Expression of a Nicotiana tabacum pathogen-induced gene is involved in the susceptibility to black shank

2016 ◽  
Vol 43 (6) ◽  
pp. 534 ◽  
Author(s):  
Roxana Portieles ◽  
Eduardo Canales ◽  
Osmani Chacon ◽  
Yussuan Silva ◽  
Ingrid Hernández ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Compatible Interaction ◽  
Virus Induced Gene Silencing ◽  
Black Shank ◽  
Pathogen Virulence ◽  
Resistant Species ◽  
Nicotiana Tabacum L ◽  
Oomycete Pathogen ◽  
Plant Pathogen Interactions ◽  
Host Genes

Many host genes induced during compatible plant–pathogen interactions constitute targets of pathogen virulence factors that act to suppress host defenses. In order to identify Nicotiana tabacum L. genes for pathogen-induced proteins involved in susceptibility to the oomycete Phytophthora parasitica var. nicotianae, we used SuperSAGE technology combined with next-generation sequencing to identify transcripts that were differentially upregulated during a compatible interaction. We identified a pathogen-induced gene (NtPIP) that was rapidly induced only during the compatible interaction. Virus-induced gene silencing of NtPIP reduced the susceptibility of N. tabacum to P. parasitica var. nicotianae. Additionally, transient expression of NtPIP in the resistant species Nicotiana megalosiphon Van Heurck & Mull. Arg. compromised the resistance to P. parasitica var. nicotianae. This pathogen-induced protein is therefore a positive regulator of the susceptibility response against an oomycete pathogen in tobacco.

scholarly journals Rootstock-Scion Interaction Affects the Composition and Pathogen Inhibitory Activity of Tobacco (Nicotiana tabacum L.) Root Exudates

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1652
Author(s):  
Cheng-Sheng Zhang ◽  
Yanfen Zheng ◽  
Lijuan Peng ◽  
Jianmin Cao
Keyword(s):  
Nicotiana Tabacum ◽  
Root Exudates ◽  
Root Exudate ◽  
Phytophthora Nicotianae ◽  
Susceptible Cultivar ◽  
Black Shank ◽  
Nicotiana Tabacum L ◽  
Disease Induction ◽  
Resistant Rootstock ◽  
Ethylhexyl Phthalate

The composition and allelopathy to Phytophthora nicotianae (the causal agent of tobacco black shank disease) of root exudates from a resistant tobacco (Nicotiana tabacum L.) cultivar Gexin 3, a susceptible cultivar Xiaohuangjin 1025 and their reciprocal grafts were investigated. Grafting with disease-resistant rootstock could improve resistance to black shank; this is closely related to the allelopathy of root exudates. The root exudates from the resistant cultivar inhibited the growth of P. nicotianae, while those from the susceptible cultivar promoted the growth; the grafting varieties had intermediate properties. The root exudate composition differed among cultivars. Gexin 3 was rich in esters and fatty acids, while Xiaohuangjin 1025 contained more hydrocarbons and phenolic acids. The composition of root exudates of grafted cultivars as well as their allelopathy to P. nicotianae were altered, and tended to be close to the composition of cultivar used as rootstock. Eugenol, 4-tert-butylphenol, mono (2-ethylhexyl) phthalate, 4-hydroxybenzoic acid, 2,6-di-tert-butylphenol, dipropyl phthalate, and methyl myristate were identified as the main compounds contributing to inhibitory properties of root exudates. Sorbitol was suggested to play a role in disease induction. Overall, rootstock–scion interaction affected the composition of tobacco root exudates, which may be attributed to the different disease resistance among grafted plants, rootstock and scion.

scholarly journals Identification of stably expressed QTL for resistance to black shank disease in tobacco (Nicotiana tabacum L.) line Beinhart 1000-1

2018 ◽  
Vol 6 (3) ◽  
pp. 282-290 ◽  
Author(s):  
Yusheng Zhang ◽  
Xuan Guo ◽  
Xingxing Yan ◽  
Min Ren ◽  
Caihong Jiang ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Black Shank ◽  
Nicotiana Tabacum L

INHERITANCE OF RESISTANCE TO BLACK SHANK IN NICOTIANA TABACUM L.

1971 ◽  
Vol 13 (3) ◽  
pp. 422-428 ◽  
Author(s):  
G. B. Collins ◽  
Paul D. Legg ◽  
C. C. Litton ◽  
M. J. Kasperbauer
Keyword(s):  
Nicotiana Tabacum ◽  
Dominant Gene ◽  
Inheritance Of Resistance ◽  
Breeding Line ◽  
Single Dominant Gene ◽  
Black Shank ◽  
Resistant Genotype ◽  
Burley Tobacco ◽  
Nicotiana Tabacum L ◽  
Resistant Progeny

Resistance to race 0 of black shank (Phytophthora parasitica var. Nicotianae) derived from Nicotians longiflora Cav. and transferred to the burley tobacco breeding line L8 appears to be conditioned by a single dominant gene. However, on the basis of expected ratios for a single dominant gene, a deficiency of resistant progeny was observed in the F2 and backcross generations. Haploid plants extracted from F1 individuals heterozygous for black shank resistance substantiated the deficiency in the resistance class. The haploid segregation with reduced recovery of the resistant genotype rules out preferential pollination as the cause for the altered ratios. The mechanism responsible for impaired function of the gamete carrying the allele for resistance is not known.

scholarly journals Gene Profiling of a Compatible Interaction Between Phytophthora infestans and Solanum tuberosum Suggests a Role for Carbonic Anhydrase

2005 ◽  
Vol 18 (9) ◽  
pp. 913-922 ◽  
Author(s):  
S. Restrepo ◽  
K. L. Myers ◽  
O. del Pozo ◽  
G. B. Martin ◽  
A. L. Hart ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Phytophthora Infestans ◽  
Polymerase Chain Reaction Analysis ◽  
Gene Profiling ◽  
Differentially Expressed ◽  
Cdna Clones ◽  
Compatible Interaction ◽  
Virus Induced Gene Silencing ◽  
Oomycete Pathogen ◽  
Late Blight Of Potato

Late blight of potato, caused by the oomycete pathogen Phytophthora infestans, is a devastating disease that can cause the rapid death of plants. To investigate the molecular basis of this compatible interaction, potato cDNA microarrays were utilized to identify genes that were differentially expressed in the host during a compatible interaction with P. infestans. Of the 7,680 cDNA clones represented on the array, 643 (12.9%) were differentially expressed in infected plants as compared with mock-inoculated control plants. These genes were classified into eight groups using a nonhierarchical clustering method with two clusters (358 genes) generally down-regulated, three clusters (241 genes) generally up-regulated, and three clusters (44 genes) with a significant change in expression at only one timepoint. Three genes derived from two down-regulated clusters were evaluated further, using reverse transcription real-time polymerase chain reaction analysis. For these analyses, both incompatible and compatible interactions were included to determine if suppression of these genes was specific to compatibility. One gene, plastidic carbonic anhydrase (CA), was found to have a very different expression pattern in compatible vs. incompatible interactions. Virus-induced gene silencing was used to suppress expression of this gene in Nicotiana benthamiana. In CA-silenced plants, the pathogen grew more quickly, indicating that suppression of CA increases susceptibility to P. infestans.

CYTOGENETICS OF A BURLEY TOBACCO BREEDING LINE DERIVED FROM A CROSS WITH NICOTIANA LONGIFLORA CAV

1969 ◽  
Vol 11 (2) ◽  
pp. 382-388
Author(s):  
G. B. Collins ◽  
P. D. Legg
Keyword(s):  
Nicotiana Tabacum ◽  
Strong Evidence ◽  
Pollen Viability ◽  
Staining Technique ◽  
Breeding Line ◽  
Black Shank ◽  
Burley Tobacco ◽  
Nicotiana Tabacum L ◽  
Nicotiana Longiflora ◽  
Metaphase I

Cytological investigations of a burley tobacco (Nicotiana tabacum L.) breeding line designated as L-8 and carrying race 0 black shank resistance derived from a cross with N. longiflora Cav. revealed the regular occurrence of 24 bivalents at metaphase I of meiosis. Instability was apparent at the meiotic anaphase and tetrad stages and from observations of mitotic anaphase and metaphase figures. Pollen viability of L-8 as estimated by a staining technique was very similar to that obtained for a standard burley variety. Comparable cytological observations were extended to the F1 hybrids of L-8 with three burley varieties. The findings were similar for L-8 and the F1 hybrids. The occurrence of 24 bivalents in meiocytes of F1 hybrids provided strong evidence for incorporation of black shank resistance from N. longiflora into N. tabacum by segmental substitution rather than by alien addition of a longiflora chromosome as previously reported.

Tobacco Chemistry/Chemische Zusammensetzung des Tabaks

1976 ◽  
Vol 8 (6) ◽  
Author(s):  
Arne J. Aasen ◽  
Sven-Olof Almquist ◽  
Curt R. Enzell
Keyword(s):  
Nicotiana Tabacum ◽  
Nicotiana Tabacum L

Abstract35: two isomeric 5,6-Epoxy-3-hydroxy-7-megastigmen-9-ones from Nicotiana tabacum L.

Inheritance of the Conversion of Nicotine to Nornicotine in Varieties of Nicotiana tabacum L. and Related Amphiploids 1

Crop Science ◽  
1964 ◽  
Vol 4 (4) ◽  
pp. 349-353 ◽  
Author(s):  
T. J. Mann ◽  
J. A. Weybrew ◽  
D. F. Matzinger ◽  
J. L. Hall
Keyword(s):  
Nicotiana Tabacum ◽  
Nicotiana Tabacum L
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