An Improved Extender for Freezing Atlantic Salmon Spermatozoa

1969 ◽  
Vol 26 (12) ◽  
pp. 3254-3258 ◽  
Author(s):  
B. Truscott ◽  
D. R. Idler
Keyword(s):  
Chemical Composition ◽  
Atlantic Salmon ◽  
Propylene Glycol ◽  
Seminal Plasma ◽  
Dimethyl Sulphoxide ◽  
Sperm Cells ◽  
Freeze Thaw ◽  
Simultaneous Addition ◽  
Further Development

A solution of chemical composition based on that of seminal plasma was an effective extender for Atlantic salmon spermatozoa; semen diluted to 20 volumes could be maintained at 4 C in an inactive but potentially motile condition for several days. Propylene glycol at concentrations between 7.0 and 12.5% protected sperm cells against freeze–thaw damage. The addition of glycine or albumin to the basic extender containing 7–12.5% propylene glycol or 10% dimethyl sulphoxide provided an extender meriting further development for the cryopreservation of salmonid spermatozoa. Fertilities of 5–19% were achieved with 10 frozen sperm samples diluted with such extenders. For frozen samples a method of fertilization with simultaneous addition of water and sperm to the eggs is proposed.

scholarly journals Assessment of the possibility of using the method of sludge-lignin dehydration of the Baikal Pulp and Paper Mill by the freeze-thaw method

2018 ◽  
Vol 7 (2.23) ◽  
pp. 114 ◽  
Author(s):  
DI Stom ◽  
GO Zhdanova ◽  
SA Potekhin ◽  
AE Balayan ◽  
MN Saksonov Saksonov ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Physicochemical Properties ◽  
Water Solution ◽  
Organic Fertilizer ◽  
Paper Mill ◽  
Pulp And Paper ◽  
Eisenia Foetida ◽  
Pulp And Paper Mill ◽  
Freeze Thaw ◽  
Principal Possibility

An estimation of the possibility of dehydration of sludge-lignin of the Baikal Pulp and Paper Mill by the freeze-thaw method was carried out. Change of sludge-lignin structure, physicochemical properties and chemical composition after freeze-thaw are shown. Toxicity and chemical composition of the water solution separated from the sludge-lignin during its freeze-thaw is estimated. An increase in the rate of germination of seeds of the cress in the soil with the addition of the frozen-out sludge-lignin in the ratio "sludge-lignin-soil" 1:3-1:4 is shown. Possibility of application and recommendations for the use of freezed out sludge-lignin as an organic fertilizer is considered. Principal possibility of the worm transformation of sludge-lignin, previously dehydrated by the freeze-thaw method, by red Californian worms Eisenia foetida Andrei Bouche (1972) is shown. The issue of prospects for processing this waste in a fertile bio-soil is discussed. The latter can later be used for recultivation of the territory of the sludge accumulator plots of the Baikal Pulp and Paper Mill.  

scholarly journals Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing

2021 ◽  
Vol 8 ◽  
Author(s):  
Eduardo G. Aisen ◽  
Wilfredo Huanca López ◽  
Manuel G. Pérez Durand ◽  
Edita Torres Mamani ◽  
Juan C. Villanueva Mori ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Artificial Insemination ◽  
Reproductive Tract ◽  
Vas Deferens ◽  
Female Reproductive Tract ◽  
Low Fertility ◽  
Sperm Cells ◽  
South American Camelids ◽  
Thawing Process

The viscous seminal plasma (SP) is currently a major impediment to the handling of ejaculate and the development of some biotechnologies in South American camelids. The vas deferens-collected spermatozoa of alpacas is a useful technique to avoid this problem. On the other hand, SP contains a large protein component that has been implicated in the function of spermatozoa within the female reproductive tract. In this sense, the low fertility achieved using transcervical insemination with frozen-thawed spermatozoa in alpacas could be improved by adding SP. This study aimed to evaluate the effect of the whole SP on some in vitro parameters of alpaca spermatozoa after the freezing-thawing-process and the fertility after artificial insemination. It would contribute to a better understanding of the interaction between thawed sperm cells and SP. Spermatozoa were obtained by surgically diverted vas deferens. The samples were diluted with a Tris-based extender, packaged in straws, and frozen. At thawing, each straw was divided into two post-thawing conditions: with the addition of 10% of PBS (control) or with 10% SP (treatment). The sperm cells were evaluated using dynamic parameters, sperm cell morphology, and morphometry. Fertility was assessed by an artificial insemination trial. All in vitro parameters were analyzed by ANOVA. A heterogeneity test was scheduled for the fertility trial. After the freezing-thawing process, motility and plasma membrane functionality was improved when SP was added. No differences were found for post-thaw viability between the control and treatment samples. The percentage of normal cells was higher with SP at post-thawing, and a decrease of the presence of bent tailed spermatozoa with a droplet in the SP group was observed. The length of the head spermatozoa was 3.4% higher in the samples with PBS compared to those in which SP was added. Females pregnant at day 25 post-insemination were 0/12 (with SP inside the straw) and 1/10 (without SP inside the straw). In conclusion, the presence of 10% SP at post-thawing improves sperm cells' motility, functionality, and morphology, indicating that it would be beneficial to improve the frozen-thawed alpaca's physiology spermatozoa. More fertility trials must be developed to increase this knowledge.

scholarly journals Correlation Between Lipid Profile of Sperm Cells and Seminal Plasma With Lipid Profile of Serum in Infertile Men

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Heidar Tavilani ◽  
Akram Vatannejad ◽  
Maryam Akbarzadeh ◽  
Mojgan Atabakhash ◽  
Saeed Khosropour ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Seminal Plasma ◽  
Sperm Cells ◽  
Infertile Men

scholarly journals Chemical composition, antioxidant and antibacterial activities of essential oil of Curcuma phaeocaulis Valeton

2020 ◽  
Vol 49 (3) ◽  
pp. 531-540
Author(s):  
Heng Liang ◽  
Qian Wang ◽  
Chunbang Ding ◽  
Li Zhang ◽  
Ruiwu Yang
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Aerial Part ◽  
Antioxidant Properties ◽  
Volatile Oil ◽  
Antibacterial Activities ◽  
Antibacterial Action ◽  
Underground Part ◽  
Baseline Study ◽  
Further Development

Chemical composition, antioxidant and antibacterial activities of the essential oil of Curcuma phaeocaulis were analysed. In the aerial part, 22 compounds account for 69.23% of the total essential oil and authenticated, while in the underground part, 32 compounds account for 84.28% of the total essential oil and authenticated. Among the compounds detected, α-pinene, camphene, eucalyptol, camphor, borneol, β- tumerone, epi-curzerenone were the main compounds of volatile oil in both parts of Curcuma phaeocaulis. In addition, various lipid antioxidant properties by peroxidation assays, inoxidizability and antibacterial action were assessed. The result may be considered as a baseline study for the further development and application of Curcuma phaeocaulis.

Effects of pulsed electric field on freeze-thaw quality of Atlantic salmon

2020 ◽  
Vol 65 ◽  
pp. 102454 ◽  
Author(s):  
Jiaheng Li ◽  
Jiyong Shi ◽  
Xiaowei Huang ◽  
Xiaobo Zou ◽  
Zhihua Li ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Electric Field ◽  
Pulsed Electric Field ◽  
Freeze Thaw

A Study of Major Cations, Osmotic Pressure, andpH in Seminal Components of Atlantic Salmon

1969 ◽  
Vol 26 (2) ◽  
pp. 413-419 ◽  
Author(s):  
P. C. Hwang ◽  
D. R. Idler
Keyword(s):  
Atlantic Salmon ◽  
Osmotic Pressure ◽  
Seminal Plasma ◽  
Atlantic Cod ◽  
Glucose Solution ◽  
Limited Data ◽  
Ion Balance ◽  
Major Cations ◽  
Cryo Preservation

Certain properties of semen, spermatozoa, and seminal plasma of Atlantic salmon were investigated to facilitate the development of an extender for the cryo-preservation of sperm. The seminal plasma of 10 fish had an average pH of 8.25, an osmotic pressure of 232 milliosmols, and contained 237, 86, 5.2, and 2.2 mg/100 g respectively of Na+, K+, Ca++, and Mg++. The corresponding values for spermatozoa were 84, 298, 1.5, and 18.7. Storage of semen at 2 C caused an increase of Na+and Ca++and a decrease of K+and Mg++in spermatozoa. Subsequent incubation in glucose solution did not restore ion balance in salmon spermatozoa as it did in dog spermatozoa. The results for salmon are compared with limited data for Atlantic cod.

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