Comparison of the efficacy of three concentrations of retinoic acid for transdifferentiation induction in sheep marrow-derived mesenchymal stem cells into male germ cells

Andrologia ◽  
2012 ◽  
Vol 46 (1) ◽  
pp. 24-35 ◽  
Author(s):  
M. Ghasemzadeh-Hasankolaei ◽  
M.B. Eslaminejad ◽  
R. Batavani ◽  
M. Sedighi-Gilani
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Retinoic Acid ◽  
Germ Cells ◽  
Male Germ Cells

217 PROTEIN-INDUCED TRANSDIFFERENTIATION INTO MALE GERM CELL-LIKE LINEAGE FROM CHICKEN FETAL BONE MARROW STEM CELLS

2012 ◽  
Vol 24 (1) ◽  
pp. 220
Author(s):  
J. M. Yoo ◽  
J. J. Park ◽  
K. Gobianand ◽  
J. Y. Ji ◽  
J. S. Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Retinoic Acid ◽  
Germ Cell ◽  
Germ Cells ◽  
Cultured Cells ◽  
Male Germ Cell ◽  
Male Germ Cells ◽  
Germ Cell Differentiation ◽  
Transgenic Chickens

Bone marrow (BM)-derived stem cells are capable of transdifferentiation into multilineage cells like muscle, bone, cartilage, fat and nerve cells. In this study, we investigated the capability of mesenchymal stem cells (MSC) derived from BM into germ cell differentiation in the chicken. Chicken MSCs were isolated from BM of day 20 fertilized fetal chicken with Ficoll-Paque Plus. Isolated cells were cultured in advance-DMEM (ADMEM) supplemented with 10% fetal bovine serum and antibiotics. Once confluent, cells were subcultured until five passages. The cultured cells showed fibroblast-like morphology. The cells had positive expressions of Oct4, Sox2 and Nanog. Two induction methods were conducted to examine the ability of transdifferentation into male germ cells. In group 1, MSC were cultured in ADMEM containing retinoic acid and chicken testicular extracts proteins for 10 to 15 days. In group 2, MSC were permeabilized by streptolysin O and treated with chicken testicular protein extracts. In both treatment groups, MSC were cultured in ADMEM containing retinoic acid for 10 to 15 days. We found that chicken MSC had a positive expression of pluripotent proteins such as Oct4, Sox2, Nanog and a small population of chicken MSC seem to transdifferentiate into male germ cell-like cells. These cells expressed early germ cell markers and male germ-cell-specific markers (Dazl, C-kit, Stra8 and DDX4) as analysed by reverse transcription-PCR and immunohistochemistry. These results demonstrated that chicken MSC may differentiate into male germ cells and the same might be used as a potential source of cells for production of transgenic chickens. This study was carried out with the support of Agenda Program (Project No. PJ0064692011), RDA and Republic of Korea.

Effect of Zinc Ions on Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells to Male Germ Cells and Some Germ Cell-Specific Gene Expression in Rams

2012 ◽  
Vol 150 (1-3) ◽  
pp. 137-146 ◽  
Author(s):  
Mohammad Ghasemzadeh-Hasankolai ◽  
Roozali Batavani ◽  
Mohamadreza Baghaban Eslaminejad ◽  
Mohammadali Sedighi-Gilani
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Germ Cell ◽  
Germ Cells ◽  
Specific Gene ◽  
Zinc Ions ◽  
Male Germ Cells ◽  
Specific Gene Expression

Retinoic acid and/or progesterone differentiate mouse induced pluripotent stem cells into male germ cells in vitro

2019 ◽  
Vol 121 (3) ◽  
pp. 2159-2169
Author(s):  
Javad Amini Mahabadi ◽  
Abolfazl Aazami Tameh ◽  
Sayyed Alireza Talaei ◽  
Mohammad Karimian ◽  
Tahereh Rahiminia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Induced Pluripotent Stem Cells ◽  
Germ Cells ◽  
Pluripotent Stem Cells ◽  
Male Germ Cells ◽  
Induced Pluripotent

scholarly journals Induction of goat bone marrow mesenchymal stem cells into putative male germ cells using mRNA for STRA8, BOULE and DAZL

2019 ◽  
Vol 71 (2) ◽  
pp. 563-572 ◽  
Author(s):  
Yan-Li Zhang ◽  
Pei-Zhen Li ◽  
Jing Pang ◽  
Yong-Jie Wan ◽  
Guo-Min Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Germ Cells ◽  
Male Germ Cells ◽  
Marrow Mesenchymal Stem Cells
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