scholarly journals Mitochondrial DNA sequence variations in populations of Sardinapilchardus(Walbaum 1792) along the Tunisian coasts

2019 ◽  
Vol 20 (2) ◽  
Author(s):  
ΚARIMA FADHLAOUI-ZID
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Genetic Structure ◽  
Dna Sequences ◽  
Population Distribution ◽  
Genetic Structuring ◽  
Efficient Management ◽  
Sequence Variations ◽  
Haplotypic Diversity ◽  
High Level

Sardine is a fish species of great economic importance to Tunisia. Knowledge of genetic diversity and population distribution is essential for an efficient management and sustainability of any regional fishery. This study aimed to assessing the genetic structure and to specify the stocks of the European sardine (Sardina pilchardus). To this end, 83 specimens were collected from three localities along the Tunisian coast and analysed using mitochondrial DNA sequences. The results of sequence analysis determined the existence of variations in 40 single nucleotide sites within the 307 bp fragment of the cytb gene examined and defined twenty different haplotypes. Genetic diversity, estimated by haplotypic diversity, was high in all samples. Tunisian S.pilchardus samples show some level of genetic structuring. First, genetic differentiation between localities (ΦST estimates) was significant for all comparison. Second, the analysis of molecular variance AMOVA indicates a high level of genetic variation (ΦST = 0.093; P<0.001). The structural patterns identified can be explained largely in relation to the regional oceanographic features. In conclusion, this study provided initial genetic data in making inference of the genetic structure of S. pilchardus along the Tunisian coasts. 

scholarly journals High levels of genetic diversity and an absence of genetic structure among breeding populations of the endangered Rufous-backed Bunting in Inner Mongolia, China: implications for conservation

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Shi Li ◽  
Dan Li ◽  
Lishi Zhang ◽  
Weiping Shang ◽  
Bo Qin ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Genetic Structure ◽  
Endangered Species ◽  
Habitat Loss ◽  
Inner Mongolia ◽  
Avian Species ◽  
Genetic Structuring ◽  
Breeding Populations ◽  
The Impact

Abstract Background The Rufous-backed Bunting, Emberiza jankowskii, is an endangered species that is primarily distributed in Inner Mongolia, China. The main threats to the continued persistence of this species are habitat loss and degradation. However, the impact of population loss on genetic diversity remains unclear. To support future conservation and management efforts, we assessed the genetic diversity and population structure of E. jankowskii using mitochondrial DNA and microsatellites. Methods Blood samples were collected from 7‒8-day-old nestlings in Inner Mongolia, China between May and August of 2012 and 2013. Mitochondrial DNA sequences and microsatellite markers were used to assess the genetic diversity, genetic structure and inbreeding of E. jankowskii. The results of genetic diversity and inbreeding were compared to other avian species. Results We found an unexpectedly high level of genetic diversity in terms of mitochondrial DNA and microsatellite compared to other avian species. However, there were high levels of gene flow and minimal genetic structuring, among the fragmented breeding populations of E. jankowskii in Inner Mongolia. These findings suggest that E. jankowskii in Inner Mongolia is a metapopulation. Despite the high genetic diversity of E. jankowskii, local populations in each small patch remain at risk of extinction due to habitat loss. In addition, the E. jankowskii population has a high risk of inbreeding. Conclusions To minimize further loss of genetic diversity of this endangered species, we suggest that the E. jankowskii in Inner Mongolia should be considered as a protected species for management purposes. Conservation efforts should concentrate on E. jankowskii habitat management. This may be most effectively achieved by protecting the current breeding habitats and prohibiting over-grazing.

Geographic Origin and Genetic Structure of Introduced Sika Deer, Kerama Deer (Cervus nippon keramae) on Ryukyus Inferred from Mitochondrial DNA Sequences

Mammal Study ◽  
2015 ◽  
Vol 40 (3) ◽  
pp. 187-192 ◽  
Author(s):  
Asuka Yamashiro ◽  
Akira Endo ◽  
Hidehiko Kuwataka ◽  
Yuki Matsumoto ◽  
Tadashi Yamashiro
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Structure ◽  
Dna Sequences ◽  
Sika Deer ◽  
Geographic Origin ◽  
Cervus Nippon

scholarly journals GENOMIC DETERMINATION OF THE MOST IMPORTANT FATHER LINES OF SLOVAK PINZGAU COWS

AGROFOR ◽  
2016 ◽  
Vol 1 (3) ◽  
Author(s):  
Veronika KUKUČKOVÁ ◽  
Nina MORAVČÍKOVÁ ◽  
Radovan KASARDA
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
The State ◽  
Most Probable Number ◽  
Pedigree Data ◽  
Probable Number ◽  
Expected Heterozygosity ◽  
High Level ◽  
The Bayesian Approach

The aim of this study was to assess genetic structure of Slovak Pinzgau populationbased on polymorphism at molecular markers using statistical methods. Femaleoffspring of 12 most frequently used bulls in Slovak Pinzgau breeding programmewere investigated. Pinzgau cattle were found to have a high level of diversity,supported by the number of alleles observed across loci (average 5.31, range 2-11)and by the high within-breed expected heterozygosity (average 0.66, range 0.64-0.73). The state of genetic diversity is satisfying and standard for local populations.Detection of 12 possible subpopulation structures provided us with detailedinformation of the genetic structure. The Bayesian approach was applied, detectingthree, as the most probable number of clusters. The similarity of eachsubpopulation using microsatellites was confirmed also by high-throughputmolecular data. The observed inbreeding (FROH=2.3%) was higher than thatexpected based on pedigree data (FPED=0.4%) due to the limited number ofavailable generations in pedigree data. One of the most important steps indevelopment of efficient autochthonous breed protection programs ischaracterization of genetic variability and assessment of the population structure.The chosen set of microsatellites confirmed the suitability in determination of thesubpopulations of Pinzgau cattle in Slovakia. The state of genetic diversity at moredetailed level was successfully performed using bovineSNP50 BeadChip.

Genetic diversity within Scorpio maurus (Scorpiones: Scorpionidae) from morocco: Preliminary evidence based on CO1 mitochondrial DNA sequences

Biologia ◽  
2008 ◽  
Vol 63 (6) ◽  
Author(s):  
Elsa Froufe ◽  
Pedro Sousa ◽  
Paulo Alves ◽  
David Harris
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Cytochrome Oxidase ◽  
Dna Sequences ◽  
Preliminary Analysis ◽  
Mitochondrial Gene ◽  
Sequence Divergence ◽  
Preliminary Evidence ◽  
Evidence Based ◽  
Cytochrome Oxidase 1

AbstractThe large-clawed scorpion, Scorpio maurus, is a medically important scorpion and yet nothing is known regarding genetic diversity within this species. As a preliminary analysis we determined variation within the cytochrome oxidase 1 (CO1) mitochondrial gene from specimens from Morocco. High levels of genetic diversity were found that presented some geographical coherence. Of the two identified subspecies from Morocco, S. maurus birulai and S. maurus fuliginosus, the latter included genetically distinct lineages (8.0% uncorrected sequence divergence), indicating a detailed morphological and molecular revision is needed for this species.

The mitochondrial DNA haplotypes of snails of the estuarine hydrobiid genus Tatea cross species and biogeographic boundaries

2009 ◽  
Vol 60 (8) ◽  
pp. 861 ◽  
Author(s):  
D. J. Colgan ◽  
P. da Costa
Keyword(s):  
Mitochondrial Dna ◽  
Dna Sequences ◽  
Marine Species ◽  
Habitat Diversity ◽  
12S Rrna ◽  
Genetic Structuring ◽  
Dna Variation ◽  
Eastern Australia ◽  
Mitochondrial 12S Rrna ◽  
Marine Littoral

Investigations of estuarine taxa can provide a perspective on phylogeography that complements studies of marine littoral organisms. For example, reductions in gene flow between populations and increased genetic structuring would be expected in estuarine species. The substantial amount of information about marine species and the habitat diversity along long latitudinal spans makes south-eastern Australia an excellent potential location for comparing marine and estuarine taxa. To investigate this potential, we studied the phylogeography of the two species in the estuarine gastropod genus Tatea. These have extensive and broadly overlapping distributions that encompass known marine phylogeographic boundaries. Against expectation, both Tatea species showed a remarkable lack of geographic and inter-specific variability in mitochondrial 12S rRNA (107 specimens) and cytochrome c oxidase subunit I (39) DNA sequences. No major phylogeographic discontinuities were revealed in either species and there was minimal haplotype divergence between them for either 12S rRNA or COI. The patterns of mitochondrial DNA variation discovered in Tatea may be due to a recent selective sweep or range expansion from a population in which there was little variability. Both possibilities are complicated by having to explain the similarity of the patterns in the two species.

Genetic Structure of the Western and Eastern African Sahel/Savannah Belt and the Role of Nomadic Pastoralists as Inferred from the Variation of D-Loop Mitochondrial DNA Sequences

Human Biology ◽  
2017 ◽  
Vol 89 (4) ◽  
pp. 281 ◽  
Author(s):  
Martina Čížková ◽  
Pavel Munclinger ◽  
Mame Yoro Diallo ◽  
Iva Kulichová ◽  
Mohammed G. Mokhtar ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Structure ◽  
Dna Sequences ◽  
Nomadic Pastoralists ◽  
D Loop ◽  
African Sahel
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