RADIATION-INDUCED SPERMATOGONIAL EXCHANGE BETWEEN THE X AND Y CHROMOSOMES IN THE GUPPY

1969 ◽  
Vol 11 (4) ◽  
pp. 948-954 ◽  
Author(s):  
Johannes Horst Schröder
Keyword(s):  
Stem Cells ◽  
Germ Cells ◽  
Crossing Over ◽  
Male Germ Cells ◽  
Control Series ◽  
Exchange Frequency ◽  
Y Chromosomes ◽  
Radiation Induced ◽  
And Control ◽  
Lebistes Reticulatus

Irradiation of male germ cells in newborn guppies, Lebistes reticulatus Peters, causes an increase in the exchange frequency between the X and Y chromosomes in comparison to unirradiated controls among the offspring of the irradiated males, provided the offspring are produced during an interval between 161 and 283 days after irradiation. No significant increase of crossing-over frequency was obtained among offspring produced during the interval between 59 and 145 days after irradiation. It is concluded that irradiation of early spermatogonia (59 to 145 days postirradiation) does not increase the exchange frequency between sex chromosomes whereas the irradiation of stem cells (161 to 283 days) provides a significantly higher incidence of crossing-over events in the irradiated series than in the controls. The excess of crossovers in the irradiated stem cells of spermatogenesis is due only to clusters of crossovers in the irradiated series, since on excluding the clusters no difference between irradiated and control series could be found.

INHERITANCE OF RADIATION-INDUCED SPINAL CURVATURES IN THE GUPPY LEBISTES RETICULATUS

1969 ◽  
Vol 11 (4) ◽  
pp. 937-947 ◽  
Author(s):  
Johannes Horst Schröder
Keyword(s):  
Germ Cells ◽  
Vertebral Column ◽  
Segregation Ratio ◽  
X Rays ◽  
Wild Type ◽  
Spinal Curvatures ◽  
Recessive Mutations ◽  
Quantitative Characters ◽  
Radiation Induced ◽  
Lebistes Reticulatus

Hereditary changes in the shape of the vertebral column in Lebistes reticulatus appeared after ancestral irradiation of immature germ cells with 500 or 1000 R of X-rays. Although the mutant to wild-type ratios in the F2 generation after outcrossing fitted a digenic and a trigenic segregation ratio, respectively, the quantitative characters in question are assumed to be caused by recessive mutations of polygenes which are highly mutable.

217 PROTEIN-INDUCED TRANSDIFFERENTIATION INTO MALE GERM CELL-LIKE LINEAGE FROM CHICKEN FETAL BONE MARROW STEM CELLS

2012 ◽  
Vol 24 (1) ◽  
pp. 220
Author(s):  
J. M. Yoo ◽  
J. J. Park ◽  
K. Gobianand ◽  
J. Y. Ji ◽  
J. S. Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Retinoic Acid ◽  
Germ Cell ◽  
Germ Cells ◽  
Cultured Cells ◽  
Male Germ Cell ◽  
Male Germ Cells ◽  
Germ Cell Differentiation ◽  
Transgenic Chickens

Bone marrow (BM)-derived stem cells are capable of transdifferentiation into multilineage cells like muscle, bone, cartilage, fat and nerve cells. In this study, we investigated the capability of mesenchymal stem cells (MSC) derived from BM into germ cell differentiation in the chicken. Chicken MSCs were isolated from BM of day 20 fertilized fetal chicken with Ficoll-Paque Plus. Isolated cells were cultured in advance-DMEM (ADMEM) supplemented with 10% fetal bovine serum and antibiotics. Once confluent, cells were subcultured until five passages. The cultured cells showed fibroblast-like morphology. The cells had positive expressions of Oct4, Sox2 and Nanog. Two induction methods were conducted to examine the ability of transdifferentation into male germ cells. In group 1, MSC were cultured in ADMEM containing retinoic acid and chicken testicular extracts proteins for 10 to 15 days. In group 2, MSC were permeabilized by streptolysin O and treated with chicken testicular protein extracts. In both treatment groups, MSC were cultured in ADMEM containing retinoic acid for 10 to 15 days. We found that chicken MSC had a positive expression of pluripotent proteins such as Oct4, Sox2, Nanog and a small population of chicken MSC seem to transdifferentiate into male germ cell-like cells. These cells expressed early germ cell markers and male germ-cell-specific markers (Dazl, C-kit, Stra8 and DDX4) as analysed by reverse transcription-PCR and immunohistochemistry. These results demonstrated that chicken MSC may differentiate into male germ cells and the same might be used as a potential source of cells for production of transgenic chickens. This study was carried out with the support of Agenda Program (Project No. PJ0064692011), RDA and Republic of Korea.

scholarly journals THE USE OF SEMIPERMEABLE SPHERES ENGULFED WITH MOUSE EMBRYONIC STEM CELLS TO GENERATE MALE GERM CELLS

2020 ◽  
Vol 114 (3) ◽  
pp. e439
Author(s):  
Sherina Lawrence ◽  
Mounia Haddad ◽  
Philip Xie ◽  
Zev Rosenwaks ◽  
Gianpiero D. Palermo
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Male Germ Cells

Nanos2 promotes differentiation of chicken (Gallus gallus ) embryonic stem cells to male germ cells

2018 ◽  
Vol 119 (6) ◽  
pp. 4435-4446 ◽  
Author(s):  
Wenhui Zhang ◽  
Yulin Bi ◽  
Yingjie Wang ◽  
Dong Li ◽  
Nana He ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cells ◽  
Embryonic Stem ◽  
Gallus Gallus ◽  
Male Germ Cells

scholarly journals Generation of male germ cells from induced pluripotent stem cells (iPS cells): an in vitro and in vivo study

2012 ◽  
Vol 14 (4) ◽  
pp. 574-579 ◽  
Author(s):  
Yong Zhu ◽  
Hong-Liang Hu ◽  
Peng Li ◽  
Shi Yang ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Germ Cells ◽  
Pluripotent Stem Cells ◽  
Ips Cells ◽  
In Vivo Study ◽  
Male Germ Cells ◽  
Induced Pluripotent
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