Introgression of B-genome chromosomes in a doubled haploid population of Brassica napus × B. carinata

Genome ◽  
2010 ◽  
Vol 53 (8) ◽  
pp. 619-629 ◽  
Author(s):  
Z. K. Navabi ◽  
I. A.P. Parkin ◽  
J. C. Pires ◽  
Z. Xiong ◽  
M. R. Thiagarajah ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Seed Quality ◽  
Agronomic Traits ◽  
Interspecific Cross ◽  
Leptosphaeria Maculans ◽  
Erucic Acid Content ◽  
Doubled Haploid Population ◽  
Dh Population ◽  
B Genome ◽  
Dh Lines

The Brassica B-genome species possess many valuable agronomic and disease resistance traits. To transfer traits from the B genome of B. carinata into B. napus , an interspecific cross between B. napus and B. carinata was performed and a doubled haploid (DH) population was generated from the BC2S3 generation. Successful production of interspecific DH lines as identified using B-genome microsatellite markers is reported. Five percent of DH lines carry either intact B-genome chromosomes or chromosomes that have deletions. All of the DH lines have linkage group J13/B7 in common. This was further confirmed using B. nigra genomic DNA in a fluorescent in situ hybridization assay where the B-genome chromosomes were visualized and distinguished from the A- and C-genome chromosomes. The 60 DH lines were also evaluated for morphological traits in the field for two seasons and were tested for resistance to blackleg, caused by Leptosphaeria maculans , under greenhouse conditions. Variation in the DH population followed a normal distribution for several agronomic traits and response to blackleg. The lines with B-genome chromosomes were significantly different (p < 0.01) from the lines without B-genome chromosomes for both morphological and seed quality traits such as days to flowering, days to maturity, and erucic acid content.

Mapping QTL controlling agronomic traits in a doubled haploid population of winter oilseed rape (Brassica napus L.)

2018 ◽  
Vol 97 (5) ◽  
pp. 1389-1406 ◽  
Author(s):  
Farshad Fattahi ◽  
Barat Ali Fakheri ◽  
Mahmood Solouki ◽  
Christian Möllers ◽  
Abbas Rezaizad
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Doubled Haploid ◽  
Agronomic Traits ◽  
Doubled Haploid Population ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
Mapping Qtl ◽  
Haploid Population

scholarly journals Development of Self-incompatible Lines with Improved Seed Quality in Winter Oilseed Rape (Brassica napus L.) for Hybrid Breeding

2011 ◽  
Vol 41 (No. 3) ◽  
pp. 105-111 ◽  
Author(s):  
R. Koprna ◽  
V. Kučera ◽  
O. Kolovrat ◽  
M. Vyvadilová ◽  
M. Klíma
Keyword(s):  
Erucic Acid ◽  
Doubled Haploid ◽  
Acid Content ◽  
Seed Quality ◽  
Quality Parameters ◽  
Hplc Method ◽  
Hybrid Breeding ◽  
Erucic Acid Content ◽  
Glucosinolate Content ◽  
Brassica Napus L

Doubled haploid (DH) self-incompatible (SI) regenerants with improved seed quality were derived after two improvement crossing cycles with different &ldquo;00&rdquo; quality donors. The original genetic resource of recessive sporophytic SI was characterised by a high glucosinolate (GSL) and erucic acid content. SI regenerants of satisfactory seed quality parameters were obtained from the second cycle of crossing. Total number of derived SI plants was 87, i.e. 38.2% out of 228 obtained fertile doubled haploid regenerants developed in 2004. Nine plants out of the analysed 45 SI DH regenerants had the erucic acid content lower than 2%. Five out of these nine plants, which were analysed by HPLC method, had the glucosinolate content lower than the limit 18 &micro;mol/g at 9% seed moisture. The method based on the spraying of inflorescences with 5% NaCl to enable reproduction of SI lines was verified. &nbsp;

scholarly journals Mapping of Novel QTL Regulating Grain Shattering Using Doubled Haploid Population in Rice (Oryza sativaL.)

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Gyu-Ho Lee ◽  
In-Kyu Kang ◽  
Kyung-Min Kim
Keyword(s):  
Doubled Haploid ◽  
Chromosome 1 ◽  
Doubled Haploid Population ◽  
Chromosome 6 ◽  
Abscission Layer ◽  
Dh Population ◽  
Severe Reduction ◽  
Rice Chromosomes ◽  
Evolutionary Step ◽  
Haploid Population

The critical evolutionary step during domestication of major cereals was elimination of seed shattering because the easy-to-shatter trait in wild relatives results in a severe reduction in yield. In this study, we analyzed the QTLs associated with shattering employing a high-density genetic map in doubled haploid (DH) population of rice (Oryza sativaL.). A genetic linkage map was generated with 217 microsatellite markers spanning 2082.4 cM and covering 12 rice chromosomes with an average interval of 9.6 cM between markers based on 120 DHLs derived from a cross between Cheongcheong indica type cultivar and Nagdong japonica type cultivar. In the QTL analysis, five QTLs pertaining to the breaking tensile strength (BTS) were detected in 2013 and 2015. Two regions of the QTLs related to BTS on chromosome 1 and chromosome 6 were detected. Several important genes are distributed in 1 Mbp region of the QTL on chromosome 6 and they are related to the formation of abscission layer. We decide to name this QTLqSh6and the candidate genes in theqSh6region can be employed usefully in further research for cloning.

scholarly journals Detection of drought tolerance-related QTL in the Plainsman V./Cappelle Desprez doubled haploid wheat population

2021 ◽  
Author(s):  
É. Nagy ◽  
Á. Szabó-Hevér ◽  
S. Lehoczki-Krsjak ◽  
C. Lantos ◽  
E. Kiss ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Grain Yield ◽  
Doubled Haploid ◽  
Agronomic Traits ◽  
Abiotic Factors ◽  
Phenotypic Variance ◽  
Thousand Grain Weight ◽  
Dh Population ◽  
Wheat Population ◽  
Irrigation Levels

AbstractDrought stress is one of the major abiotic factors that significantly reduces wheat grain yield. Improving drought tolerance is a challenge that plant breeders are facing nowadays. In this study, our goal was to identify quantitative trait loci (QTL) in the Plainsman V./Cappelle Desprez doubled haploid (DH) population under drought induced as decreased irrigation (ds) and well-watered (ww) conditions in glasshouse. In total, 54 QTL were detected across the three years in two water regimes linked to 10 drought tolerance-related agronomic traits. Out of the detected QTL regions several have been previously reported. The QTL on chromosome 1A (wPt-744613-wPt-8016) related to thousand grain weight was detected in both ds and ww conditions, explaining the 12.7–17.4% of the phenotypic variance. QTL for grain yield was detected on chromosomes 1A, and 6B in the ds treatment. Numerous QTL was identified under both irrigation levels.

scholarly journals Genetic Analysis of Agronomic Traits and Grain Iron and Zinc Concentrations in a Doubled Haploid Population of Rice (Oryza sativa L.)

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mark Ian C. Calayugan ◽  
Andrea Kariza Formantes ◽  
Amery Amparado ◽  
Gwen Iris Descalsota-Empleo ◽  
Chau Thanh Nha ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Genetic Analysis ◽  
Doubled Haploid ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Doubled Haploid Population ◽  
Iron And Zinc ◽  
Zinc Concentrations ◽  
Haploid Population

Mapping QTL for grain yield, yield components, and spike features in a doubled haploid population of bread wheat

Genome ◽  
2011 ◽  
Vol 54 (6) ◽  
pp. 517-527 ◽  
Author(s):  
Bahram Heidari ◽  
Badraldin Ebrahim Sayed-Tabatabaei ◽  
Ghodratollah Saeidi ◽  
Michael Kearsey ◽  
Kazuhiro Suenaga
Keyword(s):  
Grain Yield ◽  
Doubled Haploid ◽  
Yield Components ◽  
Triticum Aestivum L ◽  
Interval Mapping ◽  
Doubled Haploid Population ◽  
Dh Population ◽  
Spike Number ◽  
Haploid Population ◽  
Major Qtl

A doubled haploid (DH) population derived from a cross between the Japanese cultivar ‘Fukuho-kumogi’ and the Israeli wheat line ‘Oligoculm’ was used to map genome regions involved in the expression of grain yield, yield components, and spike features in wheat (Triticum aestivum L). A total of 371 markers (RAPD, SSR, RFLP, AFLP, and two morphological traits) were used to construct the linkage map that covered 4190 cM of wheat genome including 28 linkage groups. The results of composite interval mapping for all studied traits showed that some of the quantitative trait loci (QTL) were stable over experiments conducted in 2004 and 2005. The major QTL located in the Hair–Xpsp2999 interval on chromosome 1A controlled the expression of grains/spike (R2 = 12.9% in 2004 and 22.4% in 2005), grain weight/spike (R2 = 21.4% in 2004 and 15.8% in 2005), and spike number (R2 = 15.6% in 2004 and 5.4% in 2005). The QTL for grain yield located on chromosomes 6A, 6B, and 6D totally accounted for 27.2% and 31.7% of total variation in this trait in 2004 and 2005, respectively. Alleles inherited from ‘Oligoculm’ increased the length of spikes and had decreasing effects on spike number. According to the data obtained in 2005, locus Xgwm261 was associated with a highly significant spike length QTL (R2 = 42.33%) and also the major QTL for spikelet compactness (R2 = 26.1%).

scholarly journals Inheritance and variation of erucic acid content in a transgenic rapeseed (Brassica napus L.) doubled haploid population

2008 ◽  
Vol 23 (1) ◽  
pp. 125-138 ◽  
Author(s):  
Ujjal K. Nath ◽  
Gayatri Goswami ◽  
Rosemarie Clemens ◽  
Heiko C. Becker ◽  
Christian Möllers
Keyword(s):  
Brassica Napus ◽  
Erucic Acid ◽  
Doubled Haploid ◽  
Acid Content ◽  
Erucic Acid Content ◽  
Doubled Haploid Population ◽  
Brassica Napus L ◽  
Haploid Population ◽  
Transgenic Rapeseed

scholarly journals Inheritance of seed quality and seed germination in two doubled haploid populations of oilseed rape segregating for acid detergent lignin (ADL) content

Euphytica ◽  
2021 ◽  
Vol 217 (8) ◽  
Author(s):  
Sasanti Widiarsih ◽  
Manuela Nagel ◽  
Andreas Börner ◽  
Kirstin Feussner ◽  
Ivo Feussner ◽  
...  
Keyword(s):  
Seed Germination ◽  
Oilseed Rape ◽  
Doubled Haploid ◽  
Field Experiments ◽  
Seed Quality ◽  
Lignin Content ◽  
Gene Copy ◽  
Seed Color ◽  
Metabolome Analysis ◽  
Dh Lines

AbstractThe high lignin content of the seed hull of oilseed rape leads to its black seed color compared to yellow seeded soybean. Reducing lignin and increasing oil and protein content is an important breeding aim in oilseed rape. The objective of the present study was to analyze the inheritance of two rapeseed genetic resources with reduced lignin content in the seeds and to elucidate the effects on seed germination and composition. These resources were crossed to black seeded Express 617 and F1 plants were used to develop two doubled haploid (DH) populations. The two DH populations were tested in field experiments in three to five environments. Significant genetic variation for lignin content and bimodal frequency distributions were found in both populations. SNP marker segregation in contrasting bulks revealed for both populations overlapping narrow genomic regions on chromosome C03, responsible for reduced lignin content. The low lignin bulks had a significantly higher percentage of seeds showing bacterial and fungal growth, but germination and vigor was not affected. Non-targeted metabolome analysis of immature green seeds showed exclusively a depletion of metabolites of the proanthocyanidin pathway in DH lines with a reduced lignin content. In these DH lines, a cinnamate-4-hydroxylase gene copy was identified as candidate gene in the overlapping genomic region of both populations. The newly identified QTL for reduced lignin content on chromosome C03 is valuable for studying additive and epistatic effects in combination with other low lignin genotypes.

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