Survival of Two Bacterial Fish Pathogens (Aeromonas salmonicida and the Enteric Redmouth Bacterium) in Ozonated, Chlorinated, and Untreated Waters

1977 ◽  
Vol 34 (3) ◽  
pp. 429-432 ◽  
Author(s):  
Gary A. Wedemeyer ◽  
Nancy C. Nelson

Ozone and chlorine inactivation curves were determined in three water types at 20 °C for the destruction of the fish pathogens Aeromonas salmonicida, the etiologic agent of furunculosis, and the enteric redmouth bacterium (ERM). In phosphate-buffered distilled water, 0.01 mg/ℓ ozone inactivated 103 cells/ml of ERM and A. salmonicida in 1/2 and 10 min, respectively. Chlorine at this concentration had little effect on either pathogen and a residual of at least 0.05 mg/ℓ was needed to achieve a complete kill within a 10-min contact time. In soft lake water (30 mg/ℓ as CaCO3) a chlorine residual of 0.1 mg/ℓ rapidly [Formula: see text] inactivated A. salmonicida and ERM but in hard water (120 mg/ℓ) A. salmonicida was more resistant and 0.2 mg/ℓ chlorine was required. Ozonation of the two lake waters at 90 mg O3∙h−1∙ℓ−1 (equivalent to a 0.01 mg/ℓ residual in ozone demand-free water) was required to destroy both pathogens within 10 min.In untreated soft lake water 103 cells/ml of A. salmonicida survived only 2 days, while the ERM bacterium (103 cells/ml) survived even after 20 day s in soft and hard untreated lake waters.

1978 ◽  
Vol 35 (6) ◽  
pp. 875-879 ◽  
Author(s):  
Gary A. Wedemeyer ◽  
Nancy C. Nelson ◽  
Cathy A. Smith

Ozone and chlorine inactivation curves were determined in three water types at 10 °C for the fish pathogenic viruses infectious hematopoietic necrosis (IHNV) and infectious pancreatic necrosis (IPNV). In phosphate-buffered, distilled water (PBDW) an ozone dose of 0.01 mg/L for 30 or 60 s inactivated IHNV or IPNV, respectively, suspended at a tissue culture 50% infective dose (TCID50) of 104–105/mL. In hard (120 mg/L as CaCO3) and soft water (30 mg/L) lake waters, an ozone application rate of 70 mg∙h−1∙L−1 for 10 min destroyed IHNV. IPNV inactivation in hard water required 90 mg∙O3∙h−1∙L−1 for 10 min but only a 30-s contact time in soft water. The IPNV was also somewhat more resistant to chlorine. In PBDW, a residual of 0.1 mg/L with contact times of 30 and 60 s, respectively, destroyed IHNV and IPNV. In soft lake water IHNV was destroyed within 5 min at 0.5 mg/L, while in hard water a 10-min contact time was required. For IPNV disinfection in soft water, 0.2 mg/L for 10 min was sufficient but this chlorine residual had essentially no effect on IPNV in hard water. Increasing this dose to 0.7 mg/L destroyed IPNV in hard water within 2 min. In untreated waters, IPNV was stable for at least 8 wk in either distilled, soft, or hard lake waters. However, IHNV survived only about 2 wk in distilled and 7 wk in the soft or hard lake waters. We suggest the serious consideration of ozone as a fish disease control agent. Key words: ozone, chlorine disinfection, fish pathogens, viruses


1977 ◽  
Vol 34 (8) ◽  
pp. 1244-1249 ◽  
Author(s):  
G. L. Bullock ◽  
H. M. Stuckey

Filtration (25 nm) and ultraviolet irradiation dosages of 13,100–29,400 microwatt seconds per square centimetre (μW∙s∙cm−2) effected a 99.98–100% reduction of five gram-negative fish pathogens — Aeromonas salmonicida, A. hydrophila, Vibrio anguillarum, Pseudomonas fluorescens, and the enteric redmouth organism in 12.5 °C clear spring water or spring water containing particulate matter. Filtration and a dosage of 4500 μW∙s∙cm−2 killed 99.83–100% of test strains in spring water and 4000–4750 μW∙s∙cm−2 killed 99.33–99.99% in water with particulate matter. Irradiation of unfiltered water containing particulate matter was less effective, especially at dosages of 5000 μW∙s∙cm−2 or less, which killed 97–99.94% of strains. Filtration and 13,100 μW∙s∙cm−2 irradiation of water containing A. salmonicida prevented transmission of furunculosis. Key words: ultraviolet irradiation, bacterial fish pathogens, water disinfection


Elem Sci Anth ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Kosuke Ota ◽  
Yusuke Yokoyama ◽  
Yosuke Miyairi ◽  
Shinya Yamamoto ◽  
Toshihiro Miyajima

Lakes are sensitive recorders of anthropogenic activities, as human society often develops in their vicinity. Lake sediments thus have been widely used to reconstruct the history of environmental changes in the past, anthropogenic, or otherwise, and radiocarbon dating provides chronological control of the samples. However, specific values of radiocarbon in different carbon reservoirs due to the different pathways of radiocarbon from the upper atmosphere to the lake, called the radiocarbon reservoir age, is always difficult to evaluate because of dynamic processes in and around lakes. There are few systematic studies on radiocarbon reservoir ages for lakes owing to the complex radiocarbon transfer processes for lakes. Here, we investigate lake waters of the Fuji Five Lakes with monthly monitoring of the radiocarbon reservoir effects. Radiocarbon from dissolved inorganic carbon (DIC) for groundwater and river water is also measured, with resulting concentrations (Δ14C) at their lowest at Lake Kawaguchi in August 2018 (–122.4 ± 3.2‰), and at their highest at Lake Motosu in January 2019 (–22.4 ± 2.5‰), despite a distance of 25 km. However, winter values in both lakes show similar trends of rising Δ14C (about 20‰). Our lake water DIC Δ14C results are compared to previously published records obtained from sediments in Lake Motosu and Lake Kawaguchi. These suggest that total organic carbon and compound-specific radiocarbon found in sediments are heavily influenced by summer blooms of aquatic organisms that fix DIC in water. Thus, future studies to conduct similar analyses at the various lakes would be able to provide further insights into the carbon cycle around inland water, namely understanding the nature of radiocarbon reservoir ages.


Author(s):  
Bambang Trisakti ◽  
Nana Suwargana ◽  
I Made Parsa

Land conversion occurred in the lake catchment area caused the decreasing of water quality in many lakes of Indonesia. According to Lake Ecosystem Management Guidelines from Ministry of Environment, tropic state of lake water is one of parameters for assessing the lake ecosystem status. Tropic state can be indicated by the quantity of nitrogen, phosphorus, chlorophyll, and water clarity. The objective of this research is to develop the water quality algorithm and map the water clarity of lake water using Landsat 8 data. The data were standardized for sun geometry correction and atmospheric correction using Dark Object Subtraction method. In the first step, Total Suspended Solid (TSS) distributions in the lake were calculated using a semi empirical algorithm (Doxaran et al., 2002), which can be applied to a wide range of TSS values. Secchi Disk Transparency (SDT) distributions were calculated using our water clarity algorithm that was obtained from the relationship between TSS and SDT measured directly in the lake waters. The result shows that the water clarity algorithm developed in this research has the determination coefficient that reaches to 0,834. Implementation of the algorithm for Landsat 8 data in 2013 and 2014 showed that the water clarity in Kerinci Lake waters was around 2 m or less, but the water clarity in Tondano Lake waters was around 2 – 3 m. It means that Kerinci Lake waters had lower water clarity than Tondano Lake waters which is consistent with the field measurement results.


1992 ◽  
Vol 37 (3) ◽  
pp. 333-345 ◽  
Author(s):  
Ramon Aravena ◽  
Barry G. Warner ◽  
Glen M. MacDonald ◽  
Karen I. Hanf

AbstractCarbon-13 profiles and radiocarbon dates were obtained from two Canadian kettle basins having similar geological and hydrological characteristics to develop criteria for evaluating the validity of radiocarbon dates on lake sediment from basins in calcareous terrain. Radiocarbon dates from a site in Alberta show a variable hard-water effect related to local hydrological changes during postglacial history of the lake basin, whereas radiocarbon dates from the other site in Ontario show no noticeable influence of old carbon during its history. These differences are mainly related to lake water residence time, which has influenced carbon isotopic exchange between atmospheric CO2 and dissolved inorganic carbon in lake water. δ13C values for bulk organic sediment and terrestrial and aquatic macrofossils reveal that the main component of lake sediment at both sites is autocthonous in origin. Furthermore, each site supported different submerged aquatic plant communities that used different sources of carbon for photosynthesis, thereby imprinting the organic sediments with a characteristic 13C composition. Both sites reflect a clear relationship between 13C values and paleoproductivity. This study shows the individualistic response of the developing lake system to the hydrology, lake biota, and local geology, and demonstrates the problem of using 13C in lake sediments as a single criterion to recognize the validity of radiocarbon dates of lake sediment without supporting paleoecological information.


2021 ◽  
Vol 19 (2) ◽  
pp. 83-89
Author(s):  
Arvind Pathak ◽  
Pratima Khadka ◽  
Prabin Karki

Detergents commercially available in the Nepalese market were studied and several parameters such as surface tension, pH, critical micelle concentration, foaming stability test, hard water test, emulsions stability test were performed. Different medium such as ground water (G.W.), tap water (T.W.), distilled water (D.W.) and 5% ethanol in distilled water were selected for this study. The decrease in surface tension and critical micelle concentration (CMC) in ground water, tap water, distilled water and 5% ethanol in distilled water, ease of cleansing action of the detergents in this medium have been found of the following order: 5% ethanol in distilled water > distilled water (D.W.) > tap water (T.W.) > ground water (G.W.). Among the four detergents, the D1 have shown the least surface tension, CMC value, foam collapsing time, the weight of scum formed when treated with hard water. And maximum emulsion stability of the detergent D1 determines good quality detergent.


2011 ◽  
Vol 287-290 ◽  
pp. 1811-1814
Author(s):  
Xiao Guo Chen ◽  
Rui Ting Yan ◽  
Xiao Yan Wu ◽  
Yue Hu

The photocatalytic degradation of MCRR has been investigated using Ag-doped TiO2 films as photocatalyst and sunlight as irradiation source. Ag-doped TiO2 films can significantly accelerate the degradation of MCRR both in distilled water and in lake water, although the efficiency in lake water was not good as in distilled water. The photocatalytic activity was affected by the number of coating cycles and the best performance was found in films prepared with five coatings. Solution pH can also significantly influence the photocatalytic degradation of MCRR, and acidic pH was more efficient for the degradation of MCRR.


1978 ◽  
Vol 35 (1) ◽  
pp. 141-143 ◽  
Author(s):  
J. Barica

The Orion cupric ion electrode exhibited the theoretical (Nernstian) response of 29–30 mV per concentration decade in deionized water solutions, but in certain moderately alkaline and moderately saline natural waters of the Canadian prairies (pH 7.8–8.6, specific conductance 950–6550 μS/cm) the response to added cupric ion of over 100 μg Cu/L was twice as great. The electrode was, nevertheless, usable in natural waters provided that calibration was made with water from the same source. Key words: water analysis (chemical), cupric ion electrode, copper, hard water


Radiocarbon ◽  
2001 ◽  
Vol 43 (2B) ◽  
pp. 821-830 ◽  
Author(s):  
Mariana Stiller ◽  
Aaron Kaufman ◽  
Israel Carmi ◽  
Genia Mintz

The source of endogenic organic and inorganic carbon in lacustrine sediments is the dissolved inorganic carbon (DIC) in the lake water. The relation between the radiocarbon levels of DIC in Lake Kinneret and of CO2 in the atmosphere has been investigated. The ratio of the former to the latter was found to be 0.814 ± 0.013. This ratio is used for calibrating the age of the sediment according to the natural fluctuations in the atmospheric levels of 14C that occurred during the past 10,000 years.


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