CYTOGENETICS OF A BURLEY TOBACCO BREEDING LINE DERIVED FROM A CROSS WITH NICOTIANA LONGIFLORA CAV

1969 ◽  
Vol 11 (2) ◽  
pp. 382-388
Author(s):  
G. B. Collins ◽  
P. D. Legg
Keyword(s):  
Nicotiana Tabacum ◽  
Strong Evidence ◽  
Pollen Viability ◽  
Staining Technique ◽  
Breeding Line ◽  
Black Shank ◽  
Burley Tobacco ◽  
Nicotiana Tabacum L ◽  
Nicotiana Longiflora ◽  
Metaphase I

Cytological investigations of a burley tobacco (Nicotiana tabacum L.) breeding line designated as L-8 and carrying race 0 black shank resistance derived from a cross with N. longiflora Cav. revealed the regular occurrence of 24 bivalents at metaphase I of meiosis. Instability was apparent at the meiotic anaphase and tetrad stages and from observations of mitotic anaphase and metaphase figures. Pollen viability of L-8 as estimated by a staining technique was very similar to that obtained for a standard burley variety. Comparable cytological observations were extended to the F1 hybrids of L-8 with three burley varieties. The findings were similar for L-8 and the F1 hybrids. The occurrence of 24 bivalents in meiocytes of F1 hybrids provided strong evidence for incorporation of black shank resistance from N. longiflora into N. tabacum by segmental substitution rather than by alien addition of a longiflora chromosome as previously reported.

INHERITANCE OF RESISTANCE TO BLACK SHANK IN NICOTIANA TABACUM L.

1971 ◽  
Vol 13 (3) ◽  
pp. 422-428 ◽  
Author(s):  
G. B. Collins ◽  
Paul D. Legg ◽  
C. C. Litton ◽  
M. J. Kasperbauer
Keyword(s):  
Nicotiana Tabacum ◽  
Dominant Gene ◽  
Inheritance Of Resistance ◽  
Breeding Line ◽  
Single Dominant Gene ◽  
Black Shank ◽  
Resistant Genotype ◽  
Burley Tobacco ◽  
Nicotiana Tabacum L ◽  
Resistant Progeny

Resistance to race 0 of black shank (Phytophthora parasitica var. Nicotianae) derived from Nicotians longiflora Cav. and transferred to the burley tobacco breeding line L8 appears to be conditioned by a single dominant gene. However, on the basis of expected ratios for a single dominant gene, a deficiency of resistant progeny was observed in the F2 and backcross generations. Haploid plants extracted from F1 individuals heterozygous for black shank resistance substantiated the deficiency in the resistance class. The haploid segregation with reduced recovery of the resistant genotype rules out preferential pollination as the cause for the altered ratios. The mechanism responsible for impaired function of the gamete carrying the allele for resistance is not known.

scholarly journals Rootstock-Scion Interaction Affects the Composition and Pathogen Inhibitory Activity of Tobacco (Nicotiana tabacum L.) Root Exudates

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1652
Author(s):  
Cheng-Sheng Zhang ◽  
Yanfen Zheng ◽  
Lijuan Peng ◽  
Jianmin Cao
Keyword(s):  
Nicotiana Tabacum ◽  
Root Exudates ◽  
Root Exudate ◽  
Phytophthora Nicotianae ◽  
Susceptible Cultivar ◽  
Black Shank ◽  
Nicotiana Tabacum L ◽  
Disease Induction ◽  
Resistant Rootstock ◽  
Ethylhexyl Phthalate

The composition and allelopathy to Phytophthora nicotianae (the causal agent of tobacco black shank disease) of root exudates from a resistant tobacco (Nicotiana tabacum L.) cultivar Gexin 3, a susceptible cultivar Xiaohuangjin 1025 and their reciprocal grafts were investigated. Grafting with disease-resistant rootstock could improve resistance to black shank; this is closely related to the allelopathy of root exudates. The root exudates from the resistant cultivar inhibited the growth of P. nicotianae, while those from the susceptible cultivar promoted the growth; the grafting varieties had intermediate properties. The root exudate composition differed among cultivars. Gexin 3 was rich in esters and fatty acids, while Xiaohuangjin 1025 contained more hydrocarbons and phenolic acids. The composition of root exudates of grafted cultivars as well as their allelopathy to P. nicotianae were altered, and tended to be close to the composition of cultivar used as rootstock. Eugenol, 4-tert-butylphenol, mono (2-ethylhexyl) phthalate, 4-hydroxybenzoic acid, 2,6-di-tert-butylphenol, dipropyl phthalate, and methyl myristate were identified as the main compounds contributing to inhibitory properties of root exudates. Sorbitol was suggested to play a role in disease induction. Overall, rootstock–scion interaction affected the composition of tobacco root exudates, which may be attributed to the different disease resistance among grafted plants, rootstock and scion.

Expression of a Nicotiana tabacum pathogen-induced gene is involved in the susceptibility to black shank

2016 ◽  
Vol 43 (6) ◽  
pp. 534 ◽  
Author(s):  
Roxana Portieles ◽  
Eduardo Canales ◽  
Osmani Chacon ◽  
Yussuan Silva ◽  
Ingrid Hernández ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Compatible Interaction ◽  
Virus Induced Gene Silencing ◽  
Black Shank ◽  
Pathogen Virulence ◽  
Resistant Species ◽  
Nicotiana Tabacum L ◽  
Oomycete Pathogen ◽  
Plant Pathogen Interactions ◽  
Host Genes

Many host genes induced during compatible plant–pathogen interactions constitute targets of pathogen virulence factors that act to suppress host defenses. In order to identify Nicotiana tabacum L. genes for pathogen-induced proteins involved in susceptibility to the oomycete Phytophthora parasitica var. nicotianae, we used SuperSAGE technology combined with next-generation sequencing to identify transcripts that were differentially upregulated during a compatible interaction. We identified a pathogen-induced gene (NtPIP) that was rapidly induced only during the compatible interaction. Virus-induced gene silencing of NtPIP reduced the susceptibility of N. tabacum to P. parasitica var. nicotianae. Additionally, transient expression of NtPIP in the resistant species Nicotiana megalosiphon Van Heurck & Mull. Arg. compromised the resistance to P. parasitica var. nicotianae. This pathogen-induced protein is therefore a positive regulator of the susceptibility response against an oomycete pathogen in tobacco.

RESPONSE OF CIGAR TOBACCO TO DIFFERENT DATES OF PLANTING AND SPACING

1975 ◽  
Vol 55 (1) ◽  
pp. 303-308
Author(s):  
V. KOZUMPLIK ◽  
P. P. LUKOSEVICIUS
Keyword(s):  
Nicotiana Tabacum ◽  
Chemical Characteristics ◽  
Breeding Line ◽  
Total Alkaloids ◽  
Nicotiana Tabacum L ◽  
Leaf Yield ◽  
Number Of Leaves ◽  
Planting Distance ◽  
Grade Index

Response of cigar tobacco (Nicotiana tabacum L.) in morphological, agronomic and chemical characteristics to date of planting and planting distance was studied during 1971–73. A cultivar and a breeding line of cigar tobacco were transplanted outdoors on 26 May, 9 (or 12) June and 23 June. The tobacco was spaced 36, 41 and 46 cm in rows that were 97 cm apart. The best results were obtained when cigar tobacco was transplanted not later than 9 June, and the plants were spaced 41 cm in the rows that were 97 cm apart. The highest number of leaves per plant, cured-leaf yield, percentage of nicotine and total alkaloids were obtained from the tobacco transplanted on the first or second date in 3 yr. In 2 yr, the later tobacco had the tallest plants, the largest leaves, the highest percent filler leaf, grade and crop index. Earlier-transplanted tobacco was harvested earlier, although the period to flower decreased with later date of planting. On the average, the tobacco spaced 41 cm was taller and had a shorter period to flower, and higher yield and crop index than that spaced 36 cm or 46 cm. Grade index, percent filler leaf, nicotine and total alkaloids were affected significantly by the distance in 1 yr only.

Sign in / Sign up

Export Citation Format

Share Document