scholarly journals Fenoxaprop-p-ethyl Susceptibility and Mutation Point Detection of Acetyl-CoA Carboxylase (ACCase) in Different Wild oat (Avena fatua L.) Populations from China

2019 ◽  
Author(s):  
Jun-jie Liu ◽  
Liuyang Lu ◽  
Bai-zhong Zhang ◽  
Xi-ling Chen
Keyword(s):  
Gene Expression ◽  
Critical Role ◽  
Enzyme Linked Immunosorbent Assay ◽  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Mutation Site ◽  
Multiple Sequence ◽  
Susceptible Population ◽  
In The Wild

AbstractTo explore resistant mechanism of wild oat to fenoxaprop-p-ethyl, the susceptibility of Acetyl-CoA Carboxylase (ACCase) from 24 wild oat populations to fenoxaprop-p-ethyl, the level of gene expression, and mutation site of ACCase were conducted. In vitro ACCase activities were solated and measured by enzyme-linked immunosorbent assay kit (ELISA) assays, the results indicated that the IC50 value of the ACCase of the most unsusceptible to fenoxaprop-p-ethyl in the wild oat population from Yexian2017 (W24) was 7206.557-fold compared to that of the ACCase of most susceptible to fenoxaprop-p-ethyl in the wild oat population from Queshan (W11). The differential expression of genes in wild oat treated by the IC50 fenoxaprop-p-ethyl concentration (6.9 mg/L) for 24 hours using RNA-seq, digital gene expression (DGE) profling was conducted. We found that 8 unigenes annotated as ACCase genes, 0 up-regulaed expression and 3 down-regulated expression were observed. The down-regulaed expressed ACCase was selected for qPCR in the relative susceptible population were significantly more suppressed than the three relative resistant populations. The mutations point of ACCase, Ile-1781-Leu, Trp-1999-Cys, Trp-2027-Cys, Ile-2041-Asn, Asp-2078-Gly, Cys-2088-Arg published were not found in the populations tested by multiple sequence alignment with a model complete ACCase sequence of Alopecurus myosuroides. These findings suggest that ACCase plays a critical role in the development of wild oat resistance to fenoxaprop-p-ethyl.

Two Distinct Alleles Encode for Acetyl-CoA Carboxylase Inhibitor Resistance in Wild Oat (Avena fatua)

Weed Science ◽  
1996 ◽  
Vol 44 (3) ◽  
pp. 476-481 ◽  
Author(s):  
Bruce G. Murray ◽  
Anita L. Brûlé-Babel ◽  
Ian N. Morrison
Keyword(s):  
Gene Locus ◽  
Nuclear Gene ◽  
Cross Resistance ◽  
Resistance Pattern ◽  
Resistance Allele ◽  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Susceptible Population ◽  
In The Wild ◽  
Inhibitor Resistance

The objectives of this study were to determine the inheritance of aryloxyphenoxypropionate (APP) resistance in the wild oat population UM33 and to determine the genetic relationship between resistance in UM33 and another population, UM1, which has a different cross-resistance pattern. Reciprocal crosses were made between UM33 and a susceptible population UM5, and between UM33 and UM1. Initial screenings of F1and F2Is populations derived from crosses between UM33 and UM5 were conducted over a range of fenoxaprop-P rates to determine a discriminatory dosage. F2populations and F2-derived F3families were then screened at this dosage (1200 g ai ha−1) to determine segregation patterns. Results from reciprocal UM33 x UM5 F1dose-response experiments, and F2and F2-derived F3segregation experiments indicated that UM33 resistance to fenoxaprop-P was governed by a single, partially dominant nuclear gene system. To determine if resistance in UM1 and UM33 results from alterations at the same gene locus, 584 F2plants derived from reciprocal UM33 x UM1 crosses were screened with 150 g ha−1fenoxaprop-P. This dosage was sufficient to kill susceptible plants (UM5), but was not sufficient to kill plants with a resistance allele from either parent. None of the treated F2plants exhibited injury or death, indicating that UM1 and UM33 resistance genes did not segregate independently. From this it was concluded that resistance in both populations is encoded at the same gene locus.

ACCase-Inhibiting Herbicide-ResistantAvenaspp. Populations from the Western Australian Grain Belt

2012 ◽  
Vol 26 (1) ◽  
pp. 130-136 ◽  
Author(s):  
M. S. Ahmad-Hamdani ◽  
Mechelle J. Owen ◽  
Qin Yu ◽  
Stephen B. Powles
Keyword(s):  
Herbicide Resistance ◽  
Dose Response ◽  
Resistance Index ◽  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Grain Crops ◽  
High Level ◽  
Western Australian

Avenaspp. are world weeds with many cases of evolved herbicide resistance. In Australia,Avenaspp. (wild oat and sterile oat) are a major problem, especially in grain crops. Acetyl-CoA carboxylase (ACCase)–inhibiting herbicides have been used extensively since the late 1970s forAvenaspp. control. However, continued reliance on these herbicides has resulted in the evolution of resistantAvenaspp. populations. Resistance across many ACCase-inhibiting herbicides was characterized in fourAvenaspp. populations from the Western Australian grain belt. Dose–response experiments were conducted to determine the level of resistance to the aryloxyphenoxypropionates and cyclohexanediones and to the phenylpyrazoline herbicide pinoxaden. On the basis of resistance index values, all four resistant populations exhibited high-level diclofop resistance but varied in the level of resistance to other ACCase-inhibiting herbicides tested. It is evident thatAvenaspp. populations from the Western Australian grain belt have evolved resistance to a number of ACCase-inhibiting herbicides.

Expression Profile of Acetyl CoA Carboxylase Beta (ACACB) Gene during the Pre and Post-hatch Period in Chicken

2021 ◽  
Author(s):  
Ch. Shiva Prasad ◽  
R. Vinoo ◽  
R.N. Chatterjee ◽  
M. Muralidhar ◽  
D. Narendranath ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acid ◽  
Expression Pattern ◽  
Fatty Acyl ◽  
Acid Oxidation ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Layer Chicken ◽  
Differential Expression Pattern ◽  
Long Chain

Background: Acetyl-CoA Carboxylase Beta (ACACB) plays a key role in fatty acid oxidation and was known to be involved in production of very-long-chain fatty acid and other compounds needed for proper development. This gene is mainly expressed in the tissues of heart, muscle, liver and colon. It chiefly involved in the production of malonyl-coA, a potent inhibitor of carnitine palmitoyl transferase I (CPT-I) enzyme needed in transport of long-chain fatty acyl-coAs to the mitochondria for β-oxidation.Methods: The present study was conducted to explore the expression pattern of the ACACB gene in breast muscle tissue during pre-hatch embryonic day (ED) 5th to 18th and post-hatch (18th, 22nd and 40th week of age) periods of White leghorn (IWI line) by using Quantitative real-time PCR (qPCR). Then, fold change of ACACB gene expression was calculated.Result: Our study showed that the ACACB gene expression was down-regulated during embryonic stages from ED6 to ED18. The gene expression was also down-regulated during adult stages i.e. on 22nd and 40th week of age. This result indicated that the initial expression of the ACACB gene is required for embryo development and during adult periods, low gene expression leads to the less fat deposition in muscle of layer chicken. Finally, it can be concluded that there was a differential expression pattern of the ACACB gene during the pre-hatch embryonic and post-hatch adult periods to mitigate varied requirements of lipids during different physiological stages in layer chicken.

Soy Protein Suppresses Gene Expression of Acetyl-CoA Carboxylase Alpha from Promoter PI in Rat Liver

2006 ◽  
Vol 70 (4) ◽  
pp. 843-849 ◽  
Author(s):  
Hisa AOKI ◽  
Kumi KIMURA ◽  
Kiharu IGARASHI ◽  
Asako TAKENAKA
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
Soy Protein ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa

Characterization of cross-resistance patterns in acetyl-CoA carboxylase inhibitor resistant wild oat (Avena fatua)

Weed Science ◽  
1997 ◽  
Vol 45 (6) ◽  
pp. 750-755 ◽  
Author(s):  
Luc Bourgeois ◽  
Norm C. Kenkel ◽  
Ian N. Morrison
Keyword(s):  
Cluster Analysis ◽  
Principal Component ◽  
Petri Dish ◽  
Cross Resistance ◽  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Pairwise Correlation ◽  
Resistance Patterns

The purpose of this study was to determine cross-resistance patterns among wild oat lines resistant to acetyl-CoA carboxylase (ACCase) inhibitors and to determine which, if any, cross-resistant type was more common than another. Discriminatory concentrations of two aryloxyphenoxy-propionates (APP) and three cyclohexanediones (CHD) were determined using a petri-dish bioassay. These concentrations were then applied to 82 resistant wild oat lines identified in previous studies. In addition, two resistant standards (UM1 and UM33) and a susceptible standard (UM5) were included in the experiments. Coleoptile lengths expressed as percentages of untreated controls were used to assess the level of resistance to each herbicide. Large variations were observed among wild oat lines and herbicides. However, cluster analysis summarized the relationship between the five herbicides (variables) and the wild oat lines into three main cross-resistance types. Type A included wild oat lines with high resistance to APP herbicides and no or low resistance to CHD herbicides. Types B and C included those with low to moderate resistant and high levels of resistance to all five herbicides, respectively. Type C was the most common cross-resistance type. Relationships among herbicides were determined using pairwise correlation and principal component analysis (PCA). All correlations were high between APP herbicides and between CHD herbicides but not between APP and CHD herbicides. The first two axes of the PCA accounted for 88.4% of the total variance, with the first axis correlated to the CHD herbicides and the second axis correlated to the APP herbicides. In the PCA, wild oat lines were segregated into the three types identified in the cluster analysis. Although CHD and APP herbicides bind at the same region on the ACCase, resistant wild oat lines respond differently to them.

Inheritance of Acetyl-CoA Carboxylase Inhibitor Resistance in Wild Oat (Avena fatua)

Weed Science ◽  
1995 ◽  
Vol 43 (2) ◽  
pp. 233-238 ◽  
Author(s):  
Bruce G. Murray ◽  
Ian N. Morrison ◽  
Anita L. Brûlé-Babel
Keyword(s):  
Nuclear Gene ◽  
Avena Fatua ◽  
Wild Oat ◽  
Acetyl Coa Carboxylase ◽  
Reciprocal Crosses ◽  
Characteristic Response ◽  
Wide Range ◽  
Segregation Ratios ◽  
In The Wild ◽  
Inhibitor Resistance

Resistance to fenoxaprop-P and other aryloxyphenoxypropionate and cyclohexanedione herbicides in the wild oat population, UM1, is controlled by a single, partially dominant, nuclear gene. In arriving at this conclusion, parents, F1hybrids, and F2plants derived from reciprocal crosses between UM1 and a susceptible wild oat line, UM5, were treated with fenoxaprop-P over a wide range of dosages. Based on these experiments, a dosage of 400 g ai ha−1fenoxaprop-P was selected to discriminate between three response types. At this dosage, susceptible plants were killed and resistant plants were unaffected, whereas plants characterized as intermediate in response were injured but recovered. Treated F2plants segregated in a 1:2:1 (R, I, S) ratio, indicative of single nuclear gene inheritance. This was confirmed by selfing F2plants and screening several F3families. Families derived from intermediate F2plants segregated for the three characteristic response types, whereas those derived from resistant F2plants were uniformly resistant. Chisquare analysis indicated the F2segregation ratios fit those expected for a single partially dominant nuclear gene system. In addition, F2populations from both crosses were screened with a mixture of fenoxaprop-Pand sethoxydim. The dosages of both herbicides (150 g ai ha−1fenoxaprop-P and 100 g ha−1sethoxydim) were sufficient to control only susceptible plants. Treated F2populations segregated in a 3:1 (R:S) pattern, thereby confirming that resistance to the two chemically unrelated herbicides results from the same gene alteration.

Regulation of mammalian acetyl-CoA carboxylase

2002 ◽  
Vol 30 (6) ◽  
pp. 1059-1064 ◽  
Author(s):  
M. R. Munday
Keyword(s):  
Fatty Acid ◽  
Protein Kinase ◽  
Critical Role ◽  
Physiological Role ◽  
Acid Synthesis ◽  
Acid Oxidation ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Dependent Protein ◽  
Amp Activated Protein Kinase

Acetyl-CoA carboxylase (ACC) plays a critical role in the regulation of fatty acid metabolism and its two isoforms, ACCα and ACCβ, appear to have distinct functions in the control of fatty acid synthesis and fatty acid oxidation, respectively. They are regulated by similar short-term mechanisms of allosteric activation by citrate, and reversible phosphorylation and inactivation, and there is clearly interaction between these mechanisms. AMP-activated protein kinase is the important physiological ACC kinase for both isoforms and yet there is a potential physiological role for cAMP-dependent protein kinase in the hormonally mediated inactivation of ACCα, and phosphorylation of ACCβ in its unique N-terminus.

scholarly journals Comparative RNA-sequencing profiled the differential gene expression of liver in response to acetyl-CoA carboxylase inhibitor GS-0976 in a mouse model of NASH

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e8115
Author(s):  
Ying Lu ◽  
Xiaolan Su ◽  
Manyu Zhao ◽  
Qianru Zhang ◽  
Chuang Liu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mouse Model ◽  
Rna Sequencing ◽  
Hepatic Steatosis ◽  
Differential Gene Expression ◽  
Receptor Interaction ◽  
Therapeutic Effects ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Differential Gene

Background Non-alcoholic steatohepatitis (NASH) is a progressive liver disease characterized by hepatic steatosis, lobular inflammation and fibrosis. Acetyl-CoA carboxylase (ACC) isoform 1 and 2 involved in de novo lipogenesis (DNL) and fatty acid oxidation have been identified as a therapeutic target in NASH. GS-0976, the inhibitor of ACC1 and ACC2, has achieved favorable therapeutic effects in clinical trials with NASH. The purpose of this study was to explore the transcriptional alterations regulated by GS-0976 in NASH. Methods C57BL/6 mice were fed on a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) or normal diet for 12 weeks. Mice were treated with or without GS-0976 (3 mg/kg per day) in the last 8 weeks. Oil Red O, Haematoxylin-eosin (H & E), and Sirius Red were used to evaluate hepatic steatosis, inflammation and fibrosis. The comparative RNA-sequencing was conducted to analyse the hepatic gene expression profiles in mice. Reverse transcription–polymerase chain reaction analysis was performed to validate the differential expression of representative genes. Results GS-0976 attenuated the steatosis, inflammation, and fibrosis of NASH in CDAHFD mouse model. High-throughput sequencing and differential gene expression analysis showed that there were 516 up-regulated genes and 525 down-regulated genes after GS-0976 treatment. Genes involved in the metabolic process, extracellular matrix formation, immune response, and angiogenesis were significantly enriched. The “Metabolic pathways” and “ECM-receptor interaction” pathways were the most significantly enriched KEGG pathways in the up-regulated and down-regulated differentially expressed genes (DEGs), respectively. Conclusions Transcriptome analysis showed that GS-0976 could regulate the expression of genes related to metabolism, inflammation and fibrosis in NASH. The global transcriptomic changes in gene expression promote the further understanding for the inhibition mechanisms of GS-0976 in NASH.

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