Immune Response of Juvenile Coho Salmon (Oncorhynchus kisutch) to Aeromonas salmonicida Cells Administered Intraperitoneally in Freund's Complete Adjuvant

1974 ◽  
Vol 31 (11) ◽  
pp. 1751-1755 ◽  
Author(s):  
W. D. Paterson ◽  
J. L. Fryer
Keyword(s):  
Immune Response ◽  
Antibody Response ◽  
Protective Immunity ◽  
Coho Salmon ◽  
Oncorhynchus Kisutch ◽  
Aeromonas Salmonicida ◽  
Homogeneous Population ◽  
Freund’S Complete Adjuvant ◽  
Freund's Complete Adjuvant ◽  
Single Vaccination

The antibody response of a population of juvenile coho salmon (Oncorhynchus kisutch) held at 12 C approached 100% following a single parenteral vaccination with Aeromonas salmonicida cells emulsified in Freund's complete adjuvant. This response indicates that coho salmon behave as a homogeneous population with respect to immunological competence.Fish as small as 1.2 g produced antibodies as early as 4 wk after administration of this vaccine; high levels of antibody persisted for at least 1 yr after injection. This single vaccination provided juvenile coho salmon with protective immunity against furunculosis.

scholarly journals Evaluation of different immunization protocols with P. brasiliensis antigens in Guinea pigs

1992 ◽  
Vol 34 (3) ◽  
pp. 243-249
Author(s):  
Amélia Kamegasawa ◽  
Maria Tereza Rezkallah Iwasso ◽  
Rosa Viero ◽  
Marcello Franco
Keyword(s):  
Immune Response ◽  
Guinea Pigs ◽  
Protective Immunity ◽  
Migration Inhibition ◽  
Freund’S Complete Adjuvant ◽  
Future Studies ◽  
Freund's Complete Adjuvant ◽  
Particulate Antigen ◽  
Long Time ◽  
Macrophage Migration Inhibition

The objective of the present study was to develop an efficient and reproducible protocol of immunization of guinea pigs with P. brasiliensis antigens as an animal model for future studies of protective immunity mechanisms. We tested three different antigens (particulate, soluble and combined) and six protocols in the presence and absence of Freund's complete adjuvant and with different numbers of immunizing doses and variable lenght of time between the last immunizing dose and challenge. The efficacy of the immunizing protocol was evaluated by measuring the humoral and cellular anti-P. brasiliensis immune response of the animals, using immuno-diffusion, skin test and macrophage migration inhibition test. It was observed that: 1. Three immunizing doses of the antigens induced a more marked response than two doses; 2. The highest immune response was obtained with the use of Freund's complete adjuvant; 3. Animals challenged a long time (week 6) after the last immunizing dose showed good anti-P. brasiliensis immune response; 4. The particulate antigen induced the lowest immune response. The soluble and the combined antigens were equally efficient in raising good humoral and cellular anti-P. brasiliensis immune response

Effect of Temperature and Antigen Dose on the Antibody Response of Juvenile Coho Salmon (Oncorhynchus kisutch) to Aeromonas salmonicida Endotoxin

1974 ◽  
Vol 31 (11) ◽  
pp. 1743-1749 ◽  
Author(s):  
W. D. Paterson ◽  
J. L. Fryer
Keyword(s):  
Antibody Response ◽  
Aluminum Hydroxide ◽  
Antibody Production ◽  
Specific Antibody ◽  
Intraperitoneal Injection ◽  
Coho Salmon ◽  
Oncorhynchus Kisutch ◽  
Aeromonas Salmonicida ◽  
Effect Of Temperature ◽  
Specific Antibody Production

Specific antibody production in response to an intraperitoneal injection of A. salmonicida endotoxin first occurred in juvenile coho salmon (Oncorhynchus kisutch) held at 6.7, 12.2, and 17.8 C at 4, 2 and 1 wk, respectively. Aeromonas salmonicida endotoxin was antigenic to juvenile coho salmon at all doses between 1 and 5000 μg administered intraperitoneally with and without an aluminum hydroxide carrier.The endotoxin was nontoxic to juvenile coho salmon when 1–5000 μg was injected intraperitoneally and the fish held at temperatures from 6.7 to 17.8 C or when "free endotoxin" was administered by the intramuscular or intraperitoneal route. Aeromonas salmonicida endotoxin was toxic to mice, however.

Development of sporozoite vaccines

1984 ◽  
Vol 307 (1131) ◽  
pp. 117-128 ◽  
Keyword(s):  
Amino Acids ◽  
Monoclonal Antibodies ◽  
Protective Immunity ◽  
Freund’S Complete Adjuvant ◽  
Protein Carriers ◽  
Freund's Complete Adjuvant ◽  
Physico Chemical ◽  
Antigenic Properties ◽  
Peptide Antibodies

Protective immunity against m alaria has been achieved in hosts ranging from birds to man by repeated inoculation of irradiated sporozoites. The main antigens involved in protective immunity to sporozoites are the circumsporozoite (CS) proteins, which are part of a family of proteins, covering the whole surface mem brane of the parasite, and which have similar physico-chemical and antigenic properties. M onovalent fragments of monoclonal antibodies to CS proteins neutralize sporozoite infectivity. All monoclonal antibodies recognize a single im m unodom inant region within the various CS proteins, and this region contains repetitive epitopes. The recurrent im m unodom inant epitope of the CS protein of P. knowlesi has been identified, and shown to consist of 12 tandemly repeated subunits of 12 amino acids. The dimer of the dodecapeptide was coupled to protein carriers, emulsified in Freund’s complete adjuvant, and injected into rodents and monkeys. All animals made anti-peptide antibodies, and most of the antisera reacted with P. knowlesi CS protein.

Virulence and Persistence of Rough and Smooth Forms of Aeromonas salmonicida Inoculated into Coho Salmon (Oncorhynchus kisutch)

1972 ◽  
Vol 29 (2) ◽  
pp. 204-206 ◽  
Author(s):  
Douglas P. Anderson
Keyword(s):  
Coho Salmon ◽  
Oncorhynchus Kisutch ◽  
Aeromonas Salmonicida

Virulent isolates of Aeromonas salmonicida showed a majority of smooth colonies, while the attenuated isolates displayed mostly rough colonies. A lesion occurred at the site of inoculation when one of the rough forms was inoculated into yearling coho salmon, but few mortalities were recorded even though the rough forms were readily recovered from both the lesion and the kidney. The fish inoculated with the same dosage of smooth forms all died within 96 hr of inoculation.

Failure of antisomatostatin antibodies to stimulate the growth of crossbred lambs

1988 ◽  
Vol 28 (2) ◽  
pp. 161 ◽  
Author(s):  
RM Hoskinson ◽  
P Djura ◽  
RJ Welch ◽  
BE Harrison ◽  
GH Brown ◽  
...  
Keyword(s):  
Antibody Response ◽  
Antibody Titre ◽  
Growth Promotion ◽  
Freund’S Complete Adjuvant ◽  
Freund's Complete Adjuvant ◽  
Response Profile

The effect of antisomatostatin antibodies on the growth of crossbred female and wether lambs was studied over 8 months. The antibody response profile established in the immune lambs, following a sequence of 3 vaccinations with an immunogenic somatostatin-haemocyanin conjugate in Freund's complete adjuvant, reached a maximum mean antisomatostatin antibody titre of 1:11 400. There were no effects (P>0.05) of the treatment on liveweight or shoulder height of immune lambs. There were predictable overall effects due to sex but not to level of antibody titre within the immunisation treatment. These data indicate circumstances where immunological growth promotion does not result when antisomatostatin antibodies are raised in growing crossbred lambs that are typical of a commercial breed.

Humoral antibody response to cattle to formalinized Staphylococcus aureus vaccine

1975 ◽  
Vol 21 (11) ◽  
pp. 1756-1759
Author(s):  
T. K. S. Mukkur ◽  
Usha J. Tewari
Keyword(s):  
Staphylococcus Aureus ◽  
Antibody Response ◽  
Post Partum ◽  
Post Inoculation ◽  
Freund’S Complete Adjuvant ◽  
Humoral Antibody ◽  
Freund's Complete Adjuvant ◽  
Agglutination Titer

Five cows were inoculated intradermally with formalinized Staphylococcus aureus suspension in Freund's complete adjuvant and the development of the humoral antibody response was followed as judged by the agglutination titer of sera, at various intervals post inoculation. Highest titers were observed at 78–87 days post inoculation. Agglutinating activity was found in IgM and IgG fractions (IgG1 and IgG2) of both serum and colostrum. The agglutinating activity of colostrum was significantly higher at 12 than at 24 and 36 h, post partum. However, no such activity was detected in either normal cow serum or colostrum against S. aureus.

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