The Effect of in Vitro Passage of Infectious Pancreatic Necrosis Virus (IPNV) on Virulence and Sensitivity of the Virus to Rainbow Trout Serum

2003 ◽  
Vol 15 (2) ◽  
pp. 128-135 ◽  
Author(s):  
Kyoung C. Park ◽  
Paul W. Reno
Keyword(s):  
Rainbow Trout ◽  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Necrosis Virus ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

scholarly journals Replication of Infectious Pancreatic Necrosis Virus in Different Cell Lines and in Rainbow Trout (Oncorhynchus Mykiss) Fingerlings

2014 ◽  
Vol 22 (2) ◽  
pp. 127-133 ◽  
Author(s):  
Natalija Matvienko ◽  
Yury Rud ◽  
Leonid Buchatsky
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Cell Cultures ◽  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Necrosis Virus ◽  
Disease Symptoms ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

Abstract The results of a study of Infectious Pancreatic Necrosis Virus (IPNV) isolated in natural reservoirs in Ukraine are presented. The pathogenicity of isolates was investigated in vitro on cell cultures and in vivo on rainbow trout, Oncorhynchus mykiss (Walbaum), fingerlings. Experimental indications were that the Ukrainian IPNV isolates have affinity with reference European strains. During the reproduction of these isolates in cell cultures of FHM (fat head minnow), RTG-2 (rainbow trout gonads), and BF-2 (bluegill caudal peduncle), complicated degenerative changes were visible that finally led to the full destruction of cell monolayers. The experimental infection of rainbow trout fingerlings resulted in typical disease symptoms that were systemic. However, obvious evidence of viral infection was noted in single individuals only, and the majority of experimental fish died without visible disease symptoms. During the study of physicochemical properties, it was noted that Ukrainian isolates completely lost their infectivity with chloroform treatment and heating to 60°C. This proved that IPNV isolates are resistant to Ion concentrations in the range of pH 3.0 to 12.0.

Sign in / Sign up

Export Citation Format

Share Document