Infectious Pancreatic Necrosis: Clinical and Immune Response of Adult Trouts to Inoculation with Live Virus

1969 ◽  
Vol 26 (9) ◽  
pp. 2511-2516 ◽  
Author(s):  
Ken Wolf ◽  
M. C. Quimby
Keyword(s):  
Immune Response ◽  
Brook Trout ◽  
Pancreatic Necrosis ◽  
Neutralizing Antibody ◽  
Asymptomatic Infection ◽  
Live Virus ◽  
Specific Pathogen ◽  
Infectious Pancreatic Necrosis ◽  
Neutralization Index ◽  
Prior Infection

Mature feral rainbow trout were inoculated with infectious pancreatic necrosis (IPN) virus and held at 12.5 C for 3 months to produce antibody and to determine whether or not disease would occur. Disease was not observed, but IPN virus-neutralizing antibody was found in pre-inoculation serum indicating the likelihood of prior infection. As measured by Neutralization Index, the immune response varied from weak to very strong. Neutralizing antibody was not found in sera from mature specific pathogen-free rainbow and brook trout. Although IPN virus also failed to produce clinical disease in adult brook trout it did establish a transient asymptomatic infection, and this resulted in high levels of neutralizing antibody.

scholarly journals Whole-Genome Sequence of Infectious Pancreatic Necrosis Virus Isolated from Farmed Brook Trout ( Salvelinus fontinalis ) in Pennsylvania

2018 ◽  
Vol 6 (17) ◽  
Author(s):  
Kay Palchak ◽  
Shubhada K. Chothe ◽  
Aswathy Sebastian ◽  
Ruth H. Nissly ◽  
Rhiannon Barry ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Brook Trout ◽  
Pancreatic Necrosis ◽  
Systemic Disease ◽  
Fish Farming ◽  
Infectious Pancreatic Necrosis Virus ◽  
Whole Genome Sequence ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus ◽  
Farming Industry

ABSTRACT Infectious pancreatic necrosis (IPN) is an acute contagious systemic disease affecting several fish species and a critical disease in the salmonid fish farming industry. Here, we report the complete genome sequence of IPN virus (IPNV) RNA segments A and B, isolated from a farmed brook trout in Pennsylvania.

Susceptibility of Trouts of Different Species and Origins to Various Isolates of Infectious Pancreatic Necrosis Virus

1982 ◽  
Vol 39 (12) ◽  
pp. 1580-1584 ◽  
Author(s):  
A. Silim ◽  
M. A. S. Y. Elazhary ◽  
A. Lagacé
Keyword(s):  
Rainbow Trout ◽  
Brook Trout ◽  
Pancreatic Necrosis ◽  
Lake Trout ◽  
Infectious Pancreatic Necrosis Virus ◽  
Infected Fish ◽  
Salmo Gairdneri ◽  
Necrosis Virus ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

We investigated the susceptibility of trouts of different species and origins to infectious pancreatic necrosis virus (IPNV) and the pathogenicity of three strains of IPNV for brook trout (Salvelinus fontinalis) of various origins and lake trout (Salvelinus namaycush) of a single origin. Fish were inoculated by immersion in water containing 105 PFU of virus/mL for 6 h. Susceptibility to IPNV infection was assessed by counting dead fish over a period of 21 d after infection and on histological lesions in the pancreas, kidney, and intestine of the infected fish. Different species of trouts had different susceptibility to IPNV strain 3865. Brook trout had the highest mortality followed by rainbow trout (Salmo gairdneri), whereas the least mortality occurred in lake trout. Brook trout from Crowford, Nebraska, were more than twice as susceptible as the same species from Baldwin Mills, Quebec. Rainbow trout also varied in susceptibility as a result of origin. Virus isolate 3B, originally isolated from chain pickerel (Esox niger), was less virulent than isolates 4495 or 3865 (both from trout). Hatcheries that use water from sources containing pickerel may increase their chances of IPN infection.Key words: trout species, infectious pancreatic necrosis virus, resistance

Infectious Pancreatic Necrosis: Selection of Virus-Free Stock from a Population of Carrier Trout

1968 ◽  
Vol 25 (2) ◽  
pp. 383-391 ◽  
Author(s):  
Ken Wolf ◽  
M. C. Quimby ◽  
C. P. Carlson ◽  
G. L. Bullock
Keyword(s):  
Cell Cultures ◽  
Pancreatic Necrosis ◽  
Control Measure ◽  
Effective Control ◽  
Fish Cell ◽  
Specific Pathogen Free ◽  
Trout Population ◽  
Specific Pathogen ◽  
Infectious Pancreatic Necrosis ◽  
Selection Of

Infectious pancreatic necrosis (IPN) is a virulent disease of young trouts and is easily transmitted from infected animals through water and with eggs. At present, the most effective control measure consists of propagation of specific pathogen-free stock. Methods are described for using fish cell cultures to detect IPN virus in peritoneal washes, feces, and gonadal fluids and thereby to identifiy carrier and non-carrier fish. The procedures were used to derive virus-free progeny from a hatchery trout population containing about one-third carrier fish. The progeny were reared to maturity, spawned for 3 successive years, and at each spawning they proved free of virus. Recommendations and precautions are given for those who may wish to apply the procedures.

Outbreak of Infectious Pancreatic Necrosis in Yearling Brook Trout (Salvelinus fontinalis)

1976 ◽  
Vol 33 (11) ◽  
pp. 2621-2625 ◽  
Author(s):  
M. A. S. Y. Elazhary ◽  
A. Lagace ◽  
G. Cousineau ◽  
R. S. Roy ◽  
L. Berthiaume ◽  
...  
Keyword(s):  
Brook Trout ◽  
Pancreatic Necrosis ◽  
Reference Strain ◽  
Salvelinus Fontinalis ◽  
Clinical Signs ◽  
Physicochemical Characteristics ◽  
Genetic Line ◽  
Infectious Pancreatic Necrosis ◽  
Epizootic Disease ◽  
Increased Susceptibility

Studies were conducted to determine the etiology of an epizootic disease occurring in fry and yearling brook trout (Salvelinus fontinalis); clinical signs, experimental infection, and pathological lesions suggested infectious pancreatic necrosis. Electron microscopy and physicochemical characteristics were used to identify the virus isolated from sick trout as IPN virus. The isolates showed only partial antigenic similarity to the reference strain IPN VR299. Increased susceptibility to IPN virus was observed in one genetic line of trout.

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