scholarly journals A comparative genetic diversity analysis in mungbean (Vigna radiata L.) using inter-simple sequence repeat (ISSR) and amplified fragment length polymorphism (AFLP)

2013 ◽  
Vol 12 (47) ◽  
pp. 6574-6582 ◽  
Author(s):  
Singh Renu ◽  
W. van Heusden Adriaan ◽  
C. Yadav Ram
Keyword(s):  
Genetic Diversity ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Simple Sequence Repeat ◽  
Fragment Length Polymorphism ◽  
Inter Simple Sequence Repeat ◽  
Diversity Analysis ◽  
Sequence Repeat ◽  
Genetic Diversity Analysis ◽  
Simple Sequence

Genetic diversity among Japanese indigenous common buckwheat (Fagopyrum esculentum) cultivars as determined from amplified fragment length polymorphism and simple sequence repeat markers and quantitative agronomic traits

Genome ◽  
2005 ◽  
Vol 48 (3) ◽  
pp. 367-377 ◽  
Author(s):  
Hiroyoshi Iwata ◽  
Kenta Imon ◽  
Yoshihiko Tsumura ◽  
Ryo Ohsawa
Keyword(s):  
Genetic Diversity ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Simple Sequence Repeat ◽  
Allelic Richness ◽  
Fragment Length Polymorphism ◽  
Agronomic Traits ◽  
Sequence Repeat ◽  
Common Buckwheat ◽  
Simple Sequence

We assessed the genetic diversity in Japanese indigenous common buckwheat (Fagopyrum esculentum) cultivars using amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers and investigated the relationships between the genetic diversity and agronomic traits. The average expected intracultivar hetero zygosity was 0.303 for AFLP and 0.819 for SSR. The differentiations among agroecotypes, among cultivars within an agroecotype, and among cultivars were small (0.002, 0.024, and 0.026 for SSR and 0.013, 0.013, and 0.026 for AFLP, respectively) but statistically significant from zero except for the SSR differentiation among agroecotypes. In principal coordinates analysis, cultivars within the same agroecotype tended to cluster, indicating that agroecotypes well reflected the genetic relationships among cultivars. In AFLP, the differentiation among the agroecotypes was more distinct than in SSR, and genetic distance showed a moderate correlation with the difference in quantitative traits, indicating that AFLP can resolve the relationships among cultivars with better resolution than SSR. By contrast, SSR may be more sensitive to demographic changes. Four of the five SSR markers showed a significant positive correlation (Kendall's τ = 0.382–0.607) between allelic richness and variation in flowering timing, indicating that cumulative bottleneck events have occurred during the population history, with a decline in the variation of photosensitivity of flowering.Key words: agroecotype, coefficient of gene differentiation, genetic distance, allelic richness, photosensitivity of flowering.

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