Mapping QTL for grain yield, yield components, and spike features in a doubled haploid population of bread wheat

Genome ◽  
2011 ◽  
Vol 54 (6) ◽  
pp. 517-527 ◽  
Author(s):  
Bahram Heidari ◽  
Badraldin Ebrahim Sayed-Tabatabaei ◽  
Ghodratollah Saeidi ◽  
Michael Kearsey ◽  
Kazuhiro Suenaga
Keyword(s):  
Grain Yield ◽  
Doubled Haploid ◽  
Yield Components ◽  
Triticum Aestivum L ◽  
Interval Mapping ◽  
Doubled Haploid Population ◽  
Dh Population ◽  
Spike Number ◽  
Haploid Population ◽  
Major Qtl

A doubled haploid (DH) population derived from a cross between the Japanese cultivar ‘Fukuho-kumogi’ and the Israeli wheat line ‘Oligoculm’ was used to map genome regions involved in the expression of grain yield, yield components, and spike features in wheat (Triticum aestivum L). A total of 371 markers (RAPD, SSR, RFLP, AFLP, and two morphological traits) were used to construct the linkage map that covered 4190 cM of wheat genome including 28 linkage groups. The results of composite interval mapping for all studied traits showed that some of the quantitative trait loci (QTL) were stable over experiments conducted in 2004 and 2005. The major QTL located in the Hair–Xpsp2999 interval on chromosome 1A controlled the expression of grains/spike (R2 = 12.9% in 2004 and 22.4% in 2005), grain weight/spike (R2 = 21.4% in 2004 and 15.8% in 2005), and spike number (R2 = 15.6% in 2004 and 5.4% in 2005). The QTL for grain yield located on chromosomes 6A, 6B, and 6D totally accounted for 27.2% and 31.7% of total variation in this trait in 2004 and 2005, respectively. Alleles inherited from ‘Oligoculm’ increased the length of spikes and had decreasing effects on spike number. According to the data obtained in 2005, locus Xgwm261 was associated with a highly significant spike length QTL (R2 = 42.33%) and also the major QTL for spikelet compactness (R2 = 26.1%).

scholarly journals Mapping of Novel QTL Regulating Grain Shattering Using Doubled Haploid Population in Rice (Oryza sativaL.)

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Gyu-Ho Lee ◽  
In-Kyu Kang ◽  
Kyung-Min Kim
Keyword(s):  
Doubled Haploid ◽  
Chromosome 1 ◽  
Doubled Haploid Population ◽  
Chromosome 6 ◽  
Abscission Layer ◽  
Dh Population ◽  
Severe Reduction ◽  
Rice Chromosomes ◽  
Evolutionary Step ◽  
Haploid Population

The critical evolutionary step during domestication of major cereals was elimination of seed shattering because the easy-to-shatter trait in wild relatives results in a severe reduction in yield. In this study, we analyzed the QTLs associated with shattering employing a high-density genetic map in doubled haploid (DH) population of rice (Oryza sativaL.). A genetic linkage map was generated with 217 microsatellite markers spanning 2082.4 cM and covering 12 rice chromosomes with an average interval of 9.6 cM between markers based on 120 DHLs derived from a cross between Cheongcheong indica type cultivar and Nagdong japonica type cultivar. In the QTL analysis, five QTLs pertaining to the breaking tensile strength (BTS) were detected in 2013 and 2015. Two regions of the QTLs related to BTS on chromosome 1 and chromosome 6 were detected. Several important genes are distributed in 1 Mbp region of the QTL on chromosome 6 and they are related to the formation of abscission layer. We decide to name this QTLqSh6and the candidate genes in theqSh6region can be employed usefully in further research for cloning.

scholarly journals RESPONS OF BREAD WHEAT YIELD AND IT,S COMPONENTS TO BORON AND VITAMIN C FOLIAR APPLIED

2016 ◽  
Vol 47 (5) ◽  
Author(s):  
Al-Hilfy & Zeboon
Keyword(s):  
Vitamin C ◽  
Grain Yield ◽  
Bread Wheat ◽  
Yield Components ◽  
Wheat Yield ◽  
Triticum Aestivum L ◽  
College Of Agriculture ◽  
Field Crops ◽  
Two Factors ◽  
Spike Number

Field experiment was conducted during 2010 – 2011 and 2011 – 2012 winter seasons at the Experimental Farm, Department of Field Crops, College of Agriculture University of Baghdad. RCBD design with four replications were used to study the effect of Boron and vitamin C foliar applied on yield and some it,s components of bread  wheat Triticum aestivum L. cv. Abu – Ghraib-3. The experiment involved two factors ,the first was boron foliar in five concentrations  0,100, 200, 300, 400 mg L-1  the second was vitamin C foliar applied in five concentrations 0,1,2,3,4, gmL-1 at booting stage. Results of the experiment showed :300 mg B L-1 was  superior in grain yield 6.726,6.771 Mg ha-1)   for both seasons and some yield components ( spike number for the second season 574.9 spike m-2 , fertility 97.27% 97.34% and number of grain 66.39 , 65,22 grain spik-1 ) for both seasons . Foliar of Vitamin C with 4 gm L-1 was significantly affected grain yield (6.820 , 6.879 Mg ha-1 ) for both seasons respectively . Foliar of Vitamin C with 4 gm L-1 was significantly affected on grain yield and some it,s components (6.820 , 6.878 Mg ha-1 ) as compared to control which gave (4.882 ,4.906 Mg ha-1) for both seasons, respectively .The interaction between two factors was significant in some characters ( number of spike and grain yield ).

Quantitative trait locus analysis of late maturity a-amylase in wheat using the doubled haploid population Cranbrook Halberd

2001 ◽  
Vol 52 (12) ◽  
pp. 1267 ◽  
Author(s):  
K. Mrva ◽  
D. J. Mares
Keyword(s):  
Quantitative Trait Locus ◽  
Doubled Haploid ◽  
Quantitative Trait ◽  
Triticum Aestivum L ◽  
Temperature Treatment ◽  
Doubled Haploid Population ◽  
Cool Temperature ◽  
Haploid Population ◽  
Late Maturity ◽  
Trait Locus

Mapping of the late maturity α-amylase (LMA) gene using quantitative trait locus (QTL) analysis represents an important step in identification of potential molecular markers that would greatly improve efficiency and accuracy of screening for LMA. QTL controlling the expression of LMA in wheat were detected in a doubled haploid (DH) cross/population derived from wheat (Triticum aestivum L. em. Thell) cultivars Cranbrook (LMA source) and Halberd (non-LMA). The DH population and parents were sown in replicated trials at Narrabri with sowing times differing by 2 weeks. Cool temperature treatment of detached tillers was used to induce expression of LMA in lines carrying the defect. The number of grains in ripe, treated tillers that contained high pI (malt, germination type) α-amylase isozymes was measured using an ELISA antibody kit highly specific for high pI isozymes. QTL analyses were conducted separately for each sowing, but results from both sowings were consistent and indicated that there was a highly significant (P < 0.001) QTL on the long arm of chromosome 7B (accounting for 31% of the variation in the first experiment), with Cranbrook contributing the higher value allele. A second QTL that accounted for 13% of the variation was found close to the centromere on chromosome 3B. Although it was less important than the QTL on 7B it was nevertheless still significant (P < 0.05).

Gametophytic selection in a winter × spring barley cross

Genome ◽  
1991 ◽  
Vol 34 (6) ◽  
pp. 918-922 ◽  
Author(s):  
C. C. Schön ◽  
P. M. Hayes ◽  
T. K. Blake ◽  
S. J. Knapp
Keyword(s):  
Segregation Distortion ◽  
Doubled Haploid ◽  
Doubled Haploids ◽  
Spring Barley ◽  
Population Based ◽  
Doubled Haploid Population ◽  
Dh Population ◽  
Gametophytic Selection ◽  
Segregation Ratios ◽  
Haploid Population

Segregation distortion and the consequences of gametophytic selection were assessed in a winter × spring barley cross by comparing segregation of enzyme, storage protein, DNA, and morphological markers in three populations derived from the same cross: a control F2 (F2C), a doubled-haploid (DH) population, and an F2 derived from F1 plants self-pollinated at 10 °C (F2T). Segregation distortion was present in the F2T and the doubled-haploid population. Based on a comparison of the F2C and the F2T, gametophytic selection as a consequence of self-pollination at 10 °C was operative on chromosome 7 in regions linked to Rrn2. Segregation distortion in favor of the winter parent was found in the doubled-haploid population. There were significant deviations from expected segregation ratios at two loci, but only at one of the loci was the gene number significantly different from the F2C. Despite segregation distortion, the doubled-haploid population should be suitable for linkage analyses, as estimates of recombination based on F2 and doubled-haploid data were in close agreement.Key words: Hordeum vulgare, segregation distortion, doubled haploids, gametophytic selection, cold tolerance.

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