Integrated cytogenetic, ecological, and DNA barcode study reveals cryptic diversity in Simulium (Gomphostilbia) angulistylum (Diptera: Simuliidae)

Genome ◽  
2012 ◽  
Vol 55 (6) ◽  
pp. 447-458 ◽  
Author(s):  
Pairot Pramual ◽  
Chaliow Kuvangkadilok
Keyword(s):  
Mitochondrial Dna ◽  
Sea Level ◽  
Dna Sequences ◽  
Demographic History ◽  
Dna Barcode ◽  
Integrated Approach ◽  
Genetic Structuring ◽  
Population Genetic Analysis ◽  
Black Fly ◽  
Population Demographic

An integrated approach based on cytogenetics, molecular genetics, and ecology was used to examine diversity in the black fly Simulium angulistylum Takaoka & Davies in Thailand. Cytological analysis revealed three cytoforms (A, B, and C) of S. angulistylum differentiated by fixed chromosome inversions. Distributions of these cytoforms were associated with ecology. Cytoforms A and B were found in low-altitude habitats (<600 m above sea level), whereas cytoform C occurred at high altitudes (>1000 m above sea level). Mitochondrial DNA sequences of the cytochrome oxidase subunit I barcoding region revealed significant genetic differentiation among the cytoforms. The mitochondrial DNA haplotype network revealed divergent lineages within cytoforms, indicating additional hidden diversity. Therefore, integrated approaches are necessary for fully understanding black fly biodiversity. Population genetic analysis revealed high genetic structuring that could be due to the habitat preferences of S. angulistylum. Phylogeographic analyses indicated population demographic expansion at the mid-Pleistocene (900 000 years ago), which is older than for other black flies and insects in the Southeast Asian mainland. The high level of genetic structure and diversity, therefore, could also be due to the long demographic history of S. angulistylum.

The mitochondrial DNA haplotypes of snails of the estuarine hydrobiid genus Tatea cross species and biogeographic boundaries

2009 ◽  
Vol 60 (8) ◽  
pp. 861 ◽  
Author(s):  
D. J. Colgan ◽  
P. da Costa
Keyword(s):  
Mitochondrial Dna ◽  
Dna Sequences ◽  
Marine Species ◽  
Habitat Diversity ◽  
12S Rrna ◽  
Genetic Structuring ◽  
Dna Variation ◽  
Eastern Australia ◽  
Mitochondrial 12S Rrna ◽  
Marine Littoral

Investigations of estuarine taxa can provide a perspective on phylogeography that complements studies of marine littoral organisms. For example, reductions in gene flow between populations and increased genetic structuring would be expected in estuarine species. The substantial amount of information about marine species and the habitat diversity along long latitudinal spans makes south-eastern Australia an excellent potential location for comparing marine and estuarine taxa. To investigate this potential, we studied the phylogeography of the two species in the estuarine gastropod genus Tatea. These have extensive and broadly overlapping distributions that encompass known marine phylogeographic boundaries. Against expectation, both Tatea species showed a remarkable lack of geographic and inter-specific variability in mitochondrial 12S rRNA (107 specimens) and cytochrome c oxidase subunit I (39) DNA sequences. No major phylogeographic discontinuities were revealed in either species and there was minimal haplotype divergence between them for either 12S rRNA or COI. The patterns of mitochondrial DNA variation discovered in Tatea may be due to a recent selective sweep or range expansion from a population in which there was little variability. Both possibilities are complicated by having to explain the similarity of the patterns in the two species.

scholarly journals Population structure and demographic history of Pacific herring Clupea pallasii around Japan inferred from mitochondrial DNA sequences

2017 ◽  
Vol 83 (2) ◽  
pp. 163-173 ◽  
Author(s):  
TOMONARI FUJITA ◽  
SHUICHI KITADA ◽  
YASUKO HARADA ◽  
YUKINO ISHIDA ◽  
SHOKO SANO ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Population Structure ◽  
Dna Sequences ◽  
Demographic History ◽  
Pacific Herring ◽  
Clupea Pallasii ◽  
History Of

Phylogeography of the black fly Simulium aureohirtum (Diptera: Simuliidae) in Thailand

Genome ◽  
2014 ◽  
Vol 57 (8) ◽  
pp. 411-417 ◽  
Author(s):  
Jiraporn Thaijarern ◽  
Piyamas Nanork Sopaladawan ◽  
Komgrit Wongpakam ◽  
Pairot Pramual
Keyword(s):  
Genetic Structure ◽  
Demographic History ◽  
High Genetic Diversity ◽  
Black Fly ◽  
History Of ◽  
Living Organisms ◽  
High Level ◽  
Genetic Structure And Diversity ◽  
Lineage Divergence ◽  
Population Demographic

The black fly Simulium aureohirtum Brunetti (Diptera: Simuliidae) is geographically widespread and utilizes diverse habitats. Previous studies revealed high genetic diversity, suggesting that this species could be a species complex. In this study, we used mitochondrial cytochrome c oxidase II sequences to examine genetic structure and demographic history of S. aureohirtum in Thailand. High level of genetic variation was observed due to the existence of genetically divergent lineages (A, B, and C). Lineages A and C were geographically widespread, while lineage B was geographically restricted to the eastern part of Thailand. The geographic localization of lineage B suggested limitations in gene flow, which is most likely a result of ecologically different habitats. Large genetic differences between individuals of lineages A and C, but geographically sympatric distributions of the members of these lineages, are considered to represent an admixture of the populations that have evolved allopatrically. Historical environmental change during the Pleistocene glaciations is possibly the factor that drove the lineage divergence. Population demographic history analyses revealed recent population expansions in the three lineages dating back to the last glaciations. These observations further highlight the significance of the Pleistocene climatic change on current genetic structure and diversity of living organisms of the Southeast Asian mainland.

scholarly journals Mitochondrial DNA sequence variations in populations of Sardinapilchardus(Walbaum 1792) along the Tunisian coasts

2019 ◽  
Vol 20 (2) ◽  
Author(s):  
ΚARIMA FADHLAOUI-ZID
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Genetic Structure ◽  
Dna Sequences ◽  
Population Distribution ◽  
Genetic Structuring ◽  
Efficient Management ◽  
Sequence Variations ◽  
Haplotypic Diversity ◽  
High Level

Sardine is a fish species of great economic importance to Tunisia. Knowledge of genetic diversity and population distribution is essential for an efficient management and sustainability of any regional fishery. This study aimed to assessing the genetic structure and to specify the stocks of the European sardine (Sardina pilchardus). To this end, 83 specimens were collected from three localities along the Tunisian coast and analysed using mitochondrial DNA sequences. The results of sequence analysis determined the existence of variations in 40 single nucleotide sites within the 307 bp fragment of the cytb gene examined and defined twenty different haplotypes. Genetic diversity, estimated by haplotypic diversity, was high in all samples. Tunisian S.pilchardus samples show some level of genetic structuring. First, genetic differentiation between localities (ΦST estimates) was significant for all comparison. Second, the analysis of molecular variance AMOVA indicates a high level of genetic variation (ΦST = 0.093; P<0.001). The structural patterns identified can be explained largely in relation to the regional oceanographic features. In conclusion, this study provided initial genetic data in making inference of the genetic structure of S. pilchardus along the Tunisian coasts. 

scholarly journals Population structure and demographic history of Pacific herring Clupea pallasii around Japan inferred from mitochondrial DNA sequences

2018 ◽  
Vol 84 (4) ◽  
pp. 585-585
Author(s):  
TOMONARI FUJITA ◽  
SHUICHI KITADA ◽  
YASUKO HARADA ◽  
YUKINO ISHIDA ◽  
SHOKO SANO ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Population Structure ◽  
Dna Sequences ◽  
Demographic History ◽  
Pacific Herring ◽  
Clupea Pallasii ◽  
History Of
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