Discovery of a new source of resistance to Fusarium oxysporum, cause of Fusarium wilt in Allium fistulosum, located on chromosome 2 of Allium cepa Aggregatum group

Genome ◽  
2012 ◽  
Vol 55 (11) ◽  
pp. 797-807 ◽  
Author(s):  
Hoa Q. Vu ◽  
Magdi A. El-Sayed ◽  
Shin-Ichi Ito ◽  
Naoki Yamauchi ◽  
Masayoshi Shigyo
Keyword(s):  
Fusarium Oxysporum ◽  
Allium Cepa ◽  
Fusarium Wilt ◽  
Root Exudates ◽  
Allium Fistulosum ◽  
Addition Lines ◽  
Wilt Resistance ◽  
Monosomic Addition ◽  
Monosomic Addition Lines ◽  
Fusarium Wilt Resistance

This study was carried out to evaluate the antifungal effect of Allium cepa Aggregatum group (shallot) metabolites on Fusarium oxysporum and to determine the shallot chromosome(s) related to Fusarium wilt resistance using a complete set of eight Allium fistulosum – shallot monosomic addition lines. The antifungal effects of hexane, butanol, and water extraction fractions from bulbs of shallot on 35 isolates of F. oxysporum were examined using the disc diffusion method. Only hexane and butanol fractions showed high antifungal activity. Shallot showed no symptom of disease after inoculation with F. oxysporum f. sp. cepae. The phenolic content of the roots and the saponin content of root exudates of inoculated shallot increased to much higher levels than those of the control at 3 days after inoculation. Application of freeze-dried shallot root exudates to seeds of A. fistulosum soaked in a spore suspension of F. oxysporum resulted in protection of seedlings against infection. Among eight monosomic addition lines and A. fistulosum, FF+2A showed the highest resistance to Fusarium wilt. This monosomic addition line also showed a specific saponin band derived from shallot on the thin layer chromatography profile of saponins in the eight monosomic addition lines. The chromosome 2A of shallot might possess some of the genes related to Fusarium wilt resistance.

scholarly journals Natural Variation in Portuguese Common Bean Germplasm Reveals New Sources of Resistance Against Fusarium oxysporum f. sp. phaseoli and Resistance-Associated Candidate Genes

2020 ◽  
Vol 110 (3) ◽  
pp. 633-647 ◽  
Author(s):  
Susana T. Leitão ◽  
Marcos Malosetti ◽  
Qijan Song ◽  
Fred van Eeuwijk ◽  
Diego Rubiales ◽  
...  
Keyword(s):  
Common Bean ◽  
Fusarium Oxysporum ◽  
Candidate Genes ◽  
Fusarium Wilt ◽  
Functional Markers ◽  
Gene Pools ◽  
Primary Metabolism ◽  
Sources Of Resistance ◽  
Wilt Resistance ◽  
Fusarium Wilt Resistance

Common bean (Phaseolus vulgaris) is one of the most consumed legume crops in the world, and Fusarium wilt, caused by the fungus Fusarium oxysporum f. sp. phaseoli, is one of the major diseases affecting its production. Portugal holds a very promising common bean germplasm with an admixed genetic background that may reveal novel genetic resistance combinations between the original Andean and Mesoamerican gene pools. To identify new sources of Fusarium wilt resistance and detect resistance-associated single-nucleotide polymorphisms (SNPs), we explored, for the first time, a diverse collection of the underused Portuguese common bean germplasm by using genome-wide association analyses. The collection was evaluated for Fusarium wilt resistance under growth chamber conditions, with the highly virulent F. oxysporum f. sp. phaseoli strain FOP-SP1 race 6. Fourteen of the 162 Portuguese accessions evaluated were highly resistant and 71 intermediate. The same collection was genotyped with DNA sequencing arrays, and SNP–resistance associations were tested via a mixed linear model accounting for the genetic relatedness between accessions. The results from the association mapping revealed nine SNPs associated with resistance on chromosomes Pv04, Pv05, Pv07, and Pv08, indicating that Fusarium wilt resistance is under oligogenic control. Putative candidate genes related to phytoalexin biosynthesis, hypersensitive response, and plant primary metabolism were identified. The results reported here highlight the importance of exploring underused germplasm for new sources of resistance and provide new genomic targets for the development of functional markers to support selection in future disease resistance breeding programs.

AFLP linkage group assignment to the chromosomes of Allium cepa L. via monosomic addition lines

2000 ◽  
Vol 100 (3-4) ◽  
pp. 480-486 ◽  
Author(s):  
A. W. van Heusden ◽  
M. Shigyo ◽  
Y. Tashiro ◽  
R. Vrielink-van Ginkel ◽  
C. Kik
Keyword(s):  
Linkage Group ◽  
Allium Cepa ◽  
Addition Lines ◽  
Group Assignment ◽  
Allium Cepa L ◽  
Monosomic Addition ◽  
Monosomic Addition Lines

Transmission of alien chromosomes from selfed progenies of a complete set of Allium monosomic additions: the development of a reliable method for the maintenance of a monosomic addition set

Genome ◽  
2003 ◽  
Vol 46 (6) ◽  
pp. 1098-1103 ◽  
Author(s):  
M Shigyo ◽  
T Wako ◽  
A Kojima ◽  
N Yamauchi ◽  
Y Tashiro
Keyword(s):  
Allium Cepa ◽  
Transmission Rate ◽  
Allium Fistulosum ◽  
Selfed Progeny ◽  
Homologous Chromosomes ◽  
Monosomic Additions ◽  
Glutamate Dehydrogenase Activity ◽  
Monosomic Addition ◽  
Complete Set ◽  
Monosomic Addition Lines

Selfed progeny of a complete set of Allium fistulosum – Allium cepa monosomic addition lines (2n = 2x + 1 = 17, FF+1A–FF+8A) were produced to examine the transmission rates of respective alien chromosomes. All eight types of the selfed monosomic additions set germinable seeds. The numbers of chromosomes (2n) in the seedlings were 16, 17, or 18. The eight extra chromosomes varied in transmission rate (%) from 9 (FF+2A) to 49 (FF+8A). The complete set of monosomic additions was reproduced successfully by self-pollination. A reliable way to maintain a set of Allium monosomic additions was developed using a combination of two crossing methods, selfing and female transmission. FF+8A produced two seedlings with 18 chromosomes. Cytogenetical analyses, including GISH, showed that the seedlings were disomic addition plants carrying two entire homologous chromosomes from A. cepa in an integral diploid background of A. fistulosum. Flow cytometry analysis showed that a double dose of the alien 8A chromosome caused fluorescence intensity values spurring in DNA content, and isozyme analysis showed increased glutamate dehydrogenase activity at the gene locus Gdh-1.Key words: Allium cepa, Allium fistulosum, monosomic addition, disomic addition, transmission rate.

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