Sensitivity of Estimates of Seasonal Phytoplankton Photosynthesis to Sampling Frequency
Phytoplankton photosynthesis was monitored once or twice weekly in an oligotrophic Canadian Shield lake in northwestern Ontario during the ice-free season for 6 yr. These data sets were subsampled in various ways to determine how the precision of estimates of ice-free season phytoplankton photosynthesis was affected by sampling frequency. Deviations were as large as 20% if a measurement was not taken before thermal stratification was established in the spring, or if samples were taken at monthly intervals during the ice-free season. Shorter sampling intervals (1, 2, and 3 wk were tested) all resulted in deviations of about 10%. Taking samples irregularly (every day for the 3 d subsequent to rainfall events ≥ 5 mm) instead of at regular time intervals did not reduce deviations.