An isoleucine to leucine mutation in acetyl-CoA carboxylase confers herbicide resistance in wild oat

Genome ◽  
2002 ◽  
Vol 45 (6) ◽  
pp. 1049-1056 ◽  
Author(s):  
Michael J Christoffers ◽  
Michelle L Berg ◽  
Calvin G Messersmith
Keyword(s):  
Herbicide Resistance ◽  
Dna Sequences ◽  
Resistance Mutation ◽  
Triticum Aestivum L ◽  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Herbicide Resistant ◽  
Binding Domains ◽  
Substitution Site

Wild oat (Avena fatua L.) populations resistant to herbicides that inhibit acetyl-CoA carboxylase (ACCase; EC 6.4.1.2) represent an increasingly important weed control problem. The objective of this study was to determine the ACCase mutation responsible for herbicide resistance in a well-studied wild oat biotype (UM1). A 2039-bp region encompassing the carboxybiotin and acetyl-CoA binding domains of multifunctional plastidic ACCase was analyzed. DNA sequences representing three plastidic ACCase gene loci were isolated from both the resistant UM1 and a herbicide-susceptible biotype, consistent with the hexaploid nature of wild oat. Only one nonsynonymous point mutation was found among the resistant wild oat sequences, inferring an isoleucine to leucine substitution. The position of this substitution corresponds to residue 1769 of wheat (Triticum aestivum L.) plastidic ACCase (GenBank accession No. AF029895). Analysis of an F2 population derived from a cross between a herbicide-resistant and a susceptible biotype confirmed co-segregation of herbicide resistance with the mutated ACCase. We conclude that the isoleucine to leucine mutation is responsible for herbicide resistance in UM1 wild oat based on a comparison of the substitution site across species and ACCase types. While isoleucine is conserved among plastidic ACCases of herbicide-susceptible grasses, leucine is found in plastidic and cytosolic forms of multifunctional herbicide-resistant ACCase.Key words: acetyl-CoA carboxylase, herbicide resistance, mutation, wild oat.

ACCase-Inhibiting Herbicide-ResistantAvenaspp. Populations from the Western Australian Grain Belt

2012 ◽  
Vol 26 (1) ◽  
pp. 130-136 ◽  
Author(s):  
M. S. Ahmad-Hamdani ◽  
Mechelle J. Owen ◽  
Qin Yu ◽  
Stephen B. Powles
Keyword(s):  
Herbicide Resistance ◽  
Dose Response ◽  
Resistance Index ◽  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Grain Crops ◽  
High Level ◽  
Western Australian

Avenaspp. are world weeds with many cases of evolved herbicide resistance. In Australia,Avenaspp. (wild oat and sterile oat) are a major problem, especially in grain crops. Acetyl-CoA carboxylase (ACCase)–inhibiting herbicides have been used extensively since the late 1970s forAvenaspp. control. However, continued reliance on these herbicides has resulted in the evolution of resistantAvenaspp. populations. Resistance across many ACCase-inhibiting herbicides was characterized in fourAvenaspp. populations from the Western Australian grain belt. Dose–response experiments were conducted to determine the level of resistance to the aryloxyphenoxypropionates and cyclohexanediones and to the phenylpyrazoline herbicide pinoxaden. On the basis of resistance index values, all four resistant populations exhibited high-level diclofop resistance but varied in the level of resistance to other ACCase-inhibiting herbicides tested. It is evident thatAvenaspp. populations from the Western Australian grain belt have evolved resistance to a number of ACCase-inhibiting herbicides.

Characterization of cross-resistance patterns in acetyl-CoA carboxylase inhibitor resistant wild oat (Avena fatua)

Weed Science ◽  
1997 ◽  
Vol 45 (6) ◽  
pp. 750-755 ◽  
Author(s):  
Luc Bourgeois ◽  
Norm C. Kenkel ◽  
Ian N. Morrison
Keyword(s):  
Cluster Analysis ◽  
Principal Component ◽  
Petri Dish ◽  
Cross Resistance ◽  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Pairwise Correlation ◽  
Resistance Patterns

The purpose of this study was to determine cross-resistance patterns among wild oat lines resistant to acetyl-CoA carboxylase (ACCase) inhibitors and to determine which, if any, cross-resistant type was more common than another. Discriminatory concentrations of two aryloxyphenoxy-propionates (APP) and three cyclohexanediones (CHD) were determined using a petri-dish bioassay. These concentrations were then applied to 82 resistant wild oat lines identified in previous studies. In addition, two resistant standards (UM1 and UM33) and a susceptible standard (UM5) were included in the experiments. Coleoptile lengths expressed as percentages of untreated controls were used to assess the level of resistance to each herbicide. Large variations were observed among wild oat lines and herbicides. However, cluster analysis summarized the relationship between the five herbicides (variables) and the wild oat lines into three main cross-resistance types. Type A included wild oat lines with high resistance to APP herbicides and no or low resistance to CHD herbicides. Types B and C included those with low to moderate resistant and high levels of resistance to all five herbicides, respectively. Type C was the most common cross-resistance type. Relationships among herbicides were determined using pairwise correlation and principal component analysis (PCA). All correlations were high between APP herbicides and between CHD herbicides but not between APP and CHD herbicides. The first two axes of the PCA accounted for 88.4% of the total variance, with the first axis correlated to the CHD herbicides and the second axis correlated to the APP herbicides. In the PCA, wild oat lines were segregated into the three types identified in the cluster analysis. Although CHD and APP herbicides bind at the same region on the ACCase, resistant wild oat lines respond differently to them.

Resistant Acetyl-CoA Carboxylase is a Mechanism of Herbicide Resistance in a Biotype of Avena sterilis ssp. ludoviciana

1994 ◽  
Vol 35 (4) ◽  
pp. 627-635 ◽  
Author(s):  
Chanya Maneechote ◽  
Joseph A.M. Holtum ◽  
Christopher Preston ◽  
Stephen B. Powles
Keyword(s):  
Herbicide Resistance ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Avena Sterilis

Acetyl-CoA carboxylase overexpression in herbicide-resistant large crabgrass (Digitaria sanguinalis)

2017 ◽  
Vol 73 (11) ◽  
pp. 2227-2235 ◽  
Author(s):  
Martin Laforest ◽  
Brahim Soufiane ◽  
Marie-Josée Simard ◽  
Kristen Obeid ◽  
Eric Page ◽  
...  
Keyword(s):  
Acetyl Coa Carboxylase ◽  
Digitaria Sanguinalis ◽  
Acetyl Coa ◽  
Herbicide Resistant ◽  
Large Crabgrass

Inheritance of multiple herbicide resistance in wild oat (Avena fatua L.)

2006 ◽  
Vol 86 (1) ◽  
pp. 317-329 ◽  
Author(s):  
Jocelyn D Karlowsky ◽  
Anita L Brûlé-Babel ◽  
Lyle F Friesen ◽  
Rene C Van Acker ◽  
Gary H Crow
Keyword(s):  
Herbicide Resistance ◽  
Nuclear Gene ◽  
Avena Fatua ◽  
Multiple Resistance ◽  
Western Canada ◽  
Wild Oat ◽  
Herbicide Resistant ◽  
Insight Into ◽  
Resistance Genetics ◽  
Multiple Herbicide Resistance

To gain some insight into the surprisingly frequent occurrence of multiple herbicide resistant wild oat in western Canada, the inheritance of multiple herbicide resistance was studied in two wild oat (Avena fatua L.) populations, UMWO12-01 and UMWO12-03, from Manitoba, Canada. Both populations are resistant to each of three distinct herbicides, imazametha benz-methyl, flamprop-methyl, and fenoxaprop-p-ethyl (hereafter referred to as imazamethabenz, flamprop, and fenoxaprop-P, respectively). Crosses were made between each resistant (R) population and a susceptible (S) wild oat population (UM5) (R/S crosses), and between the two resistant populations (R/R crosses). Subsets of parental, F2 plants, and F2-derived F3 (F2:3) families were treated separately with each of the three herbicides and classified as R or S for individual plants, and homozygous R, segregating, or homozygous S for F2:3 families. F2 plants and F2:3 families from R/S crosses segregated in 3R:1S and 1 homozygous R:2 segregating:1 homozygous S ratios, respectively. These ratios indicate that a single dominant or semi-dominant nuclear gene controls resistance to each of these herbicides in each population. F2 plants and F2:3 families from R/R crosses segregated for resistance/susceptibility when treated with either imazamethabenz or flamprop. Therefore, the genes for resistance to these two herbicides are different in each R population. Individual F2:3 family response demonstrated that the genes were not independent of each other, indicating possible linkage between the genes for resistance to each herbicide. Genetic linkage could explain how the wild oat populations developed multiple resistance in the absence of selection by two of the herbicides, imazamethabenz and flamprop. Key words: Wild oat, Avena fatua, herbicide resistance, genetics of resistance, multiple resistance

scholarly journals Fenoxaprop-p-ethyl Susceptibility and Mutation Point Detection of Acetyl-CoA Carboxylase (ACCase) in Different Wild oat (Avena fatua L.) Populations from China

2019 ◽  
Author(s):  
Jun-jie Liu ◽  
Liuyang Lu ◽  
Bai-zhong Zhang ◽  
Xi-ling Chen
Keyword(s):  
Gene Expression ◽  
Critical Role ◽  
Enzyme Linked Immunosorbent Assay ◽  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Mutation Site ◽  
Multiple Sequence ◽  
Susceptible Population ◽  
In The Wild

AbstractTo explore resistant mechanism of wild oat to fenoxaprop-p-ethyl, the susceptibility of Acetyl-CoA Carboxylase (ACCase) from 24 wild oat populations to fenoxaprop-p-ethyl, the level of gene expression, and mutation site of ACCase were conducted. In vitro ACCase activities were solated and measured by enzyme-linked immunosorbent assay kit (ELISA) assays, the results indicated that the IC50 value of the ACCase of the most unsusceptible to fenoxaprop-p-ethyl in the wild oat population from Yexian2017 (W24) was 7206.557-fold compared to that of the ACCase of most susceptible to fenoxaprop-p-ethyl in the wild oat population from Queshan (W11). The differential expression of genes in wild oat treated by the IC50 fenoxaprop-p-ethyl concentration (6.9 mg/L) for 24 hours using RNA-seq, digital gene expression (DGE) profling was conducted. We found that 8 unigenes annotated as ACCase genes, 0 up-regulaed expression and 3 down-regulated expression were observed. The down-regulaed expressed ACCase was selected for qPCR in the relative susceptible population were significantly more suppressed than the three relative resistant populations. The mutations point of ACCase, Ile-1781-Leu, Trp-1999-Cys, Trp-2027-Cys, Ile-2041-Asn, Asp-2078-Gly, Cys-2088-Arg published were not found in the populations tested by multiple sequence alignment with a model complete ACCase sequence of Alopecurus myosuroides. These findings suggest that ACCase plays a critical role in the development of wild oat resistance to fenoxaprop-p-ethyl.

Selection and evolution of acetyl-CoA carboxylase (ACC)-inhibitor resistance in wild oat (Avena fatua L.) in a long-term alternative cropping systems study

2014 ◽  
Vol 94 (4) ◽  
pp. 727-731 ◽  
Author(s):  
Hugh J. Beckie ◽  
Eric N. Johnson ◽  
Julia Y. Leeson ◽  
Scott W. Shirriff ◽  
Arlen Kapiniak
Keyword(s):  
Cropping Systems ◽  
Cropping System ◽  
Avena Fatua ◽  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Evolution Of Resistance ◽  
Inhibitor Resistance ◽  
Herbicide Use

Beckie, H. J., Johnson, E. N., Leeson, J. Y., Shirriff, S. W. and Kapiniak, A. 2014. Selection and evolution of acetyl-CoA carboxylase (ACC)-inhibitor resistance in wild oat (Avena fatua L.) in a long-term alternative cropping systems study. Can. J. Plant Sci. 94: 727–731. In 2012, 18 yr after experiment establishment, wild oat from the spring wheat phase of seven of nine alternative cropping systems (each of three input levels applied to three levels of cropping diversity) were sampled and screened for ACC-inhibitor resistance. The frequency or level of resistance in wild oat was greatest in the diversified annual grains systems (42–60% of individuals), and lowest in the diversified annual perennial systems (<3%). The results of this study demonstrate the importance of perennial crops in slowing the selection and evolution of resistance in this weed. Moreover, annual cropping system diversity by itself is not enough to slow the evolution of ACC-inhibitor resistance in wild oat; cropping diversity must be linked with herbicide mode-of-action diversity and herbicide-use reduction.

Cross Resistance of Acetyl-coa Carboxylase (ACCase) Inhibitor–Resistant Wild Oat (Avena fatua) Biotypes in the Pacific Northwest

2008 ◽  
Vol 22 (1) ◽  
pp. 142-145 ◽  
Author(s):  
Ahmet Uludag ◽  
Kee Woong Park ◽  
Joshua Cannon ◽  
Carol A. Mallory-Smith
Keyword(s):  
Pacific Northwest ◽  
Avena Fatua ◽  
Cross Resistance ◽  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
The Pacific

Selecting for triallate resistance in wild oat

2000 ◽  
Vol 80 (3) ◽  
pp. 665-667 ◽  
Author(s):  
Hugh J. Beckie ◽  
Sakti Jana
Keyword(s):  
Triticum Aestivum ◽  
Key Words ◽  
Herbicide Resistance ◽  
Spring Wheat ◽  
Triticum Aestivum L ◽  
Avena Fatua ◽  
Wild Oat

Resistance in wild oat (Avena fatua L.) to triallate was monitored from 1996 to 1998 in plots of continuous spring wheat (Triticum aestivum L.) and wheat-fallow, with or without triallate applied from 1979 to 1997. Resistance in wild oat occurred after 18 yr where triallate was applied annually in continuous wheat, but not where triallate was applied 10 times in a wheat-fallow rotation over the same period. No resistance was detected in the continuous wheat or wheat-fallow plots where no herbicide was applied. Key words: Herbicide resistance, wild oat, triallate

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