite Chromosomes ◽  
Mother Cells ◽  
Metaphase I

AbstractKaryotype analysis and chromosome behaviour in tetraploid Allium iranicum is reported. The somatic karyotype 2n = 32, consists of 12 pairs of metacentric chromosomes, two pairs of submetacentric chromosomes and two pairs of submetacentric satellite chromosomes. Chromosome complement follows two sets of 16 pairs of homologous chromosomes. A detailed analysis of Pachytene, Diplotene and Metaphase I of meiosis in pollen mother cells in this taxon showed that the most common chromosome configurations were bivalents at all subphases mentioned. It is concluded that A. iranicum is most likely a natural allotetraploid and certainly differs from related species A. ampeloprasum, A. commutatum and A. porrum.

A New Approach to the Demonstration of Oxidase-Localization Using Tannic Acid Or Catechin

1973 ◽  
Vol 31 ◽  
pp. 698-699
Author(s):  
V. Mizuhira ◽  
Y. Futaesaku
Keyword(s):  
Cross Section ◽  
Tannic Acid ◽  
Elastic Fiber ◽  
The Other ◽  
New Approach ◽  
Tissue Block ◽  
Active Reaction ◽  
The Cross

Previously we reported that tannic acid is a very effective fixative for proteins including polypeptides. Especially, in the cross section of microtubules, thirteen submits in A-tubule and eleven in B-tubule could be observed very clearly. An elastic fiber could be demonstrated very clearly, as an electron opaque, homogeneous fiber. However, tannic acid did not penetrate into the deep portion of the tissue-block. So we tried Catechin. This shows almost the same chemical natures as that of proteins, as tannic acid. Moreover, we thought that catechin should have two active-reaction sites, one is phenol,and the other is catechole. Catechole site should react with osmium, to make Os- black. Phenol-site should react with peroxidase existing perhydroxide.

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