Linkage map of Cucumis melo including phenotypic traits and sequence-characterized genes

Genome ◽  
2003 ◽  
Vol 46 (5) ◽  
pp. 761-773 ◽  
Author(s):  
Leah Silberstein ◽  
Irina Kovalski ◽  
Yariv Brotman ◽  
Christophe Perin ◽  
Catherine Dogimont ◽  
...  
Keyword(s):  
Linkage Map ◽  
Resistance Gene ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Simple Sequence Repeats ◽  
Cucumis Melo ◽  
Fragment Length Polymorphism ◽  
Rflp Markers ◽  
Phenotypic Traits ◽  
Simple Sequence

A new linkage map of Cucumis melo, derived from the F2 progeny of a cross between PI 414723 and C. melo 'TopMark' is presented. The map spans a total of 1421 cM and includes 179 points consisting of random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), inter-simple sequence repeats (ISSRs), simple sequence repeats (SSRs), and restriction fragment length polymorphism (RFLP) markers. The map also includes an aphid resistance trait (Vat) and the sex type gene, andromonoecious (a), the two of which are important in resistance breeding and the control of hybrid seed production, as well as a seed-color gene, Wt-2. Most RFLPs represent sequence-characterized cDNA probes from C. melo and Cucumis sativus. These include resistance gene homologues and genes involved in various aspects of plant development and metabolism. A sub-set of our SSR and RFLP markers were also mapped, as part of this study, on additional mapping populations that were published for this species. This provides important reference points ("anchors"), enabling us to identify several linkage groups with respect to other melon maps.Key words: Cucumis melo, melon, genetic map, molecular markers, resistance gene homologues.

scholarly journals A Genetic Linkage Map of Olive Based on Amplified Fragment Length Polymorphism, Intersimple Sequence Repeat and Simple Sequence Repeat Markers

2010 ◽  
Vol 135 (6) ◽  
pp. 548-555 ◽  
Author(s):  
Bouchaib Khadari ◽  
Amal Zine El Aabidine ◽  
Cinderella Grout ◽  
Inès Ben Sadok ◽  
Agnès Doligez ◽  
...  
Keyword(s):  
Linkage Map ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Simple Sequence Repeat ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Issr Markers ◽  
Phenotypic Characterization ◽  
Sequence Repeat ◽  
Simple Sequence

A detailed genomic linkage map of the olive [Olea europaea L. ssp. europaea (2x = 2n = 46)] was constructed with a 147 F1 full-sib ‘Olivière’ × ‘Arbequina’ progeny in a two-way pseudo-test cross-mapping configuration. Based on a logarithm of odds threshold of 6 and a maximum recombination fraction of 0.4, maternal and paternal maps were constructed using 222 makers [178 amplified fragment length polymorphism (AFLP), 37 simple sequence repeat (SSR), seven intersimple sequence repeat (ISSR)] and 219 markers (174 AFLP, 39 SSR, 6 ISSR) markers, respectively. The female map regrouped 36 linkage groups (LGs) defining 2210.2 cM of total map length with an average marker spacing 11.2 cM and a maximum gap of 48.5 cM between adjacent markers. The male map contained 31 LGs and covered a distance of 1966.2 cM with an average and a maximum distance between two adjacent markers of 10.3 and 40.4 cM, respectively. Mean LG size was 61.3 and 63.4 cM in the maternal and paternal maps, respectively. The LGs consisted of two to 17 loci (up to 21 loci in the paternal map) and ranged in length from 2.7 to 182 cM (female map) or from 4.1 to 218.1 cM (paternal map). Markers were distributed throughout the maps without any clustering. The total length of the consensus map was 3823.2 cM containing 436 markers distributed into 42 LGs with a mean distance between two adjacent loci of 8.7 cM. Both parental maps and the consensus maps were compared with previously published olive maps. Although not saturated yet, the present maps offer a promising tool for quantitative trait loci mapping because phenotypic characterization of the cross is currently carried out.

Assessment of genome origins and genetic diversity in the genus Eleusine with DNA markers

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 757-763 ◽  
Author(s):  
Shanmukhaswami S. Salimath ◽  
Antonio C. de Oliveira ◽  
Jeffrey L. Bennetzen ◽  
Ian D. Godwin
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Finger Millet ◽  
Dna Marker ◽  
Fragment Length Polymorphism ◽  
Eleusine Coracana ◽  
Randomly Amplified Polymorphic Dna ◽  
Simple Sequence ◽  
Restriction Fragment Length

Finger millet (Eleusine coracana), an allotetraploid cereal, is widely cultivated in the arid and semiarid regions of the world. Three DNA marker techniques, restriction fragment length polymorphism (RFLP), randomly amplified polymorphic DNA (RAPD), and inter simple sequence repeat amplification (ISSR), were employed to analyze 22 accessions belonging to 5 species of Eleusine. An 8 probe – 3 enzyme RFLP combination, 18 RAPD primers, and 6 ISSR primers, respectively, revealed 14, 10, and 26% polymorphism in 17 accessions of E. coracana from Africa and Asia. These results indicated a very low level of DNA sequence variability in the finger millets but did allow each line to be distinguished. The different Eleusine species could be easily identified by DNA marker technology and the 16% intraspecific polymorphism exhibited by the two analyzed accessions of E. floccifolia suggested a much higher level of diversity in this species than in E. coracana. Between species, E. coracana and E. indica shared the most markers, while E. indica and E. tristachya shared a considerable number of markers, indicating that these three species form a close genetic assemblage within the Eleusine. Eleusine floccifolia and E. compressa were found to be the most divergent among the species examined. Comparison of RFLP, RAPD, and ISSR technologies, in terms of the quantity and quality of data output, indicated that ISSRs are particularly promising for the analysis of plant genome diversity.Key words: Eleusine coracana, finger millet, genome analysis, microsatellites, randomly amplified polymorphic DNA, restriction fragment length polymorphism, simple sequence repeats.

Physical mapping of restriction fragment length polymorphism (RFLP) markers in homoeologous groups 1 and 3 chromosomes of wheat by in situ hybridization

Genome ◽  
2001 ◽  
Vol 44 (3) ◽  
pp. 401-412 ◽  
Author(s):  
X -F. Ma ◽  
K Ross ◽  
J P Gustafson
Keyword(s):  
In Situ Hybridization ◽  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Physical Mapping ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Rflp Markers ◽  
Restriction Fragment Length

Using wheat ditelosomic lines and in situ hybridization of biotin-labelled DNA probes, 18 restriction fragment length polymorphism (RFLP) markers were physically located on homoeologous groups 1 and 3 chromosomes of wheat. Most of the markers hybridized to chromosome arms in a physical order concordant with the genetic maps. A majority of the markers studied were clustered in non-C-banded, distal euchromatic areas, indicating the presence of recombination hot spots and cold spots in those regions. However, on 1BS the markers were well dispersed, which could be due to the abundance of heterochromatin throughout the arm. An inversion between Xpsr653 and Xpsr953 was observed on 1AL. One new Xpsr688 locus, approximately 20–26% from the centromere, was found on 1AS and 1BS. The physical location of Xpsr170 on group 3 chromosomes probably represents an alternative to the loci on the genetic map. Finally, Xpsr313 was mapped to two physical loci on 1DL. Five markers were located to bins consistent with the deletion-based physical maps.Key words: wheat, physical mapping, in situ hybridization.

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