Infectious Pancreatic Necrosis: Selection of Virus-Free Stock from a Population of Carrier Trout

1968 ◽  
Vol 25 (2) ◽  
pp. 383-391 ◽  
Author(s):  
Ken Wolf ◽  
M. C. Quimby ◽  
C. P. Carlson ◽  
G. L. Bullock
Keyword(s):  
Cell Cultures ◽  
Pancreatic Necrosis ◽  
Control Measure ◽  
Effective Control ◽  
Fish Cell ◽  
Specific Pathogen Free ◽  
Trout Population ◽  
Specific Pathogen ◽  
Infectious Pancreatic Necrosis ◽  
Selection Of

Infectious pancreatic necrosis (IPN) is a virulent disease of young trouts and is easily transmitted from infected animals through water and with eggs. At present, the most effective control measure consists of propagation of specific pathogen-free stock. Methods are described for using fish cell cultures to detect IPN virus in peritoneal washes, feces, and gonadal fluids and thereby to identifiy carrier and non-carrier fish. The procedures were used to derive virus-free progeny from a hatchery trout population containing about one-third carrier fish. The progeny were reared to maturity, spawned for 3 successive years, and at each spawning they proved free of virus. Recommendations and precautions are given for those who may wish to apply the procedures.

Comparative efficacy of cryoprotectant in the lyophilizationof pigeon paramyxovirus-1 vaccine

2017 ◽  
Author(s):  
Mousam Das ◽  
Devi Prasad Isore ◽  
Chanchal Guha ◽  
Ujjwal Biswas ◽  
Amaresh Chatterjee
Keyword(s):  
Polyvinyl Pyrrolidone ◽  
Specific Pathogen Free ◽  
Comparative Efficacy ◽  
Freeze Dried ◽  
Infective Dose ◽  
Specific Pathogen ◽  
First Time ◽  
Lactalbumin Hydrolysate ◽  
Selection Of ◽  
Better Than

Preparation of live attenuated freeze dried Pigeon Paramyxo Virus-1 (PPMV-1) vaccine selection of proper cryoprotectant which could preserve and protect the virus against injury due to reduction of temperature at the time of lyophilization, was very necessary. PPMV-1 locally isolated lentogenic strain was selected as seed virus which was properly attenuated by serially passaging in 9 to 10 days old embryonated specific pathogen free (SPF) fowl eggs and produced master seed virus and working seed virus. Five per cent lactalbumin hydrolysate with 10% sucrose (LAS) and 1% polyvinyl pyrrolidone (PVP) were used as two different cryoprotectant for lyophilization of working seed virus. After lyophilization value of haemagglutination (HA) titer and embryo infective dose fifty per ml (EID50/ml) were changed and concluded that both LAS and PVP could be used as suitable cryoprotectant where LAS was better than PVP which was first time done in the whole world.

scholarly journals Replication of Infectious Pancreatic Necrosis Virus in Different Cell Lines and in Rainbow Trout (Oncorhynchus Mykiss) Fingerlings

2014 ◽  
Vol 22 (2) ◽  
pp. 127-133 ◽  
Author(s):  
Natalija Matvienko ◽  
Yury Rud ◽  
Leonid Buchatsky
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Cell Cultures ◽  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Necrosis Virus ◽  
Disease Symptoms ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

Abstract The results of a study of Infectious Pancreatic Necrosis Virus (IPNV) isolated in natural reservoirs in Ukraine are presented. The pathogenicity of isolates was investigated in vitro on cell cultures and in vivo on rainbow trout, Oncorhynchus mykiss (Walbaum), fingerlings. Experimental indications were that the Ukrainian IPNV isolates have affinity with reference European strains. During the reproduction of these isolates in cell cultures of FHM (fat head minnow), RTG-2 (rainbow trout gonads), and BF-2 (bluegill caudal peduncle), complicated degenerative changes were visible that finally led to the full destruction of cell monolayers. The experimental infection of rainbow trout fingerlings resulted in typical disease symptoms that were systemic. However, obvious evidence of viral infection was noted in single individuals only, and the majority of experimental fish died without visible disease symptoms. During the study of physicochemical properties, it was noted that Ukrainian isolates completely lost their infectivity with chloroform treatment and heating to 60°C. This proved that IPNV isolates are resistant to Ion concentrations in the range of pH 3.0 to 12.0.

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