Integration of microsatellite-based genetic maps for the turkey (Meleagris gallopavo)

Genome ◽  
2006 ◽  
Vol 49 (10) ◽  
pp. 1308-1318 ◽  
Author(s):  
K.M. Reed ◽  
L.D. Chaves ◽  
T.P. Knutson ◽  
S.B. Krueth ◽  
C.M. Ashwell ◽  
...  
Keyword(s):  
Chicken Genome ◽  
Meleagris Gallopavo ◽  
Genetic Maps ◽  
University Of Minnesota ◽  
Genetic Studies ◽  
Draft Assembly ◽  
Chicken Genome Sequence ◽  
Genetic Linkages ◽  
Breeding Farms ◽  
The University

Integration of turkey genetic maps and their associated markers is essential to increase marker density in support of map-based genetic studies. The objectives of this study were to integrate 2 microsatellite-based turkey genetic maps — the Roslin map and the University of Minnesota (UMN) map — by genotyping markers from the Roslin study on the mapping families of the UMN study. A total of 279 markers was tested, and 240 were subsequently screened for polymorphisms in the UMN/Nicholas Turkey Breeding Farms (NTBF) mapping families. Of the 240 markers, 89 were genetically informative and were used for genotyping the F2 offspring. Significant genetic linkages (log of odds > 3.0) were found for 84 markers from the Roslin study. BLASTn comparison of marker sequences with the draft assembly of the chicken genome found 263 significant matches. The combination of genetic and in silico mapping allowed for the alignment of all linkage groups of the Roslin map with those of the UMN map. With the addition of the markers from the Roslin map, 438 markers are now genetically linked in the UMN/NTBF families, and more than 1700 turkey sequences have now been assigned to likely positions in the chicken-genome sequence.

One hundred fifty-four genetic markers for the turkey (Meleagris gallopavo)

Genome ◽  
2004 ◽  
Vol 47 (6) ◽  
pp. 1015-1028 ◽  
Author(s):  
Todd P Knutson ◽  
Lee D Chaves ◽  
Majken K Hall ◽  
Kent M Reed
Keyword(s):  
Genetic Linkage ◽  
Tandem Repeats ◽  
Genetic Research ◽  
Meleagris Gallopavo ◽  
Reference Population ◽  
University Of Minnesota ◽  
Important Species ◽  
Genomic Libraries ◽  
Breeding Farms ◽  
The University

Identifying and selectively breeding for improved traits is one of the ultimate goals of genetic research in agriculturally important species. Genome characterization and analysis are important first steps in this process. Genetic linkage maps based on the linear order of polymorphic DNA markers are typically developed through statistical analysis of inheritance patterns in pedigreed families. To develop microsatellite markers for further improvement of the turkey genetic linkage map, small-insert genomic libraries were screened for tandem repeats. Oligonuclotide primers were designed to amplify 164 microsatellite-containing fragments from genomic DNA. Genetic polymorphisms at 154 markers were determined by genotyping the F1 individuals of two resource populations. Markers determined as segregating in the University of Minnesota/Nicholas Turkey Breeding Farms (UMN/NTBF) reference population were used to genotype F2 individuals and a two-point linkage analysis was performed.Key words: turkey, Meleagris gallopavo, microsatellites, genetic linkage.

scholarly journals Isolation of 11 new polymorphic microsatellites from CA enriched turkey genomic libraries (Short communication)

2010 ◽  
Vol 53 (5) ◽  
pp. 618-622
Author(s):  
E. Korom ◽  
K. Bakos ◽  
G. Veress ◽  
O. Pinke ◽  
K. M. Reed ◽  
...  
Keyword(s):  
Short Communication ◽  
Mapping Population ◽  
Meleagris Gallopavo ◽  
University Of Minnesota ◽  
Microsatellite Repeat ◽  
Genomic Databases ◽  
Ca Repeat ◽  
Genomic Libraries ◽  
Polymorphic Microsatellites ◽  
The University

Abstract. Microsatellite loci from the ancient Hungarian variety of the Broad Breasted Bronze Turkey (Meleagris gallopavo) were isolated. CA-repeat enriched libraries were constructed from DNA of randomly collected samples. Libraries were screened for repeat-containing clones by PIMA (PCR Isolation of Microsatellite Arrays) and the DNA-sequence of 167 positive clones was determined. A total of 136 microsatellite repeat-containing sequences were found, 59 sequences were unique. Comparing these with the genomic databases, we found 7 previously annotated microsatellite sequences. The newly isolated 52 microsatellites were tested on the mapping population of the University of Minnesota, and the map position of 11 microsatellites was determined.

The Effects of Presentation on Noise and Dental Appliances on Speech

1981 ◽  
Vol 24 (1) ◽  
pp. 151-151
Author(s):  
Lillian Glass ◽  
Sharon R. Garber ◽  
T. Michael Speidel ◽  
Gerald M. Siegel ◽  
Edward Miller
Keyword(s):  
Southern California ◽  
University Of Southern California ◽  
University Of Minnesota ◽  
School Of Medicine ◽  
Southern California School ◽  
School Of Dentistry ◽  
The University

An omission in the Table of Contents, December JSHR, has occurred. Lillian Glass, Ph.D., at the University of Southern California School of Medicine and School of Dentistry, was a co-author of the article "The Effects of Presentation on Noise and Dental Appliances on Speech" along with Sharon R. Garber, T. Michael Speidel, Gerald M. Siegel, and Edward Miller of the University of Minnesota, Minneapolis.

Integrating Legacy Laboratory Information Systems into a Client-Server World: The University of Minnesota Clinical Workstation (CWS) Project

1995 ◽  
Vol 34 (03) ◽  
pp. 289-296 ◽  
Author(s):  
B. H. Sielaff ◽  
D. P. Connelly ◽  
K. E. Willard
Keyword(s):  
Information Systems ◽  
Clinical Decision ◽  
Database Management System ◽  
University Of Minnesota ◽  
Client Server ◽  
Clinical Workstation ◽  
Future Hospital ◽  
The Cost ◽  
The University ◽  
Laboratory Information Systems

Abstract:The development of an innovative clinical decision-support project such as the University of Minnesota’s Clinical Workstation initiative mandates the use of modern client-server network architectures. Preexisting conventional laboratory information systems (LIS) cannot be quickly replaced with client-server equivalents because of the cost and relative unavailability of such systems. Thus, embedding strategies that effectively integrate legacy information systems are needed. Our strategy led to the adoption of a multi-layered connection architecture that provides a data feed from our existing LIS to a new network-based relational database management system. By careful design, we maximize the use of open standards in our layered connection structure to provide data, requisition, or event messaging in several formats. Each layer is optimized to provide needed services to existing hospital clients and is well positioned to support future hospital network clients.

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