SATELLITE ASSOCIATION IN HUMAN LYMPHOCYTES AND SODIUM CITRATE CONCENTRATION OF HYPOTONIC (SPREADING) SOLUTION

1971 ◽  
Vol 13 (1) ◽  
pp. 115-118 ◽  
Author(s):  
Howard R. Nankin
Keyword(s):  
Sodium Citrate ◽  
Human Lymphocytes ◽  
Satellite Association ◽  
Citrate Concentration ◽  
Acrocentric Chromosomes ◽  
The Relationship

The present investigation examined the relationship between satellite association in lymphocytes and sodium citrate concentration of the hypotonic solutions used to aid in spreading chromosomes. With reduction of sodium citrate from 0.9%, to 0.75%, and to 0.6%, the percentage of acrocentric chromosomes engaged in satellite association went from 32.7%, to 26.8%, and to 19.1% respectively. The reductions appeared to be equally divided between the D group and G group chromosomes.

Optimization of Gold Nanoparticles Synthesis using Design of Experiments Technique

2017 ◽  
Vol 68 (7) ◽  
pp. 1518-1423
Author(s):  
Adina Turcu Stiolica ◽  
Mariana Popescu ◽  
Maria Viorica Bubulica ◽  
Carmen Nicoleta Oancea ◽  
Claudiu Nicolicescu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Zeta Potential ◽  
Design Of Experiments ◽  
Process Model ◽  
Sodium Citrate ◽  
Drug Carriers ◽  
Chloroauric Acid ◽  
Gold Colloids ◽  
Citrate Concentration ◽  
Vis Spectroscopy

Gold nanoparticles are considered the newest drug carriers for different diseases. Therefore it is appropriate continuous optimization of their preparation. In this study, gold colloids with an average size of 1 - 26 nm were obtained by the reduction of tetrachloroauric acid with trisodium citrate. The nanomaterials were characterized by UV-Vis spectroscopy and dynamic light scattering technique. In addition, zeta potential was measured for samples synthesized in order to determine the stability of the colloids. A Two-level Full Factorial design was chosen to determine the optimum set of process parameters (chloroauric acid concentration and sodium citrate concentration) and their effect on various gold nanoparticles characteristics (size and zeta potential). These effects were quantified using Design of Experiments (DoE) with 5 runs and 1 centerpoint. The selected objective and process model in this investigation are screening and interaction. Findings from this research show that to obtain particles larger than 35 nm, it is recommended to increase sodium citrate concentration, at low chloroauric acid values. These conditions will help to achieve smaller zeta potential, too.

Essay on the nucleoli survey by the α- and β-satellite DNA probes of the acrocentric chromosomes in mitogen-stimulated human lymphocytes

2000 ◽  
Vol 43 (2) ◽  
pp. 61-68 ◽  
Author(s):  
Halil Demirtas ◽  
Zühal Candemir ◽  
Nurhan Cücer ◽  
Nalan Imamoglu ◽  
Hamiyet Dönmez ◽  
...  
Keyword(s):  
Satellite Dna ◽  
Human Lymphocytes ◽  
Dna Probes ◽  
Acrocentric Chromosomes

Characteristics of TAA–ZnS Buffer Layer by Addition of Sodium Citrate for CIGS Thin Film Solar Cell

2020 ◽  
Vol 20 (11) ◽  
pp. 6659-6664
Author(s):  
Jeong Eun Park ◽  
So Mang Park ◽  
Eun Ji Bae ◽  
Donggun Lim
Keyword(s):  
Thin Film ◽  
Thin Films ◽  
Buffer Layer ◽  
Deposition Rate ◽  
Sodium Citrate ◽  
Wide Bandgap ◽  
Buffer Layers ◽  
Thin Film Solar Cells ◽  
Citrate Concentration ◽  
Cigs Thin Film

Zinc Sulfide (ZnS) is an environmentally friendly material with a wide bandgap (Eg = 3.7 eV) comparable to that of cadmium sulfide (CdS) (2.4 eV), which is conventionally used as buffer layer in Cu(In,Ga)Se2 (CIGS) thin film solar cells. Conventional ZnS buffer layers are manufactured using thiourea, and, these layers possess a disadvantage in that their deposition rate is lower than that of CdS buffer layers. In this paper, thioacetamide (TAA) was used as a sulfur precursor instead of thiourea to increase the deposition rate. However, the ZnS thin films deposited with TAA exhibited a higher roughness than the ZnS thin films deposited with thiourea. Sodium citrate was therefore added to increase the uniformity and decrease the roughness of the former ZnS thin films. When sodium citrate was used, the thin films demonstrated a high transmittance via the controlled generation of particles. In the case of TAA–ZnS thin films doped with a sodium citrate concentration of 0.04 M, the granules on the surface disappeared and these thin films were denser than the TAA–ZnS thin films deposited with a lower sodium citrate concentration. It is considered that the rate of the ion-by-ion reaction increased due to the addition of sodium citrate, thereby resulting in a uniform thin film. Consequently, TAA–ZnS thin films with thicknesses of approximately 40 nm and high transmittances of 83% were obtained when a sodium citrate concentration of 0.04 M was used.

Random single chromatid and nonrandom double chromatid type segregation of human acrocentric chromosomes in BrdU-labeled mitoses

1984 ◽  
Vol 26 (2) ◽  
pp. 137-140 ◽  
Author(s):  
S. Chemitiganti ◽  
R. S. Verma ◽  
S. Ved Brat ◽  
H. Dosik
Keyword(s):  
Human Leukocytes ◽  
Satellite Association ◽  
Chromatid Segregation ◽  
Random Segregation ◽  
Acrocentric Chromosomes ◽  
Single Chromatid ◽  
Cis And Trans

Chromatid segregation was analyzed using satellite association of 5-bromodeoxyuridine (BrdU) differentially stained acrocentric chromosomes of human leukocytes. Data were classified into cis and trans configurations in second and third division cycles. It was found that single chromatid types have random segregation (1:1) while nonrandom segregation was noted for double chromatid types. The nonrandom segregation hypothesis of earlier investigators needs to be reexamined.Key words: chromatid segregation, BrdU, mitosis, acrocentric.

The relationship between micronuclei in human lymphocytes and selected micronutrients in vegetarians and non-vegetarians

2006 ◽  
Vol 611 (1-2) ◽  
pp. 64-70 ◽  
Author(s):  
Alena Kažimírová ◽  
Magdaléna Barančoková ◽  
Marica Krajčovičová-Kudláčková ◽  
Katarína Volkovová ◽  
Marta Staruchová ◽  
...  
Keyword(s):  
Human Lymphocytes ◽  
The Relationship

scholarly journals The relationship between adrenaline and beta 2-adrenoceptors on human lymphocytes.

1990 ◽  
Vol 30 (S1) ◽  
pp. 145S-147S ◽  
Author(s):  
SJ Graafsma ◽  
MP Hectors ◽  
LJ Tits ◽  
JF Rodrigues de Miranda ◽  
T. Thien
Keyword(s):  
Human Lymphocytes ◽  
Beta 2 ◽  
The Relationship
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