Erratum: Population structure of a lodgepole pine (Pinus contorta) and jack pine (P. banksiana) complex as revealed by random amplified polymorphic DNA

Genome ◽  
2002 ◽  
Vol 45 (4) ◽  
pp. 784-785
Author(s):  
Terrance Z Ye ◽  
Rong-Cai Yang ◽  
Francis C Yeh
Keyword(s):  
Population Structure ◽  
Pinus Contorta ◽  
Lodgepole Pine ◽  
Random Amplified Polymorphic Dna ◽  
Jack Pine

Population structure of a lodgepole pine (Pinus contorta) and jack pine (P. banksiana) complex as revealed by random amplified polymorphic DNA

Genome ◽  
2002 ◽  
Vol 45 (3) ◽  
pp. 530-540 ◽  
Author(s):  
Terrance Z Ye ◽  
Rong-Cai Yang ◽  
Francis C Yeh
Keyword(s):  
Population Structure ◽  
Pinus Banksiana ◽  
Pinus Contorta ◽  
Lodgepole Pine ◽  
Random Amplified Polymorphic Dna ◽  
Introgressive Hybridization ◽  
Jack Pine ◽  
Natural Hybridization ◽  
Secondary Contact ◽  
Putative Hybrid

We studied the population structure of a lodgepole (Pinus contorta Dougl.) and jack pine (Pinus banksiana Lamb.) complex in west central Alberta and neighboring areas by assessing random amplified polymorphic DNA (RAPD) variability in 23 lodgepole pine, 9 jack pine, and 8 putative hybrid populations. Of 200 random primers screened, 10 that amplified 39 sharp and reproducible RAPDs were chosen for the study. None of the 39 RAPDs were unique to the parental species. RAPD diversity ranged from 0.085 to 0.190 among populations and averaged 0.143 for lodgepole pine, 0.156 for jack pine, 0.152 for hybrids, and 0.148 for all 40 populations. The estimated population differentiation based on GST was 0.168 for hybrids, 0.162 for lodgepole pine, 0.155 for jack pine, and 0.247 across all 40 populations. Cluster analysis of genetic distances generally separated jack pine from lodgepole pine and hybrids, but no division could be identified that further separated lodgepole pine from hybrids. The observed weak to mild trend of "introgression by distance" in the complex and neighbouring areas was consistent with the view that introgressive hybridization between lodgepole and jack pines within and outside the hybrid zone may have been through secondary contact and primary intergradation, respectively.Key words: introgression, lodgepole–jack pine complex, natural hybridization, random amplified polymorphic DNA.

Random amplified polymorphic DNA variability among geographic isolates of western gall rust fungus in Canada

2001 ◽  
Vol 31 (8) ◽  
pp. 1304-1311 ◽  
Author(s):  
Changxi Li ◽  
Francis C Yeh ◽  
Yasu Hiratsuka
Keyword(s):  
Pinus Banksiana ◽  
Pinus Contorta ◽  
Lodgepole Pine ◽  
Random Amplified Polymorphic Dna ◽  
Rust Fungus ◽  
Jack Pine ◽  
Fungal Isolates ◽  
Rapd Pattern ◽  
Endocronartium Harknessii ◽  
East West

Geographic variability among western gall rust (WGR) fungus (Endocronartium harknessii (J.P. Moore) Y. Hiratsuka) was studied by random amplified polymorphic DNA (RAPD). Samples were taken from lodgepole pine (Pinus contorta Dougl. ex Loud. var. latifolia Engelm.) host at four locations in British Columbia and Alberta and from jack pine (Pinus banksiana Lamb.) host at nine locations in Alberta, Saskatchewan, Manitoba, and Ontario. Of 90 random oligonucleotide primers screened, 9 were chosen for analysis. These nine primers consistently amplified 41 sharp and reproducible RAPDs (fragments) of the WGR fungal isolates over several independent runs. Eighteen of the 41 RAPDs were polymorphic (showing the presence of both marker and null phenotypes), of which 15 could discriminate WGR isolates of lodgepole pine hosts from jack pine ones. Of these 15 RAPDs, five were unique to isolates of lodgepole and five to jack pine. The remaining five RAPDs were significantly heterogeneous in the RAPD frequency between WGR isolates of the two host origins. The RAPD pattern of WGR isolates from lodgepole pine was uniform. However, isolates from jack pine differed significantly in the frequency of four RAPDs among locations, with an east-west trend of decreasing similarity in RAPD. Analysis of molecular variance apportioned 76.3, 14.4, and 9.3% of the total RAPD variability to differences among hosts, to differences among locations within hosts, and to differences within locations, respectively. The large differentiation between WGR fungal isolates sampled in lodgepole pine and jack pine hosts might suggest that selective pressure for host specificity in sampled populations was strong.

Stability of resistance to western gall rust and needle cast in lodgepole pine provenances

1998 ◽  
Vol 28 (3) ◽  
pp. 439-449 ◽  
Author(s):  
Harry X Wu ◽  
Cheng C Ying
Keyword(s):  
Pinus Banksiana ◽  
Pinus Contorta ◽  
Lodgepole Pine ◽  
Genetic Selection ◽  
Jack Pine ◽  
Needle Cast ◽  
Geographic Patterns ◽  
Endocronartium Harknessii ◽  
Stability Of Resistance ◽  
Site Evaluation

Stability of 76 interior lodgepole pine (Pinus contorta ssp. latifolia Engelm.) provenances in resistance to western gall rust (Endocronartium harknessii (J.P. More) Y. Hiratsuka) and needle cast (Lophodermella concolor (Dearn.) Darker) was investigated from 19 and 23 sites in the British Columbia interior, respectively. Provenances, sites, and provenance by site interaction had significant effects on severity level of infection of both diseases. Susceptible provenances contributed mainly to the interaction. The resistant provenances to both diseases were very stable and essentially homeostatic across sites (regression coefficient approaching 0). Resistant provenances were concentrated in the jack pine (Pinus banksiana Lamb.) - lodgepole pine hybrid zone and adjacent areas, and provenances from the low-elevation interior wetbelt were also very resistant to needle cast. Geographic patterns of provenance variation revealed that the closer a lodgepole pine provenance is to the limit of jack pine distribution, the higher and more stable is its resistance to western gall rust and needle cast. The current multiple-site evaluation supports for the hypothesis that jack pine introgression influences pest defence in lodgepole pine and suggests genetic selection can be effective.

scholarly journals Shoot Blight of Lodgepole Pine Seedlings in Nebraska Caused by Sphaeropsis sapinea

Plant Disease ◽  
1997 ◽  
Vol 81 (3) ◽  
pp. 311-311 ◽  
Author(s):  
G. R. Stanosz ◽  
D. L. Kimbler
Keyword(s):  
Pinus Contorta ◽  
Lodgepole Pine ◽  
Random Amplified Polymorphic Dna ◽  
Stem Canker ◽  
Shoot Tips ◽  
State College ◽  
Pine Seedlings ◽  
Pure Cultures ◽  
Shoot Blight ◽  
Sphaeropsis Sapinea

Shoot blight was observed on lodgepole pine (Pinus contorta Douglas ex Loud.) seedlings in fall 1994 at the USDA Forest Service Bessey Tree Nursery, Halsey, NE. Shoots were stunted, cankered, and sometimes curled at the tips, and bore brown needles that often had been killed before full elongation. Pycnidia were present on necrotic needles and stems. Conidia from these pycnidia produced pure cultures of Sphaeropsis sapinea (Fr.:Fr.) Dyko & Sutton in Sutton. The fungus was identified based on cultural, pycnidial, and conidial characteristics and comparison with known isolates. Also, random amplified polymorphic DNA markers of isolates were consistent with those of the A morphotype of S. sapinea (1). The ability of each of the two recognized morphotypes of S. sapinea to cause shoot blight of lodgepole pine was tested by inoculation of potted, 2-year-old seedlings in a greenhouse. Elongating terminal shoots were wounded by removing a needle pair approximately 1 cm below the shoot apex. A plug cut from an actively growing culture on water agar (WA) was placed fungus-side-down on the wound. The two isolates used (128, A morphotype; and 124, B morphotype) are representative of larger collections of these two morphotypes for which aggressiveness has been compared (2). Noncolonized WA plugs were placed on similarly wounded control seedlings. Nonwounded control seedlings also were used. Parafilm was wrapped around the shoots to hold the agar plugs in place and was removed 1 week later. Each treatment was applied to 10 seedlings in each of five completely randomized replicates. After 4 weeks, the condition (living or dead) of shoot tips was recorded, and the length of necrotic stem (canker) below the point of inoculation was measured to the nearest 0.25 cm. Segments of shoots were harvested, surface sterilized, and incubated to determine the presence of the pathogen. Analyses of variance were performed with Minitab for Windows version 10.1 software (Minitab Inc., State College, PA). Seedlings inoculated with either morphotype developed symptoms resembling those observed in the nursery. Neither incidence of shoot tip death nor severity (as indicated by canker length) differed greatly according to isolate morphotype. The means for number of shoot tips killed by the A and B isolates were 8.8 and 6.4 (out of 10), respectively (P = 0.07). The means for lengths of cankers produced by the A and B isolates were 4.9 and 3.8 cm, respectively (P = 0.10). Among controls, only one wounded seedling developed any symptoms. The pathogen was identified on incubated shoot segments from inoculated seedlings, but not those of control seedlings. This is the first report of S. sapinea as a cause of shoot blight of lodgepole pine in the U.S., and it associates the A morphotype with damage observed in the Bessey nursery. Further, the ability of the B morphotype to cause disease of lodgepole pine is established. Results of inoculations contrast with those obtained with red pine (Pinus resinosa Aiton) seedlings, on which the B morphotype was not aggressive (2). Susceptibility of lodgepole pine seedlings to both A and B morphotypes of S. sapinea, however, is similar to that exhibited by the related species, jack pine (P. banksiana Lamb.) (2). These species are classified in subsection “contortae” of the genus Pinus. Both morphotypes of S. sapinea should be considered as potential causes of shoot blight encountered on lodgepole pine. References: (1) G. R. Stanosz et al. Plant Dis. 80:1175, 1996. (2) J. T. Blodgett and G. R. Stanosz. Plant Dis. 81:143, 1997.

Population differentiation of the lodgepole pine (Pinus contorta) and jack pine (Pinus banksiana) complex in Alberta: growth, survival, and responses to climate

2007 ◽  
Vol 85 (6) ◽  
pp. 545-556 ◽  
Author(s):  
Deogratias M. Rweyongeza ◽  
Narinder K. Dhir ◽  
Leonard K. Barnhardt ◽  
Christine Hansen ◽  
Rong-Cai Yang
Keyword(s):  
Pinus Banksiana ◽  
Pinus Contorta ◽  
Lodgepole Pine ◽  
Jack Pine ◽  
Mahalanobis Distances ◽  
Growth And Survival ◽  
Population Distributions ◽  
Total Variability ◽  
Clustering Pattern ◽  
High Elevations

Growth and survival of 33 populations from a species complex involving interior lodgepole pine ( Pinus contorta Dougl. ex Loud. var. latifolia Engelm.) and jack pine ( Pinus banksiana Lamb.) and their natural hybrids in Alberta were evaluated at ages 5, 10, and 15 years in eight test sites across Alberta. We determined population differentiations by estimating Mahalanobis distances between populations from the canonical discriminant analysis of the total variability and by calculating dissimilarity indexes between populations from the quadratic regression of overall growth and survival on the overall climate. The grouping of the populations based on the Mahalanobis distances showed that most jack pine populations could be separated from lodgepole and hybrid populations, but no further subdivision was possible to distinguish lodgepole from hybrid populations. This clustering pattern was remarkably similar to the grouping based on molecular markers as shown in our earlier study. This pattern of grouping is best explained by a clear elevational demarcation between jack pine at low elevations and lodgepole pine and hybrids at midrange and high elevations. The grouping of the populations based on the dissimilarity indexes revealed a somewhat contrasting pattern; most lodgepole pine populations were in one group, whereas jack pine and hybrid populations were mixed up in the other group. The two contrasting patterns of grouping suggest that nonclimatic factors such as edaphic preference and habitat disturbances are also important in determining population distributions and niche spaces in the lodgepole – jack pine complex.

scholarly journals Representatives of thePine Family (Pinaceae Lindl.) of the North American Flora in the Collection of the Dendrological Garden named after V.N. Nilov (NRIF)

2021 ◽  
pp. 36-54
Author(s):  
Natalia Demidova ◽  
◽  
Tatiana Durkina ◽  
Liudmila Gogoleva ◽  
Natalia Vasiljeva ◽  
...  
Keyword(s):  
North American ◽  
Pinus Contorta ◽  
Lodgepole Pine ◽  
Abies Balsamea ◽  
Jack Pine ◽  
Douglas Fir ◽  
The North ◽  
European North ◽  
Northern Regions ◽  
Natural Range

The article presents the results of the introduction of the North American flora woody species of the pine family (Pinaceae Lindl.) to the European North of Russia. The research purpose is to select the most valuable specimens for introduction into northern conditions from the tree species collection. The research has involved experimental, calculation, analytical, and comparative methods. The conclusion on the result of the introduction of a particular plant was based on the materials of long-term phenological observations, which were carried out according to the method of botanical gardens improved for the conditions of the European North of Russia. As a result of the introduction of North American species of the Pinaceae Lindl. family to the region, a large-scale test of foreign conifers was carried out for the first time; a collection of 2 species of fir, 4 species and 3 forms of spruce, 2 species of pine, and 1 species of Douglas fir was created. Most attention was paid to the introduction of species of the genus Pinus. Positive results of cultivation of Pinus contorta Loud. var. latifolia S. Wats. in the Dendrological garden allowed us to select it for further introduction testing in experimental plantations of the region. On plantations located in the Arkhangelsk and Vologda regions and the Komi Republic, 104.2 ths seedlings were grown from the seed samples, mostly from the northern natural range of this pine. Unfortunately, not all of the breeding material for the North American pine species tested was derived from natural growth sites, which puts into question some of the unsatisfactory results. Therefore, for Jack pine and Weymouth pine, it is advisable to use in testing additional samples from the most northern regions of their natural ranges in Canada. Many of these species are of both scientific and practical interest for forestry. To assess the economic value of those introduced species, adaptive capabilities of which in the conditions of the European North according to the results of studies are not in doubt, it is advisable to lay out pilot plantations in the region, similar to the already created plantings of North American lodgepole pine (Pinus contorta Loud. var. latifolia S. Wats.). The concentration of different species like balsam fir (Abies balsamea Mill.), Fraser fir (Abies fraseri (Pursch.) Poir.), American larch (Larix laricina (Du Roi) K.Koch.), Canadian spruce (Picea glauca Voss.), Engelmann’s spruce (Picea engelmannii Engelm.), black spruce (Picea mariana Britt.), Jack pine (Pinus banksiana Lamb.), lodgepole pine (Pinus contorta Loud. var. latifolia S. Wats.), and Douglas fir (Pseudotsuga menziesii Mirb.), involving several seed origins from the most northern regions of its natural range for each, in 2-3 forest areas will allow creating a valuable introduction object; scientific and practical importance of those mentioned cannot be overestimated.

Monoterpene and morphological variation and hybridization of Pinus contorta and P. banksiana in Alberta

1985 ◽  
Vol 63 (2) ◽  
pp. 201-210 ◽  
Author(s):  
John C. Pollack ◽  
Bruce P. Dancik
Keyword(s):  
Multivariate Analysis ◽  
Pinus Contorta ◽  
Lodgepole Pine ◽  
Morphological Characters ◽  
Jack Pine ◽  
Putative Hybrid ◽  
Front Range ◽  
Needle Length ◽  
Pine Stands ◽  
Monoterpene Composition

Five xylem oleoresin monoterpenes and 17 morphological characters of trees in four pine stands in Alberta were measured to delineate pure lodgepole pine (Pinus contorta Dougl. var. latifolia Engelm.) from jack pine (P. banksiana Lamb.) in a region where hybridization commonly has been reported. Multivariate analysis determined α-pinene, β-phellandrene, and needle length best separated the taxa. The monoterpene composition of two putative hybrid stands at Onoway and Devon closely resembled that of jack pine. Analysis of the four major monoterpenes in an additional 10 stands allowed the identification of a transition zone between jack pine and lodgepole pine stands, which occurred farther west than previously reported and contained stands of highly variable monoterpene types. Trees in this region morphologically resembled lodgepole pine. The presence of variant monoterpene types in Front Range lodgepole pine stands and their absence from the Cypress Hills provide evidence for introgression of jack pine into lodgepole pine. No evidence of gene flow from lodgepole pine to jack pine was found. The two putative hybrid stands at Onoway and Devon probably consisted mostly of jack pine, with a small complement of hybrids.

Best of both worlds: hybrids of two commercially important pines (Pinus contorta × P. banksiana) combine increased growth potential and high drought tolerance

2021 ◽  
Author(s):  
Simon Bockstette ◽  
Raul de la Mata ◽  
Barb R. Thomas
Keyword(s):  
Pinus Contorta ◽  
Mountain Pine Beetle ◽  
Lodgepole Pine ◽  
Seed Orchard ◽  
Jack Pine ◽  
Snp Markers ◽  
Growth Potential ◽  
Pine Seedlings ◽  
Drought Resistant ◽  
Pine Hybrids

Over 18 million hectares of lodgepole pine forests have been affected by a massive mountain pine beetle outbreak in western Canada. The ensuing need for reforestation compels forest managers to deploy more drought-resistant stock in anticipation of a hotter and drier future climate. Recent studies suggest that naturally occurring lodgepole pine × jack pine hybrids may be more drought-resistant than pure lodgepole pine. We used single nucleotide polymorphism (SNP) markers to determine the level of jack pine introgression present in the region J lodgepole pine seed orchard, in northwest Alberta, and identified six hybrid genotypes with 10-18% jack pine introgression for further study. We compared the performance of open-pollinated seedlings of lodgepole, jack and hybrid pines under well-watered and drought conditions in a greenhouse experiment. Seedlings were subjected to repeated drought cycles, withholding water for either two or three weeks. We found that hybrid pines grew significantly faster than lodgepole pine under well-watered conditions while retaining the high tolerance to prolonged drought exhibited by lodgepole pine seedlings, a particularly beneficial combination of traits during the critical stage of establishment. We therefore suggest the deployment of hybrid lodgepole pine as a strategy to promote adaptation to hotter and drier future climates.

scholarly journals Paternally inherited chloroplast polymorphism in Pinus: estimation of diversity and population subdivision, and tests of disequilibrium with a maternally inherited mitochondrial polymorphism.

Genetics ◽  
1994 ◽  
Vol 136 (3) ◽  
pp. 1187-1194
Author(s):  
J Dong ◽  
D B Wagner
Keyword(s):  
Chloroplast Dna ◽  
Restriction Fragment ◽  
Pinus Banksiana ◽  
Pinus Contorta ◽  
Lodgepole Pine ◽  
Natural Populations ◽  
Jack Pine ◽  
Convincing Evidence ◽  
Population Subdivision ◽  
Dna Restriction

Abstract We have surveyed a chloroplast DNA restriction fragment length polymorphism in 745 individuals, distributed rangewide in eight allopatric natural populations of jack pine (Pinus banksiana Lamb.) and eight allopatric natural populations of lodgepole pine (Pinus contorta Dougl.). The polymorphic region of the chloroplast genome is located near duplicated psbA genes. Fourteen length variants were found in the survey, and these variants distinguished the two species qualitatively. Variant diversities were high in both species (hes = 0.43 in jack pine; hes = 0.44 in lodgepole pine). Population subdivision was weak within and among lodgepole pine subspecies and in jack pine (i.e., theta values were less than 0.05). This weak subdivision is compatible with theoretical predictions for paternally inherited markers in wind-pollinated outcrossers, as well as for polymorphisms with high length mutation rates. If these populations are at a drift-migration equilibrium, the chloroplast DNA restriction fragment data and previous mitochondrial frequency data from the same individuals are consistent with gene flow that is differential through seeds and pollen. The new data have permitted the first empirical tests of disequilibrium between maternally and paternally inherited factors. As expected, these tests failed to detect convincing evidence of nonrandom association between chloroplast and mitochondrial variants.

Susceptibility of Pinus contorta - Pinus banksiana complex to Endocronartium harknessii: host-pathogen interactions

1999 ◽  
Vol 77 (7) ◽  
pp. 1035-1043 ◽  
Author(s):  
Rong-Cai Yang ◽  
Zhihong Ye ◽  
Yasu Hiratsuka
Keyword(s):  
Pinus Banksiana ◽  
Pinus Contorta ◽  
Lodgepole Pine ◽  
Rust Fungus ◽  
Jack Pine ◽  
Natural Hybridization ◽  
Host Group ◽  
Group Interactions ◽  
Endocronartium Harknessii ◽  
Rust Susceptibility

Lodgepole (Pinus contorta Dougl. ex Lound.) and jack (Pinus banksiana Lamb.) pines occur sympatrically and hybridize in central and northwestern Alberta, providing opportunities for studying unique ecological and evolutionary interactions. We conducted a greenhouse inoculation experiment to investigate interactions between 40 populations of lodgepole and jack pines and their putative hybrids across this hybrid zone and two sources of the western gall rust fungus, Endocronartium harknessii (J.P. Moore) Y. Hiratsuka, one from lodgepole pine and the other from jack pine. Rust susceptibility and height were assessed when the seedlings were 6 months and 1 year old. Lodgepole pine and the hybrids were significantly more susceptible to the rust infection than jack pine. Jack pine grew significantly faster than the hybrids and lodgepole pine. In addition, the seedlings infected with spores from lodgepole pine grew significantly slower than those with spores from jack pine. While the overall rust scores indicated that spores from lodgepole pine was more virulent to the hosts than those from jack pine, both spore sources were better adapted to their own host species, causing significant spore source × host group interactions. However, such host specificity in the western gall rust is far from stabilized (equilibrium) because of continued gene exchanges among the two parental species and their hybrids.Key words: Pinus contorta - Pinus banksiana complex, western gall rust, natural hybridization, coevolutionary genetics.

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