Influence of light on chlorophyll, a content of blue-green algae treated with heavy metals

1987 ◽  
Vol 38 (6) ◽  
pp. 1062-1069 ◽  
Author(s):  
P. A. Azeez ◽  
D. K. Banerjee
Keyword(s):  
Heavy Metals ◽  
Chlorophyll A ◽  
Green Algae ◽  
Blue Green Algae ◽  
Chlorophyll A Content

scholarly journals Research of pigments of blue-green algae spirulina platensis for practical use in confectionery technology

2019 ◽  
Vol 81 (2) ◽  
pp. 170-176
Author(s):  
T. K. Kalenik ◽  
E. V. Dobrynina ◽  
V. M. Ostapenko ◽  
Y. Torii ◽  
J. Hiromi
Keyword(s):  
Chlorophyll A ◽  
Green Algae ◽  
Spirulina Platensis ◽  
Food Additives ◽  
Water Extract ◽  
Blue Pigment ◽  
Natural Food ◽  
Synthetic Dyes ◽  
Blue Green Algae ◽  
Synthetic Origin

The article presents a study of the process of isolation of natural blue pigment – phycocyanin from the biomass of blue-green algae Spirulina platensis by water extraction, followed using its water solution as a natural food colorant in the production of milk chocolate. Recently, modern food enterprises are pursuing their policy towards expanding the range of products, which is closely related to the increasing needs of the population in food of a new kind. One of the solutions to this problem is the use of food additives of both natural and synthetic origin. Among the similar components widespread found dyes synthetic origin, which have high coverage rates and relatively low cost. However, many of the permitted in our country synthetic food dyes are banned in several developed countries as potentially dangerous to health. Synthetic dyes of red, yellow and green color have many natural analogues – carotenoids, lutein, chlorophyll, etc., except for the blue dye, the analogue of which is only anthocyanins, which are unstable depending on the pH conditions. In this article were identified phycobiliproteins and chlorophyll a in a water extract of spirulina. The mass concentration of phycobiliproteins and chlorophyll a was determined by spectrophotometric method before and after the addition of ammonium sulfate. A comparative analysis of the effect of fractionation (salting out) on the degree of purification of the phycocyanin solution. Presented and described the technological scheme of extraction of phycocyanin which allows to use it in food technologies as an extract or a dry powder. Established the concentration of phycocyanin extract from blue-green algae spirulina to produce milk blue chocolate. Determined organoleptic and hygienic characteristics of the finished product

Comparison of Chlorophyll a Extractions with Ethanol and Dimethyl Sulfoxide/Acetone, and a Concern about Spectrophotometric Phaeopigment Correction

1992 ◽  
Vol 49 (11) ◽  
pp. 2331-2336 ◽  
Author(s):  
D. J. Webb ◽  
B. K. Burnison ◽  
A. M. Trimbee ◽  
E. E. Prepas
Keyword(s):  
High Pressure ◽  
Dimethyl Sulfoxide ◽  
Chlorophyll A ◽  
Green Algae ◽  
Natural Populations ◽  
Eutrophic Lakes ◽  
North Central ◽  
Chl A ◽  
Blue Green Algae ◽  
Hplc Analyses

Chlorophyll a (Chl a) in water samples from three mesotrophic to eutrophic lakes in north-central Alberta was extracted with one of three solvents (95% ethanol, 90% ethanol, or a 2:3 mixture of dimethyl sulfoxide and 90% acetone (DMSO/acetone)) and analyzed by two techniques (spectrophotometry and high pressure liquid chromatography (HPLC). The dominant phytoplankton were blue-green algae and diatoms. Total Chl a concentrations (i.e. no correction for phaeopigments (Pha)) were not significantly different among solvents (P > 0.5). Total Chl a concentrations from spectrophotometric analyses were significantly higher than those from HPLC analyses (4.2 ± 0.88 and 2.6 ± 0.50 μg∙L−1 respectively, P < 0.05). Pha concentrations derived by spectrophotometry were 64 times higher than those derived by HPLC (1.7 ± 0.52 and 0.025 ± 0.01 μg∙L−1 respectively, P < 0.005). Thus, spectrophotometry appears to dramatically overestimate Pha concentrations and may overestimate total Chl a (i.e. no correction for Pha). Therefore, ethanol and DMSO/acetone are equally suitable for Chl a extraction from natural populations dominated by blue-green algae and/or diatoms, but if information on Pha and/or accessory pigments is required, HPLC analyses are the appropriate route rather than spectrophotometry.

scholarly journals Economically Potential Pigments from Marine Blue-Green Algae for the Application in Food and Health

2019 ◽  
Vol 1 (2) ◽  
pp. 37
Author(s):  
Katarina Purnomo Salim ◽  
Rosita Dwi Chandra ◽  
Heriyanto Heriyanto ◽  
Dwi Susilaningsih ◽  
Leenawaty Limantara ◽  
...  
Keyword(s):  
Economic Development ◽  
Chlorophyll A ◽  
Green Algae ◽  
Industrial Sector ◽  
Marine Resources ◽  
Marine Cyanobacteria ◽  
Blue Green Algae ◽  
Β Carotene

Current efforts to explore the potency of marine resources have been made to promote economic development in Indonesia, especially in the industrial sector. Marine cyanobacteria such as Arthospira sp. and Scytonema sp. are two potential candidates of the blue-green algae that can be used in the application for food and health industries. This study focused on the identification of the composition of the dominant chlorophylls and carotenoids. The results showed the presence of Zeaxanthin, Chlorophyll a, and β-carotene. The economic potency of those dominant pigments will be reviewed in this article.

scholarly journals Mediterranean rivers: Consequences of water scarcity on benthic algal chlorophyll a content

2016 ◽  
Vol 76 (s1) ◽  
Author(s):  
Elena Piano ◽  
Elisa Falasco ◽  
Francesca Bona
Keyword(s):  
Chlorophyll A ◽  
Green Algae ◽  
Water Scarcity ◽  
Trophic Chain ◽  
Environmental Predictors ◽  
Mediterranean Rivers ◽  
Drought Period ◽  
Ligurian Alps ◽  
Algal Groups ◽  
Chlorophyll A Content

Mediterranean rivers are subjected to strong seasonality with drought during the hot season and extreme flows in autumn-winter. In particular, drought episodes and water scarcity alter the river morphology, with repercussions on primary production and the trophic chain. In this paper, we aimed at analysing the different responses in terms of chlorophyll a content of the three main photosynthetic groups composing stream periphyton, namely diatoms, cyanobacteria and green algae. This work was conducted in the Ligurian Alps (NW-Italy) on five oligotrophic streams (Argentina, Impero, Merula, Quiliano and Vallecrosia), similar in terms of physico-chemical parameters. We measured chlorophyll a content of diatoms, cyanobacteria and green algae by means of an in situ fluorimetric probe (BenthoTorch®). Data were collected from April to October 2014 in: i) impacted sites, where the water scarcity was exacerbated by human pressure; ii) control sites. We applied Generalized Linear Mixed Models to investigate the response of total chlorophyll a and its relative proportions among the three algal groups in relation to the following environmental predictors: water depth, flow velocity, canopy shading, microhabitat isolation, sampling season, dissolved oxygen, temperature, pH, nutrients, and macrophyte coverage.Results showed an opposite response of diatoms and green algae. Diatoms were favoured in the control sites and under moderate flow conditions, while the probability of green algae presence was higher in the impacted sites and during the drought season. Cyanobacteria showed a response similar to green algae, preferring warm, isolated pools typical of the drought period. Diatoms proved to be the most sensitive to drought. More specifically, we found out that percentages of diatoms below 51% with respect to total benthic chlorophyll a indicate high hydrological disturbance. This study provides the first evidence that the proportion of chlorophyll a produced by diatoms can be a suitable indicator for monitoring programs aiming at determining the effects of water scarcity on river ecosystems.

Estimation of Algal Succession in Lake Water Using HPLC Analysis of Pigments

1993 ◽  
Vol 50 (6) ◽  
pp. 1142-1146 ◽  
Author(s):  
Yuko Soma ◽  
Takashi Imaizumi ◽  
Kei-ichi Yagi ◽  
Sei-ichi Kasuga
Keyword(s):  
Seasonal Variation ◽  
Chlorophyll A ◽  
Green Algae ◽  
Lake Water ◽  
Algal Biomass ◽  
Hplc Analysis ◽  
Total Chlorophyll ◽  
Pigment Analysis ◽  
Algal Succession ◽  
Chlorophyll A Content

Seasonal variation in algal biomass in lake water was estimated using HPLC analysis of pigments. Carotenoids/chlorophyll a ratios were determined for cultures of Anabena, Microcystis, green algae, diatoms, and Cryptomonas. The contributions of various algal taxa to the total chlorophyll a content of lake water were calculated using the average carotenoid/chlorophyll a ratios of fingerprint pigments. The pigment analysis reflected the observed trend in the numbers of algae in lake water and proved to be a useful supplementary approach to evaluate algal biomass.

Comparison of N,N-Dimethylformamide, Dimethyl Sulfoxide, and Acetone for Extraction of Phytoplankton Chlorophyll

1984 ◽  
Vol 41 (10) ◽  
pp. 1519-1522 ◽  
Author(s):  
Barbara J. Speziale ◽  
Stephen P. Schreiner ◽  
Paul A. Giammatteo ◽  
James E. Schindler
Keyword(s):  
Dimethyl Sulfoxide ◽  
Chlorophyll A ◽  
Green Algae ◽  
Absorption Coefficients ◽  
Extinction Coefficients ◽  
Blue Green Algae ◽  
Incomplete Mixing ◽  
The Difference ◽  
Absorbance Spectra ◽  
Algal Cultures

We evaluated the suitability of N,N-dimethylformamide (DMF) for extraction and measurement of chlorophyll in phytoplankton and compared the extraction efficiencies of DMF, dimethyl sulfoxide (DMSO)–acetone, and 90% acetone. Absorbance spectra and specific absorption coefficients of chlorophylls a, b, and c in DMF and 90% acetone are similar. Acidification of 100% DMF extracts of pure chlorophyll a and healthy algal cultures produced acid ratios approximating 1.7. Thus, equations derived from the extinction coefficients of chlorophylls in 90% acetone can be applied to DMF extracts. DMF and DMSO–acetone each extract chlorophyll from green and blue-green algae more effectively than does 90% acetone. DMF is a better extractant than is DMSO–acetone for chlorococcalean species, especially when pigment concentrations are high, but the difference, although significant, is slight. The three solvents are equally efficient in extracting chlorophyll from samples composed of chrysophytes and flagellated chlorophytes. The lack of grinding when using either DMF or DMSO–acetone is a considerable convenience. The potential for destabilized spectrophotometer readings and contamination of the investigator due to the incomplete mixing of DMSO and acetone is eliminated when pure DMF is used.

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