Genomic in situ hybridization (GISH) discriminates between the A and the B genomes in diploid and tetraploid Setaria species

Genome ◽  
2001 ◽  
Vol 44 (4) ◽  
pp. 685-690 ◽  
Author(s):  
A Benabdelmouna ◽  
Y Shi ◽  
M Abirached-Darmency ◽  
H Darmency
Keyword(s):  
In Situ Hybridization ◽  
Foxtail Millet ◽  
Diploid Species ◽  
Genomic In Situ Hybridization ◽  
The Other ◽  
Polyploid Species ◽  
Nucleolar Organizing Regions ◽  
Genomic Relationships ◽  
Clear Differentiation

Genomic in situ hybridization (GISH) was used to investigate genomic relationships between different Setaria species of the foxtail millet gene pool (S. italica) and one interspecific F1 hybrid. The GISH patterns obtained on the two diploid species S. viridis (genome A) and S. adhaerans (genome B), and on their F1 hybrid showed clear differentiation between these two genomes except at the nucleolar organizing regions. Similar GISH patterns allowed differentiation of S. italica from S. adhaerans. However, GISH patterns did not distinguish between the genomes of S. italica and its putative wild ancestor S. viridis. GISH was also applied to polyploid Setaria species and enabled confirmation of the assumed allotetraploid nature of S. faberii and demonstration that both S. verticillata and S. verticillata var. ambigua were also allotetraploids. All these tetraploid species contained two sets of 18 chromosomes each, one from genome A and the other from genome B. Only one polyploid species, S. pumila, was shown to bear an unknown genomic composition that is not closely related either to genome A or to genome B.Key words: Setaria, genomic in situ hybridization, genome analysis.

Genomic relationships among diploid and polyploid species of the genus Eryngium L. using genomic in situ hybridization

Genome ◽  
2010 ◽  
Vol 53 (10) ◽  
pp. 824-831 ◽  
Author(s):  
Gisèle Yvonne Perthuy ◽  
Susana Martínez ◽  
Eduardo José Greizerstein ◽  
Lidia Poggio
Keyword(s):  
In Situ Hybridization ◽  
Morphological Variability ◽  
Diploid Species ◽  
Genomic In Situ Hybridization ◽  
Hybrid Origin ◽  
Polyploid Species ◽  
Ploidy Levels ◽  
Close Relationship ◽  
Large Genus

Eryngium L. (Umbelliferae) is a large genus including more than 250 species worldwide. The large morphological variability in this genus makes it difficult to delimit the species or to establish phylogenetic relationships. The occurrence of different ploidy levels within the genus might indicate a hybrid origin of the polyploid species. In the present study, the chromosome number and karyotype of E. regnellii are reported for the first time and the ploidy level of a population of E. paniculatum is confirmed. We compare the genomes of the diploids E. horridum and E. eburneum , the tetraploids E. megapotamicum and E. regnellii , and the hexaploids E. pandanifolium (as a representative of the whole pandanifolium complex) and E. paniculatum using genomic in situ hybridization (GISH). Although it was not possible to identify the parental species of the polyploid taxa analyzed, the GISH technique allowed us to postulate some hypotheses about their origin. Eryngium horridum and E. eburneum do not seem to be the direct progenitors of the polyploids analyzed. On the other hand, it seems that other diploid species unrelated to E. horridum and E. eburneum are involved in their origin. Our results are consistent with morphological and phylogenetic studies, indicating a close relationship between the species of the series Latifolia.

Genomic relationships among diploid and hexaploid species of Andropogon (Poaceae)

Genome ◽  
2004 ◽  
Vol 47 (6) ◽  
pp. 1220-1224 ◽  
Author(s):  
G Norrmann ◽  
L Hanson ◽  
S Renvoize ◽  
I J Leitch
Keyword(s):  
In Situ Hybridization ◽  
Diploid Species ◽  
Genomic In Situ Hybridization ◽  
South American ◽  
The North ◽  
Hexaploid Species ◽  
Genomic Relationships ◽  
Strong Hybridization ◽  
Taxonomic Implications

Andropogon is a pantropical grass genus comprising 100–120 species and found mainly in the grasslands of Africa and the Americas. While the genomic relationships between many Andropogon species have been resolved by studying chromosome behavior in interspecific hybrids, relationships between the North and South American diploids have remained elusive. Further, the genome composition of two hexaploid species (including the important forage grass Andropogon lateralis Nees) has been unclear because of the strong hybridization barriers that exist between species. Consequently, genomic in situ hybridization was applied to shed light on these issues. The results confirmed that (i) both the South American (Andropogon selloanus (Hack.) Hack., Andropogon macrothrix Trin.) and North American (Andropogon gyrans Michx.) diploid species shared a common S genome and (ii) the S genome comprises just one of the three genomes in the hexaploids A. lateralis Nees and Andropogon bicornis L. The evolutionary and taxonomic implications of these findings are discussed.Key words: Andropogon, polyploidy evolution, Poaceae, genomic in situ hybridization, taxonomy.

The genomic identification of some monosomics of Avena sativa L. cv. Sun II using genomic in situ hybridization

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 747-751 ◽  
Author(s):  
J. M. Leggett ◽  
G. S. Markhand
Keyword(s):  
In Situ Hybridization ◽  
Avena Sativa ◽  
Genomic Dna ◽  
Diploid Species ◽  
Genomic In Situ Hybridization ◽  
Chromosome Translocations ◽  
C Genome

Genomic in situ hybridization using total genomic DNA extracted from the C genome diploid species Avena eriantha (2n = 2x = 14, genome CpCp) was used to identify monosomics (2n = 6x − 1 = 41) of the constituent genomes of the hexaploid cultivated oat A. sativa L. cv. Sun II (2n = 6x = 42, genomes AACCDD). The results demonstrate 3 AD/C and 6 C/AD chromosome translocations, indicate that five of the missing monosomics are derived from the C genome, and show that there are duplicates within the partial monosomic series. Chromosome polymorphisms between some monosomic lines are also demonstrated.Key words: Avena, monosomics, genomic in situ hybridization, genomic identification.

Identification of the parental chromosomes of the wheat–alien amphiploid Agrotana by genomic in situ hybridization

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1163-1169 ◽  
Author(s):  
Qin Chen ◽  
R. L. Conner ◽  
A. Laroche
Keyword(s):  
In Situ Hybridization ◽  
Genomic Dna ◽  
Molecular Cytogenetics ◽  
Unknown Origin ◽  
Genomic In Situ Hybridization ◽  
Haynaldia Villosa ◽  
Genomic Relationships ◽  
High Degree ◽  
Donor Species

Labelled total genomic DNA from four alien species, Thinopyrum ponticum (Host) Beauv. (2n = 70, genomes J1J1J1J2J2), Th. bessarabicum (Savul. &Rayss) Love (2n = 14, genome J), Th. elongatum (Host) Beauv. (2n = 14, genome E), and Haynaldia villosa (L.) Schur. (2n = 14, genome V), were used as probes in combination with blocking wheat DNA for in situ hybridization of the chromosomes of Agrotana, a wheat–alien hybrid (2n = 56) of unknown origin. The results showed that genomic DNA probes from Th. ponticum and Th. bessarabicum both clearly revealed 16 alien and 40 wheat chromosomes in Agrotana, indicating that the J genome present in these two species has a high degree of homology with the alien chromosomes in Agrotana. Biotinylated genomic DNA probe from Th. elongatum identified 10 chromosomes from Agrotana, while some regions of six other chromosomes yielded a weak or no signal. The probe from H. villosa produced no differential labelling of the chromosomes of Agrotana. The genomic formula of Agrotana was designated as AABBDDJJ. We suggest that the alien parent donor species of Agrotana is Th. ponticum rather than Th. bessarabicum. Genomic relationships of the three Thinopyrum species are discussed in relation to the distribution of GISH signals in the chromosomes of Agrotana.Key words: Thinopyrum species, wheat–alien amphiploid, genomic DNA probing, in situ hybridization, molecular cytogenetics.

Homoeologous recombination in 2n-gametes producing interspecific hybrids of Lilium (Liliaceae) studied by genomic in situ hybridization (GISH)

Genome ◽  
1999 ◽  
Vol 42 (4) ◽  
pp. 681-686 ◽  
Author(s):  
G I Karlov ◽  
L I Khrustaleva ◽  
K B Lim ◽  
J M van Tuyl
Keyword(s):  
In Situ Hybridization ◽  
Interspecific Hybridization ◽  
2N Gametes ◽  
Lilium Longiflorum ◽  
Genomic In Situ Hybridization ◽  
2N Pollen ◽  
Rdna Sites ◽  
Clear Differentiation ◽  
Pollen Formation

Interspecific hybridization of Lilium longiflorum (L) with Asiatic (A) lily hybrids results in so-called LA-hybrids. Some of these hybrids produce 2n-pollen, which were used to perform crosses on Asiatic and Oriental (O) hybrids, resulting in ALA- and OLA-hybrids. Recombination between homoeologous chromosomes (introgression) and the mechanism of 2n-pollen formation in these hybrids were studied using genomic in situ hybridization (GISH). A clear differentiation between the chromosomes of L. longiflorum, Asiatic, and Oriental hybrids was observed in four ALA- and one OLA-hybrid using GISH. Two ALA-hybrids showed 3 and 5 recombinant chromosomes with a total of 5 and 10 crossover sites per hybrid, respectively. These occurred at random positions on the chromosomes. The number and the location of the rDNA-sites were determined using in situ hybridization and provided a tool, the FISH-marker, for identifying the NOR-bearing chromosomes in the lily hybrids. Evidence for the occurrence of the FDR-mechanism (first division restitution) of 2n-pollen formation in the LA-hybrids was obtained on the basis of absence of homologous chromosomes of L. longiflorum in the ALA- and OLA-hybrids.Key words: Lilium longiflorum, introgression, FDR, interspecific hybridization, FISH.

Characterization of wheat-Thinopyrum partial amphiploids by meiotic analysis and genomic in situ hybridization

Genome ◽  
2000 ◽  
Vol 43 (4) ◽  
pp. 712-719 ◽  
Author(s):  
George Fedak ◽  
Qin Chen ◽  
Robert L Conner ◽  
André Laroche ◽  
René Petroski ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Genomic In Situ Hybridization ◽  
The Other ◽  
Meiotic Pairing ◽  
Genomic Constitution ◽  
Meiotic Analysis ◽  
Partial Amphiploid ◽  
Partial Amphiploids

A combination of genomic in situ hybridization (GISH) and meiotic pairing analysis of wheat-Thinopyrum partial amphiploids was employed to identify the genomic constitution and relationships between partial amphiploids derived from wheat and wheatgrass crosses. On the basis of similarities in the meiotic behavior and GISH patterns, the alien chromosomes of two of eight partial amphiploids, TAF46 and 'Otrastayuskaya 38', were judged to originate from Th. intermedium, whereas Th. ponticum was one of the parents of the other six partial amphiploids; PWM706, PWM206, PWM209, PWMIII, OK7211542, and Ag-wheat hybrid. Each of these partial amphiploids was found to contain a synthetic alien genome composed of different combinations of St-, J-, or Js-genome chromosomes. For relatedness of partial amphiploid lines, meiotic analysis of F1 hybrids and GISH results were generally complementary, but the latter offered greater precision in identifying constituent genomes.Key words: wheat, Thinopyrum, partial amphiploids, GISH, meiotic analysis.

In situ hybridization analysis indicates that 4AL–5AL–7BS translocation preceded subspecies differentiation of Triticum turgidum

Genome ◽  
2013 ◽  
Vol 56 (5) ◽  
pp. 303-305 ◽  
Author(s):  
Ming Hao ◽  
Jiangtao Luo ◽  
Lianquan Zhang ◽  
Zhongwei Yuan ◽  
Youliang Zheng ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Triticum Turgidum ◽  
Genomic In Situ Hybridization ◽  
Hybridization Analysis ◽  
The Other ◽  
Subspecies Differentiation

The important cyclic translocation 4AL–5AL–7BS is an evolutionary signature of polyploidy in wheat. This study aimed to determine its distribution within the subspecies of Triticum turgidum L., using genomic in situ hybridization and fluorescence in situ hybridization. As it exists in all eight subspecies, this translocation appeared before the differentiation of the subspecies of T. turgidum. This translocation probably first appeared in T. turgidum subsp. dicoccoides and was then transmitted into the other subspecies. Its existence in all of the analyzed subspecies suggests that this translocation may confer an adaptive advantage during the course of evolution.

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