Estimation of Algal Succession in Lake Water Using HPLC Analysis of Pigments

1993 ◽  
Vol 50 (6) ◽  
pp. 1142-1146 ◽  
Author(s):  
Yuko Soma ◽  
Takashi Imaizumi ◽  
Kei-ichi Yagi ◽  
Sei-ichi Kasuga
Keyword(s):  
Seasonal Variation ◽  
Chlorophyll A ◽  
Green Algae ◽  
Lake Water ◽  
Algal Biomass ◽  
Hplc Analysis ◽  
Total Chlorophyll ◽  
Pigment Analysis ◽  
Algal Succession ◽  
Chlorophyll A Content

Seasonal variation in algal biomass in lake water was estimated using HPLC analysis of pigments. Carotenoids/chlorophyll a ratios were determined for cultures of Anabena, Microcystis, green algae, diatoms, and Cryptomonas. The contributions of various algal taxa to the total chlorophyll a content of lake water were calculated using the average carotenoid/chlorophyll a ratios of fingerprint pigments. The pigment analysis reflected the observed trend in the numbers of algae in lake water and proved to be a useful supplementary approach to evaluate algal biomass.

Are seasonal lakes as productive as permanent lakes? A case study from Ireland

2010 ◽  
Vol 67 (8) ◽  
pp. 1291-1302 ◽  
Author(s):  
Helder Cunha Pereira ◽  
Norman Allott ◽  
Catherine Coxon
Keyword(s):  
Seasonal Variation ◽  
Linear Regression ◽  
Chlorophyll A ◽  
Algal Biomass ◽  
P Limitation ◽  
Underlying Causes ◽  
Western Ireland ◽  
Log Linear ◽  
First Time

This paper compares, for the first time, nutrient levels and chlorophyll a measured in a set of seasonal lakes with those reported for permanent lakes in the literature. Twenty-two turloughs (karstic seasonal lakes) in western Ireland were sampled monthly from the onset of flooding (October) until they dried out (6 to 9 months). The turloughs showed similar levels of nutrients and chlorophyll a to those reported for Irish and international lakes. Chlorophyll a peaked between November and February in the majority of turloughs, sometimes with values higher than those measured in mesotrophic lakes in summer. A significant log-linear regression was found between total phosphorus and chlorophyll a, which suggests P limitation of algal biomass in the majority of the turloughs. The regression characteristics were not significantly different than those described in similar studies of permanent lakes. Patterns in seasonal variation of nutrients are also presented, their underlying causes being discussed in relation to their transport within catchments. Our results show that despite being predominantly winter phenomena, turloughs can be as productive as permanent lakes.

Improving in-lake water quality modeling using variable chlorophyll a/algal biomass ratios

2018 ◽  
Vol 101 ◽  
pp. 73-85 ◽  
Author(s):  
Amir Sadeghian ◽  
Steven C. Chapra ◽  
Jeff Hudson ◽  
Howard Wheater ◽  
Karl-Erich Lindenschmidt
Keyword(s):  
Water Quality ◽  
Chlorophyll A ◽  
Lake Water ◽  
Algal Biomass ◽  
Water Quality Modeling ◽  
Lake Water Quality ◽  
Quality Modeling

scholarly journals Mediterranean rivers: Consequences of water scarcity on benthic algal chlorophyll a content

2016 ◽  
Vol 76 (s1) ◽  
Author(s):  
Elena Piano ◽  
Elisa Falasco ◽  
Francesca Bona
Keyword(s):  
Chlorophyll A ◽  
Green Algae ◽  
Water Scarcity ◽  
Trophic Chain ◽  
Environmental Predictors ◽  
Mediterranean Rivers ◽  
Drought Period ◽  
Ligurian Alps ◽  
Algal Groups ◽  
Chlorophyll A Content

Mediterranean rivers are subjected to strong seasonality with drought during the hot season and extreme flows in autumn-winter. In particular, drought episodes and water scarcity alter the river morphology, with repercussions on primary production and the trophic chain. In this paper, we aimed at analysing the different responses in terms of chlorophyll a content of the three main photosynthetic groups composing stream periphyton, namely diatoms, cyanobacteria and green algae. This work was conducted in the Ligurian Alps (NW-Italy) on five oligotrophic streams (Argentina, Impero, Merula, Quiliano and Vallecrosia), similar in terms of physico-chemical parameters. We measured chlorophyll a content of diatoms, cyanobacteria and green algae by means of an in situ fluorimetric probe (BenthoTorch®). Data were collected from April to October 2014 in: i) impacted sites, where the water scarcity was exacerbated by human pressure; ii) control sites. We applied Generalized Linear Mixed Models to investigate the response of total chlorophyll a and its relative proportions among the three algal groups in relation to the following environmental predictors: water depth, flow velocity, canopy shading, microhabitat isolation, sampling season, dissolved oxygen, temperature, pH, nutrients, and macrophyte coverage.Results showed an opposite response of diatoms and green algae. Diatoms were favoured in the control sites and under moderate flow conditions, while the probability of green algae presence was higher in the impacted sites and during the drought season. Cyanobacteria showed a response similar to green algae, preferring warm, isolated pools typical of the drought period. Diatoms proved to be the most sensitive to drought. More specifically, we found out that percentages of diatoms below 51% with respect to total benthic chlorophyll a indicate high hydrological disturbance. This study provides the first evidence that the proportion of chlorophyll a produced by diatoms can be a suitable indicator for monitoring programs aiming at determining the effects of water scarcity on river ecosystems.

scholarly journals Risk Assessment of Urban Lake Water Quality Based on in-situ Cyanobacterial and Total Chlorophyll-a Monitoring

2016 ◽  
Vol 25 (2) ◽  
pp. 655-661 ◽  
Author(s):  
Hazem Kalaji ◽  
Oksana Sytar ◽  
Marian Brestic ◽  
Izabela Samborska ◽  
Magdalena Cetner ◽  
...  
Keyword(s):  
Water Quality ◽  
Risk Assessment ◽  
Chlorophyll A ◽  
Lake Water ◽  
Total Chlorophyll ◽  
Urban Lake ◽  
Lake Water Quality

scholarly journals Seasonal Variation of Chlorophyll and Carotenoids in Leaves of Hancornia speciosa in Three Central Areas of the Cerrado of the State of Tocantins, Brazil

2018 ◽  
Vol 10 (3) ◽  
pp. 344
Author(s):  
Rafael J. de Oliveira ◽  
José Expedito C. da Silva ◽  
Davi B. das Chagas
Keyword(s):  
Seasonal Variation ◽  
Chlorophyll A ◽  
Temporal Analysis ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Dry Period ◽  
Rainy Period ◽  
Significant Difference ◽  
Spatio Temporal ◽  
Hancornia Speciosa

This study aimed to evaluate the seasonal variation in concentrations of chlorophyll a, b, total chlorophyll and carotenoids in leaves of Hancornia speciosa, Gomes, during the periods of the year, relating them to the main phenological events, periods (rainy, dry and transitions) and populations evaluated. The survey was performed in three sites and the spatio-temporal analysis divided into four periods (rainy, rainy-drytransition, dry and dry-rainy transition), with 10 replicates (matrix plants). The data were collected in average intervals of 33 days from October 2014 to April 2017. The extraction and calculation of the chlorophyll and carotenoid contents of the leaves were expressed in mg/g DM, according to the equations of Arnon (1949) and Lichtenthaler (1987). There was a significant difference between the periods and sites analyzed for all pigments and their relationships. There was a greater amount of chlorophyll a than chlorophyll b; this difference was greater in the dry period. In the rainy period, we found a greater amount of total chlorophyll, carotenoids and total chlorophyll/carotenoid ratio. The behavior for the species follows that already observed for deciduous plants, closely related to water availability.

Distribution of epipelic algae and related environmental nutrients in Taihu Lake, revealed by HPLC analysis

2019 ◽  
Vol 70 (6) ◽  
pp. 849
Author(s):  
Yuzhi Song ◽  
Yan Xue ◽  
Yongxia Gao ◽  
Yan Jin
Keyword(s):  
Green Algae ◽  
Algal Biomass ◽  
Taihu Lake ◽  
Lake Taihu ◽  
Eastern China ◽  
Sampling Site ◽  
Algal Community ◽  
Pigment Analysis ◽  
Chl A ◽  
Meiliang Bay

Epipelic algal biomass and composition (determined by HPLC pigment analysis) and related environmental nutrients were investigated in a phytoplankton-dominated area (Meiliang Bay), a macrophyte-dominated area (East Taihu Lake) and a transition zones (Gonghu Bay) in a shallow eutrophic freshwater lake (Taihu Lake, eastern China). Surface sediment samples were collected at 11 sampling sites in April and August 2016. Average epipelic algal biomass for Meiliang Bay, Gonghu Bay and East Taihu Lake was 6.5, 4.9 and 4.6μg chlorophyll (Chl)-ag–1 sediment respectively in April, and 3.8, 7.4 and 2.7μg Chl-ag–1 sediment respectively in August. The maximum biomass of epipelic algae appeared at the G1 sampling site (9.6μg Chl-ag–1 sediment) in August, whereas the minimum biomass of epipelic algae appeared at the E4 sampling site (1.7μg Chl-ag–1 sediment). Pigment (fucoxanthin, zeaxanthin and Chl-b) to Chl-a ratios indicated that epipelic algae were primarily diatoms, secondarily cyanobacteria and lastly green algae in Taihu Lake. Epipelic algae were significantly correlated with total nitrogen in the water and total phosphorus in the sediment (P<0.05), except for green algae. In general, using pigment as an indicator revealed that the epiphytic algal community varied over time and space. The distribution of variations in epipelic algae was related to the heterogeneity of environmental factors.

Effects of Phosphorus Stress on Photosynthetic Pigments in Sagittaria sagittifolia

2014 ◽  
Vol 1073-1076 ◽  
pp. 118-121
Author(s):  
Qing Qing He ◽  
Zhou Li Liu ◽  
Wei Chen ◽  
Lin Hao ◽  
Jiao Jiao Qin ◽  
...  
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Carotenoid Content ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Phosphorus Stress ◽  
Sagittaria Sagittifolia ◽  
Chlorophyll A Content

The photosynthetic pigments of Sagittaria sagittifolia, including total chlorophyll content, chlorophyll a content, chlorophyll b content and carotenoid content, were investigated after 0, 7, 14 and 21 d exposure to 3 mg L-1 P. After 7 d P-exposure, total chlorophyll content, chlorophyll a content and chlorophyll b content increased significantly compared with the control, and then decreased with time extended. Carotenoid content increased significantly after 14 d P-exposure compared with the control, and then decreased slightly after 21 d P-exposure.

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