Chromosomal location of the cadmium uptake gene (Cdu1) in durum wheat

Genome ◽  
2009 ◽  
Vol 52 (9) ◽  
pp. 741-747 ◽  
Author(s):  
R. E. Knox ◽  
C. J. Pozniak ◽  
F. R. Clarke ◽  
J. M. Clarke ◽  
S. Houshmand ◽  
...  
Keyword(s):  
Triticum Turgidum ◽  
Safety Concern ◽  
Dominant Gene ◽  
Doubled Haploid Population ◽  
Cd Uptake ◽  
Laboratory Methods ◽  
Breeding Programs ◽  
Chromosome 5B ◽  
Haploid Population ◽  
Trait Locus

Levels of the heavy metal cadmium (Cd) in food products are a food safety concern. Grain Cd is higher in durum (Triticum turgidum L. var. durum) than in common wheat, so reduction of Cd in durum grain is a priority of breeding programs. Previous research demonstrated that a single dominant gene, Cdu1, confers the low grain Cd phenotype, but the map location of the gene is not known. A doubled haploid population segregating for Cd concentration, developed from the cross of W9262-260D3 (a Kyle*2/Biodur inbred selection with low Cd uptake) and Kofa (high Cd uptake) and mapped with microsatellite markers, was used to locate Cdu1. Grain Cd concentration was determined by standard laboratory methods on field grain samples in 2000 and 2001. The Cd concentration segregated bimodally, allowing Cdu1 to be mapped qualitatively as well as quantitatively with quantitative trait locus analysis. The Cdu1 gene mapped to the long arm of chromosome 5B.

scholarly journals Mapping and QTL analysis of the barley population Chebec × Harrington

2003 ◽  
Vol 54 (12) ◽  
pp. 1125 ◽  
Author(s):  
A. R. Barr ◽  
A. Karakousis ◽  
R. C. M. Lance ◽  
S. J. Logue ◽  
S. Manning ◽  
...  
Keyword(s):  
Cyst Nematode ◽  
Cereal Cyst Nematode ◽  
Malting Quality ◽  
Doubled Haploid Population ◽  
Malt Extract ◽  
Malting Barley ◽  
Breeding Programs ◽  
Genomic Location ◽  
Haploid Population ◽  
Barley Population

A doubled haploid population of 120 individuals was produced from the parents Chebec, an Australian 2-row barley of feed quality with resistance to the cereal cyst nematode, and Harrington, a 2-rowed, Canadian variety of premium malting quality. This paper describes 18 field and laboratory experiments conducted with the population and summarises the traits mapped and analysed. The genomic location of 25 traits and genes is described and marker–trait associations for 5 traits (malt extract, diastatic power, resistance to cereal cyst nematode, early flowering, resistance to pre-harvest sprouting) important to Australian efforts to improve malting barley varieties have been used in practical breeding programs. Detailed maps for these populations are shown in this paper, while a consensus map incorporating these maps and further experiments on the populations are described elsewhere in this issue.

scholarly journals Mapping Quantitative Trait Loci for 1000-Grain Weight in a Double Haploid Population of Common Wheat

2020 ◽  
Vol 21 (11) ◽  
pp. 3960 ◽  
Author(s):  
Tao Liu ◽  
Lijun Wu ◽  
Xiaolong Gan ◽  
Wenjie Chen ◽  
Baolong Liu ◽  
...  
Keyword(s):  
Quantitative Trait ◽  
Genetic Distances ◽  
Snp Markers ◽  
Grain Weight ◽  
High Yield ◽  
Doubled Haploid Population ◽  
Phenotypic Variance ◽  
Yield Trait ◽  
Haploid Population ◽  
Trait Locus

Thousand-grain weight (TGW) is a very important yield trait of crops. In the present study, we performed quantitative trait locus (QTL) analysis of TGW in a doubled haploid population obtained from a cross between the bread wheat cultivar “Superb” and the breeding line “M321” using the wheat 55-k single-nucleotide polymorphism (SNP) genotyping assay. A genetic map containing 15,001 SNP markers spanning 2209.64 cM was constructed, and 9 QTLs were mapped to chromosomes 1A, 2D, 4B, 4D, 5A, 5D, 6A, and 6D based on analyses conducted in six experimental environments during 2015–2017. The effects of the QTLs qTgw.nwipb-4DS and qTgw.nwipb-6AL were shown to be strong and stable in different environments, explaining 15.31–32.43% and 21.34–29.46% of the observed phenotypic variance, and they were mapped within genetic distances of 2.609 cM and 5.256 cM, respectively. These novel QTLs may be used in marker-assisted selection in wheat high-yield breeding.

Mapping and QTL analysis of the barley population Clipper × Sahara

2003 ◽  
Vol 54 (12) ◽  
pp. 1137 ◽  
Author(s):  
A. Karakousis ◽  
A. R. Barr ◽  
J. M. Kretschmer ◽  
S. Manning ◽  
S. P. Jefferies ◽  
...  
Keyword(s):  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Nematode Resistance ◽  
Cyst Nematode ◽  
Doubled Haploid Population ◽  
Plant Type ◽  
Boron Tolerance ◽  
Breeding Programs ◽  
Haploid Population ◽  
Barley Population

A genetic linkage map consisting of 211 molecular markers has been generated using a doubled-haploid population derived from a cross between the Australian barley variety Clipper and the Algerian landrace Sahara 3771. The map was used in subsequent trait mapping studies to locate the genes conferring boron tolerance and cereal cyst nematode resistance from Sahara 3371 and to map several plant type and developmental genes. Closely linked markers to the trait loci have been identified and are now being widely implemented in Australian breeding programs.

Quantitative trait locus analysis of late maturity a-amylase in wheat using the doubled haploid population Cranbrook Halberd

2001 ◽  
Vol 52 (12) ◽  
pp. 1267 ◽  
Author(s):  
K. Mrva ◽  
D. J. Mares
Keyword(s):  
Quantitative Trait Locus ◽  
Doubled Haploid ◽  
Quantitative Trait ◽  
Triticum Aestivum L ◽  
Temperature Treatment ◽  
Doubled Haploid Population ◽  
Cool Temperature ◽  
Haploid Population ◽  
Late Maturity ◽  
Trait Locus

Mapping of the late maturity α-amylase (LMA) gene using quantitative trait locus (QTL) analysis represents an important step in identification of potential molecular markers that would greatly improve efficiency and accuracy of screening for LMA. QTL controlling the expression of LMA in wheat were detected in a doubled haploid (DH) cross/population derived from wheat (Triticum aestivum L. em. Thell) cultivars Cranbrook (LMA source) and Halberd (non-LMA). The DH population and parents were sown in replicated trials at Narrabri with sowing times differing by 2 weeks. Cool temperature treatment of detached tillers was used to induce expression of LMA in lines carrying the defect. The number of grains in ripe, treated tillers that contained high pI (malt, germination type) α-amylase isozymes was measured using an ELISA antibody kit highly specific for high pI isozymes. QTL analyses were conducted separately for each sowing, but results from both sowings were consistent and indicated that there was a highly significant (P < 0.001) QTL on the long arm of chromosome 7B (accounting for 31% of the variation in the first experiment), with Cranbrook contributing the higher value allele. A second QTL that accounted for 13% of the variation was found close to the centromere on chromosome 3B. Although it was less important than the QTL on 7B it was nevertheless still significant (P < 0.05).

scholarly journals Novel Septoria Speckled Leaf Blotch Resistance Loci in a Barley Doubled-Haploid Population

2012 ◽  
Vol 102 (7) ◽  
pp. 683-691 ◽  
Author(s):  
Doris Luckert ◽  
Hala Toubia-Rahme ◽  
Brian J. Steffenson ◽  
Thin-Meiw Choo ◽  
Stephen J. Molnar
Keyword(s):  
Dna Sequences ◽  
Doubled Haploid ◽  
Gene Clusters ◽  
Doubled Haploid Population ◽  
Resistance Locus ◽  
Marker Assisted Breeding ◽  
Leaf Blotch ◽  
Diversity Arrays Technology ◽  
Haploid Population ◽  
Trait Locus

The genetics of resistance to Septoria speckled leaf blotch (SSLB), caused by Septoria passerinii, was studied in the Leger × CIho9831 barley doubled-haploid population. The 140 lines in the population segregated as 102 resistant and 38 susceptible, approximating a 3:1 ratio. A recombination map was developed using diversity arrays technology and other molecular markers. Quantitative trait locus (QTL) analysis demonstrated that resistance is primarily conferred either by having the CIho9831 allele at a QTL on 6HS or by having the CIho9831 allele at both of two QTLs on 3H and 2HL. In addition, ≈1/16 of the lines were resistant for unidentified reasons. This model predicts a resistant/susceptible ratio of 11:5, which fits the phenotypic observations. Minor QTLs were detected on 2HS and 1H. DNA sequences of linked markers suggest that the 6HS, 3H, and 2HS QTLs are part of resistance gene clusters and that the 6HS and 3H QTLs share homology. The 6HS QTL is identical to or closely linked to the SSLB resistance locus Rsp4 and the 1H QTL to the Rsp2 or Rsp3 locus. The 3H and 2HS QTLs are unique and offer new opportunities for pyramiding resistance genes through marker-assisted breeding for resistance to S. passerinii.

A quantitative trait locus on chromosome 5B controls resistance of Triticum turgidum (L.) var. diccocoides to Stagonospora nodorum blotch

Euphytica ◽  
2008 ◽  
Vol 166 (2) ◽  
pp. 199-206 ◽  
Author(s):  
J. L. Gonzalez-Hernandez ◽  
P. K. Singh ◽  
M. Mergoum ◽  
T. B. Adhikari ◽  
S. F. Kianian ◽  
...  
Keyword(s):  
Quantitative Trait Locus ◽  
Quantitative Trait ◽  
Triticum Turgidum ◽  
Stagonospora Nodorum ◽  
Stagonospora Nodorum Blotch ◽  
Chromosome 5B ◽  
Trait Locus

QTLs and their interaction determining different heading dates of barley in Australia and China

2010 ◽  
Vol 61 (2) ◽  
pp. 145 ◽  
Author(s):  
Xifeng Ren ◽  
Chengdao Li ◽  
W. J. R. Boyd ◽  
Sharon Westcott ◽  
C. R. Grime ◽  
...  
Keyword(s):  
Phenotypic Variation ◽  
Chromosomal Location ◽  
Heading Date ◽  
Doubled Haploid Population ◽  
Seasonal Adaptation ◽  
Breeding Programs ◽  
Haploid Population ◽  
Barley Germplasm ◽  
Late Genotype ◽  
Late Sowing

Heading date is a major determinant of the regional and seasonal adaptation of barley varieties. The dogma is that introduced germplasm is more likely to be adapted if it is derived from a similar latitude. However, barley germplasm introduced from similar latitudes of South-East Asia is extremely early heading in the Australian environments and vice versa. A doubled-haploid population from a cross of an Australian barley Galleon and a Japanese barley Haruna Nijo was evaluated for heading date in Australia (Perth, 31°56′S) and China (Wuhan, 30°33′N) under normal autumn sowing, late sowing in the field, and extended-light glasshouse conditions. One major QTL was identified on chromosome 5H under the three conditions in China. The single QTL accounted for up to 50% of phenotypic variation for heading date. The Australian variety contributed to late heading date. Two QTLs on chromosomes 4H and 5H were detected for controlling heading date in Australia. The QTL/QTL interaction contributed up to 35.8% of phenotypic variation for heading date in Australia, which is the major reason for the extremely early heading date of the Japanese variety in the Australian environment. The chromosome 5H QTL was detected at the same chromosomal location when the population was grown in either China or Australia. In both environments the Australian variety contributed to the late heading date. Selection against the Japanese alleles of chromosomes 4H and 5H QTLs could eliminate the extremely early genotype in Australia and selection against the Australian allele of chromosome 5H QTL could eliminate the extremely late genotype in China when Australian and Japanese germplasms are used in the breeding programs.

Direct PCR-based genetic mapping of rice telomeric repeat associated sequences

Genome ◽  
1998 ◽  
Vol 41 (2) ◽  
pp. 193-198 ◽  
Author(s):  
Lishuang Shen ◽  
Lihuang Zhu
Keyword(s):  
Genetic Mapping ◽  
Oryza Sativa L ◽  
Telomeric Repeat ◽  
Doubled Haploid Population ◽  
Direct Pcr ◽  
Japonica Variety ◽  
Rapd Primer ◽  
Associated Sequences ◽  
Haploid Population ◽  
Pcr Method

Direct PCR-based genetic mapping of telomeric repeat associated sequences (TASs) was achieved using a RAPD primer mediated asymmetric PCR method. Twenty-two TAS loci were mapped in a rice doubled haploid population derived from a cross between an indica variety (Zhaiyeqing8) and a japonica variety (Jingxi17). Of these, 11 loci were mapped to the most distal position of seven chromosome arms and lengthened the linkage groups by 7.4-22.6 cM, five were mapped to the approximate positions of the centromeric regions, and six were mapped to other interstitial chromosomal regions.Key words: rice, Oryza sativa L., genetic mapping, telomeric repeat, telomeric repeat associated sequences, RAPD primer mediated PCR.

Mapping QTL controlling agronomic traits in a doubled haploid population of winter oilseed rape (Brassica napus L.)

2018 ◽  
Vol 97 (5) ◽  
pp. 1389-1406 ◽  
Author(s):  
Farshad Fattahi ◽  
Barat Ali Fakheri ◽  
Mahmood Solouki ◽  
Christian Möllers ◽  
Abbas Rezaizad
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Doubled Haploid ◽  
Agronomic Traits ◽  
Doubled Haploid Population ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
Mapping Qtl ◽  
Haploid Population
Sign in / Sign up

Export Citation Format

Share Document