Virucidal Activity of Two Iodophors to Salmonid Viruses

1972 ◽  
Vol 29 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Donald F. Amend ◽  
John P. Pietsch
Keyword(s):  
Organic Matter ◽  
Pancreatic Necrosis ◽  
Water Hardness ◽  
Virucidal Activity ◽  
Viral Hemorrhagic Septicemia ◽  
Organic Iodine ◽  
Infectious Hematopoietic Necrosis ◽  
Infectious Pancreatic Necrosis ◽  
Iodine Complexes

Wescodyne® and Betadine®, organic iodine complexes, were compared in vitro for virucidal activity against infectious hematopoietic necrosis (IHN), infectious pancreatic necrosis (IPN), and viral hemorrhagic septicemia (VHS) viruses. Both iodophors were about equally effective on all three viruses. Each iodophor completely destroyed IHN virus within 30 sec at 12 ppm iodine, and was not affected by water hardness. Virucidal activity, however, was reduced at pH levels above 8.0 and in the presence of organic matter. Wescodyne was also compared with seven disinfectants commonly used in fish hatcheries, for virucidal properties against IHN virus. Wescodyne and chlorine were the only disinfectants to completely destroy the virus. Either Wescodyne or Betadine would effectively destroy the salmonid viruses at less than 25 ppm iodine within 5 min in solutions near neutrality.

Infectious Pancreatic Necrosis Virus in Young Salmonids of the Canadian Maritime Provinces

1969 ◽  
Vol 26 (12) ◽  
pp. 3259-3262 ◽  
Author(s):  
R. M. MacKelvie ◽  
H. Artsob
Keyword(s):  
Tissue Culture ◽  
Atlantic Salmon ◽  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Fish Culture ◽  
Necrosis Virus ◽  
Maritime Provinces ◽  
Viral Hemorrhagic Septicemia ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

Infectious pancreatic necrosis virus was isolated from young salmonids, including Atlantic salmon (Salmo solar), in 9 of the 13 fish culture stations of the Canadian Maritime Provinces. The virus was isolated in tissue culture and identified serologically. There was no evidence of Viral Hemorrhagic Septicemia (Egtved disease).

scholarly journals Expression of VP5 of infectious pancreatic necrosis virus strain VR299 is initiated at the second in-frame start codon

2001 ◽  
Vol 82 (4) ◽  
pp. 805-812 ◽  
Author(s):  
Siegfried Weber ◽  
Dieter Fichtner ◽  
Thomas C. Mettenleiter ◽  
Egbert Mundt
Keyword(s):  
Pancreatic Necrosis ◽  
Nonstructural Protein ◽  
Infectious Pancreatic Necrosis Virus ◽  
Start Codon ◽  
Necrosis Virus ◽  
Virus Growth ◽  
Initiation Of Translation ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

Infectious pancreatic necrosis virus (IPNV), a member of the Birnaviridae with two double-stranded RNA genome segments, encodes five proteins designated VP1 to VP5. To study the function of the 17 kDa nonstructural protein VP5 during virus replication several mutated IPNV genome segments A were constructed and included in a reverse genetics system for IPNV to obtain recombinant virus. Mutations between nt 68 and 85 or nt 94 and 103 in the noncoding region failed to yield viable virus. Only mutations located between nt 86 and 92 and downstream of nt 104 were tolerated, and viable virus could be generated. All IPNV generated showed no difference in replication compared with the wild-type IPNV, indicating that the absence of expression of VP5 did not influence virus growth in vitro. Furthermore, the results presented here indicate that initiation of translation of VP5 occurs at position 113, the second in-frame start codon.

scholarly journals Quantitative Flow Cytometry to Measure Viral Production Using Infectious Pancreatic Necrosis Virus as a Model: A Preliminary Study

2018 ◽  
Vol 8 (10) ◽  
pp. 1734 ◽  
Author(s):  
Diego Vázquez ◽  
Carmen López-Vázquez ◽  
José Olveira ◽  
Isabel Bandín ◽  
Carlos Dopazo
Keyword(s):  
Flow Cytometry ◽  
Viral Replication ◽  
Pancreatic Necrosis ◽  
Polynomial Regression ◽  
Infectious Pancreatic Necrosis Virus ◽  
Necrosis Virus ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus ◽  
Vitro Analysis

In recent decades, flow cytometry (FCM) has become an important tool in virology, due to its applications in viral replication and viral-cell interactions, as well as its capacity to quantify proteins (qFCM). In the present study, we have designed and evaluated a qFCM procedure for the in vitro analysis and quantification of fish viral proteins, using the infectious pancreatic necrosis virus (IPNV) as a model. We have also tested its use for viral titration and adapted the MARIS (method for analysing RNA following intracellular sorting) method for simultaneous quantification of viral RNA expression in infected cells. The procedure has proved to be repeatable and reproducible to an acceptable level, although to ensure reproducibility, the repetition of standard curves is inevitable. Regarding its use for viral quantification, a direct relationship (by a second-degree polynomial regression) between viral titres and Molecules of Equivalent Soluble Fluorochrome (MESF) was observed. Finally, the results support the use of this technology, not only for virus quantification, but also to study viral replication from a quantitative approach.

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